Article
Plant Sciences
Jingxiong Zhang, Yuxing Xu, Jing Xie, Huifu Zhuang, Hui Liu, Guojing Shen, Jianqiang Wu
Summary: Dodder is a parasitic plant that can transfer nitrogen systemic signals between hosts with different nitrogen levels, regulating transcriptome and methylome changes in the recipient hosts. This study also found that under nitrogen stress conditions, dodder facilitates bilateral nitrogen systemic signaling between different hosts, impacting inter-plant mobile mRNAs.
Article
Plant Sciences
Qing Zhang, Aiwen Deng, Min Xiang, Qiuyan Lan, Xiaokun Li, Shuai Yuan, Xin Gou, Shuang Hao, Juan Du, Chaowen Xiao
Summary: Pectin, cellulose, and hemicellulose are important components of the primary cell wall in eudicots and play a vital role in plant development. This study found that PGX2 is involved in regulating root hair development, and its activation tagging affects cell wall remodeling, auxin signaling, and actin microfilament orientation.
FRONTIERS IN PLANT SCIENCE
(2022)
Editorial Material
Biochemistry & Molecular Biology
Deirdre H. Mclachlan
Summary: Long-distance wound signalling in plants involves systemic propagation of calcium waves, and a new study proposes a mechanism whereby pressure changes trigger this response.
Article
Biochemistry & Molecular Biology
Ranju Chutia, Sarah Scharfenberg, Steffen Neumann, Steffen Abel, Joerg Ziegler
Summary: The research reveals the important roles of iron and phosphate in plant growth and metabolic regulation, showing their interdependency and interaction which affect plant growth and development under different nutrient conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Chang-Yi Chiu, Hui-Fang Lung, Wen-Chun Chou, Li-Yen Lin, Hong-Xuan Chow, Yu-Hao Kuo, Pei-Shan Chien, Tzyy-Jen Chiou, Tzu-Yin Liu
Summary: Autophagy in plants is regulated by different signaling cascades in response to environmental changes. Fine-tuning of its activity is critical for cellular homeostasis. This study observed that Pi deficiency moderately upregulates most ATG genes and increases autophagic structures in the root of Arabidopsis, but does not significantly alter autophagic flux in the whole roots. At the organismal level, mutants of ATG exhibited decreased shoot Pi concentrations and smaller meristem sizes under Pi sufficiency.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Biology
Chao Ma, Qiuju Chen, Shiping Wang, Amnon Lers
Summary: The microRNA miR827 and the transcription regulator GPL alpha are functionally involved in both senescence and phosphate homeostasis, with miR827 playing a role in regulating the expression of GPL alpha. The expression of GPL alpha is induced by phosphate deficiency and overexpression of GPL alpha leads to reduced expression of phosphate transporter genes. The close interactions between senescence and phosphate homeostasis are further emphasized by the functional involvement of miR827 and GPL alpha in both processes.
Article
Multidisciplinary Sciences
Devesh Shukla, Prabodh Kumar Trivedi, Shivendra Sahi
Summary: Comprehensive understanding of plant root system architecture (RSA) development is crucial for enhancing nutrient use efficiency and increasing crop cultivar tolerance to environmental challenges. This study presents an experimental protocol for establishing a hydroponic system, promoting plantlet growth, spreading and imaging the RSA. The method utilizes a magenta box-based hydroponic system supported by polypropylene mesh and polycarbonate wedges. The approach was initially designed for assessing the RSA of Arabidopsis, but can be easily adapted for studying other plants such as Medicago sativa (Alfalfa). The measurement of RSA traits is achieved through capturing high-resolution images of the carefully spread root systems using freely available ImageJ software.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Plant Sciences
Samriti Mankotia, Dhriti Singh, Kumari Monika, Muskan Kalra, Himani Meena, Varsha Meena, Ram Kishor Yadav, Ajay Kumar Pandey, Santosh B. Satbhai
Summary: Iron is essential for plants and animals, and its limitation significantly affects crop yield and human nutrition. Plants have adapted strategies to regulate iron uptake and homeostasis, as well as modify root system architecture to survive under low iron conditions. Understanding the molecular mechanism of root growth responses has implications for plant breeding.
Article
Plant Sciences
Amber Mertens, Nele Horemans, Eline Saenen, Robin Nauts, Ann Cuypers
Summary: This study found that calcium influences uranium uptake and toxicity in the terrestrial plant Arabidopsis thaliana, affecting plant growth and oxidative stress. Uranium, in turn, influences calcium uptake and distribution. The expression of calcium transporters is also influenced by uranium and calcium channel blockers.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Hui Song, Qingliu Geng, Xi Wu, Min Hu, Min Ye, Xin Yu, Yifan Chen, Jiena Xu, Li Jiang, Shuqing Cao
Summary: This study identified MYC1 as a negative regulator of Fe-deficiency stress in Arabidopsis thaliana. MYC1 inhibits the formation of FIT and bHLH38/39 heterodimers, suppressing the expression of Fe acquisition genes FRO2 and IRT1. These findings provide potential targets for developing crop varieties resistant to Fe-deficiency stress.
Article
Multidisciplinary Sciences
Melissa Bredow, Kyle W. Bender, Alexandra Johnson Dingee, Danalyn R. Holmes, Alysha Thomson, Danielle Ciren, Cailun A. S. Tanney, Katherine E. Dunning, Marco Trujillo, Steven C. Huber, Jacqueline Monaghan
Summary: Phosphorylation of Ser318 is crucial for the activation of CPK28 in immune homeostasis and stem elongation, providing protection against immune response initiation in the absence of infection. In contrast, Ser318 phosphorylation is not necessary for stem elongation, indicating pathway-specific requirements for phosphorylation-based Ca2+-sensitivity priming. This research also suggests a potential biotechnological application in enhancing resistance to microbial pathogens without affecting yield through conserved Ser318 phosphorylation in related group IV CDPKs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Yi Ma, Keila Garrido, Rashid Ali, Gerald A. Berkowitz
Summary: Plant CNGCs play a crucial role in cytosolic Ca2+ influx for immune defense and thermosensing. Physically interacting CNGCs (CNGC2, CNGC4, and CNGC6) may form a multimeric channel complex involved in immune signaling, while CNGC12 does not interact with other CNGCs. CNGC2, CNGC4, and CNGC6 mutants showed impaired response to the elicitor peptide Pep3, whereas all four CNGC mutants exhibited thermotolerance.
Article
Biochemistry & Molecular Biology
Naga Jyothi Pullagurla, Supritam Shome, Ranjana Yadav, Debabrata Laha
Summary: Jasmonic acid (JA) is a plant hormone that regulates various physiological processes. Inositol pyrophosphates (PP-InsPs) play a role in JA perception. The ITPK1 kinase is involved in JA-dependent root architecture development by controlling the expression of different JAZ repressor proteins.
Article
Biotechnology & Applied Microbiology
Yusuke Shikanai, Mayu Asada, Takafumi Sato, Yusuke Enomoto, Mutsumi Yamagami, Katsushi Yamaguchi, Shuji Shigenobu, Takehiro Kamiya, Toru Fujiwara
Summary: In this study, a novel gene GSL8 was identified to be required for low Ca tolerance in Arabidopsis thaliana. The GSL genes were found to play important roles in low Ca tolerance and GSL8 and GSL10 exhibited additive effects in this process.
PLANT BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Estefania Mancini, Antoni Garcia-Molina
Summary: This study investigated the effect of alternative splicing (AS) on the transcriptome of rosette leaves under single and simultaneous copper (Cu) and iron (Fe) deficiency. It was found that differentially spliced genes (DSGs) and differentially expressed genes (DEGs) showed minimal overlap in number, indicating distinct responses to the double deficiency. Furthermore, it was observed that AS might act as a regulatory mechanism to modify the stability and/or functionality of enzymes involved in amino acid production during the combinatorial response to simultaneous Cu and Fe deficiency.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Hang Su, Tian Wang, Chuanfeng Ju, Jinping Deng, Tianqi Zhang, Mengjiao Li, Hui Tian, Cun Wang
Summary: This study demonstrates the important role of ABA signaling components, particularly SnRK2 proteins, in root foraging and nitrate uptake in Arabidopsis thaliana under nitrogen deficiency. SnRK2 proteins interact with and phosphorylate the nitrate transceptor NRT1.1, leading to decreased nitrate uptake and impaired root growth. The identification of a previously unknown functional site on NRT1.1 provides new insights into how plants regulate growth under nitrogen deficiency via ABA signaling.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhenqian Zhang, Dali Fu, Zhihui Sun, Chuanfeng Ju, Cuicui Miao, Zhangqing Wang, Dixiang Xie, Liang Ma, Zhizhong Gong, Cun Wang
Summary: The study identified a tonoplast-associated calcium signaling cascade that regulates manganese homeostasis in plants, linking manganese toxicity, calcium signaling, and manganese transporters. The calcium-dependent protein kinases CPK4/5/6/11 were found to interact with the Mn and Fe transporter MTP8, with phosphorylation at the Ser31 and Ser32 residues crucial for MTP8 function. These findings provide new insights into manganese homeostasis mechanisms and potential targets for engineering heavy metal toxicity-tolerant plants.
Article
Biochemistry & Molecular Biology
Chuanfeng Ju, Zhenqian Zhang, Jinping Deng, Cuicui Miao, Zhangqing Wang, Lukas Wallrad, Laiba Javed, Dali Fu, Ting Zhang, Jorg Kudla, Zhizhong Gong, Cun Wang
Summary: Manganese is an essential micronutrient for plants, but excess supply can be toxic. This study reveals a two-tiered calcium-controlled mechanism that regulates plant manganese homeostasis.
Article
Multidisciplinary Sciences
Dali Fu, Zhenqian Zhang, Lukas Wallrad, Zhangqing Wang, Stefanie Hoeller, ChuanFeng Ju, Ina Schmitz-Thom, Panpan Huang, Lei Wang, Edgar Peiter, Joerg Kudla, Cun Wang
Summary: The homeostasis of essential micronutrient manganese (Mn) in all organisms is determined by its availability and uptake efficiency. This study reveals that manganese depletion induces long-lasting Ca2+ oscillations in the roots of Arabidopsis, which are regulated by the Ca2+-dependent protein kinases CPK21 and CPK23. These oscillations control the uptake activity of the high-affinity Mn transporter NRAMP1 through translation of the Ca2+ signals. Thr498 phosphorylation within NRAMP1 is identified as a key regulatory mechanism for its activity. These findings highlight the importance of the Ca2+-CPK21/23-NRAMP1 axis in plant Mn homeostasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Yanting Zhang, Zhangqing Wang, Yisong Liu, Tianqi Zhang, Jiaming Liu, Zhang You, Panpan Huang, Zhenqian Zhang, Cun Wang
Summary: Cadmium (Cd) is a toxic heavy element that hampers plant growth. In this study, we investigated the mechanisms by which plants sense and cope with Cd stress. We discovered that Cd stress induces calcium signals in the roots of Arabidopsis plants. Through protein interaction techniques, we identified CPK21 and CPK23 as calcium-dependent protein kinases that interact with the Cd transporter NRAMP6. Further experiments showed that the phosphorylation of NRAMP6 by CPK21/23 inhibits its Cd transport activity, thereby improving plant tolerance to Cd stress.
Article
Plant Sciences
Zhenqian Zhang, Dali Fu, Dixiang Xie, Zhangqing Wang, Yuqing Zhao, Xuening Ma, Panpan Huang, Chuanfeng Ju, Cun Wang
Summary: This study identified the CBL1/9-CIPK23-NRAMP1 module as a regulator of tolerance to high-Mn toxicity in plants. It also provided insights into the mechanism of plant tolerance to Mn toxicity.
Article
Plant Sciences
Tian Wang, Mengjiao Li, Jize Yang, Min Li, Zhenqian Zhang, Huiling Gao, Cun Wang, Hui Tian
Summary: BRs are involved in plant response to nitrate deficiency and regulate the expression of high affinity nitrate transporters, where the transcription factor BES1 directly binds to the promoters of NRT2.1 and NRT2.2 to promote their expression under nitrate deficiency.
Editorial Material
Plant Sciences
Peng Wang, Dali Fu
Article
Plant Sciences
Yisong Liu, Yanting Zhang, Zhangqing Wang, Shiyuan Guo, Yanjun Fang, Zhenqian Zhang, Huiling Gao, Huimin Ren, Cun Wang
Summary: Arsenate (As[V]) is a widely distributed metalloid in various environments with heavy metal properties. It was found that As(V) stress induces a significant calcium signal in Arabidopsis roots, and a calcium-dependent protein kinase (CPK23) was identified to interact with the plasma membrane As(V)/Pi transporter PHT1;1. Mutants lacking CPK23 exhibited a sensitive phenotype under As(V) stress, while plants with constitutively active CPK23 showed tolerance. CPK23 phosphorylated the C-terminal domain of PHT1;1 at Ser514 and Ser520, and the phosphorylation at Ser514 was found to be essential for PHT1;1 function and localization under As(V) stress. The study reveals the role of plasma-membrane-associated calcium signaling in regulating As(V) tolerance and provides insights for crop bioengineering to address arsenate pollution.
Article
Biology
Zhangqing Wang, Yanting Zhang, Yisong Liu, Dali Fu, Zhang You, Panpan Huang, Huiling Gao, Zhenqian Zhang, Cun Wang
Summary: Iron (Fe) is essential for all organisms, but its availability in the soil is often insufficient for plant growth, leading to iron deficiencies that affect crop yield. This study investigates the role of calcium (Ca2+) in regulating iron deficiency and identifies two calcium-dependent protein kinases (CPK21 and CPK23) that play a critical role in plant tolerance to iron-deficient conditions. The study also reveals the interaction and phosphorylation of the iron transporter IRT1 by CPK21 and CPK23, and identifies a critical residue (Ser149) for IRT1 transport activity.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Zhang You, Shiyuan Guo, Qiao Li, Yanjun Fang, Panpan Huang, Chuanfeng Ju, Cun Wang
Summary: This study reveals a functional module of the CBL1/9-CIPK1-PYLs regulatory network that plays a negative role in ABA signaling and its response to drought stress.
NATURE COMMUNICATIONS
(2023)