Review
Cell Biology
Quyen Thu Bui, Jeong Hee Hong, Minseok Kwak, Ji Yeon Lee, Peter Chang-Whan Lee
Summary: The ubiquitin-mediated degradation system controls various tumor-promoting processes through the sequential action of E1, E2, and E3 enzymes, with E2 enzymes playing a central role in modulating pathophysiological processes in the tumor microenvironment.
Article
Biochemistry & Molecular Biology
Muthukumar Elangovan, Jun Ka, Boryeong Pak, Woosoung Choi, Se-Ra Oh, Suk-Won Jin, Yung Joon Yoo
Summary: Ubiquitination plays an important regulatory role in angiogenesis, with E3 enzymes being extensively studied. The role of E2 enzymes in angiogenesis is less known. Through analysis of single-cell RNA sequencing data, UBE2V1 was identified as one of the most abundantly expressed E2s in endothelial cells. Inhibition of UBE2V1 affects endothelial cell proliferation, viability, morphogenesis, and migration, and is critical for FGF2-induced angiogenesis.
Article
Fisheries
Jian Zhang, Xiangyi Sun, Xuepeng Li, Shuning Zhang, Xiudan Xu, Jixing Feng
Summary: Many viruses hijack the host's ubiquitin-proteasome system (UPS) for successful infection. This study identified and characterized a protein called SmE2D2 in turbot, which interacts with the RBIV-C1 virus. The results show that SmE2D2 is an E2 ubiquitin-conjugating enzyme that is used by RBIV-C1 to promote efficient replication.
Article
Biochemistry & Molecular Biology
Fumiyo Ikeda
Summary: The ubiquitin-proteasomal system and the autophagy-lysosome system are two major degradation systems in mammalian cells. Ubiquitin not only regulates proteasomal degradation of substrates but also regulates the autophagy pathway. Ubiquitin plays a key role in autophagy regulation by recruiting cargos to phagophore in a ubiquitin-dependent manner.
Article
Biochemistry & Molecular Biology
Derek L. Bolhuis, Raquel C. Martinez-Chacin, Kaeli A. Welsh, Tatyana Bodrug, Liying Cui, Michael J. Emanuele, Nicholas G. Brown
Summary: The proper degradation of proteins by the ubiquitin-proteasome system is crucial for the accurate progression of the cell cycle, and the activity of ubiquitin ligases is tightly regulated. This study investigated the role of the Anaphase-Promoting Complex/Cyclosome (APC/C) in mitotic progression and G1 maintenance, and discovered the involvement of EMI1 in regulating APC/C activity during G1. Additionally, it was found that a C-terminal peptide fragment of EMI1 can activate the priming of specific substrates by the APC/C.
Article
Biochemistry & Molecular Biology
Kazuhide Miyamoto, Atsushi Matsumoto
Summary: In this study, the unique properties of ARFs in E2-binding and auto-ubiquitination were demonstrated in ubiquitination assays. Point mutations of ARF by tryptophan resulted in changes in E2 specificity and Ub chain elongation. ARF mutants interacted with all 11 E2s and promoted auto-ubiquitination, allowing specific detection of E2 activities during ubiquitination.
Article
Plant Sciences
Pengcheng Wang, Kai Guo, Qian Su, Jinwu Deng, Xianlong Zhang, Lili Tu
Summary: The E2 enzyme GhUBC2L modulates histone monoubiquitination synergistically with E3 ligase GhUbox8 to control organ size in cotton by affecting the expression of genes related to cell cycle and organ development.
Article
Immunology
Jaime Larraga, Pedro J. Alcolea, Ana M. Alonso, Luis T. C. Martins, Inmaculada Moreno, Mercedes Dominguez, Vicente Larraga
Summary: Leishmania parasites cause significant morbidity and mortality in many developing countries. Researchers have identified a vaccine candidate, LiUBC1, which shows partial protection against L. infantum infection in a Syrian hamster model.
Review
Cell Biology
Xiu Yin, Qingbin Liu, Fen Liu, Xinchen Tian, Tinghao Yan, Jie Han, Shulong Jiang
Summary: This review summarizes the role of ubiquitination in tumorigenesis and related signaling pathways, aiming to provide a reference for future exploration of ubiquitination and potential targets for cancer therapies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Fengchao Zhang, Yao Chen, Jie Shen, Junzheng Zhang
Summary: The Notch signaling pathway plays crucial roles in animal development, with protein ubiquitination regulating the stability and activity of major signaling components. Multiple ubiquitin ligases and deubiquitinating enzymes have been identified in Drosophila that modify Notch ligand and receptor proteins. UbcD1 has been found to be required for Notch signaling activation during Drosophila wing development, suggesting a novel role for UbcD1 in development.
FRONTIERS IN GENETICS
(2021)
Article
Chemistry, Multidisciplinary
Lu-Jun Liang, Guo-Chao Chu, Qian Qu, Chong Zuo, Junxiong Mao, Qingyun Zheng, Jingnan Chen, Xianbin Meng, Yangwode Jing, Haiteng Deng, Yi-Ming Li, Lei Liu
Summary: This study describes a new total chemical synthesis approach for constructing a structurally new activity-based E2-Ub probe, which covalently captures the catalytic site of Cys-dependent E3 ligases. Chemical cross-linking coupled with mass spectrometry demonstrated the utility of this new probe in structural analysis of intermediates formed during thiol transfer processes. The study exemplifies the utility of chemical protein synthesis in developing protein probes for biological studies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemistry & Molecular Biology
Sachio Tsuchida, Tomohiro Nakayama
Summary: Oral health is crucial for overall well-being and may indicate systemic diseases, with the ubiquitin system playing a role in cellular functions and DNA repair. Deubiquitinating enzymes are essential regulators of ubiquitination-mediated degradation and have significant impacts on cellular pathways and biological processes. Research highlights the relationship between ubiquitination, deubiquitination, and oral diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jing-Yan Gao, Chih-Shiang Chang, Jin-Cherng Lien, Ting-Wei Chen, Jing-Lan Hu, Jing-Ru Weng
Summary: Trytanthrin, a natural compound found in Ban-Lan-Gen, has anti-inflammatory and anti-viral properties. Two derivatives, A1 and A6, showed higher cytotoxicity in HCC cells and targeted multiple aspects of cancer cell growth, making them potential candidates for clinical applications.
Article
Agronomy
Yunfei Han, Chuanzhong Zhang, Hanjing Sha, Xiaojing Wang, Yue Yu, Jia Liu, Guangxin Zhao, Jingying Wang, Guankai Qiu, Xingjian Xu, Jun Fang
Summary: In this study, a T-DNA insertional mutant named R164 with decreased root length was used to investigate the function of OsUBC11 in rice. It was found that OsUBC11 is involved in root development and modulates auxin signaling pathway, ultimately affecting root development at the rice seedling stage.
Article
Oncology
Jiguang Zhang, Jingdong Wang, Jincheng Wu, Jianyuan Huang, Zhaoxian Lin, Xing Lin
Summary: This study explored the role and mechanism of FANCI in non-small cell lung cancer (NSCLC). The results showed that FANCI expression was higher in NSCLC tumor tissues compared to adjacent tissues. Knockdown of FANCI inhibited cell proliferation, migration, invasion, cell cycle and epithelial-to-mesenchymal transition (EMT) in vitro. The study also found that FANCI was monoubiquitinated by UBE2T to regulate cell growth, migration and EMT through WDR48. These findings suggest that FANCI could be used as a prognostic biomarker and therapeutic target for NSCLC.
Article
Cell Biology
Ning Wang, Yaodong Jiang, Shidong Lv, Haoran Wen, Dehua Wu, Qiang Wei, Qiang Dang
Article
Oncology
Yanling Lin, Lingzhi Wang, Wenxiao Luo, Xiaohan Zhou, Yuting Chen, Kaifan Yang, Jinrong Liao, Dehua Wu, Longmei Cai
CANCER MANAGEMENT AND RESEARCH
(2020)
Article
Medicine, Research & Experimental
Zhibo Tan, Peitao Zhou, Zhenru Zhu, Ying Wang, Zeqin Guo, Mengying Shen, Yazhi Xiao, Weixi Shen, Dehua Wu
Summary: The study reveals that in esophageal squamous cell carcinoma (ESCC), long non-coding RNA LincIN is significantly overexpressed and exerts an oncogenic role through the regulation of the NF90/miR-7/HOXB13 axis. This suggests that LincIN may serve as a potential prognostic biomarker and therapeutic target for ESCC.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Xiaohan Zhou, Yanling Lin, Yuting Chen, Lingzhi Wang, Xiaohong Peng, Jinrong Liao, Hanyi Zeng, Wenxiao Luo, Dehua Wu, Longmei Cai
Summary: Research has shown that the upregulation of EBV-miR-BART8-3p in nasopharyngeal carcinoma patients is associated with radioresistance and metastasis. This miRNA promotes invasion and metastasis of NPC cells, suggesting it could be a potential therapeutic target.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yanling Lin, Xiaohan Zhou, Kaifan Yang, Yuting Chen, Lingzhi Wang, Wenxiao Luo, Yujiang Li, Jinrong Liao, Yingtong Zhou, Yiming Lei, Yanting Zhang, Dehua Wu, Longmei Cai
Summary: Radiotherapy is crucial for treating nasopharyngeal carcinoma (NPC), with PTPRD potentially enhancing radiation-induced autophagy through dephosphorylation of STAT3 to increase NPC sensitivity to radiation. Low PTPRD and high p-STAT3 levels may predict poor clinical outcomes in NPC patients.
Article
Oncology
Liting Zhong, Dehua Wu, Weiwei Peng, Hailong Sheng, Yazhi Xiao, Xuebing Zhang, Yuli Wang
Summary: The combination of PD-1/PD-L1 inhibitors with palliative radiotherapy and anti-angiogenic therapy appears to be safe and effective in treating hepatocellular carcinoma, with a significant impact on patient survival. Further studies are needed to explore the clinical benefits of this triple therapy approach.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Chengdong Liu, Xiaohan Zhou, Hanyi Zeng, Dehua Wu, Li Liu
Summary: The study found that HILPDA is highly expressed in most tumors and is associated with poorer overall survival, disease-specific survival, and progression-free interval. Additionally, HILPDA expression is significantly correlated with the glycolysis pathway and immune cell infiltration, suggesting a potential role in promoting TAM infiltration and tumor immunosuppression.
FRONTIERS IN ONCOLOGY
(2021)
Article
Immunology
Xiaohan Zhou, Jun Du, Chengdong Liu, Hanyi Zeng, Yuting Chen, Li Liu, Dehua Wu
Summary: CD161 is a potential cancer biomarker that shows differential expression and better survival prediction in most tumor types. It is closely correlated with immunoregulatory interactions between lymphoid and non-lymphoid cells, as well as with T cell infiltration, immune checkpoints, immune activating genes, immunosuppressive genes, chemokines, and chemokine receptors. These findings suggest that CD161 may synergize with other immune checkpoints to regulate the immune microenvironment, making it a potential target for new immunotherapy drugs development.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Yuan Fang, Yizhi Zhan, Yuwen Xie, Shisuo Du, Yuhan Chen, Zhaochong Zeng, Yaowei Zhang, Keli Chen, Yongjia Wang, Li Liang, Yi Ding, Dehua Wu
Summary: The study identified a link between radiation resistance and metabolic reprogramming in hepatocellular carcinoma (HCC). By targeting metabolic pathways, potential combination approaches for radiotherapy in HCC were suggested. Activation of glucose metabolism predicted poor response to radiotherapy in cancer patients.
Article
Cell Biology
Zhaoze Guo, Han Zhang, Yiming Fu, Junjie Kuang, Bei Zhao, LanFang Zhang, Jie Lin, Shuhui Lin, Dehua Wu, Guozhu Xie
Summary: Cancer-associated fibroblasts (CAFs) are the most common stromal cells in breast cancer, and they play a role in disease progression and chemoresistance. This study found that CAF-derived conditioned media can promote breast cancer cell growth and radioresistance. The secretion of interleukin 6 (IL-6) by CAFs activates the STAT3 signaling pathway, leading to the growth and radioresistance of breast cancer cells. Inhibition of STAT3 or neutralizing IL-6 can block the effects induced by CAFs. In mouse models, the IL-6 receptor monoclonal antibody tocilizumab can reverse CAF-induced growth and radioresistance. Additionally, poor response to radiotherapy in breast cancer is associated with the expression of IL-6 and p-STAT3. These findings highlight the importance of the IL-6/STAT3 signaling pathway as a potential therapeutic target in breast cancer radiotherapy.
CELL DEATH DISCOVERY
(2023)
Article
Oncology
Si-Cong Ma, Xin-Ran Tang, Li-Li Long, Xue Bai, Jian-Guo Zhou, Zhi-Jiao Duan, Jian Wang, Qiang John Fu, Hong-Bo Zhu, Xue-Jun Guo, Yan-Pei Zhang, Ze-Qin Guo, De-Hua Wu, Zhong-Yi Dong
Summary: The study revealed that the primary and metastatic lesion spectrum influence the efficacy of immune checkpoint blockade therapy in non-small cell lung cancer patients. Guidance based on the DSO model may offer effective medication choices for ICB treatment.
Article
Biochemistry & Molecular Biology
Chengdong Liu, Xiaohan Zhou, Qian Long, Hanyi Zeng, Qingcan Sun, Yuting Chen, Dehua Wu, Li Liu
Summary: Research has shown that miR-30a-3p is selectively sorted into HCC cells through sEVs, promoting cell migration and invasion when removed. HCC cells can efficiently absorb HSC sEVs, and treatment with HSC sEVs rich in miR-30a-3p cargo effectively attenuates HCC migration, invasion, and metastasis.
Article
Oncology
He-San Luo, Shao-Fu Huang, Hong-Yao Xu, Xu-Yuan Li, Sheng-Xi Wu, De-Hua Wu
RADIATION ONCOLOGY
(2020)
Article
Oncology
Ping-Yan Liao, Zhong-Yi Dong, Chan-Tao Huang, Xin-Ran Tang, Guan-Dong Liu, Zhu-Liu, De-Hua Wu
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
(2020)