Review
Infectious Diseases
Mengyu Chen, Rundong Qin, Mei Jiang, Zhaowei Yang, Weiping Wen, Jing Li
Summary: Serological antibody tests play a supplemental role in diagnosing COVID-19, with tests conducted 2 weeks after symptom onset showing better diagnostic accuracy. Detection of S protein as the antigen and chemiluminescence immunoassay could offer more accurate results.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Virology
Yutaro Kitagawa, Kazuo Imai, Masaru Matsuoka, Ai Fukada, Katsumi Kubota, Momoko Sato, Tomohito Takada, Sakiko Noguchi, Norihito Tarumoto, Shigefumi Maesaki, Shinichi Takeuchi, Takuya Maeda
Summary: Fully automated immunoassays for detecting SARS-CoV-2 antibodies showed high sensitivity and specificity for measuring neutralization antibodies in COVID-19 patients. The correlation between these tests and neutralizing antibodies was strong, suggesting that they could be alternatives for assessing humoral immunity.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Zu-Li Zhang, Yu-Lei Hou, De-Tao Li, Feng-Zeng Li
Summary: The detection of anti-SARS-CoV-2 IgG and IgM showed high diagnostic efficiency in assisting the diagnosis of SARS-CoV-2 infection, with gold immunochromatography assay potentially being the preferred method.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Immunology
Hannah A. D. King, Vincent Dussupt, Letzibeth Mendez-Rivera, Bonnie M. M. Slike, Ursula Tran, Nathan D. D. Jackson, Erica Barkei, Michelle Zemil, Emily Tourtellott-Fogt, Caitlin H. H. Kuklis, Sandrine Soman, Aslaa Ahmed, Maciel Porto, Christopher Kitajewski, Brittany Spence, Dalia Benetiene, Lindsay Wieczorek, Swagata Kar, Gregory Gromowski, Victoria R. R. Polonis, Shelly J. J. Krebs, Kayvon Modjarrad, Diane L. L. Bolton
Summary: This study investigates the protective effects of convalescent immunoglobulins against SARS-CoV-2 in a Syrian golden hamster model. It shows that IgM has higher neutralization potency than IgG, but fails to protect hamsters from disease. IgG infusion, on the other hand, effectively prevents disease in a dose-dependent manner, with detectable neutralizing titers correlating with protection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ana Carolina Serafim Vilela, Camila Alves Costa, Suzane Aparecida Oliveira, Menira Borges Lima Dias Souza, Fabiola Souza Fiaccadori, Claudio Rodrigues Leles, Nadia Lago Costa
Summary: The study assessed the accuracy of three rapid tests for detecting SARS-CoV-2 antibodies in saliva and plasma samples. The tests showed higher accuracy in plasma samples. Saliva can be used as a simple supplementary method, especially in severe and critical cases, with important sensitivity and reliability.
Article
Medical Laboratory Technology
Nan Young Lee, Ji Yeon Ham, Narae Hwang, Kyung Eun Song
Summary: With the rapid spread of COVID-19, there is an increasing need for quick and accurate detection of the virus for disease prevention and control. This study evaluated the performance of two rapid tests, a chromatographic immunoassay and a fluorescent immunoassay, in qualitatively detecting specific antibodies, IgM and IgG to SARS-CoV-2 in capillary blood samples.
CLINICAL LABORATORY
(2023)
Article
Chemistry, Analytical
Haifeng Liu, Erhei Dai, Rui Xiao, Zihui Zhou, Minli Zhang, Zikun Bai, Ying Shao, Kezong Qi, Jian Tu, Chongwen Wang, Shengqi Wang
Summary: This study reported a surface-enhanced Raman scattering-based lateral flow immunoassay method for the high sensitivity simultaneous detection of anti-SARS-CoV-2 IgM/IgG. The method utilized custom SERS tags and dual layers of Raman dye, with signal intensities easily recorded by a portable Raman instrument for high sensitivity analysis. Validation on samples from COVID-19 patients and healthy individuals showed high accuracy and specificity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Immunology
Xuzhen Qin, Jun Shen, Erhei Dai, Haolong Li, Guodong Tang, Lixia Zhang, Xin Hou, Minya Lu, Xian Wu, Simeng Duan, Jingjia Zhang, Man-Fung Tsoi, Ping Jiang, Yongzhe Li
Summary: This study aimed to understand the kinetics of IgM and IgG SARS-CoV-2 antibodies in COVID-19 patients and the association between clinical classification and antibodies. The results showed that the seroprevalence of SARS-CoV-2 IgM and IgG in COVID-19 patients was over 70% within 7 days of symptom onset, and the peak concentrations of IgM and IgG were reached on the 10th and 20th days after symptom onset.
Article
Chemistry, Analytical
Qiaoge Bayin, Lei Huang, Chunhui Ren, Yusheng Fu, Xing Ma, Jinhong Guo
Summary: The study introduces a novel lateral flow immunoassay method based on superparamagnetic nanoparticles and giant magnetoresistance sensing system for simultaneous quantitative detection of anti-SARS-CoV-2 immunoglobulin M and G. This method offers advantages in cost-effectiveness, time efficiency, and ease of operation.
Article
Virology
Shun Kaneko, Yoko Nukui, Takeshi Arashiro, Yoshibumi Aiso, Maya Sugii, Yoshiro Hadano, Kaoru Nagata, Reiko Taki, Ken Ueda, Satoko Hanada, Shinichiro Suzaki, Naoshige Harada, Yoshimi Yamaguchi, Hiroyuki Nakanishi, Masayuki Kurosaki, Masayuki Nagasawa, Namiki Izumi
Summary: This study evaluated the clinical performance of the colloidal gold immunochromatography assay for SARS-CoV-2 IgM/IgG antibodies in COVID-19 patients, showing a high sensitivity and specificity for the diagnosis of the disease.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Chemistry, Analytical
Zhitong Zhu, Xiaoming Ma, Lin Zhu, Qiong Luo, Nan Lin, Zhonghui Chen, Xingchen Zhao, Zhenyu Lin, Zongwei Cai
Summary: COVID-19 continues to pose a major threat to human society. A reliable and rapid assay for serum neutralizing antibodies is necessary for pandemic management. Using gold nanoparticles as an immunosensor, a semi-quantitative, instrument-free assay for measuring COVID-19 antibody levels was developed. The method showed good correlation with conventional ELISA assays and could potentially be used for on-site measurement of COVID-19 antibodies.
Article
Biochemical Research Methods
Helene Pere, Ralph-Sydney Mboumba Bouassa, Serge Tonen-Wolyec, Isabelle Podglajen, David Veyer, Laurent Belec
Summary: This study compared the sensitivity and specificity of five commercially available SARS-CoV-2 whole-blood finger-stick IgG/IgM combined rapid diagnostic tests. The results showed that these tests had high sensitivity for screening individuals previously infected by SARS-CoV-2, but some tests lacked specificity, posing a risk of false positivity.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Immunology
Mohd Raeed Jamiruddin, Md Ahsanul Haq, Kazuhito Tomizawa, Eiry Kobatake, Masayasu Mie, Sohel Ahmed, Shahad Saif Khandker, Tamanna Ali, Nowshin Jahan, Mumtarin Jannat Oishee, Mohib Ullah Khondoker, Bijon Kumar Sil, Mainul Haque, Nihad Adnan
Summary: This study observed antibody dynamics in individuals exposed to SARS-COV-2, finding that COVID-19 patients experienced more severe symptoms during their second episode, accompanied by a significant increase in IgG antibodies. IgM and IgA antibodies in the blood may wane quickly, while IgG antibodies persist. The findings suggest potential implications for the development of sero-diagnostics and predictions for ongoing vaccination efforts.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Virology
Weiping Liu, Xia Long, Kexing Wan, Minggang Yin, Yi Yin, Bo Zhang, Lin Li, Yaohui Song
Summary: This study investigated the endogenous interference factors of novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM/IgG detection results. The results showed that factors such as rheumatoid factor (RF) positive, antinuclear antibody (ANA) positive, pregnant women, and normal seniors can lead to false-positive reactivity of SARS-CoV-2 IgM and/or IgG detected using enzyme-linked immunosorbent assay (ELISA).
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Microbiology
Yalcin Pisil, Zafer Yazici, Hisatoshi Shida, Tomoyuki Miura
Summary: Recent research has shown that IgM and IgA monoclonal antibodies have higher neutralization efficiency against the SARS-CoV-2 spike protein compared to IgG monoclonal antibodies, suggesting a new strategy for developing antibody therapies for COVID-19.