Review
Pharmacology & Pharmacy
Senwang Zheng, Songtao Liu, Ajiao Hou, Song Wang, Yexin Na, Jianhua Hu, Hai Jiang, Liu Yang
Summary: Lonicerae Japonicae Flos has been used for over 1,500 years as a tea and medicine with functions of clearing heat and detoxification. It has various pharmacological actions, including anti-inflammatory, antiviral, and antibacterial effects. Further research is needed for the comprehensive evaluation of its quality and understanding of multi-target network pharmacology.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kuei-Hung Lai, Yu-Li Chen, Mei-Fang Lin, Mohamed El-Shazly, Yu-Chia Chang, Po-Jen Chen, Chun-Han Su, Yen-Chun Chiu, Amina M. Illias, Chih-Chuan Chen, Lo-Yun Chen, Tsong-Long Hwang
Summary: The study found that the ethanol extract of Lonicerae japonicae flos has anti-inflammatory effects on human neutrophils activated by fMLF, suggesting its potential application in daily health care during the COVID-19 pandemic.
Article
Agriculture, Multidisciplinary
Zhengpeng Wang, Chao Jiang, Yan Jin, Jian Yang, Yuyang Zhao, Luqi Huang, Yuan Yuan
Summary: DNA methylation differences in the CpG islands upstream of genes in the chlorogenic acid and iridoid biosynthetic pathways are closely associated with the geographical origin of Lonicerae japonicae flos (LJF). Using cluster analysis and principal component analysis, LJFs from 39 geographical origins were classified into four groups: Northern China, Central Plain of China, Southeast China, and Western China. These findings provide insights into the modulation of LJF taste and the variation of DNA methylation in LJF with geographical origin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Lifei Gu, Xueqing Xie, Bing Wang, Yibao Jin, Lijun Wang, Guo Yin, Jue Wang, Kaishun Bi, Tiejie Wang
Summary: In this study, a chemical pattern recognition model was established based on ultra-high performance liquid chromatography (UHPLC) and anti-SARS-CoV2 activity to analyze the quality of Lonicerae japonicae flos and Lonicerae flos. A total of 11 characteristic components were identified and could be recommended as quality control markers. The established model effectively differentiated between Lonicerae japonicae flos and Lonicerae flos, providing a feasible tool for quality assessment of natural products and reference for quality standard setting.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kaishuang Liu, Yibao Jin, Lifei Gu, Meifang Li, Ping Wang, Guo Yin, Shuhong Wang, Tiejie Wang, Lijun Wang, Bing Wang
Summary: H-1-NMR spectroscopy combined with chemical pattern recognition was used to analyze samples of Lonicerae japonicae flos with different species, growth modes, and processing methods. Linear discriminant analysis (LDA) showed good classification abilities for distinguishing the samples based on species, growth modes, and processing methods. The study also identified the main differential components and explored the potential compounds for distinguishing cultivated and wild Lonicerae japonicae flos.
Article
Chemistry, Medicinal
Yu Gao, Feng-xue Wang, Qing Liu, Yao-dong Qi, Qiu-ling Wang, Hai-bo Liu
Summary: Lonicerae Japonicae Flos and Lonicerae Flos exhibit high similarity in treating inflammation, sharing many core targets and biological functions, making them interchangeable in clinical treatment. Organic acids are the main compounds responsible for their anti-inflammatory effects.
CHINESE HERBAL MEDICINES
(2021)
Article
Chemistry, Applied
Ran Yang, Lei Fang, Jia Li, Yong-Qing Zhang
Summary: A new lignan and three known compounds were isolated from Lonicerae Japonicae Flos, with one compound showing significant anti-inflammatory activity by inhibiting a specific release reaction.
NATURAL PRODUCT RESEARCH
(2021)
Article
Plant Sciences
Chang Liu, Zhigang Yin, Tingting Feng, Min Zhang, Zhi Zhou, Ying Zhou
Summary: This study identified the main active ingredients and potential targets of Lonicerae japonicae flos involved in protecting against ALI through network pharmacology and RNA-Seq analysis, providing directions for further research.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Zhichen Cai, Chengcheng Wang, Cuihua Chen, Lisi Zou, Chuan Chai, Jiali Chen, Mengxia Tan, Xunhong Liu
Summary: The study developed a comprehensive method using ultra-fast liquid chromatography-tandem triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS) coupled with multivariate statistical analysis for quality evaluation of Lonicerae Japonicae Flos and Lonicerae Flos, revealing differential components.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Plant Sciences
Wei Mu, Lan-hui Zhang, Wei Jiang, Tian Zhang, An Liu, Yong-qiang Zhang, Jun Zhao, Lei Shi, Lin-na Liu
Summary: Lonicerae japonicae flos (LJF) and its main component chlorogenic acid (CGA) have the potential to prevent vascular endothelial dysfunction induced by abdominal radiotherapy, possibly through increasing the expression of GTPCH1/BH4/eNOS signaling pathway.
Article
Integrative & Complementary Medicine
Pan Hui-Qin, Zhou Heng, Miao Shui, Guo De-An, Zhang Xiao-Li, Hu Qing, Mao Xiu-Hong, Ji Shen
Summary: The overuse and misuse of pesticides can lead to variations in herbal medicine ingredients, which has prompted attention in the medicinal field as an alternative medicine form. This study systematically explored the effects of two frequently used insecticides on the comprehensive constituents of Lonicerae Japonicae Flos, resulting in the identification of 29 metabolic markers.
CHINESE JOURNAL OF NATURAL MEDICINES
(2021)
Article
Biochemistry & Molecular Biology
Yang-Qiannan Tang, Li Li, Tian-Feng Lin, Li-Mei Lin, Ya-Mei Li, Bo-Hou Xia
Summary: In this study, a model for discriminating LJF and LF was established and applied in the discrimination of their related prescriptions. The results showed good accuracy and applicability, suggesting that this method is a rapid and effective tool for the successful discrimination of LJF and LF and their related prescriptions.
Article
Engineering, Multidisciplinary
Pengdi Cui, Yang Yu, Jing Zhao, Peiqi Miao, Qilong Xue, Changqing Liu, Zheng Li
Summary: Hyperspectral imaging was used to investigate the drying process of Lonicerae Japonicae Flos extract, providing information on the morphology and moisture content changes of droplets. The Faster R-CNN algorithm located and identified the target droplets, while the PLS and ANN algorithms established a moisture content prediction model, with the ANN showing better accuracy.
Article
Pharmacology & Pharmacy
Jing-Bei Zhang, Hong-Liu Jin, Xiao-Ying Feng, Sen-ling Feng, Wen-Ting Zhu, Hong-Mei Nan, Zhong-Wen Yuan
Summary: This study aims to explore the active components and epigenetic regulation mechanism underlying the anti-inflammatory effects of the herb-pair Lonicerae Japonicae Flos and Forsythiae Fructus (LFP) in carbon tetrachloride (CCl4)-induced rat liver fibrosis. The results suggest that LFP has a potential therapeutic effect on liver fibrosis by reducing collagen formation, inflammation, and oxidative stress.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Neurosciences
Ruifang Hua, Yan Ding, Xiaolong Liu, Bingxuan Niu, Xinfeng Chen, Jingjing Zhang, Kerui Liu, Pei Yang, Xiaofei Zhu, Jintao Xue, Hui Wang
Summary: Lonicerae Japonicae Flos (LJF) has anti-viral, anti-oxidative, and anti-inflammatory properties, and this study demonstrates its potential in treating sleep disorders related to sleep deprivation and neuro-inflammation.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)