Article
Physics, Applied
Yuting Gao, Zhijie Liu, Sitao Wang, Bolun Pang, Dehui Xu, Dingxin Liu, Michael G. Kong
Summary: The study found that the oil layer significantly impedes the permeation of RONS, leading to a reduction in the formation of aqueous RONS in the PAOW system. This mechanism may be due to the reaction of RONS with unsaturated bonds in the oil or adsorption by the high viscosity of the oil.
PLASMA PROCESSES AND POLYMERS
(2021)
Article
Physics, Applied
Zhijie Liu, Xiamin Tantai, Sitao Wang, Bolun Pang, Yuting Gao, Feng Zhang, Xinyi Zhao, Dehui Xu, Aswathi Soni, Anne Mai-Prochnow, Patrick Cullen, Renwu Zhou
Summary: This study investigated the physicochemical properties and anticancer effects of plasma-activated solutions (PAS), showing that different solutions significantly affected the gas-liquid interaction reactions and the color penetration distribution of H2O2 and NO2-. The anticancer effects of PAS were found to be in the order of DI > PB approximate to saline > PBS > RPMI 1640, attributed to the important role of H2O2 and NO2- in inactivating cancer cells. This research provides insights for designing and applying PAS in practical anticancer therapy.
PLASMA PROCESSES AND POLYMERS
(2023)
Article
Food Science & Technology
Koentadi Hadinoto, Javiera Barrales Astorga, Hassan Masood, Renwu Zhou, David Alam, Patrick J. Cullen, Stuart Prescott, Francisco J. Trujillo
Summary: By optimizing the chemistry, antimicrobial efficacy and energy consumption of plasma-activated water (PAW), high inactivation rates of more than 5-log(10) reductions were achieved against Escherichia coli and Salmonella Typhimurium. This technology shows promising potential as an eco-friendly alternative to chemical disinfection, with excellent energy efficiency in generating reactive oxygen and nitrogen species (RONS). Its industrial application and scale-up can be supported through further research on PAW reactor design and operation.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2021)
Article
Engineering, Environmental
Koentadi Hadinoto, N. R. H. Rao, Javiera Barrales Astorga, Renwu Zhou, Joanna Biazik, Tianqi Zhang, Hassan Masood, Patrick J. Cullen, Stuart Prescott, Rita K. Henderson, Francisco J. Trujillo
Summary: This article introduces a newly designed hybrid plasma discharge reactor that produces plasma-activated water (PAW), an effective and energy-efficient disinfectant. By adjusting parameters such as the number and size of orifices in the bubble column, liquid volume, and salinity, the inactivation of Escherichia coli can be further improved.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
N. R. H. Rao, X. Chu, K. Hadinoto, Angelina Angelina, R. Zhou, T. Zhang, B. Soltani, C. G. Bailey, F. J. Trujillo, G. L. Leslie, S. W. Prescott, P. J. Cullen, R. K. Henderson
Summary: This study evaluated the efficacy of cold plasma-activated bubbles in treating algal-impacted waters. The results showed that both air and oxygen-activated bubbles were effective in reducing cell numbers and increasing cell damage and inactivation. However, oxygen-activated bubbles were preferred due to lower nitrite and nitrate concentrations in the treated water. The study also found that different reactive species had varying impacts on algal cell removal and damage.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Se Hoon Ki, Kai Masur, Ku Youn Baik, Eun Ha Choi
Summary: This study analyzed the penetration of active species from NTAPP through a skin model using UV absorption spectroscopy and quantified the diffusion of hydrogen peroxide, nitrite, and nitrate through curve fitting. The research utilized an agarose gel to mimic epidermis and dermis layers, along with lipid film or pig skin sample to mimic the stratum corneum. The diffusion characteristics of reactive species through this skin model and the limitations of the method were discussed.
APPLIED SCIENCES-BASEL
(2021)
Review
Biochemistry & Molecular Biology
Yuhan Yang, Yuan Wang, Shang Wei, Xianzhong Wang, Jiaojiao Zhang
Summary: Non-thermal plasma (NTP) is an ionized gas composed of reactive species, electric fields, and ultraviolet radiation. It can affect animal health by mediating reactive oxygen species (ROS) levels and has a wide range of potential applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physics, Applied
Bolun Pang, Zhijie Liu, Huaiyan Zhang, Sitao Wang, Yuting Gao, Dehui Xu, Dingxin Liu, Michael G. Kong
Summary: The study found that PAW has a higher inactivation efficiency at 25 degrees Celsius, while the physicochemical properties and the concentration of reactive oxygen and nitrogen species of PAW significantly decrease when the temperature exceeds 25 degrees Celsius. Research on the temperature effect during PAW production is beneficial for optimizing production efficiency.
PLASMA PROCESSES AND POLYMERS
(2022)
Article
Electrochemistry
Alexander G. Volkov, Asya Bookal, Jewel S. Hairston, Jade Roberts, Gamaliel Taengwa, Darayas Patel
Summary: This study found that a cold atmospheric pressure He-plasma jet can oxidize N-2 to HNO3 and HNO2, inducing redox reactions at the plasma-air/water interface and in the volume of the aqueous phase. The plasma activated water can be utilized in various fields such as medicine, food industry, and agriculture for disinfection, sterilization, and production of nitrogen compounds, offering a potential alternative to expensive traditional processes.
ELECTROCHIMICA ACTA
(2021)
Article
Food Science & Technology
Siyao Ju, Bing Li, Xudong Yang, Jingjing Wu, Xuekai Pei, Jie Zhuang, Ruonan Ma, Zhen Jiao, Rusen Zhou, Patrick J. Cullen
Summary: In this study, a novel green fungicide named plasma-activated water (PAW) was prepared by using air, water, and electricity via cold plasma activation. The antifungal effects of PAW against Fusarium graminearum spores were investigated, and it was found that PAW achieved a significant reduction in spore quantity and inhibited mycelial growth, spore germination, and fungal pathogenicity. Hydroxyl radicals and hydrogen peroxide were identified as the major antifungal agents in PAW, which caused cell necrosis. Field-plot experiments demonstrated that PAW effectively controlled Fusarium head blight in wheat. The results indicate that PAW has great potential as an efficient and eco-friendly fungicide for FHB control in wheat.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Plant Sciences
Enrico Cortese, Alessio G. Settimi, Silvia Pettenuzzo, Luca Cappellin, Alessandro Galenda, Alessia Famengo, Manuele Dabala, Vanni Antoni, Lorella Navazio
Summary: This study analyzed the signaling mechanisms underlying plant response to plasma-activated water (PAW), revealing that PAW can induce rapid and sustained elevations of cytosolic Ca2+ in Arabidopsis seedlings, with the dynamics of the changes depending on various parameters. The unique mixture of reactive chemical species in PAW was found to be responsible for the specific Ca2+ signatures, as separate administration of nitrate, nitrite, and hydrogen peroxide did not trigger detectable Ca2+ changes. Understanding the plant perception of PAW may enable fine-tuning for agricultural applications, potentially leading to more sustainable agriculture.
Article
Biochemistry & Molecular Biology
Rafael Silva-Teixeira, Mafalda Laranjo, Beatriz Lopes, Catarina Almeida-Ferreira, Ana Cristina Goncalves, Tiago Rodrigues, Paulo Matafome, Ana Bela Sarmento-Ribeiro, Francisco Caramelo, Maria Filomena Botelho
Summary: The study evaluated the therapeutic effect and mechanisms of cold atmospheric plasma (CAP) on human retinoblastoma cells, showing that both direct and indirect treatment decreased cell survival and affected cell cycle without causing DNA damage. Reactive species (RS) increased in two waves, with additional carboplatin enhancing cytotoxicity and achieving maximal antiangiogenic effects with 60 seconds of plasma exposure.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Lea Miebach, Eric Freund, Ramona Clemen, Klaus-Dieter Weltmann, Hans-Robert Metelmann, Thomas von Woedtke, Torsten Gerling, Kristian Wende, Sander Bekeschus
Summary: Gas plasma jet technology has been found to have potential in palliative treatment for cancer patients. Direct contact with tumor tissue showed higher therapeutic efficacy compared to remote treatment with reactive oxygen and nitrogen species. The study emphasizes the importance of standardized treatment distances in gas plasma exposure to cancer therapy.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Nanoscience & Nanotechnology
Nishtha Gaur, Bethany L. Patenall, Bhagirath Ghimire, Naing T. Thet, Jordan E. Gardiner, Krystal E. Le Doare, Gordon Ramage, Bryn Short, Rachel A. Heylen, Craig Williams, Robert D. Short, Toby A. Jenkins
Summary: We introduce a versatile drug-loaded composite hydrogel that can simultaneously deliver a drug and CAP-generated molecules in a tissue target when activated by an argon-based cold atmospheric plasma jet. We demonstrated this concept by encapsulating gentamicin in sodium polyacrylate particles dispersed within a poly(vinyl alcohol) hydrogel matrix. The resulting gentamicin-PAA-PVA composite hydrogel can release gentamicin and effectively eradicate bacteria, including those in planktonic state and biofilm, when activated by CAP. The concept of the composite hydrogel can also be applied to other antimicrobial agents like cetrimide and silver, and activated by any dielectric barrier discharge CAP device.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Environmental
Hangbo Xu, Chao Liu, Qing Huang
Summary: In this study, the authors investigated the inactivation effect of cold atmospheric plasma (CAP) combined with plasma-activated water (PAW) treatment on two types of fungi. The results showed that the combination of short-term CAP treatment and long-term PAW treatment significantly reduced the fungi compared to the single CAP approach. Comparative analysis revealed that different reactive oxygen and nitrogen species played a role in the inactivation of fungi during the combination treatment. The synergistic effect was attributed to the increasing intracellular reactive species-induced damage to the fungal membrane and mitochondria. This study provides new insights into the optimal application of low-temperature plasma technology in fungal disinfection.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Chemical
Miao Tian, Dehui Xu, Bing Li, Shuai Wang, Miao Qi, Hao Zhang, Zhijie Liu, Dingxin Liu, Hailan Chen, Michael G. Kong
Summary: Cold atmospheric plasma (CAP) is a promising anti-cancer treatment modality, but the mechanism of its selective cytotoxicity requires further investigation. Metabolite analysis revealed that A375 melanoma cells are more sensitive to CAP treatment, with changes in purine metabolism and Pantothenate and CoA biosynthesis potentially playing a role.
PLASMA CHEMISTRY AND PLASMA PROCESSING
(2021)
Article
Physics, Applied
Jishen Zhang, Bing Li, Shengduo Xu, Dingxin Liu, Hao Zhang, Dehui Xu, Li Guo, Michael G. Kong
Summary: The study found that cold plasma jet exhibited selectivity when combined with specific anticancer drugs, leading to varying anticancer effects in different combinations. Plasma jet combined with paclitaxel showed the strongest anticancer effect, followed by adriamycin, cisplatin, and methotrexate.
PLASMA PROCESSES AND POLYMERS
(2021)
Article
Oncology
Hao Zhang, Jishen Zhang, Bo Guo, Hailan Chen, Dehui Xu, Michael G. Kong
Summary: Bladder cancer is the most common urinary system carcinoma, with muscle-invasive bladder cancer (MIBC) being a fatal clinical problem for most patients. Plasma-activated saline (PAS) shows potential as a therapeutic approach for MIBC, inducing cancer cells' apoptosis by activating the ROS-mediated Fas/CD95 pathway.
Article
Engineering, Chemical
Hao Zhang, Jishen Zhang, Shengduo Xu, Yifan Liu, Bowen Sun, Zifeng Wang, Dehui Xu, Li Guo, Dingxin Liu, Michael G. Kong
Summary: CAP and PEF, two emerging anticancer technologies, have different mechanisms in inducing apoptosis in cancer cells. PEF mainly targets cells in the S and G(2)/M phases and shows effects within the first four hours of treatment, while the He + O-2 plasma jet induces apoptosis by increasing cellular oxidative stress and maintains effects for at least 24 hours after irradiation.
PLASMA CHEMISTRY AND PLASMA PROCESSING
(2021)
Article
Physics, Applied
Hao Zhang, Shengduo Xu, Jishen Zhang, Bing Li, Dingxin Liu, Li Guo, Zhijie Liu, Dehui Xu
Summary: The study demonstrates that the synergistic anticancer effect of plasma jet and doxorubicin depends on their combination method. Continuous doxorubicin treatment followed by plasma jet treatment shows significantly better results than simultaneous treatment, suggesting the potential for creating transient therapeutic windows for continuous chemotherapy. These findings will help optimize therapeutic approaches involving a combination of anticancer drugs and plasma for future clinical use.
PLASMA PROCESSES AND POLYMERS
(2021)
Article
Nanoscience & Nanotechnology
Yifan Liu, Dingxin Liu, Jishen Zhang, Bowen Sun, Santu Luo, Hao Zhang, Li Guo, Mingzhe Rong, Michael G. Kong
Summary: The study of plasma-liquid interaction is crucial, and the results from three fluid models show that the distribution of reactive species is largely unaffected by interfacial boundary conditions in both gas and liquid phases. However, reactive species with different Henry's law constants exhibit significant differences in interfacial gas layers. Only reactive species with low Henry's law constants are affected by uncertainty in the models.
Article
Physics, Applied
Han Xu, Shaoshuai Guo, Hao Zhang, Kai Xie
Summary: In this study, the impact of an external axial magnetic field on helium plasma jet and plasma-activated water was investigated. It was found that the external magnetic field significantly enhances the brightness and length of the ionization wave of the plasma jet, while also increasing the concentration of reactive oxygen species in water. Additionally, the vitality of melanoma cells decreases under the influence of the magnetic field. The findings suggest that the external magnetic field could be beneficial in improving the effectiveness of plasma jet applications.
APPLIED PHYSICS LETTERS
(2021)
Article
Engineering, Biomedical
Hao Zhang, Shengduo Xu, Jishen Zhang, Zifeng Wang, Dingxin Liu, Li Guo, Cheng Cheng, Yilong Cheng, Dehui Xu, Michael G. Kong, Mingzhe Rong, Paul K. Chu
Summary: The plasma-activated biogel is capable of eliminating residual tumor tissues and preventing in situ recurrence after removal surgery with no evident systemic toxicity. It possesses excellent storage capability, allows for slow release of plasma-generated reactive oxygen species (ROS), and exhibits good ROS-mediated anticancer effects in vitro.
Article
Physics, Applied
Hao Zhang, Jishen Zhang, Shengduo Xu, Dingxin Liu, Li Guo, Hua Li
Summary: This study proposed a 7 μm micro-plasma jet device and evaluated its anticancer effects on three-dimensional bladder micro-tumor spheroids (T24 cell line) constructed in vitro. The experimental results showed that the device can effectively inactivate micro-tumors with diameters ranging from 50 to 300 μm, providing a new approach for precise micro-tumor therapy in clinical medicine.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Physics, Applied
Hao Zhang, Min Chen, Lingling Huang, Li Guo, Shengduo Xu, Jishen Zhang, Wang Xi, Zifeng Wang, Dingxin Liu, Michael G. Kong, Mingzhe Rong
Summary: The study demonstrates that plasma-treated air can effectively inhibit the infectivity of COVID-19 pseudovirus, whether attached to plastic or copper. Furthermore, in a cold-chain environment, plasma-generated reactive species are more efficient in deactivating wet pseudovirus compared to ozone.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Physics, Applied
Hao Zhang, Jishen Zhang, Shengduo Xu, Zifeng Wang, Dehui Xu, Li Guo, Dingxin Liu, Michael G. Kong, Mingzhe Rong
Summary: The combination of plasma-treated medium and hyperthermia shows better antitumor effects on bladder tumors, promoting the penetration of reactive oxygen species into cancer cells. This novel strategy may serve as a valuable therapeutic option for bladder cancer.
PLASMA PROCESSES AND POLYMERS
(2021)
Article
Physics, Applied
Dingxin Liu, Zifeng Wang, Zeyu Chen, Dehui Xu, Min Chen, Jinkun Chen, Mengying Zhu, Hao Zhang, Xiaohua Wang, Michael G. Kong, Mingzhe Rong
Summary: Cold atmospheric plasma and electrochemical treatment were compared for their anticancer effects, with plasma producing more reactive species leading to stronger inactivation of cancer cells. This suggests that electrochemistry does not dominate the plasma inactivation process.
PLASMA PROCESSES AND POLYMERS
(2021)
Article
Physics, Multidisciplinary
Yujing Xu, Sansan Peng, Bing Li, Shuai Wang, Hao Zhang, Qiaosong Li, Zhijie Liu, Bo Guo, Dingxin Liu, Dehui Xu
Summary: The study demonstrates that PAL can be safely injected into the bone marrow of New Zealand rabbits without causing significant toxicity, as evidenced by various tests including acute toxicity, organ examination, and blood analysis.
FRONTIERS IN PHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Miao Qi, Dehui Xu, Shuai Wang, Bing Li, Sansan Peng, Qiaosong Li, Hao Zhang, Runze Fan, Hailan Chen, Michael G. Kong
Summary: In recent years, cold atmospheric pressure plasma (CAP) technology has rapidly developed, along with the medical applications of cold plasma in cancer, skin disease, and tissue repair. Plasma-activated liquids are being studied as a potential alternative to CAP in cancer treatment. This study showed that plasma-activated saline (PAS) treatment effectively inhibited tumor growth in melanoma, with little effect of a low-pH environment. Glycerophospholipid metabolic pathway was found to be the most susceptible to PAS treatment in melanoma. PAS also demonstrated inhibition of cell proliferation in oral tongue squamous-cell cancer and non-small-cell lung cancer. The treatment was considered safe with minimal toxic side effects.