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Synthetic fluorescent probes to map metallostasis and intracellular fate of zinc and copper

期刊

COORDINATION CHEMISTRY REVIEWS
卷 311, 期 -, 页码 125-167

出版社

ELSEVIER SCIENCE SA
DOI: 10.1016/j.ccr.2015.11.012

关键词

Cellular metal sensors; Metallome; Intracellular bioinorganic chemistry; Cu receptors; Zn receptors; Neuropathology; Confocal microscopy

资金

  1. MIUR (Italian Ministry for Research and University) grant FIRB MERIT [RBNE08HWLZ_001]

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The intracellular tracking of zinc and copper, metals essential for life, is nowadays pivotal to unravel the complex mechanism that involves the physiological or pathological role of such elements. Traditional methods to determine cellular copper and zinc levels, including those based on the use of fluorescent probes, are aimed at scrutinizing the metallome, to identify both the individual species and their concentrations. The metallome, however, is also a nonstatic concept, as it responds to environmental perturbations in biologically relevant pathways, with highly dynamic spatiotemporal changes. Through an overview of improvements and limits of the state of the art of synthetic fluorescent probes for the detection of intracellular zinc and copper, we report here new routes for the design and the synthesis of novel metal coordination compounds able to overcome the present weaknesses for a new concept of dynamic metallostasis. (C) 2015 Elsevier B.V. All rights reserved.

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