Article
Biochemistry & Molecular Biology
Masakatsu Watanabe
Summary: This study investigated the role of two SR residues in the Cx39.4 gene in zebrafish skin patterning. The results showed that these SR residues are critical for channel function, leading to stripe pattern formation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2023)
Article
Biochemistry & Molecular Biology
Erin M. O'Shaughnessy, William Duffy, Laura Garcia-Vega, Keith Hussey, A. David Burden, Mozheh Zamiri, Patricia E. Martin
Summary: Elevated expression of Connexin26 in psoriatic tissue may contribute to cellular events imbalance, while inhibition of Connexin signaling reduces pro-inflammatory events and may have therapeutic benefits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Natalia Kasica, Piotr Jakubowski, Jerzy Kaleczyc
Summary: The study demonstrated that TQR has a dose-dependent effect on increasing the area covered with melanophores while reducing the area covered by iridophores in zebrafish. Additionally, TQR was found to enhance melanophore renewal after depigmentation caused by treatment. Furthermore, gene expression analysis revealed differential patterns in pigment development genes upon exposure to TQR.
Article
Chemistry, Multidisciplinary
Jiyan Qiao, Rui Chen, Mengjie Wang, Ru Bai, Xuejing Cui, Ying Liu, Chongming Wu, Chunying Chen
Summary: The research investigated the toxic effects of different types of M/NPLs on mice, showing that oral administration of these M/NPLs caused gut injuries and decreased expression of tight junction proteins, with toxicity ranking as PS-NH2 > PS-COOH > pristine PS. The study also found that M/NPLs led to gut microbiota dysbiosis, with genera containing opportunistic pathogens and deteriorating intestinal barrier function, highlighting the important indirect toxic effect of gut microbiota dysbiosis in M/NPLs-induced gut barrier dysfunction.
Review
Cell Biology
Stefan Dhein, Aida Salameh
Summary: The heart relies on gap junction channels to maintain cell-cell coupling for efficient and rapid electrical conduction. Connexin expression and gap junction localization play crucial roles in modulating cardiac electrical properties, ultimately affecting cardiac stability and function.
Article
Plant Sciences
Wintai Kamolsukyeunyong, Wissarut Sukhaket, Kitsada Pitija, Pornwalai Thorngkham, Sugunya Mahatheeranont, Theerayut Toojinda, Apichart Vanavichit
Summary: The rice sesquiterpene synthase II gene plays a role in the antixenosis defense mechanism against brown planthopper infestation, with differences in functionality observed between resistant and susceptible rice varieties. Single-nucleotide polymorphisms in the gene's promoter region and exon 5, as well as a seven amino acid deletion in the protein sequence, may negatively regulate gene function. Analysis suggests a correlation between SNPs in specific genes and the expression of OsSTPS2, while a 21 bp deletion in exon 5 affects the antixenosis mechanism during BPH infestation.
Article
Cell & Tissue Engineering
Yiwei Yang, Xing Wang, Yu Tan, Yanxin Xu, Xudong Guo, Yukang Wu, Wuchan Wang, Ruiqi Jing, Fugui Zhu, Dan Ye, Qingquan Zhang, Chenqi Lu, Jiuhong Kang, Guiying Wang
Summary: As crucial epigenetic regulators, long noncoding RNAs (lncRNAs) play critical functions in development processes and various diseases. In this study, the researchers identified a lncRNA called LncCMRR which is involved in early heart development. Knockout of LncCMRR decreased the formation of cardiac mesoderm and cardiomyocytes in mouse embryonic stem cells.
Review
Physiology
Hiroyuki Yamakawa, Tomoko S. Kato, Jaeduk Yoshimura Noh, Shinsuke Yuasa, Akio Kawamura, Keiichi Fukuda, Yoshiyasu Aizawa
Summary: Thyroid hormones have various effects on the cardiovascular system, including regulating heart rate, cardiac contractility, and vascular resistance. Researchers have studied the molecular pathways of TH action in the cardiovascular system, including genomic and non-genomic pathways. This review summarizes the impact of THs on cardiac function, treatment principles, and other aspects related to heart function.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Medicine, General & Internal
Wen Li, Xiaoyang Huang, Dan Bi
Summary: This study investigates the role and mechanism of miRNA-21 in necrotizing enterocolitis (NEC). Clinical data shows that the expression of miRNA-21 gene is significantly upregulated in NEC children. In rat experiments, overexpression of miRNA-21 improves the pathology and cell apoptosis in colonic tissue and significantly affects the expression levels of related proteins. miRNA-21 may serve as a biological marker and therapeutic target in NEC diagnosis and treatment.
ARCHIVES OF MEDICAL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Antonio Rodriguez-Sinovas, Jose Antonio Sanchez, Laura Valls-Lacalle, Marta Consegal, Ignacio Ferreira-Gonzalez
Summary: Connexins are transmembrane proteins that are crucial for cardiac physiology, facilitating electrical coupling between cardiomyocytes. Beyond cell-to-cell communication, connexins also play roles in cell death and survival, with additional functions such as opening of hemichannels and involvement in cardioprotection and cell growth. In-depth exploration of connexin biology sheds light on their implications in various aspects of cardiac pathophysiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Xiaoli Jin, Jian Chen, Asadullah Khan, Ziyan Chen, Rui Gao, Yingying Lu, Xi Zheng
Summary: In this study, the OsSG34 gene was identified to play an important role in rice grain appearance by regulating lipid metabolism and cell wall components. The gene affects grain size and quality by influencing cell proliferation, expansion, and starch content. These findings have significant implications for rice breeding using gene-editing and marker-assisted selection.
Article
Cell Biology
V. Praveen Chakravarthi, Subhra Ghosh, Sami M. Housami, Huizhen Wang, Katherine F. Roby, Michael W. Wolfe, William H. Kinsey, M. A. Karim Rumi
Summary: Ovarian KISS1 signaling plays a crucial role in gonadotropin-induced follicle development and oocyte maturation.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Jing Liu, Kathryn A. Salvati, Scott C. Baraban
Summary: STXBP1 mutations are linked to various neurological disorders, and abnormal neural network activity under this mutation could play a key role in epileptic network formation. Using a zebrafish model combined with calcium imaging and electrophysiology recordings helps uncover this process.
Article
Engineering, Biomedical
Hekai Li, Wenyu Ye, Bin Yu, Xin Yan, Yuhui Lin, Jie Zhan, Peier Chen, Xudong Song, Pingzhen Yang, Yanbin Cai
Summary: This study designed a glycopeptide, Bio-Gluc-RGD, containing a cell adhesion motif-RGD and saccharide-glucose, to promote the formation of 3D spheroid structures of hiPSC-CMs. The hiPSC-CMs in spheroids showed phenotypic maturity and robust gap junctions through the activation of the integrin/ILK/p-AKT/Gata4 pathway. Encapsulated in the Bio-Gluc-RGD hydrogel, mono-dispersed hiPSC-CMs were more likely to form aggregates and survive in the infarcted myocardium, accompanied by enhanced gap junction formation and angiogenic effects. This study provides a novel strategy for hiPSC-CM maturation and potential application in heart regeneration after myocardial infarction.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Multidisciplinary Sciences
Karin H. Nilsson, Petra Henning, Maha El Shahawy, Maria Nethander, Thomas Levin Andersen, Charlotte Ejersted, Jianyao Wu, Karin L. Gustafsson, Antti Koskela, Juha Tuukkanen, Pedro P. C. Souza, Jan Tuckermann, Mattias Lorentzon, Linda Engstrom Ruud, Terho Lehtimaki, Jon H. Tobias, Sirui Zhou, Ulf H. Lerner, J. Brent Richards, Sofia Moverare-Skrtic, Claes Ohlsson
Summary: Genetic association signals at the RSPO3 locus are linked to fracture risk in humans. Studies show that the fracture-reducing allele at the RSPO3 locus leads to increased RSPO3 expression, reducing the risk of fractures.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Daishi Yamakawa, Daisuke Katoh, Kousuke Kasahara, Takashi Shiromizu, Makoto Matsuyama, Chise Matsuda, Yumi Maeno, Masatoshi Watanabe, Yuhei Nishimura, Masaki Inagaki
Summary: This study reveals that depletion of trichoplein, a key regulator of cilium formation, in mesenchymal progenitors results in longer cilia and inhibits adipocyte differentiation. Mechanistically, elongated cilia prevent the formation of lipid rafts around the ciliary base, affecting insulin signaling and adipogenesis. Knockout mice lacking trichoplein show reduced body fat and smaller adipocytes when fed a high-fat diet, indicating the crucial role of primary cilia in regulating adipogenic signal transduction.
Review
Cell Biology
Yuhei Nishimura, Yasunari Kanda, Hideko Sone, Hiroaki Aoyama
Summary: The developing brain is highly sensitive to chemicals, and exposure to neurotoxicants during the perinatal period is associated with various neurodevelopmental disorders. Studies have identified oxidative stress as a key event in many adverse outcome pathways related to developmental neurotoxicity.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Neurosciences
Naoki Iwasa, Takeshi K. Matsui, Naohiko Iguchi, Kaoru Kinugawa, Naritaka Morikawa, Yoshihiko M. Sakaguchi, Tomo Shiota, Shinko Kobashigawa, Mari Nakanishi, Masaya Matsubayashi, Riko Nagata, Sotaro Kikuchi, Tatsuhide Tanaka, Nobuyuki Eura, Takao Kiriyama, Tesseki Izumi, Kozue Saito, Hiroshi Kataoka, Yuichi Saito, Wataru Kimura, Akio Wanaka, Yuhei Nishimura, Eiichiro Mori, Kazuma Sugie
Summary: This study utilized cerebral organoids derived from human induced pluripotent stem cells to evaluate the impact of ischemic stroke on human cerebral tissue. Pathway analysis revealed significant relationships between various metabolic pathways and the complement and coagulation cascades. Key markers of neuronal cells in response to OGD/R were identified as the fatty acids-related PPAR signaling pathway and pyruvate kinase isoform M2 (PKM2).
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Pediatrics
Yuka Adachi, Aina Higuchi, Eri Wakai, Takashi Shiromizu, Junko Koiwa, Yuhei Nishimura
Summary: Palatogenesis is influenced by various factors, including gene polymorphisms and exposure to toxic chemicals. Studies have identified Mkx as a potential regulator in palatogenesis controlled by TGF beta and SHH signaling, suggesting its impairment could be linked to cleft palate etiology.
CONGENITAL ANOMALIES
(2022)
Review
Pharmacology & Pharmacy
Yuhei Nishimura, Kenji Kurosawa
Summary: This mini-review discusses the interaction between genetic and environmental factors in developmental disorders (DDs) using genome-wide and candidate gene-based approaches. It focuses on zebrafish and mouse models and introduces novel methods for analyzing G x E associated with DDs.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Munekazu Komada, Yuhei Nishimura
Summary: Neuroinflammation is linked to epigenetic dysregulation and may contribute to neurodevelopmental disorders. Understanding the role of epigenetics in microglia is crucial for studying neuroinflammation, and research using both mammals and zebrafish is valuable.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Physiology
Hironori Oshita, Hirofumi Sawada, Yoshihide Mitani, Naoki Tsuboya, Jane C. Kabwe, Junko Maruyama, Ali Yusuf, Hiromasa Ito, Ryuji Okamoto, Shoichiro Otsuki, Noriko Yodoya, Hiroyuki Ohashi, Kazunobu Oya, Yuhko Kobayashi, Issei Kobayashi, Kaoru Dohi, Yuhei Nishimura, Shinji Saitoh, Kazuo Maruyama, Masahiro Hirayama
Summary: Perinatal hypoxia may worsen occlusive pulmonary vascular disease in pulmonary arterial hypertension, leading to worsening hemodynamics and survival rate, as well as increased right ventricular hypertrophy and indicators of PVD. Perinatal hypoxia accelerates the proliferation and inflammation of pulmonary artery smooth muscle cells, which is associated with DNA methylation.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Eri Wakai, Kenji Ikemura, Toshiro Mizuno, Kazuhiko Takeuchi, Satoshi Tamaru, Masahiro Okuda, Yuhei Nishimura
Summary: The proton pump inhibitor lansoprazole (LPZ) has been found to reduce the ototoxicity caused by cisplatin (CDDP) by inhibiting the organic cation transporter OCT2. In vivo fluorescence imaging of zebrafish hair cells confirmed this hypothesis, and retrospective studies on human patients showed a significant decrease in ototoxicity incidence in those co-treated with LPZ. These findings suggest that LPZ may protect against CDDP-induced ototoxicity.
FRONTIERS IN PHARMACOLOGY
(2022)
Editorial Material
Pharmacology & Pharmacy
Letizia Polito, Wawaimuli Arozal, Yuhei Nishimura, Aprilita Rina Yanti Eff, Chiranjib Chakraborty
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Daishi Yamakawa, Junya Tsuboi, Kousuke Kasahara, Chise Matsuda, Yuhei Nishimura, Tatsuya Kodama, Naoyuki Katayama, Masatoshi Watanabe, Masaki Inagaki
Summary: After injury, FAPs play a key role in skeletal muscle regeneration and inhibition of adipogenesis through cilia-mediated signaling pathways. Dysfunction of cilia affects the insulin/Akt signaling pathway and impairs adipogenic differentiation of FAPs. Ciliary elongation promotes IL13 production in FAPs, facilitating myoblast proliferation and M2 macrophage polarization.
Editorial Material
Pharmacology & Pharmacy
Yuhei Nishimura, Tetsuhiro Kudoh, Munekazu Komada
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Masaki Saito, Wataru Otsu, Keiko Miyadera, Yuhei Nishimura
Summary: In this review, the potential of the primary cilium as a therapeutic target is examined, focusing on ciliary receptors, ciliogenesis, and ciliary trafficking. The mechanisms of selective distribution, signal transduction, and physiological roles of ciliary receptors are discussed, along with pathways that regulate ciliogenesis and the mechanism of ciliary trafficking. Current therapeutic advancements using large animal models of photoreceptor ciliopathy are also highlighted.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Endocrinology & Metabolism
Yusuf Ali, Elise P. Gomez-Sanchez, Celso E. Gomez-Sanchez
Summary: The mTOR signaling pathway plays a crucial role in transducing the AngII signal initiating aldosterone and cortisol synthesis in HAC15 cells.
Article
Respiratory System
Jane Chanda Kabwe, Hirofumi Sawada, Yoshihide Mitani, Hironori Oshita, Naoki Tsuboya, Erquan Zhang, Junko Maruyama, Yoshiki Miyasaka, Hideyoshi Ko, Kazunobu Oya, Hiromasa Ito, Noriko Yodoya, Shoichiro Otsuki, Hiroyuki Ohashi, Ryuji Okamoto, Kaoru Dohi, Yuhei Nishimura, Tomoji Mashimo, Masahiro Hirayama, Kazuo Maruyama
Summary: This study reveals that the Bmpr2 mutation in patients with pulmonary arterial hypertension exacerbates the clinical features of the disease and has significant effects on pulmonary vascular disease and survival rates in a rat model of pulmonary hypertension.
RESPIRATORY RESEARCH
(2022)