期刊
MATRIX BIOLOGY
卷 95, 期 -, 页码 1-14出版社
ELSEVIER
DOI: 10.1016/j.matbio.2020.10.001
关键词
Decorin; Tumor microenvironment; EMT; AOM/DS; Colorectal cancer; Celecoxib
资金
- National Nature Science foundation of China [81472821]
- LiaoNing Revitalization Talents Program [XLYC 1807058]
- National Institutes of Health [CA39481]
- Key Grant of Education department of Liaoning Province [LZD201904]
The study reveals the significant role of a decorin-deficient tumor microenvironment in colon carcinoma progression and metastasis, with promising therapeutic efficacy demonstrated in decorin-deficient animals through a combined Celecoxib/decorin treatment.
The tumor microenvironment encompasses a complex cellular network that includes cancer-associated fibroblasts, inflammatory cells, neo-vessels, and an extracellular matrix enriched in angiogenic growth factors. Decorin is one of the main components of the tumor stroma, but it is not expressed by cancer cells. Lack of this proteoglycan correlates with down-regulation of E-cadherin and induction of beta-catenin signaling. In this study, we investigated the role of a decorin-deficient tumor microenvironment in colon carcinoma progression and metastasis. We utilized an established model of colitis-associated cancer by administering Azoxymethane/Dextran sodium sulfate to adult wild-type and Dcn(-/-) mice. We discovered that after 12 weeks, all the animals developed intestinal tumors independently of their genotype. However, the number of intestinal neoplasms was significantly higher in the Dcn(-/-) microenvironment vis-a-vis wild-type mice. Mechanistically, we found that under unchallenged basal conditions, the intestinal epithelium of the Dcn(-/-) mice showed a significant increase in the protein levels of epithelial-mesenchymal transition associated factors including Snail, Slug, Twist, and MMP2. In comparison, in the colitis-associated cancer evoked in the Dcn(-/-) mice, we found that intercellular adhesion molecule 1 (ICAM-1) was also significantly increased, in parallel with epithelial-mesenchymal transition signaling pathway-related factors. Furthermore, a combined Celecoxib/decorin treatment revealed a promising therapeutic efficacy in treating human colorectal cancer cells, in decorin-deficient animals. Collectively, our results shed light on colorectal cancer progression and provide a protein-based therapy, i.e., treatment using recombinant decorin, to target the tumor microenvironment. (C) 2020 Elsevier B.V. All rights reserved.
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