期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2020.111587
关键词
Shark collagen; Chondrogenic differentiation; Cartilage regeneration; Marine biomaterials; Fish by-products
资金
- Portuguese Foundation for Science and Technology (FCT) [M-ERA-NET2/0022/2016]
- European Union through INTERREG Atlantic Programme [EAPA_151/2016, NORTE-08-5369-FSE-000037]
- Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Social Fund
- FCT [IF/00347/2015]
- Fundação para a Ciência e a Tecnologia [M-ERA-NET2/0022/2016] Funding Source: FCT
The potential of blue shark skin collagen to induce chondrogenic differentiation of human adipose stem cells was investigated, with hyaluronic acid playing a crucial role at later time points. The study suggests that PG collagen can support chondrogenic differentiation at early time points, but exogenous stimulation is required for phenotype maintenance.
Representing a strategy of marine by-products valorization, based on isolation of biocompounds and assessment of biomedical applicability, the potential of blue shark (Prionace glauca (PG)) skin collagen to induce chondrogenic differentiation of human adipose stem cells (hASC) was investigated, with and without exogenous stimulation. For that, a cryogelation method was applied to produce highly interconnected porous 3-dimensional (3D) constructs made of collagen and collagen:hyaluronic acid (20:1). In vitro studies reveal that hASC adhere abundantly to the constructs which then suggests the early chondrogenic differentiation of those cells. These findings are supported by the mRNA expression encoding chondrogenic-related markers like Coll II and Sox-9 that are markedly upregulated at an early stage for both conditions, with and without exogenous stimulation. The introduction of hyaluronic acid (Hya) seems to play a crucial role at later time points, as shown by the evident immunodetection of aggrecan (ACAN), even without exogenous stimulation. It is hypothesized that the PG collagen itself can support chondrogenic differentiation at early time points, but exogenous stimulation is required to ensure phenotype maintenance. The present work highlights the relevance of using blue shark collagen biopolymer as a building block to produce highly effective temporary matrices for cartilage applications.
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