Review
Cell & Tissue Engineering
Ali Rajool Dezfuly, Azadeh Safaee, Hossein Salehi
Summary: Extracellular vesicles (EVs), including microvesicles and exosomes, carry vital information such as lipids, proteins, mRNAs, and microRNAs to target cells. Studies have shown that the therapeutic effects of stem cells are attributed to the miRNAs in their EVs, which may help in developing new therapeutic techniques for retinal degenerative diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Jing Wang, Wanru Liu, Dehai Yu, Zongxing Yang, Sijie Li, Xiguang Sun
Summary: Premature ovarian failure is a major cause of infertility in women of childbearing age. Hormone replacement therapy is commonly used to alleviate symptoms, but stem cell transplantation has emerged as a promising research area for restoring ovarian function and fertility in affected patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Notsile H. Dlamini, Tina Nguyen, Ahmed Gad, Dawit Tesfaye, Shengfa F. Liao, Scott T. Willard, Peter L. Ryan, Jean M. Feugang
Summary: Sperm heterogeneity presents challenges for artificial insemination success. MicroRNAs (miRNAs) isolated from seminal plasma-derived extracellular vesicles (SP-EV) of boars have potential as non-invasive biomarkers for sperm quality. In this study, SP-EVs were isolated and characterized, and miRNAs were identified and analyzed. Fifteen differentially expressed miRNAs were found between poor- and good-quality sperm, with three targeting genes associated with cellular localization and molecular functions. PTEN and YWHAZ were identified as essential proteins for protein kinase binding. The findings suggest that SP-EV-derived miRNAs can serve as indicators of boar sperm quality and contribute to therapeutic strategies for fertility improvement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hiroshi Maruoka, Tomohito Tanaka, Hikaru Murakami, Hiromitsu Tsuchihashi, Akihiko Toji, Misa Nunode, Atsushi Daimon, Shunsuke Miyamoto, Ruri Nishie, Shoko Ueda, Sousuke Hashida, Shinichi Terada, Hiromi Konishi, Yuhei Kogata, Kohei Taniguchi, Kazumasa Komura, Masahide Ohmichi
Summary: This study identified cancer-specific miRNAs in tissue-exudative extracellular vesicles (Te-EVs) of ovarian clear cell carcinomas (OCCs). The levels of these miRNAs were significantly decreased in postoperative serum samples, indicating their potential as biomarkers for the early diagnosis and detection of OCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Isabella Fabietti, Tiago Nardi, Chiara Favero, Laura Dioni, Laura Cantone, Laura Pergoli, Mirjam Hoxha, Eva Pinatel, Fabio Mosca, Valentina Bollati, Nicola Persico
Summary: In infants with congenital diaphragmatic hernia (CDH) undergoing FETO, survivors had lower counts of EVs in amniotic fluid while non-survivors had higher counts of EVs in both amniotic and tracheal fluid. Differential regulation of certain EV-enclosed miRNAs was associated with postnatal survival.
Review
Biochemistry & Molecular Biology
Yasunari Matsuzaka, Ryu Yashiro
Summary: This review summarizes the advances in the purification, modification, and application of extracellular vesicles as a novel strategy for treating certain diseases.
Article
Cell Biology
Chun-Chieh Huang, Miya Kang, Kasey Leung, Yu Lu, Sajjad Shirazi, Praveen Gajendrareddy, Sriram Ravindran
Summary: Mesenchymal stem cell derived extracellular vesicles (MSC EVs) possess immunomodulatory and therapeutic properties. This study demonstrates that engineering MSC EVs with increased levels of miR-424 enhances their osteoinductive function and facilitates bone repair in vitro and in vivo. The engineered EVs retain their characteristics and immunomodulatory properties, providing proof-of-concept for miRNA-based extracellular vesicles engineering in regenerative medicine applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Endocrinology & Metabolism
Congcong Guo, Yubo Ma, Yanqiu Situ, Li Liu, Guoqun Luo, Huan Li, Wenmin Ma, Li Sun, Wen Wang, Qiuying Weng, Linlin Wu, Dazhi Fan
Summary: This study conducted a systematic review and meta-analysis and found that the transplantation of mesenchymal stem cells (MSCs) may have therapeutic effects on animal models of premature ovarian failure (POF), potentially by improving sexual cycle disorder, modulating serum hormone expressions, and restoring ovarian function.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Gastroenterology & Hepatology
Qichen Shen, Zhuizui Huang, Lingyan Ma, Jiachen Yao, Ting Luo, Yao Zhao, Yingping Xiao, Yuanxiang Jin
Summary: Inflammatory bowel disease (IBD) is a global disease without a cure, and disruption of the microbial ecosystem is considered a major cause. This study investigated the role of extracellular vesicles (EVs) and membrane vesicles (MVs) in host-microbe interactions in colitis. The researchers found that miR-181b-5p transplantation inhibited inflammation and promoted macrophage polarization, while miR-200b-3p regulated the microbiota composition and contributed to intestinal barrier integrity. MVs from feces and BMVs from colitis also had similar effects. These findings provide new insights and potential treatment strategies for colitis.
Article
Reproductive Biology
Osama G. Sakr, Ahmed Gad, Karina Canon-Beltran, Yulia N. Cajas, Radek Prochazka, Dimitrios Rizos, Pilar G. Rebollar
Summary: This study aimed to isolate, characterize, and identify the miRNA expression profiles in the seminal plasma extracellular vesicles (SP-EVs) isolated from fertile and subfertile rabbit bucks. The concentration of SP-EVs was positively correlated with abnormal forms and immotile spermatozoa. Small-RNA-seq analysis identified several miRNAs that were significantly up- and down-regulated in the fertile compared to the subfertile group. These findings suggest that SP-EVs with different miRNA cargo could serve as biomarkers for male fertility and potential therapies for assisted reproductive technologies.
Review
Cell Biology
Zixiang Geng, Hailing Guo, Yifei Li, Ying Liu, Yongfang Zhao
Summary: Primary ovarian insufficiency (POI) is a major cause of female fertility loss in young women. Stem cell transplantation is a potential intervention for treating POI, but its clinical application is limited due to issues such as tumorigenesis and ethical debates. Stem cell-derived extracellular vesicles have shown exciting therapeutic effects for POI by improving ovarian reserve, follicle growth, and hormone levels.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Jing Zhang, Juan Gao, Xianlong Li, Dengna Lin, Zhihui Li, Jialei Wang, Junfeng Chen, Zhiliang Gao, Bingliang Lin
Summary: The efficacy of human bone marrow MSC-derived sEVs in treating ALF was investigated, and the molecular mechanisms involved in regulating hepatocyte proliferation and apoptosis were explored. The results showed that BMSC-sEVs improved survival rates and reduced liver injury in ALF mice. This effect was achieved by upregulating miR-20a-5p, which targeted the PTEN/AKT signaling pathway to reduce hepatocyte apoptosis and promote cell proliferation.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Cell Biology
Ekaterina Zubkova, Evgeniy Evtushenko, Irina Beloglazova, German Osmak, Phillip Koshkin, Alexander Moschenko, Mikhail Menshikov, Yelena Parfyonova
Summary: Research has shown that rat adipose-derived MSC-overexpressing stem cell factor (SCF) can induce endogenous regenerative processes and improve cardiac function. Genetic modification of MSC has a great impact on their miRNA composition. This study provides new insights into the regulatory networks underlying extracellular vesicle effects.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Immunology
Claire Loussouarn, Yves-Marie Pers, Claire Bony, Christian Jorgensen, Daniele Noel
Summary: MSCs stimulate tissue repair by releasing EVs, which contain various molecules including miRNAs. These EVs impact the metabolism and oxidative stress of target cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Steven Kopcho, Marina McDew-White, Wasifa Naushad, Mahesh Mohan, Chioma M. Okeoma
Summary: This study comprehensively analyzed the composition of circulating exmiRNAs and their association with extracellular particles, such as EVs and ECs, using sRNA sequencing. The study also found that SIV infection can alter the miRNA profile of EVs and suggested that miR-128-3p may be a potential target of HIV/SIV. This research has important implications for the development of biomarker approaches for various diseases based on the analysis of circulating exmiRNAs.
Review
Cell & Tissue Engineering
Radwa A. Mehanna, Marwa M. Essawy, Mona A. Barkat, Ashraf K. Awaad, Eman H. Thabet, Heba A. Hamed, Hagar Elkafrawy, Nehal A. Khalil, Abeer Sallam, Marwa A. Kholief, Samar S. Ibrahim, Ghada M. Mourad
Summary: Regenerative medicine focuses on repairing and restoring damaged human tissues and organs. Stem cells, particularly cardiac stem and progenitor cells (CSCs/CPCs), have shown promise in enhancing the regenerative capacity of the heart. This review explores the nature of CSCs/CPCs, their interactions with other cells, and their potential clinical applications in cardiac regeneration. Additionally, the role of nanotechnology in this field is discussed.
WORLD JOURNAL OF STEM CELLS
(2022)
Article
Pharmacology & Pharmacy
Salma El-Sayed Radwan, Riham M. El-Moslemany, Radwa A. Mehanna, Eman H. Thabet, Elsayeda-Zeinab A. Abdelfattah, Amal El-Kamel
Summary: This study developed chitosan-coated bovine serum albumin nanoparticles (CS-BSA-NPs) for the delivery of tetrandrine (TET) in the management of glaucoma. The CS-BSA-NPs showed enhanced corneal permeation and biocompatibility, as well as improved cellular uptake, antioxidant, and anti-proliferative properties compared to uncoated nanoparticles.
Article
Pharmacology & Pharmacy
Sahar A. Harby, Nehal A. Khalil, Norhan S. El-Sayed, Eman H. Thabet, Samar R. Saleh, Mona Hassan Fathelbab
Summary: The overexpression of breast cancer resistance protein (BCRP), a blood-brain barrier efflux transporter, hampers the entry of antiepileptic drugs into the brain. Inhibition of BCRP improves the bioavailability and efficacy of lamotrigine, playing an important role in the management of drug-resistant epilepsy.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)