期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 432, 期 23, 页码 6168-6172出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2020.10.013
关键词
mass photometry; binding affinity; tubulin; single molecule
资金
- ERC [CoG 819593]
- Francis Crick Institute
- Cancer Research UK [FC001163]
- UK Medical Research Council [FC001163]
- Wellcome Trust [FC001163]
- European Research Council [323042]
- Spanish Ministry of Economy, Industry and Competitiveness
- Centro de Excelencia Severo Ochoa
- CERCA Programme of the Generalitat de Catalunya
- European Research Council (ERC) [323042] Funding Source: European Research Council (ERC)
The alpha beta-tubulin heterodimer is the fundamental building block of microtubules, making it central to several cellular processes. Despite the apparent simplicity of heterodimerisation, the associated energetics and kinetics remain disputed, largely due to experimental challenges associated with quantifying affinities in the 10(-2) s(-1). Our results demonstrate the capabilities of mass photometry for quantifying protein-protein interactions and clarify the energetics and kinetics of tubulin heterodimerisation. (C) 2020 The Author(s). Published by Elsevier Ltd.
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