期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 117, 期 37, 页码 23001-23010出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2006534117
关键词
population-state decision; intelligently reprogramming; quorum sensing; extracellular electron transfer (EET); Cr (VI) reduction
资金
- National Natural Science Foundation of China [21590812, 21806160, 51821006]
- National Key Research and Development Program of China [2018YFA0901301, 2018YFC0406303]
- International Partnership Program of Chinese Academy of Sciences [GJHZ1845]
- Program for Changjiang Scholars and Innovative Research Team in University of the Ministry of Education of China
- Fundamental Research Funds for the Central Universities [WK 2060000002]
The unique extracellular electron transfer (EET) ability has positioned electroactive bacteria (EAB) as a major class of cellular chassis for genetic engineering aimed at favorable environmental, energy, and geoscience applications. However, previous efforts to genetically enhance EET ability have often impaired the basal metabolism and cellular growth due to the competition for the limited cellular resource. Here, we design a quorum sensing-based population-state decision (PSD) system for intelligently reprogramming the EET regulation system, which allows the rebalanced allocation of the cellular resource upon the bacterial growth state. We demonstrate that the electron output from Shewanella oneidensis MR-1 could be greatly enhanced by the PSD system via shifting the dominant metabolic flux from initial bacterial growth to subsequent EET enhancement (i.e., after reaching a certain population-state threshold). The strain engineered with this system achieved up to 4.8-fold EET enhancement and exhibited a substantially improved pollutant reduction ability, increasing the reduction efficiencies of methyl orange and hexavalent chromium by 18.8- and 5.5-fold, respectively. Moreover, the PSD system outcompeted the constant expression system in managing EET enhancement, resulting in considerably enhanced electron output and pollutant bioreduction capability. The PSD system provides a powerful tool for intelligently managing extracellular electron transfer and may inspire the development of new-generation smart bioelectrical devices for various applications.
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