Review
Infectious Diseases
Hiroki Namikawa, Ken-Ichi Oinuma, Koichi Yamada, Yukihiro Kaneko, Hiroshi Kakeya, Taichi Shuto
Summary: This systematic review and meta-analysis suggests that hypermucoviscous Klebsiella pneumoniae strains are associated with an increased risk of abscess formation in bacteremia cases, but not with a significant increase in mortality rate. The string test is reaffirmed as an important tool for detecting hypermucoviscous K. pneumoniae.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Microbiology
Deyan Donchev, Ivan N. N. Ivanov, Ivan Stoikov, Stefana Sabtcheva, Yordan Kalchev, Marianna Murdjeva, Elina Dobreva, Rumyana Hristova
Summary: The study improved and extended the multi-locus VNTR analysis (MLVA) for strain-level differentiation of Klebsiella pneumoniae species complex. The modified scheme (MLVA8+) showed high concordance with classic MLST and provided greater differentiation capability. MLVA8+ also demonstrated its applicability in investigating the intra-hospital spread of carbapenem-resistant producers. It offers improved typing accuracy and extended typing scope for Klebsiella pneumoniae.
Article
Public, Environmental & Occupational Health
C. Legouge, P. Bidet, M. Gits-Muselli, A. Cointe, C. Courroux, A. Birgy, S. Bonacorsi
Summary: This study evaluated a simple and rapid method of multi-locus variable number tandem repeat analysis (MLVA) as a screening tool to determine the relatedness of Klebsiella pneumoniae strains. The results showed that MLVA had a high discriminatory power and could be used for epidemiological investigation.
JOURNAL OF HOSPITAL INFECTION
(2023)
Article
Agriculture, Dairy & Animal Science
Tianle Xu, Xinyue Wu, Hainan Cao, Tianxu Pei, Yu Zhou, Yi Yang, Zhangping Yang
Summary: This study investigated the distribution of virulence gene expression and antimicrobial susceptibility in Klebsiella pneumoniae isolated from dairy cows with clinical mastitis in Jiangsu Province. The study revealed potential links between virulence gene expression and antimicrobial resistance. The results provide important insights into the spread of infection by K. pneumoniae and resistance in the case of bovine mastitis, as well as potential target genes involved in the links between virulence determinants and multidrug resistance.
Review
Microbiology
Jie Zhu, Tao Wang, Liang Chen, Hong Du
Summary: The review summarizes the virulence factors associated with hypervirulent Klebsiella pneumoniae to understand its molecular pathogenesis and provide new strategies for improving the prevention, diagnosis, and treatment of infections caused by hvKP.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jiao Liu, Zhuofei Xu, Haibo Li, Fuhui Chen, Kaiyu Han, Xiaoman Hu, Yuan Fang, Dechang Chen
Summary: In this study, metagenomic next-generation sequencing was used to analyze the genome biology of Klebsiella pneumoniae prevalent in China. Strain-level profiling and analyses of antimicrobial resistance and virulence genes were performed. The study highlights the advantages of strain-level metagenomics in understanding the resistance and virulence mechanisms of K. pneumoniae directly from clinical specimens.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Aamir Jamal Gondal, Sidrah Saleem, Nakhshab Choudhry, Fridoon Jawad Ahmad, Hina Bukhari, Nighat Yasmin, Shah Jahan
Summary: Carbapenemases are responsible for the spread of multidrug-resistant K. pneumoniae, and this study identified 78 carbapenem-resistant strains in Pakistan. The emergence of blaKPC-2 producing K. pneumoniae coharboring blaCTX-M and blaSHV in Pakistan is reported for the first time. This highlights the need for vigilant monitoring of high-risk clones to control the global spread of drug-resistant bacteria.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2023)
Article
Microbiology
Mi Nguyen-Tra Le, Thao Huu-Huong Nguyen, Van Minh Trinh, Tam Phuc-Bao Nguyen, Miki Kawada-Matsuo, Shizuo Kayama, Motoyuki Sugai, Hitoshi Komatsuzawa
Summary: This study identified bacteriocin genes in 180 K. pneumoniae species complex genomes and found that 32.8% of isolates carried at least one bacteriocin type. Microcin E492 was the most prevalent bacteriocin with a wide spectrum of antibacterial activity. Other bacteriocins had lower detection rates and limited inhibitory activity. These findings are important for understanding the K. pneumoniae species complex and the potential applications of bacteriocins in treating multidrug-resistant bacteria.
MICROBIOLOGY SPECTRUM
(2023)
Article
Public, Environmental & Occupational Health
Tian Wei, Chengyun Zou, Jie Qin, Jianmin Tao, Li Yan, Jiangjun Wang, Hong Du, Fang Shen, Yanqin Zhao, Haiying Wang
Summary: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) infections in older patients present clinical and molecular characteristics that require attention. The presence of resistance and virulence genes pose challenges for treatment selection and infection control measures.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Immunology
F. Morales-Leon, A. Opazo-Capurro, C. Caro, N. Lincopan, A. Cardenas-Arias, F. Esposito, V. Illesca, M. L. Rioseco, M. Dominguez-Yevenes, C. A. Lima, H. Bello-Toledo, Gerardo Gonzalez-Rocha
Summary: The convergence of virulence and multidrug-resistant phenotype is reported for the first time in Chile in Klebsiella pneumoniae complex isolates, indicating a clinical problem that requires special attention and continuous surveillance in South America. The detected hypervirulent isolates displayed resistance to broad-spectrum cephalosporins and carried multiple antibiotic-resistance genes, leading to a multidrug-resistant phenotype. In vivo virulence testing confirmed higher virulence of the isolates compared to the control strain.
Article
Biotechnology & Applied Microbiology
Kaylanne Montenegro, Claudia Flores, Ana Paula Alves Nascimento, Beatriz Oliveira Farias, Andressa S. Goncalves Brito, Mariana Magaldi, Aline Gimenez, Ivano de Filippis, Maysa Mandetta Clementino, Kayo Bianco, Enrico Saggioro, Paulo Barrocas
Summary: The occurrence and clonal relationships of carbapenem-resistant Klebsiella pneumoniae (CRKP) in hospital and municipal wastewater treatment plants (WWTPs) were investigated in this study. The results showed that among the 18 Kl. pneumoniae strains recovered, 39% were classified as multidrug-resistant (MDR), 61.1% were extensively drug-resistant (XDR), and 83.3% exhibited carbapenemase activity. Three carbapenemase-encoding genes (bla(KPC), bla(NDM), and bla(OXA-370)) were identified, and five sequence types (ST11, ST37, ST147, ST244, and ST281) were determined. It is important to monitor antimicrobial resistance in WWTPs effluents and use advanced treatment technologies to minimize the spread of bacterial load and antibiotic resistance genes in aquatic ecosystems.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Food Science & Technology
Fuat Aydin, Tuba Kayman, Secil Abay, Harun Hizlisoy, Izzet Burcin Saticioglu, Emre Karakaya, Orhan Sahin
Summary: This study investigated the genetic diversity, prevalence, and mechanisms of resistance to quinolone antibiotics in 178 Campylobacter jejuni isolates from humans, cattle, dogs, and chickens in Turkey. The results showed that there were 21 clonal complexes (CCs) and 78 different sequence types (STs) among the isolates, with CC21, CC353, CC206, and CC257 being the predominant clones. Quinolone resistance was detected in 41% of the isolates, and all resistant isolates had a specific mutation in the gyrA gene. The study provided important insights into the transmission pathways of C. jejuni in Turkey, suggesting that broiler meat and dogs may be the most important sources of human campylobacteriosis in the country.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Sabrina Nicolo, Giorgio Mattiuz, Alberto Antonelli, Fabio Arena, Vincenzo Di Pilato, Tommaso Giani, Ilaria Baccani, Ann Maria Clemente, Giuseppe Castronovo, Michele Tanturli, Federico Cozzolino, Gian Maria Rossolini, Maria Gabriella Torcia
Summary: The study suggests that hypervirulent Klebsiella pneumoniae (Hv-Kp) strains can interfere with the function of dendritic cells (DCs) and the differentiation of T cells, possibly evading immune response and contributing to pathogen dissemination in immunocompetent hosts.
Article
Biochemistry & Molecular Biology
Stefano Ravaioli, Davide Campoccia, Werner Ruppitsch, Franz Allerberger, Alessandro Poggi, Emanuele Chisari, Lucio Montanaro, Carla Renata Arciola
Summary: This study evaluated the usefulness of automated ribotyping as a screening tool for identifying S. aureus genetic lineages. Results showed that automated ribotyping can predict spa and MLST clonal complexes, but its high cost and lower discriminatory power could be obstacles for fine genotyping analysis. However, automated ribotyping has advantages such as automation, ease of execution, stability, typeability, and reproducibility, making it a reliable method to be used alongside gold standard methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Tamal Dey, Ardhendu Chakrabortty, Aastha Kapoor, Anuja Warrier, Vijaya Lakshmi Nag, Karthikeyan Sivashanmugam, Manoharan Shankar
Summary: Classical and hypervirulent strains of Klebsiella pneumoniae can cause different types of infections. The strain P34 isolated in India shows hypermucoviscosity but lacks known determinants of hypermucoviscosity, suggesting the presence of novel genetic factors.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Applied
Jinqiang Zhou, Bidong Wu, Miao Wang, Shujie Liu, Zhanxiong Xie, Chongwei An, Jingyu Wang
Summary: An effective method for preparing energetic microspheres by fluid-focusing microchannels is proposed, which combines the advantages of low reagent consumption, instrument miniaturization, accurate control, and efficient heat mass transfer. Compared with traditional water-suspension method, the microspheres prepared by the droplet microfluidic method show better morphology, particle size uniformity, and monodispersity. In addition, the fluidity and bulk density of the microspheres are significantly improved.
JOURNAL OF ENERGETIC MATERIALS
(2023)
Article
Chemistry, Applied
Wangjian Cheng, Kun Li, Lilong Gao, Chenhe Feng, Chongwei An, BaoYun Ye, Jingyu Wang
Summary: This study analyzed the distribution characteristics of the main explosive with different particle sizes and gradations using fractal theory and explored the effect of the gradation on the slurry. The results showed that particle size gradation can improve the fractal dimension of the explosive and reduce the thixotropic loop area of the slurry by increasing temperature and particle size distribution.
JOURNAL OF ENERGETIC MATERIALS
(2023)
Article
Agriculture, Dairy & Animal Science
Xian Liu, Peng Yang, Haoming Sun, Zijing Zhang, Cuicui Cai, Jiawei Xu, Xiaoting Ding, Xianwei Wang, Shijie Lyu, Zhiming Li, Zejun Xu, Qiaoting Shi, Eryao Wang, Chuzhao Lei, Hong Chen, Baorui Ru, Yongzhen Huang
Summary: This study found an association between copy number variation of the VAMP7 gene and growth traits in cattle. In Yunling cattle, the Duplication type of the gene was significantly related to height at the hip cross, with individuals having this type showing poorer growth and development. Individuals with other CNV types may be considered for breeding purposes.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Lixian Zou, Yuan Zheng, Jialing Chen, Yu Ding, Hui Liu, Yubao Liu, Jian Xu, Hairong Zheng, Xin Liu
Summary: Autocalibrated MB + CS-FPP improves the anatomical coverage of myocardial perfusion imaging while maintaining comparable image quality to conventional FPP. This is of great significance for the diagnosis of coronary artery disease.
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2023)
Article
Automation & Control Systems
Jianlin Wang, Xinquan Zhang
Summary: This article studies the problem of overcoming controller vulnerability in nonlinear continuous-time switched systems with saturating actuators. The sufficient condition for non-fragile stabilization of the system is derived using multiple Lyapunov functions. Then, a switching law and non-fragile state feedback controllers are designed to achieve asymptotic stabilization of the closed-loop system at the origin. Finally, the design issue of the controllers and switching law is transformed into a convex optimization problem with linear matrix inequalities (LMI) constraints, and a numerical example is provided to verify the effectiveness of the proposed method.
JOURNAL OF CONTROL AND DECISION
(2023)
Article
Clinical Neurology
Weilong Guo, Jin Liu, Bangshan Liu, Mi Wang, Qiangli Dong, Xiaowen Lu, Jinrong Sun, Liang Zhang, Hua Guo, Futao Zhao, Weihui Li, Zexuan Li, Mei Liao, Li Zhang, Yan Zhang, Yumeng Ju, Lingjiang Li
Summary: This study examines the impact of childhood maltreatment and major depressive disorder (MDD) on cognitive function in unmedicated patients. MDD significantly impairs cognitive function in all domains, while childhood maltreatment only affects memory. No interactive effect was found between MDD and childhood maltreatment.
EUROPEAN ARCHIVES OF PSYCHIATRY AND CLINICAL NEUROSCIENCE
(2023)
Article
Computer Science, Artificial Intelligence
Tingting Chai, Jiahui Li, Yanhong Wang, Guoying Sun, Changyong Guo, Zhaoxin Zhang
Summary: Finger-vein has been widely used in automatic personal recognition systems. This study investigates lightweight CNN models and the effect of image enhancement on finger-vein biometrics. Transfer learning plays an important role in extracting informative features. The experimental results show that the proposed lightweight model outperforms existing competition winners and image enhancement is not essential for CNN-based finger-vein biometrics.
NEURAL PROCESSING LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Jia-yun Ni, Xin Wang, Hong-yan Xie, Ning-hao Yang, Jing-yao Li, Xi-ang Sun, Heng-jiang Guo, Li Zhou, Wei Zhang, Jun Liu, Li-min Lu
Summary: Transforming growth factor-beta 1 (TGF-beta 1) plays a key role in promoting renal fibrosis during chronic kidney disease (CKD). Cellular senescence is associated with CKD progression, and accelerated tubular cell senescence contributes to renal fibrosis. The elevation of Usp11 under pathological condition is implicated in promoting renal fibrosis by deubiquitinating Tgfbr2, reducing its degradation, and facilitating downstream senescent signaling pathway activation.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Engineering, Environmental
Jiaqin He, Xunxun Li, Yaru Wang, Jun Xiao, Yunchong Liu, Hua Li, Najun Li, Qingfeng Xu, Jinghui He, Dongyun Chen, Jianmei Lu
Summary: A facile and eco-friendly strategy was developed to synthesize a series of Au-modified hollow Ni-Mn spinel nanospheres decorated on graphene aerogels (termed Auy-hNixMn3_x/GAz) composite catalysts for deep catalytic oxidation of Volatile organic compounds (VOCs). The introduction of hollow structure led to a larger specific surface area and more accessible active sites. The subsequent cooperation of Au further strengthened the low-temperature reducibility along with more active surface oxygen species.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Cardiac & Cardiovascular Systems
Jing Li, Juan Zhang, Yu Shi, Horst Sievert, Cynthia C. Taub, Stefan Bertog, Shengjun Ta, Lei Changhui, Ethan Senser, Jing Wang, Rui Hu, Junzhe Huang, Fangqi Ruan, Yupeng Han, Xiaojuan Li, Bo Wang, Xueli Zhao, Jiao Liu, David H. Hsi, Liwen Liu
Summary: PIMSRA is an effective treatment option for hypertrophic obstructive cardiomyopathy (HOCM), leading to improved exercise capacity, reduced left ventricular outflow tract gradients, and increased myocardial contractile function.
Article
Chemistry, Organic
Lixia Ding, Zhifei Zhu, Xinyue Zhou, Gongming Zhu, Jian-Hua Wang, Shu-Tong Zhu, Bing Hu, Xiao-Na Wang, Junbiao Chang
Summary: We present two efficient intermolecular self-cycloadditions of N-aryl-substituted ynamides via metal-free approaches, resulting in the synthesis of valuable aminocyclobutenes and 4-aminoquinoline derivatives with good yields and wide substrate scope. Our modified method allowed for the effective preparation of terminal N-aryl ynamides, which then underwent thermally driven and solvent-free [2 + 2] cycloaddition to form functionalized aminocyclobutenes, or TMSOTf/TfOH-catalyzed [4 + 2] cycloaddition to produce 4-aminoquinoline derivatives. Furthermore, evaluation of their in vitro activity revealed that some of the cycloaddition products and their derivatives exhibited highly potent activities against SARS-CoV-2, DENV-2, and tumor cell lines.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Computer Science, Artificial Intelligence
Guangming Wang, Xinrui Wu, Shuyang Jiang, Zhe Liu, Hesheng Wang
Summary: This paper proposes an efficient 3D point cloud learning architecture called EfficientLO-Net for LiDAR odometry. It introduces the projection-aware representation of the 3D point cloud to achieve efficiency. The Pyramid, Warping, and Cost volume (PWC) structure is built to estimate and refine the pose. The architecture incorporates a projection-aware attentive cost volume, a trainable embedding mask, and a trainable pose warp-refinement module to improve the pose estimation and filter outlier points.
IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE
(2023)
Article
Chemistry, Physical
Shuaishuai Shang, Shuhao An, He Li, Jianping Li, Shenping Zhang, Changjun Peng, Honglai Liu, Jun Hu
Summary: We developed a sulfonate-based covalent organic framework (sCOF) for the fast and sensitive detection of tetracycline hydrochloride (TCH). By incorporating disulfonic acid into COF, we achieved self-exfoliation of the framework and obtained fluorescent covalent organic nanosheets (f-sCON). These f-sCON nanosheets showed excellent sensitivity towards TCH residuals and could be used to create a naked-eye fluorescent device. Furthermore, by combining sulfonate-COF adsorbent and f-sCON-gel in a mixed column, we successfully achieved simultaneous TCH removal and detection.
JOURNAL OF MATERIALS CHEMISTRY A
(2023)
Article
Hematology
Lili Song, Peifeng Li, Huiying Sun, Lixia Ding, Jing Wang, Benshang Li, Bin-Bing S. Zhou, Haizhong Feng, Yanxin Li
Summary: This study found that mutations in phosphoribosyl pyrophosphate synthetase 2 (PRPS2) drive relapse of childhood acute lymphoblastic leukemia (ALL) by influencing the stability of PRPS1/2 hexamers. Mutations in PRPS2 decrease the effectiveness of thiopurine therapy in relapsed childhood ALL. This discovery provides important clues for finding biomarkers for relapsed ALL.
Article
Chemistry, Physical
Yanlin Feng, Jianlin Wang, Xin Ning, Aiyun Li, Qing You, Wanzhen Su, Deping Wang, Jianyun Shi, Lan Zhou, Fangfang Cao, Xiaoyuan Chen, Jimin Cao
Summary: We developed a stable pyroelectric BaTiO3@Au core-shell nanostructure that can selectively block energy generation of tumor cells, providing a promising therapeutic approach for triple-negative breast cancer.