期刊
JOURNAL OF SEPARATION SCIENCE
卷 43, 期 22, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202000514
关键词
leucopenia; pharmacokinetics; phenolic acid; Sanguisorba officinalisL; ultra high performance liquid chromatography
资金
- ScientificResearch Project of National Natural Science Foundation of China [81573551, 81973552]
A selective, accurate, and efficient liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of 13 phenolic acids. Additionally, for more comprehensively determining the chemical constituents inSanguisorba officinalisL. extract, a previously developed method was employed for the simultaneous determination of six triterpenes. Thus, two methods were used to ensure the comprehensiveness and reliability of this study. Based on these methods, the pharmacokinetic profiles of the 13 phenolic acids and 6 triterpenes in normal and leukopenia rats after oral administration ofS. officinalisL. extract were compared for the first time in the present study. Quantitative detection of the 13 phenolic acids and 6 triterpenes was performed using the multiple reaction monitoring mode with the electrospray ion source in negative and positive electrospray ionization, respectively. Chromatographic separation was performed on an Agilent Eclipse Plus C18 RRHD column (50 x 2.1 mm, 1.8 mu m) using gradient elution with a mobile phase composed of methanol-0.1% aqueous formic acid. The pharmacokinetic results demonstrated that the pharmacokinetic characteristics of the 19 analytes in leukopenia rats differed significantly from those determined in normal rats, which could provide a helpful reference for the clinical application ofS. officinalisL. in the prevention and treatment of leucopenia.
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