Article
Multidisciplinary Sciences
Manash Jyoti Kalita, Kalpajit Dutta, Gautam Hazarika, Ridip Dutta, Simanta Kalita, Partha Pratim Das, Manash P. Sarma, Sofia Banu, Md. Ghaznavi Idris, Anjan Jyoti Talukdar, Sangitanjan Dutta, Ajanta Sharma, Subhash Medhi
Summary: As the COVID-19 pandemic continues to impact the world, the development of a cost-effective and efficient multiplex PCR detection assay for the virus has shown promising results. The study demonstrated rapid and sensitive detection of SARS-CoV-2 using innovative PCR techniques, with results comparable to commercially available kits. The novel approach has the potential for extended commercial applicability and offers a practical solution for resource-limited settings.
SCIENTIFIC REPORTS
(2021)
Review
Medical Laboratory Technology
Jie Guo, Jiaxin Ge, Yanan Guo
Summary: Effective methods for diagnosing COVID-19 include qRT-PCR, RNA sequencing, serological testing, and imaging technologies. Varying healthcare systems around the world lead to different diagnostic tools being used, with artificial intelligence improving efficiency in imaging-based methods.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Virology
Selin Unsaler, Ayse Okan, Suda Tekin, Aysenur M. Hafiz, Ozan Gokler, Ozan Altuntas
Summary: The study aimed to compare RT-PCR results of NA and NS samples in the diagnosis of COVID-19. The results showed no statistical difference in Ct values between NA and NS samples, indicating similar effectiveness of the two sampling methods in detecting the coronavirus.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Selen Zeliha Mart Komurcu, Yakup Artik, Nevra Pelin Cesur, Arzu Tanriverdi, Derya Cakir Erdogan, Sule Celik, Elif Yilmaz Gulec
Summary: Rapid and reliable detection of SARS-CoV-2 mutations is crucial in controlling virus transmission. Study found N501Y mutation in positive patients, providing important information for public health efforts.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Infectious Diseases
Abhinav Jain, Mercy Rophina, Saurabh Mahajan, Bhavya Balaji Krishnan, Manasa Sharma, Sreya Mandal, Teresa Fernandez, Sumayra Sultanji, Bani Jolly, Samatha Mathew, Sridhar Sivasubbu, Vinod Scaria
Summary: The SARS-CoV-2 epidemic originating from Wuhan rapidly evolved into a global pandemic, highlighting the importance of molecular detection using RT-PCR, although mutations in the viral genome could impact the accuracy of detection assays. The analysis of global SARS-CoV-2 genomes identified multiple genetic variants that could affect the effectiveness of diagnostic primers and probes.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Virology
George M. Bwire, Mtebe Majigo, Belinda J. Njiro, Akili Mawazo
Summary: Testing using appropriate specimens and accurate diagnostic tests like qRT-PCR is important for curbing the spread of COVID-19. A systematic review found high detection rates in lower respiratory tract specimens, while no virus was detected in urinogenital specimens. Bronchoalveolar lavage had the highest positive rate followed by rectal swabs and sputum. Nasopharyngeal swabs, commonly used, had a moderate positive rate. Low rates were observed in oropharyngeal swabs and blood samples, while no virus was found in urine samples. Fecal samples showed a positive rate, suggesting potential fecal transmission of SARS-CoV-2.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Virology
Jonghoo Lee, Jae-Uk Song
Summary: The Xpert Xpress assay demonstrated excellent diagnostic accuracy for rapid detection of SARS-CoV-2, while the ID NOW assay showed relatively low sensitivity, potentially missing some positive samples.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Infectious Diseases
Daisuke Tamura, Hirokazu Yamagishi, Yuji Morisawa, Takashi Mato, Shin Nunomiya, Yuta Maehara, Yasushi Ochiai, Shinya Okuyama, Narumi Ohmika, Takanori Yamagata, Hitoshi Osaka
Summary: This study aimed to validate a newly developed antigen-detecting rapid diagnostic test (Ag-RDT) for SARS-CoV-2 using anterior nasal specimens. The results showed that the Ag-RDT had high sensitivity in the early stage of the disease and remained specific at 100%. Therefore, anterior nasal specimens can be considered as a reliable sample source.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Environmental Sciences
Vhahangwele Masindi, Spyros Foteinis, Kefilwe Nduli, Vhahangwele Akinwekomi
Summary: Human coronaviruses, including SARS and MERS, have caused global concern with high mortality rates. The COVID-19 pandemic caused by SARS-CoV-2 virus is currently unprecedented. Studies in a South African setting show that SARS-CoV-2 viral RNA decays in wastewater treatment stages, suggesting low likelihood of transmission in water catchments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biophysics
John Daniels, Shekhar Wadekar, Ken DeCubellis, George W. Jackson, Alexander S. Chiu, Quentin Pagneux, Hiba Saada, Ilka Engelmann, Judith Ogiez, Delphine Loze-Warot, Rabah Boukherroub, Sabine Szunerits
Summary: This study introduces a novel concept of using exhaled breath condensate (EBC) collected by a mask-based sampling device and detected with an electrochemical biosensor for fast and specific detection of COVID-19. Results showed detection of SARS-CoV-2 in samples from positive patients, and the electrochemical biosensor was able to detect viral particles, providing information about the infectivity state of the patients.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Public, Environmental & Occupational Health
B. Dinc, F. Kirca, S. Aydogan, A. Toyran, T. Basyigit, I Omay, P. Gun, M. Caglayan, A. A. Surel
Summary: This retrospective study analysed SARS-CoV-2 Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) test results from a pandemic hospital in Turkey, finding higher positivity among females and younger patients (0-18 years old), as well as significantly higher positivity in August and September. This could contribute to the lower COVID-19 mortality rates in Turkey.
Article
Chemistry, Multidisciplinary
Soo Khim Chan, Pinyi Du, Caroline Ignacio, Sanjay Mehta, Isabel G. Newton, Nicole F. Steinmetz
Summary: COVID-19 is a highly transmissible disease affecting majority of countries globally. The use of nucleic acid tests, especially RT-PCR, has become essential for early detection of infection. Generating biomimetic virus-like particles as positive controls can overcome the need for cold-chain distribution, providing reliable, scalable, and stable controls for clinical settings.
Article
Infectious Diseases
Ming- Jian, Cherng-Lih Perng, Hsing-Yi Chung, Chih-Kai Chang, Jung-Chung Lin, Kuo-Ming Yeh, Chien-Wen Chen, Shan-Shan Hsieh, Pin-Ching Pan, Hao-Ting Chang, Feng-Yee Chang, Ching-Liang Ho, Hung-Sheng Shang
Summary: This study evaluated the performance of the COVID-19 antigen rapid test kit in a community testing site. The results showed high sensitivity and specificity of the test, especially in subjects with cycle threshold values <= 24. The test also effectively detected the SARS-CoV-2 B.1.1.7 lineage.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Virology
Carolin Ade, Joachim Pum, Iris Abele, Lubna Raggub, Dirk Bockmuhl, Bernhard Zollner
Summary: The study aimed to monitor Ct values of S gene SARS-CoV-2 in patients and found that females were older than males with slightly higher Ct values, patients over 80 had significantly higher Ct values, and children showed Ct values similar to adults. Additionally, age was an important factor in Ct values, with inpatients testing positive for SARS-CoV-2 being significantly older than outpatients.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Virology
Caroline Lefeuvre, Adeline Pivert, Emilie Przyrowski, Elise Bouthry, Estelle Darviot, Rafael Mahieu, Francoise Lunel-Fabiani, Alexandra Ducancelle, Helene Le Guillou-Guillemette
Summary: The study compares the performance of three molecular SARS-CoV-2 assays and finds that the Hologic assays exhibit higher accuracy and faster turnaround time, making them suitable for high prevalence periods.
JOURNAL OF MEDICAL VIROLOGY
(2022)