期刊
SCIENCE SIGNALING
卷 13, 期 647, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scisignal.aaz1436
关键词
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资金
- DFG (Deutsche Forschungsgemeinschaft) [IRTG1816, SFB1002]
- DFG under Germany's Excellence Strategy-EXC [2067/1-390729940]
- DFG [SFB1190]
- Leducq Foundation transatlantic network CURE-PLaN
- Germany's Excellence Strategy-EXC [2067/1-390729940]
- European Commission [675179]
- British Heart Foundation [RG/17/15/33106]
The cardiac membrane protein phospholamban (PLN) is targeted by protein kinase A (PKA) at Ser(16) and by Ca2+/calmodulin-dependent protein kinase II (CaMKII) at Thr(17).beta-Adrenergic stimulation and PKA-dependent phosphorylation of Ser(16) acutely stimulate the sarcoplasmic reticulum calcium pump (SERCA) by relieving its inhibition by PLN. CaMKII-dependent phosphorylation may lead to longer-lasting SERCA stimulation and may sustain maladaptive Ca2+ handling. Here, we demonstrated that phosphorylation at either Ser(16) or Thr(17) converted PLN into a target for the phosphoadaptor protein 14-3-3 with different affinities. 14-3-3 proteins were localized within nanometers of PLN and endogenous 14-3-3 coimmunoprecipitated with pentameric PLN from cardiac membranes. Molecular dynamics simulations predicted different molecular contacts for peptides phosphorylated at Ser(16) or Thr(17) with the binding groove of 14-3-3, resulting in varied binding affinities. 14-3-3 binding protected either PLN phosphosite from dephosphorylation. beta-Adrenergic stimulation of isolated adult cardiomyocytes resulted in the membrane recruitment of endogenous 14-3-3. The exogenous addition of 14-3-3 to 13-adrenergic-stimulated cardiomyocytes led to prolonged SERCA activation, presumably because 14-3-3 protected PLN pentamers from dephosphorylation. Phosphorylation of Ser(16) was disrupted by the cardiomyopathy-associated Delta Arg(14) mutation, implying that phosphorylation of Thr(17) by CaMKII may become crucial for 14-3-3 recruitment to Delta Arg(14)PLN. Consistent with PLN acting as a dynamic hub in the control of Ca2+ handling, our results identify 14-3-3 binding to PLN as a contractility-augmenting mechanism.
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