Article
Virology
Dan David, Nick Storm, Waksman Ilan, Asaf Sol
Summary: This study isolated and characterized local BCoV strains in Israel, finding that they belong to different genetic variants and cluster together with other BCoV circulating in the area. These local strains are genetically close to other BCoV strains in the region but distant from the reference enteric strain Mebus.
Review
Biochemistry & Molecular Biology
David E. Scheim, Paola Vottero, Alessandro D. Santin, Allen G. Hirsh
Summary: According to biochemical properties, sialylated glycan attachments between SARS-CoV-2 spike protein and host cells play a key role in the virus's pathology. The virus can attach to and damage red blood cells, platelets, and endothelial cells. Risk factors for severe COVID-19, such as age, diabetes, and obesity, are associated with increased RBC clumping. Furthermore, differences in glycan attachment release between common cold strains and deadly coronaviruses have been identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Wakana Saso, Masako Yamasaki, Shin-ichi Nakakita, Shuetsu Fukushi, Kana Tsuchimoto, Noriyuki Watanabe, Nongluk Sriwilaijaroen, Osamu Kanie, Masamichi Muramatsu, Yoshimasa Takahashi, Tetsuro Matano, Makoto Takeda, Yasuo Suzuki, Koichi Watashi
Summary: This study identified that alpha 2-6-linked sialic acid-containing compounds play a significant role in the efficient spread of SARS-CoV-2 infection, while this was not the case with SARS-CoV. The binding of alpha 2-6-linked compounds to the SARS-CoV-2 spike S1 subunit competitively inhibits the attachment of SARS-CoV-2 to host cells. Removal of cell surface sialic acids impairs the interaction between the SARS-CoV-2 spike and angiotensin-converting enzyme 2 (ACE2), leading to the suppression of SARS-CoV-2 infection. This study provides a molecular basis for understanding the distinct characteristics of SARS-CoV-2 compared to SARS-CoV.
Review
Biochemistry & Molecular Biology
Wenjuan Du, Erik de Vries, Frank J. M. van Kuppeveld, Mikhail Matrosovich, Cornelis A. M. de Haan
Summary: Human influenza A viruses originate from animal viruses, with HA and NA playing important roles in the virus particles, although the role of NA in host adaptation is less understood. NA plays a crucial role in adjusting the HA-NA balance of virus particles and affecting virus replication, likely influencing the viral host range.
Article
Virology
Josanne H. Verhagen, Per Eriksson, Lonneke Leijten, Ola Blixt, Bjorn Olsen, Jonas Waldenstrom, Patrik Ellstrom, Thijs Kuiken
Summary: This study shows that Mallards play a significant role in the ecology and epidemiology of IAV, with abundant attachment to the colon and a preference for fucosylated glycan structures. Further understanding of virus tissue attachment and receptor binding is crucial for comprehending IAV host range and epidemiology.
JOURNAL OF VIROLOGY
(2021)
Article
Chemistry, Multidisciplinary
Casia L. Wardzala, Amanda M. Wood, David M. Belnap, Jessica R. Kramer
Summary: Mucins play an important role in coronavirus transmission, and their molecular structure plays a complex and significant role in host defense.
ACS CENTRAL SCIENCE
(2022)
Article
Microbiology
Hataf Khan, Helena Winstone, Jose M. Jimenez-Guardeno, Carl Graham, Katie J. Doores, Caroline Goujon, David A. Matthews, Andrew D. Davidson, Suzannah J. Rihn, Massimo Palmarini, Stuart J. D. Neil, Michael H. Malim
Summary: The IFN-inducible short isoform of human nuclear receptor coactivator 7 (NCOA7) inhibits virus entry by promoting endo-lysosomal vesicle acidification, and is effective against SARS-CoV-2 and other coronaviruses. The acquisition of furin cleavage may have favored the utilization of cell surface TMPRSS proteases as a strategy to evade the suppressive effects of IFN-induced endo-lysosomal dysregulation on virus infection.
Article
Chemistry, Multidisciplinary
Guillermo Moreno-Pescador, Mohammad Reza Arastoo, Victoria Thusgaard Ruhoff, Salvatore Chiantia, Robert Daniels, Poul Martin Bendix
Summary: Many cellular processes involve the lateral organization of membrane proteins into nanoscale domains. The mechanisms behind the clustering of membrane proteins into nanoscale lipid domains are not well understood. In this study, a method combining optical trapping, thermo-plasmonic-mediated membrane fusion, and confocal imaging was developed to investigate the phase affinity of membrane proteins. It was observed that the transferred membrane proteins partitioned into the liquid disordered phase of the model membranes. This generic platform allows the study of phase affinity for any plasma membrane protein labeled with a fluorescent marker.
Article
Chemistry, Multidisciplinary
Elizabeth R. Webster, Katherine N. Liu, Robert J. Rawle, Steven G. Boxer
Summary: This study investigates the impact of receptor length and rigidity on the mechanism of influenza virus entry. The results suggest that shorter and more flexible receptors increase the binding rate of the virus, while longer and more rigid receptors decrease the efficiency of lipid and content mixing.
Article
Chemistry, Analytical
Hao Chang, Yu Mei, Yidan Li, Luqing Shang
Summary: In this study, a novel neuraminidase fluorescent probe with aggregation-induced emission and excited state intramolecular proton transfer activity was developed for influenza virus detection. The probe showed high sensitivity and strong fluorescence signal towards neuraminidase, making it suitable for influenza virus detection and living cell imaging. It was also able to distinguish oseltamivir-resistant influenza virus from wild type.
Article
Biochemistry & Molecular Biology
David E. Scheim
Summary: Rouleaux of red blood cells were observed in the blood of COVID-19 patients, which is related to the properties of several enveloped virus strains. SARS-CoV-2 can bind to red blood cells and attach to sialic acid moieties through the glycans on its spike protein. Additionally, certain enveloped viruses have an enzyme that can release glycan-mediated bindings, but this enzyme is not expressed in the COVID-19 strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Kadir Yesilbag, Eda Baldan Toker, Ozer Ates
Summary: This study investigated the presence of IDV in nasal swab samples collected from cattle with BRD between 2012 and 2021, and identified the circulation of D/Yama2019 and D/Bursa2013 (a new lineage) in Turkey. The study also highlighted the differences in nucleotide and amino acid substitutions among different lineages of IDV.
MICROBIAL PATHOGENESIS
(2022)
Review
Immunology
Xi Jiang, Ming Tan, Ming Xia, Pengwei Huang, Michael A. Kennedy
Summary: The mechanisms behind the pathogenicity of the COVID-19 pandemic and the 1918 H1N1 influenza pandemic viruses remain enigmatic. Researchers have proposed the impact of host sialic acid receptor polymorphisms on virus infectivity, and discussed common principles of evolution between influenza viruses and coronaviruses.
EMERGING MICROBES & INFECTIONS
(2021)
Review
Virology
Holly Everest, Phoebe Stevenson-Leggett, Dalan Bailey, Erica Bickerton, Sarah Keep
Summary: This article reviews the interactions between animal and human coronaviruses and their cellular receptors, emphasizing the importance of understanding these interactions for disease prevention and intervention strategies. The role of receptor-binding and glycan-coronavirus interactions in infection, tropism, and pathogenesis is highlighted, with a focus on the need for continued research in this area.
Article
Virology
Cindy M. Spruit, Xueyong Zhu, Ilhan Tomris, Maria Rios-Carrasco, Alvin X. Han, Frederik Broszeit, Roosmarijn van der Woude, Kim M. Bouwman, Michel M. T. Luu, Keita Matsuno, Yoshihiro Sakoda, Colin A. Russell, Ian A. Wilson, Geert-Jan Boons, Robert P. de Vries
Summary: Influenza A viruses cause millions of severe illnesses and deaths each year. This study investigates the molecular determinants of NeuGc specificity and the origin of viruses that bind NeuGc.
JOURNAL OF VIROLOGY
(2022)
Article
Chemistry, Multidisciplinary
Dushen Chen, Akhilesh. K. K. Srivastava, Justyna Dubrochowska, Lin Liu, Tiehai Li, Joseph. P. P. Hoffmann, Jay. K. K. Kolls, Geert-Jan Boons
Summary: There is a pressing need for new treatment options for carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae), a common cause of life-threatening infections. In this study, a synthetic glycoconjugate vaccine was developed using oligosaccharides derived from the lipopolysaccharide of K. pneumoniae. Mice immunized with this vaccine showed a strong antibody response against K. pneumoniae.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Microbiology
David Scheibner, Ahmed H. Salaheldin, Ola Bagato, Luca M. Zaeck, Ahmed Mostafa, Ulrike Blohm, Christin Mueller, Ahmed F. Eweas, Kati Franzke, Axel Karger, Alexander Schaefer, Marcel Gischke, Donata Hoffmann, Solene Lerolle, Xuguang Li, Hatem S. Abd El-Hamid, Jutta Veits, Angele Breithaupt, Geert-Jan Boons, Mikhail Matrosovich, Stefan Finke, Stephan Pleschka, Thomas C. Mettenleiter, Robert P. de Vries, Elsayed M. Abdelwhab
Summary: Mutations in the neuraminidase (NA) of human H5N1 viruses play different roles in infection of mammals without affecting virulence or transmission in chickens. This study is important to understand the genetic determinants for replication of avian influenza viruses in mammals and should assist in the prediction of avian influenza viruses with zoonotic potential.
Article
Biochemistry & Molecular Biology
Ilhan Tomris, Luca Unione, Linh Nguyen, Pouya Zaree, Kim M. Bouwman, Lin Liu, Zeshi Li, Jelle A. Fok, Maria Rios Carrasco, Roosmarijn van der Woude, Anne L. M. Kimpel, Mirte W. Linthorst, Sinan E. Kilavuzoglu, Enrico C. J. M. Verpalen, Tom G. Caniels, Rogier W. Sanders, Balthasar A. . Heesters, Roland J. Pieters, Jesu's Jimenez-Barbero, John S. Klassen, Geert-Jan Boons, Robert P. de Vries
Summary: SARS-CoV-2 viruses use ACE2 as a functional receptor through their spike protein. The NTD of SARS-CoV-2 beta variant strain is capable of binding to Vero E6 cells and has a specific preference for 9-O-acetylated structures, suggesting a potential dual-receptor functionality of the S1 domain.
ACS CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Analytical
Joshua C. L. Maliepaard, J. Mirjam A. Damen, Geert-Jan P. H. Boons, Karli R. Reiding
Summary: Glycosylation is a vital protein modification on extracellular human proteins which is analyzed with mass spectrometry. In this study, a LC-MS/MS-based workflow was developed to determine glycopeptide isomer ratios. The method could quantify isomerism within mixtures and was independent from the peptide portion of the conjugate.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Gael M. Vos, Julia Weber, Igor R. Sweet, Kevin C. Hooijschuur, Javier Sastre Torano, Geert-Jan Boons
Summary: Protein O-glycosylation is a highly diverse type of post-translational modification. The release of glycans from glycoconjugates is a crucial step in the analysis of O-glycomes. Researchers have developed a robust workflow for the oxidative release of O-glycans using synthetic glycopeptides, which allows for the retention of unique amino acid information and labile modifications.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Ming Xia, Frank Vago, Ling Han, Pengwei Huang, Linh Nguyen, Geert-Jan Boons, John S. Klassen, Wen Jiang, Ming Tan
Summary: In this study, the aTSR domain of the CSP was characterized and found to bind to heparan sulfate glycans, making it an important functional domain and a potential vaccine target. Fusion of aTSR with norovirus VP1's S domain resulted in the formation of highly immunogenic nanoparticles that elicited specific antibodies. These findings suggest that the aTSR nanoparticle could be a promising vaccine candidate for preventing and treating malaria.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Jerome Riedel, Gerard Meijer, Gert von Helden, Maike Lettow, Michael Gotze, Rebecca L. Miller, Geert-Jan Boons, Gergo Peter Szekeres, Kevin Pagel, Marko Grabarics
Summary: Glycosaminoglycans (GAGs), known for their diverse structures and importance in physiological processes, have gained attention in biochemical and biomedical research. Cryogenic gas-phase infrared (IR) spectroscopy has shown promise in identifying structural motifs of shorter GAG chains. This study used mass-selected heparan sulfate (HS) ions to extract characteristic vibrational features and employed random forest (RF) classifiers to discriminate between GAG classes and sulfate positions. The combination of gas-phase cryogenic IR ion spectroscopy and machine learning achieved high prediction accuracy for HS tetra- and hexasaccharides, demonstrating its importance in improving GAG sequencing and analysis of biomolecules.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Renuka Kadirvelraj, Bhargavi M. Boruah, Shuo Wang, Digantkumar Chapla, Chin Huang, Annapoorani Ramiah, Kieran L. Hudson, Anthony R. Prudden, Geert-Jan Boons, Stephen G. Withers, Zachary A. Wood, Kelley W. Moremen
Summary: Mammalian cell surface and secreted glycoproteins exhibit diverse glycan structural diversity, including Lewis antigens synthesized by GT10 family. This study determined the crystal structures of human FUT9, an a1,3-fucosyltransferase, revealing substrate specificity determinants and providing a catalytic model. Comparisons with other GT10 fucosyltransferases and GT-B fold glycosyltransferases suggest modular evolution and specificity for Lewis antigen synthesis among mammalian GT10 fucosyltransferases.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Cell Biology
Hailee R. Perrett, Philip J. M. Brouwer, Jonathan Hurtado, Maddy L. Newby, Lin Liu, Helena Mueller-Kraeuter, Sarah Mueller Aguirre, Judith A. Burger, Joey H. Bouhuijs, Grace Gibson, Terrence Messmer, John S. Schieffelin, Aleksandar Antanasijevic, Geert-Jan Boons, Thomas Strecker, Max Crispin, Rogier W. Sanders, Bryan Briney, Andrew B. Ward
Summary: This study presents the development and characterization of prefusion-stabilized GPCs from different lineages of Lassa virus. It reveals structural conservation and neutralization mechanisms of antibodies, and identifies a potential neutralizing antibody, providing important molecular information for vaccine design against Lassa fever.
Article
Multidisciplinary Sciences
Gael M. Vos, Kevin C. Hooijschuur, Zeshi Li, John Fjeldsted, Christian Klein, Robert P. de Vries, Javier Sastre Torano, Geert-Jan Boons
Summary: In this study, a method was developed to determine the structures of sialic acid variants and investigate their roles in biological and disease processes. The method utilized a library of synthetic sialic acids to establish collision cross section values of diagnostic fragment ions and analyzed sialic acids from biological samples to uncover their linkage types and acetylation patterns.
NATURE COMMUNICATIONS
(2023)
Review
Behavioral Sciences
Xiaoyue Ma, Hanling Liu, Qianqian Jia, Yumiao Zheng, Wentao Li, Mengyu Chang, Haixia Fu, Hongmei Zhu
Summary: Glucocorticoids (GCs) serve as endocrine hormones and play a crucial role in various physiological processes, including mammary growth and lactation, circulatory metabolism, and responses to external stimuli. This review highlights the impact of GCs changes on ruminant mammary gland function and disease, emphasizing the modulation of mammary gland development by GCs and the negative effect of stress-induced excessive release of GCs on milk production. The review also discusses the potential therapeutic use of exogenous GCs in alleviating mastitis symptoms.
STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS
(2023)
Article
Veterinary Sciences
Yaofang Hu, Changsheng Jiang, Yueqiao Zhao, Hua Cao, Jingping Ren, Wei Zeng, Mengjia Zhang, Yongtao Li, Qigai He, Wentao Li
Summary: In this study, the TurboID proximity labeling system was used to identify and study host proteins in iPAM cells that interact with the ApxI toxin of APP. Mass spectrometry identified 318 unique interacting proteins, with only one membrane protein CAV1 being identified. Co-immunoprecipitation confirmed the interaction between CAV1 and ApxIA. These findings provide valuable insights for the clinical treatment of PCP.
VETERINARY RESEARCH
(2023)
Article
Virology
Yue Wang, Huimin Huang, Dongliang Li, Chenxu Zhao, Shuai Li, Panpan Qin, Yaqin Li, Xia Yang, Wenjuan Du, Wentao Li, Yongtao Li
Summary: In this study, a recombinant PEDV expressing renilla luciferase was used to screen potential anti-PEDV agents from an FDA-approved drug library, and four compounds were identified. Among them, niclosamide showed the most potent antiviral activity and the highest selectivity index, and it could efficiently inhibit viral RNA synthesis, protein expression, and viral progeny production. Additionally, a combination with other small molecule inhibitors of endosomal acidification enhanced the anti-PEDV effect of niclosamide.
Article
Biochemistry & Molecular Biology
Xinyue Zhou, Mengjia Zhang, Hanyu Zhang, Hailong Ma, Jiaru Zhou, Hua Cao, Guanghao Guo, Ningning Ma, Qigai He, Yongle Yang, Yifei Lang, Yaowei Huang, Wentao Li
Summary: This study successfully generated and purified six monoclonal antibodies specifically targeting the spike protein of SADS-CoV. Three of these antibodies were capable of neutralizing SADS-CoV infection, and it was found that the hemagglutination phenomenon induced by SADS-CoV could be effectively suppressed by monoclonal antibodies specifically targeting the S1A domain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)