Article
Cell Biology
Ling Liu, Meixiong Cheng, Tian Zhang, Yong Chen, Yaqiu Wu, Qi Wang
Summary: The regulatory mechanism of MSC-derived EV-miR-744-5p in glioma was explored in this study. It was found that MSC-EVs-miR-744-5p can downregulate TGFB1, suppress the MAPK signaling activity, and inhibit M2 polarization of macrophages, thereby preventing glioma progression.
CELL BIOLOGY AND TOXICOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Kanglu Li, Guohua Yan, Hanji Huang, Mingjun Zheng, Ke Ma, Xiaofei Cui, Dejie Lu, Li Zheng, Bo Zhu, Jianwen Cheng, Jinmin Zhao
Summary: This study investigates the immunomodulatory and anti-inflammatory effects of human umbilical cord mesenchymal stem cells-derived extracellular vesicles (hUCMSCs-EVs) on osteoarthritis (OA). The results suggest that hUCMSCs-EVs promote the polarization of M2-type macrophages and the expression of IL-10, inhibit inflammation-associated factors in OA chondrocytes caused by IL-1 beta, and alleviate cartilage degradation during OA progression. The enriched miRNAs in hUCMSCs-EVs and the modulation of the PI3K-Akt signaling pathway may contribute to these therapeutic effects.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Oncology
Yi Cao, Yi Tu, Jianbo Xiong, Shengxing Tan, Lianghua Luo, Ahao Wu, Xufeng Shu, Zhigang Jie, Zhengrong Li
Summary: This study found that miR-15b-5p transferred by M2 macrophage-derived EVs plays an important role in the metastasis of gastric cancer. miR-15b-5p promotes the proliferative and invasive potentials, as well as epithelial-mesenchymal transition (EMT), of GC cells by targeting BRMS1. BRMS1, in turn, suppresses the expression of DAPK1, leading to inhibition of GC cell metastasis. In vivo experiments demonstrated that overexpression of miR-15b-5p in GC cells enhances tumor metastasis. Therefore, miR-15b-5p may serve as a therapeutic target for the treatment of GC metastasis.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Immunology
Ji Wu, Yao Liao, Dinghao Li, Zifeng Zhu, Lichao Zhang, Zhongdao Wu, Ping He, Lifu Wang
Summary: This study found that Trichinella-derived extracellular vesicles and their miR-1-3p and let-7-5p can regulate the immune response and fibroblasts of the host, promoting the polarization of macrophages while inhibiting fibroblast activation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Wenli Zhou, Shiquan Li, Xiaona Zhang, Chao Li, Jiantao Zhang
Summary: Extracellular vesicles (EVs) containing miR-143-3p derived from M2 macrophages play a crucial role in colorectal cancer by promoting aggressive tumor biology, tumor growth, and epithelial-mesenchymal transition. MiR-143-3p inhibits the expression of ZC3H12A gene and increases the expression of C/EBP beta, contributing to the development of CRC.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Oncology
Yuanshan Yao, Chunji Chen, Jing Wang, Haojie Xuan, Xiuxiu Chen, Zheng Li, Fuzhi Yang, Bin Wang, Siyun Lin, Saitian Li, Dongfang Tang, Libao Gong, Wen Gao
Summary: Our study reveals the functional role of circATP9A in NSCLC. We have demonstrated that circATP9A can promote the progression of NSCLC through in vivo and in vitro experiments. Mechanistically, circATP9A forms an RNA-protein complex with HuR protein, thereby amplifying the expression of the target gene NUCKS1. Furthermore, the PI3K/AKT/mTOR signaling pathway is identified as the downstream pathway of circATP9A/HuR/NUCKS1 axis. It is also found that hnRNPA2B1 mediates the incorporation of circATP9A into extracellular vesicles (EVs), which induce the M2 polarization of tumor-associated macrophages (TAMs) and facilitate NSCLC development.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Cell Biology
Makon-Sebastien Njock, Tina O'Grady, Olivier Nivelles, Michelle Lion, Sophie Jacques, Maureen Cambier, Stephanie Herkenne, Florian Muller, Aurelie Christian, Claire Remacle, Julien Guiot, Souad Rahmouni, Franck Dequiedt, Ingrid Struman
Summary: This study found that endothelial cell-derived extracellular vesicles (EVs) participate in the recruitment of macrophages within the tumour in the tumour microenvironment, leading to a change in the immunomodulatory phenotype and promoting tumour growth.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biochemistry & Molecular Biology
Uliana Dyachkova, Maksim Vigovskiy, Nataliya Basalova, Anastasia Efimenko, Olga Grigorieva
Summary: Fibrosis is a significant contributor to mortality rate in developed countries, and multipotent mesenchymal stromal cells (MSC) can suppress its development. However, age-related chronic inflammation can induce MSC senescence and impair their antifibrotic properties. This study investigates the effects of alternatively activated macrophages (M2-type) and hydrogen peroxide (H2O2) on MSC senescence and fibroblast-to-myofibroblast differentiation. The results demonstrate that M2-macrophage-induced chronic inflammation and H2O2 exposure can induce MSC senescence and decrease their ability to inhibit fibroblast-to-myofibroblast differentiation. However, subsequent cultivation can reverse the senescence and restore the MSC's antifibrotic functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Cansu Gorgun, Chiara Africano, Maria Chiara Ciferri, Nadia Bertola, Daniele Reverberi, Rodolfo Quarto, Silvia Ravera, Roberta Tasso
Summary: This study demonstrates that EVs derived from MSCs preconditioned with hypoxia inflammatory cytokines can attenuate inflammaging and alleviate age-related inflammation and mitochondrial dysfunction.
Review
Cell Biology
Theodore Reed, Jeffrey Schorey, Crislyn D'Souza-Schorey
Summary: Extracellular vesicles are a diverse population of membrane-bound particles that can induce various effects on recipient cells. In the tumor microenvironment, EVs released by tumor cells play a crucial role in influencing the functional plasticity of macrophages in tumor progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Na Li, Zhiqi Gao, Lu Zhao, Bei Du, Binyun Ma, Hong Nian, Ruihua Wei
Summary: This study demonstrates that small extracellular vesicles derived from human umbilical cord mesenchymal stem cells can prevent and treat autoimmune dry eye by promoting macrophage polarization and Treg generation. The enrichment of miR-100-5p in these vesicles plays a functional role in their immunomodulatory effects.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Thu Huyen Nguyen, Huy Hoang Dao, Chau Minh Duong, Xuan-Hung Nguyen, Diem Huong Hoang, Xuan-Hai Do, Trung Quang Truong, Tu Dac Nguyen, Liem Thanh Nguyen, Uyen Thi Trang Than
Summary: In recent years, extracellular vesicles (EVs) secreted by mesenchymal stem cells (MSCs) have shown potential as a cell-free therapy for osteoarthritis (OA). This study investigated the effects of EVs released by cytokine-primed umbilical cord-derived MSCs (UCMSCs) on osteoarthritic chondrocyte physiology. The results showed that EVs from cytokine-primed UCMSCs could enhance chondrocyte migration and potentially induce chondrocyte regeneration. These findings suggest that cytokine priming of UCMSCs can selectively deliver therapeutic effects of EV treatment in OA and chondrocyte-related disorders.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Environmental Sciences
Yifei Qin, Jing Zhang, Ricardo David Avellan-Llaguno, Xu Zhang, Qiansheng Huang
Summary: DEHP exposure promotes the secretion of miR-26a-5p in sEV, subsequently enhancing the EMT, migration, and invasion ability in neighboring normal cells.
ENVIRONMENTAL POLLUTION
(2021)
Article
Pharmacology & Pharmacy
Kristeen Ye Wen Teo, Shipin Zhang, Jia Tong Loh, Ruenn Chai Lai, Hwee Weng Dennis Hey, Kong-Peng Lam, Sai Kiang Lim, Wei Seong Toh
Summary: MSC exosomes promote the polarization of M2-like macrophages through activation of the toll-like receptor signaling pathway and CD73-mediated adenosine production. The production of adenosine activates AKT/ERK-dependent signaling pathways via binding to adenosine receptors A(2A) and A(2B). These findings highlight the critical role of CD73 in mediating M2-like macrophage polarization by MSC exosomes, and have implications for predicting the immunomodulatory potency of MSC exosome preparations.
Article
Cell Biology
Jian Chang, Hanjun Li, Zhongchao Zhu, Pei Mei, Weimin Hu, Xingcheng Xiong, Jing Tao
Summary: This research demonstrates that M2 macrophage-derived exosomal miR-21-5p promotes differentiation and activity of PaCa stem cells by targeting KLF3. downregulation of miR-21-5p in M2 macrophage-derived EVs inhibits key cancer stem cell functions in vitro and tumor-forming ability in vivo.
CELL BIOLOGY AND TOXICOLOGY
(2022)