期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2020.111064
关键词
Fucoidan; Cell penetrating peptide; Nanoparticles; Targeting; Drug delivery; Imaging
资金
- Ministry of Science and Technology of Taiwan [MOST 102-2221-E-238-002-MY2, MOST 105-2314-B-038-016-]
P-selectin overexpressed on activated endothelial cells and platelets is a new target for treatment of cancers and cardiovascular diseases such as atherosclerosis and thrombosis. In this study, depolymerized low molecular weight fucoidan (LMWF8775) and a thermolysin-hydrolyzed prolamine peptide (TPP1880) were prepared. TPP1880 and LMWF8775 were able to form self-assembled complex nanoparticles (CNP5). The formation of TPP1880/LMWF8775 CNP5 was characterized by Fourier-transform infrared spectra, circular dichroism spectra and isothermal titration calorimetry. The CNP5 selectively targeted PMA-stimulated, inflamed endothelial cells (HUVECs) with high expression of P-selectin. Gd-DTPA MRI contrast agent was successfully loaded in the CNP5 with better T-1 relaxivity and selectively accumulated in the activated HUVECs with increased MRI intensity and reduced cytotoxicity as compared to free Gd-DTPA. Our results suggest that the TPP1880/LMWF8775 CNP5 may have potential in future for early diagnosis of cardiovascular diseases and cancers in which the endothelium is inflamed or activated.
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