期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 765, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.142740
关键词
MicroRNA-222; Low dose benzene; DNA repair capacity; MDM2-p53; Challenge assay; Genotoxicity
资金
- National Natural Science Foundation of China [81474929]
- Beijing Natural Science Foundation [7182118]
The study identified genotoxicity in lymphocytes of workers with low exposure doses to benzene, with up-regulation of miR-222 expression inversely associated with DNA repair capacity (DRC). In vitro studies confirmed that exposure to 1,4-benzoquinone (1,4-BQ) increased miR-222 expression and Comet tail length, while decreasing DRC. Loss of miR-222 alleviated DNA damage, but induced S-phase arrest and apoptosis.
Mechanisms for hematotoxicity and health effects from exposure to low doses of benzene (BZ) remain to be identified. To address the information gap, our investigation was focused onto using appropriate populations and cell cultures to investigate novel BZ-induced effects such as disruption of DNA repair capacity (DRC). From our study, abnormal miRNAs were identified and validated using lymphocytes from 56 BZ-poisoned workers and 53 controls. In addition, 173 current BZ-exposed workers and 58 controls were investigated for key miRNA expression using RT-PCR and for cellular DRC using a challenge assay. Subsequently, the observed activities in lymphocytes were verified using human HL-60 (p53 null) and TK6 (p53wild-type) cells via 1,4-benzoquinone (1,4-BQ) treatment and miR-222 interferences. The targeting of MDM2 by miR-222 was validated using a luciferase reporter. Our results indicate induction of genotoxicity in lymphocytes from workers with low exposure doses to BZ. In addition, miR-222 expression was up-regulated among both BZ-poisoned and BZ-exposed workers together with inverse association with DRC. Our in vitro validation studies using both cell lines indicate that 1,4-BQ exposure increased expression of miR-222 and Comet tail length but decreased DRC. Loss of miR-222 reduced DNA damage, but induced S-phase arrest and apoptosis. However, silencing of MDM2 failed to activate p53 in TK6 cells. In conclusion, our in vivo observations were confirmed by in vitro studies showing that BZ/1,4-BQ exposures caused genotoxicity and high expression of miR-222 which obstructed expression of the MDM2-p53 axis that led to failed activation of p53, abnormal DRC and serious biological consequences. (C) 2020 Elsevier B.V. All rights reserved.
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