Article
Cell Biology
Petra Vesela, Jakub Zahumensky, Jan Malinsky
Summary: Eisosomes are large structures found in the plasma membrane of various fungi, lichens, and unicellular algae. They are organized by Pil1 proteins and under certain stress conditions, Lsp1 can substitute for the Pil1 function in eisosome formation. Other proteins, such as Seg1 and Nce102, are also required for eisosome assembly. Our study revealed that the inability to form eisosomes affects the expression of genes involved in oxidative phosphorylation, tricarboxylic acid cycle, DNA repair, ribosome biogenesis, and cell cycle.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Amita Pal, Arun Kumar Paripati, Pallavi Deolal, Arpan Chatterjee, Pushpa Rani Prasad, Priyanka Adla, Naresh Babu V. Sepuri
Summary: Mitochondrial morphology and dynamics play crucial roles in maintaining mitochondrial integrity and function. This study identifies Pil1, a major component of the eisosome complex, as a regulator of mitochondrial morphology, mitophagy, and cell death.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Carla E. Lanze, Sai Zhou, James B. Konopka
Summary: The study demonstrated that the N-terminal region of Sur7 is responsible for its localization to MCC/eisosomes, while the C-terminal domain is crucial for resisting cell wall stress. Additionally, Sur7 plays an important role in the cytoplasm.
MOLECULAR MICROBIOLOGY
(2021)
Article
Cell Biology
Katherine M. Paine, Kamilla M. E. Laidlaw, Gareth J. O. Evans, Chris MacDonald
Summary: The plasma membrane of yeast is organized into specific subdomains that regulate surface membrane proteins. Transporters actively uptake nutrients in particular regions of the plasma membrane where they are also protected from endocytosis. The core eisosome subunit Pil1, required for eisosome biogenesis, is phosphorylated primarily by the kinase Pkh2 and is rapidly dephosphorylated in response to glucose starvation. Precise post-translational control of Pil1 modulates nutrient transporter retention within eisosomes, depending on extracellular nutrient levels, to maximize recovery following starvation.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ken-taro Sakata, Keisuke Hashii, Koushiro Yoshizawa, Yuhei O. Tahara, Kaori Yae, Ryohei Tsuda, Naotaka Tanaka, Tatsuya Maeda, Makoto Miyata, Mitsuaki Tabuchi
Summary: This study investigates the coordinated function of MCC proteins in maintaining MCC function and stress response. The results reveal that the Sur7 family and Nce102 family cooperate with Pil1 protein in stress tolerance and eisosome assembly. Furthermore, Pil1 and 6-Tsp proteins also regulate TORC2 signaling cooperatively.
MOLECULAR MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Julia Bahja, Marcus K. Dymond
Summary: Oxidative stress affects lipid membranes by reducing stored curvature elastic energy, which can potentially be mitigated by compounds that increase stored curvature elastic energy; Age-related changes in lipid composition may represent a homeostatic response of cells to compensate for the accumulation of in vivo lipid oxidation products.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Editorial Material
Cell Biology
Shree Padma Metur, Daniel J. Klionsky
Summary: This study reconstitutes the initial steps of autophagosome biogenesis during selective autophagy using autophagy factors purified from yeast, demonstrating that Atg9 vesicles play crucial roles in recruiting the autophagy machinery and establishing membrane contact sites for lipid transfer during autophagosome biogenesis.
Article
Agriculture, Multidisciplinary
Xiaofan Jin, Huirong Yang, Teodora Emilia Coldea, Mogens Larsen Andersen, Haifeng Zhao
Summary: The study found that wheat gluten peptides have positive effects on cell growth, membrane lipid composition, and membrane homeostasis of yeast. Supplementation of wheat gluten peptides enhances the ethanol stress tolerance of yeast.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Cell Biology
Mamata Malla, Debatrayee Sinha, Pritha Chowdhury, Benjamin Thomas Bisesi, Qian Chen
Summary: This study analyzed the structure-function relationship of Pkd2 and found that the extracellular lipid-binding and transmembrane domains are crucial for its targeting to the plasma membrane, while the cytoplasmic domain ensures its polarized distribution. The study revealed the importance of Pkd2's intracellular trafficking and function.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Anete Romanauska, Alwin Koehler
Summary: This study reveals the essential role of lipid acyl chain unsaturation in the structure and function of the nuclear envelope and nuclear pore complex. Balanced lipid saturation is required for the integrity of the nuclear pore complex and nucleocytoplasmic transport.
NATURE CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Leonie Lehmayer, Lukas Bernauer, Anita Emmerstorfer-Augustin
Summary: The study successfully established the AID system for Komagataella phaffii yeast, which allows rapid depletion of target proteins. The research also revealed the essential role of Wsc-type sensors in cell growth, and demonstrated the usefulness of the AID system for gene analysis in K. phaffii.
Article
Biochemistry & Molecular Biology
Broderick L. Bills, Michelle K. Knowles
Summary: Phosphatidic acid (PA), a signaling lipid with a negative charge and small headgroup, is capable of localizing to regions of negative curvature and supporting the formation of curved, tubulated membranes.
Article
Multidisciplinary Sciences
Raviv Dharan, Shahar Goren, Sudheer Kumar Cheppali, Petr Shendrik, Guy Brand, Alisa Vaknin, Li Yu, Michael M. Kozlov, Raya Sorkin
Summary: This study uncovers the mechanisms by which tetraspanin4 and CD9, two tetraspanin proteins, prefer positive membrane curvature and play important roles in migrasome formation and oocyte microvilli shaping. These findings shed light on the membrane remodeling processes and provide insights into the functions of tetraspanin proteins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agriculture, Multidisciplinary
Caiyun Wu, Li Liu, Mengmeng Zhang, Xiaolan Jike, Hexin Zhang, Nana Yang, Huirong Yang, Huaide Xu, Hongjie Lei
Summary: High concentrations of ethanol can cause oxidative stress in yeast, leading to ethanol-oxidation cross-stress. Antioxidant dipeptides help maintain cell viability and stress tolerance. This study investigated how antioxidant dipeptides affect the yeast cell wall and membrane defense systems to enhance stress tolerance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Cell Biology
Md. Istiaq Obaidi Tanvir, Go Itoh, Hiroyuki Adachi, Shigehiko Yumura
Summary: The study observed wound repair in dividing cells for the first time and found dynamic changes of myosin II during cell membrane repair. It was discovered that myosin II is not essential for cell wound repair, and a novel mechanism for its delocalization from the cortex through phosphorylation was proposed. Additionally, the disappearance of several furrow-localizing proteins upon wounding was observed, indicating potential mechanisms for myosin dynamics during wound repair.
Article
Biochemistry & Molecular Biology
Salvatore M. Parrino, Haoyu Si, Shamoon Naseem, Kevin Groudan, Justin Gardin, James B. Konopka
MOLECULAR MICROBIOLOGY
(2017)
Article
Genetics & Heredity
Lois M. Douglas, James B. Konopka
Article
Microbiology
Kyunghun Min, Amy Biermann, Deborah A. Hogan, James B. Konopka
Article
Infectious Diseases
Rafael M. Gandra, Laura N. Silva, Xenia M. Souto, Leandro S. Sangenito, Lucas P. S. Cruz, Lys A. Braga-Silva, Diego S. Goncalves, Sergio H. Seabra, Marta H. Branquinha, Andre L. S. Santos
Article
Microbiology
Rafael M. Gandra, Pauraic McCarron, Livia Viganor, Mariana Farias Fernandes, Kevin Kavanagh, Malachy McCann, Marta H. Branquinha, Andre L. S. Santos, Orla Howe, Michael Devereux
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Immunology
Jenna E. Foderaro, James B. Konopka
Summary: The survival of the fungal pathogen Candida albicans in a mammalian host relies on its ability to resist oxidative stress through flavodoxin-like proteins. Among these proteins, Pst3 plays a major role in promoting resistance to oxidative stress induced by small quinone molecules. Analysis of protein levels and induction with p-benzoquinone showed Pst1 and Pst3 to be the most abundant and protective against oxidative damage, demonstrating the distinct roles of flavodoxin-like proteins in resisting oxidative stress.
INFECTION AND IMMUNITY
(2021)
Article
Microbiology
Leo D. Bemena, Kyunghun Min, James B. Konopka, Aaron M. Neiman
Summary: Chitosan plays a crucial role in stress resistance and virulence in fungal species. The formation of a chitosan layer in the cell walls of different yeast species requires a conserved set of genes, suggesting potential new targets for antifungal therapies aimed at disrupting cell wall function.
Article
Biochemistry & Molecular Biology
Carla E. Lanze, Sai Zhou, James B. Konopka
Summary: The study demonstrated that the N-terminal region of Sur7 is responsible for its localization to MCC/eisosomes, while the C-terminal domain is crucial for resisting cell wall stress. Additionally, Sur7 plays an important role in the cytoplasm.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Kyunghun Min, Thomas F. Jannace, Haoyu Si, Krishna R. Veeramah, John D. Haley, James B. Konopka
Summary: The fungal pathogen Candida albicans can form hyphae in the absence of cAMP, due to mutations that improve growth through loss of the negative regulatory subunit BCY1. Deletion of large regions of chromosome 2's left arm can enhance hyphal morphogenesis in some mutants. Gene expression related to hyphal formation can be induced independently of cAMP.
Review
Microbiology
Kyunghun Min, Shamoon Naseem, James B. Konopka
Review
Microbiology
Jenna E. Foderaro, Lois M. Douglas, James B. Konopka
Article
Plant Sciences
Marina Nadal, Ruairidh Sawers, Shamoon Naseem, Barbara Bassin, Corinna Kulicke, Abigail Sharman, Gynheung An, Kyungsook An, Kevin R. Ahern, Amanda Romag, Thomas P. Brutnell, Caroline Gutjahr, Niko Geldner, Christophe Roux, Enrico Martinoia, James B. Konopka, Uta Paszkowski