Review
Cell Biology
Daniel M. Davies, Kim van den Handel, Soham Bharadwaj, Jette Lengefeld
Summary: Years of research have shown that controlling cell size is crucial for cells, and accurate size control is important for aging. Recent studies indicate that cellular enlargement is connected to aging and age-related diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Hematology
Laura R. Goldberg
Summary: Extracellular vesicles (EVs) play a critical role in modulating hematopoiesis within the bone marrow microenvironment and may be key mediators of HSC aging. Studies have shown their involvement in numerous age-related biologic processes and diseases, influencing both normal and malignant hematopoiesis.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Claudia Morganti, Keisuke Ito
Summary: Mitochondrial dysfunction and stem cell exhaustion are characteristic features of aging, particularly in the hematopoietic system. Understanding the key mechanisms of HSC aging, including mitochondrial ROS production and alterations in metabolism, could lead to the identification of new therapeutic targets to prevent, delay, or reverse aspects of the aging process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Sarah Bouali, Roxann Hetu-Arbour, Celia Gardet, Krista M. Heinonen
Summary: Vangl2 is a crucial regulator in the self-renewal and differentiation of HSCs. Deletion of Vangl2 impairs HSC expansion and hematopoietic recovery, indicating its essential role in hematopoiesis regulation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Takeshi Fujino, Shuhei Asada, Susumu Goyama, Toshio Kitamura
Summary: This review discusses the causes of hematopoietic stem cell aging, including both intrinsic and extrinsic factors. Epigenetics and inflammation have been found to be involved in the connection between HSC aging, clonality, and oncogenesis. Clarifying the mechanisms of HSC aging has accelerated the development of therapeutic interventions for rejuvenating HSCs.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Cell Biology
Geovanny Genaro Reivan Ortiz, Yasaman Mohammadi, Ahmad Nazari, Mehrnaz Ataeinaeini, Parisa Kazemi, Saman Yasamineh, Bashar Zuhair Talib Al-Naqeeb, Haider Kamil Zaidan, Omid Gholizadeh
Summary: Aging is a biological process that leads to a decrease in the organism's standard of living due to time-related cellular and functional impairments. Recent advances in aging investigation have revealed that the rate of senescence can be regulated through genetic pathways and biological processes. Hematopoietic stem cells (HSCs) maintain blood generation throughout an organism's lifetime but are also affected by the senescence process, resulting in a decline in their capabilities. MicroRNAs (miRNAs) play a crucial role in controlling HSCs and can modulate senescence and age-associated diseases.
CELL COMMUNICATION AND SIGNALING
(2023)
Review
Immunology
Yiouli P. Ktena, Margarita Dionysiou, Lukasz P. Gondek, Kenneth R. Cooke
Summary: Epigenetics studies the regulation of gene expression without altering the DNA sequence. It plays a vital role in cellular homeostasis, differentiation, hematopoiesis, and immunity. Epigenetic modifications can be heritable and reversible, and there is growing interest in their potential role in allogeneic hematopoietic transplantation. This review provides an overview of epigenetic modifications and their functions, focusing on hematopoiesis and immunity in the context of allogeneic hematopoietic stem cell transplantation.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Cell Biology
Ying Lyu, Yejing Ge
Summary: Skin, the largest organ in the human body, undergoes aging processes such as wrinkling and hair graying, which are associated with epigenetic and metabolic deregulations. In the skin epithelium, the epigenome and metabolome play important roles in shaping cell lineage plasticity and regulating stem cell function during aging. This review summarizes recent studies on the molecular mechanisms of hair follicle degeneration, comparing findings from skin with model organisms and adult stem cells from other tissues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Ram Vinay Pandey, Johanna Strobl, Anna Redl, Luisa Unterluggauer, Laura Gail, Lisa Kleissl, Sophie Mueller, Denise Atzmueller, Victoria Fife-Gernedl, Thomas Krausgruber, Hanna Knaus, Margit Mitterbauer, Philipp Wohlfarth, Werner Rabitsch, Christoph Bock, Georg Stary
Summary: Allogeneic hematopoietic stem-cell transplantation (HSCT) aims to rebuild the host's immune system using donor stem cells. However, there are complications that can affect the success of HSCT, such as leukemia relapse or graft-versus-host-disease (GvHD). This study investigated the regulatory processes involved in T cell recovery after HSCT, using multi-omics analysis of T cells from the skin and blood. The findings revealed the importance of epigenetic regulation and identified specific epigenetic regulators in restoring T cell homeostasis after transplantation.
CLINICAL IMMUNOLOGY
(2023)
Review
Immunology
Guancui Yang, Xiang Wang, Shiqin Huang, Ruihao Huang, Jin Wei, Xiaoqi Wang, Xi Zhang
Summary: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the only curative treatment for myeloid malignancies, but relapse and graft-versus-host disease (GvHD) still impact patient survival. Epigenetic agents, such as hypomethylating agents (HMAs), have been explored to reduce relapse risk and prevent GvHD, and combining epigenetic therapy with HSCT may optimize the transplantation process and prevent treatment failure.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Frauke Gotzhein, Tim Aranyossy, Lars Thielecke, Tanja Sonntag, Vanessa Thaden, Boris Fehse, Ingo Mueller, Ingmar Glauche, Kerstin Cornils
Summary: In this study, a murine model of HSCT was used to investigate the contribution of different hematological subpopulations to the reconstitution process and the impact of age on this process. The results showed that hematopoietic stem cells and multipotent progenitors played a major role in reconstitution, independent of age. The reconstitution patterns were stable in all age groups. These findings support the expansion of HSCT in older individuals as a promising strategy for treating hematological diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Govinda Bhattarai, Hyun-Jaung Sim, Han-Sol So, Jeong-Chae Lee, Sung-Ho Kook
Summary: Research has mainly focused on the negative impacts of PM2.5 on respiratory, brain, immune, and metabolic diseases, with little knowledge about its effects on hematopoietic stem cell (HSC) fate. This study investigated the effects of atmospherically relevant PM2.5 on newborn hematopoietic stem progenitor cells (HSPCs), revealing increased oxidative stress and inflammasome activation in the lungs and bone marrow of PM2.5-exposed mice.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Shirly Becker-Herman, Milena Rozenberg, Carmit Hillel-Karniel, Naama Gil-Yarom, Mattias Kramer, Avital Barak, Lital Sever, Keren David, Lihi Radomir, Hadas Lewinsky, Michal Levi, Gilgi Friedlander, Richard Bucala, Amnon Peled, Idit Shachar
Summary: This study reveals that mice lacking CD74 exhibit accumulation of HSCs in the bone marrow, suggesting that CD74 may regulate the maintenance of HSCs and CD18 expression. Blocking CD74 also increases the numbers of HSPCs, which could improve transplant protocols.
Article
Biology
Leonard Herault, Mathilde Poplineau, Adrien Mazuel, Nadine Platet, Elisabeth Remy, Estelle Duprez
Summary: By analyzing 15,000 young and aged HSC transcriptomes, the study identified 15 groups of HSCs showing rare and new specific abilities that change with age. This established a new reference map of HSC differentiation and revealed a potential mechanism that delays differentiation in aged HSCs, potentially contributing to age-related hematologic diseases.
Review
Plant Sciences
Eun-Deok Kim, Keiko U. Torii
Summary: The formation of stomata is a significant model system for understanding stem cell fate commitment. The regulatory strategies governing cell fate commitment differ between embryonic and postembryonic stomatal development. The interplay of transcription factors and cell cycle machineries is crucial for cell differentiation. Recent studies have provided insights into the molecular mechanisms underlying the interaction between transcription factors and epigenetic machineries.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Hassen Kared, Shu Wen Tan, Mai Chan Lau, Marion Chevrier, Crystal Tan, Wilson How, Glenn Wong, Marie Strickland, Benoit Malleret, Amanda Amoah, Karolina Pilipow, Veronica Zanon, Naomi Mc Govern, Josephine Lum, Jin Miao Chen, Bernett Lee, Maria Carolina Florian, Hartmut Geiger, Florent Ginhoux, Ezequiel Ruiz-Mateos, Tamas Fulop, Reena Rajasuriar, Adeeba Kamarulzaman, Tze Pin Ng, Enrico Lugli, Anis Larbi
NATURE COMMUNICATIONS
(2020)
Review
Oncology
Polina Zjablovskaja, Maria Carolina Florian
Article
Cell Biology
Maria Carolina Florian, Hanna Leins, Michael Gobs, Yang Han, Gina Marka, Karin Soller, Angelika Vollmer, Vadim Sakk, Kalpana J. Nattamai, Ahmad Rayes, Xueheng Zhao, Kenneth Setchell, Medhanie Mulaw, Wolfgang Wagner, Yi Zheng, Hartmut Geiger
Article
Oncology
Sachin Kumar, Kalpana J. Nattamai, Aishlin Hassan, Amanda Amoah, Rebekah Karns, Cuiping Zhang, Ying Liang, Akiko Shimamura, M. Carolina Florian, Ute Bissels, Martha Luevano, Andreas Bosio, Stella M. Davies, Medhanie Mulaw, Hartmut Geiger, Kasiani C. Myers
Summary: The research discovered that SDS patients have lower Cdc42 activity in their HSCs, and the extent of HSC depletion is related to the level of apolarity in HSCs. Exogenously provided Wnt5a or GDF11 can restore polarity in SDS HSCs and increase the number of HSCs.
Article
Hematology
Amanda Amoah, Anja Keller, Ramiz Emini, Markus Hoenicka, Andreas Liebold, Angelika Vollmer, Karina Eiwen, Karin Soller, Vadim Sakk, Yi Zheng, Maria Carolina Florian, Hartmut Geiger
Summary: This study characterizes age-related phenotypes of human hematopoietic stem cells (HSC). It reveals increased frequencies of HSC, hematopoietic progenitor cells, and lineage negative cells in the elderly, as well as a decreased frequency of multi-lymphoid progenitors. The elevated activity of Cdc42 in aged HSC contributes to age-related changes and offers a potential target for attenuating aging in human HSC.
Article
Cell & Tissue Engineering
Ani Grigoryan, Johannes Pospiech, Stephen Kramer, Daniel Lipka, Thomas Liehr, Hartmut Geiger, Hiroshi Kimura, Medhanie A. Mulaw, Maria Carolina Florian
Summary: The study demonstrates the important role of LaminA/C in establishing a repressive compartment for XCI in young HSCs, which becomes impaired in aged HSCs, leading to dysregulation of XCI.
Editorial Material
Hematology
Francesca Matteini, M. Carolina Florian
Summary: This article describes the role of the microbiome-IL1 axis as a key, self-sustaining, but reversible driver of hematopoietic stem cell inflammaging.
Article
Biochemistry & Molecular Biology
Ravinder Kandi, Katharina Senger, Ani Grigoryan, Karin Soller, Vadim Sakk, Tanja Schuster, Karina Eiwen, Manoj B. Menon, Matthias Gaestel, Yi Zheng, Maria Carolina Florian, Hartmut Geiger
Summary: The Cdc42-Borg4-Septin7 axis is crucial for maintaining polarity and function in HSCs, with genetic deletion of Borg4 or Septin7 resulting in reduced polarity frequency and engraftment potential of HSCs.
Review
Immunology
Francesca Matteini, Medhanie A. Mulaw, M. Carolina Florian
Summary: The aging of the bone marrow niche leads to declining HSC function, but certain niche structures and signals are crucial for maintaining HSC function. The use of new technical tools has revealed the impact of BM niche aging on HSCs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Ruzhica Bogeska, Ana-Matea Mikecin, Paul Kaschutnig, Malak Fawaz, Marleen Buechler-Schaeff, Duy Le, Miguel Ganuza, Angelika Vollmer, Stella Paffenholz, Noboru Asada, Esther Rodriguez-Correa, Felix Frauhammer, Florian Buettner, Melanie Ball, Julia Knoch, Sina Staeble, Dagmar Walter, Amelie Petri, Martha J. Carreno-Gonzalez, Vinona Wagner, Benedikt Brors, Simon Haas, Daniel B. Lipka, Marieke A. G. Essers, Vivienn Weru, Tim Holland-Letz, Jan-Philipp Mallm, Karsten Rippe, Stephan Kraemer, Matthias Schlesner, Shannon McKinney Freeman, Maria Carolina Florian, Katherine Y. King, Paul S. Frenette, Michael A. Rieger, Michael D. Milsom
Summary: Infection or inflammation may lead to irreversible depletion of functional hematopoietic stem cells, resulting in long-term effects on tissue maintenance and regeneration.
Editorial Material
Biochemistry & Molecular Biology
Rebecca Andersson, Maria Carolina Florian
Summary: This study provides the first comprehensive characterization of the haematopoietic system of naked mole-rats and reveals potential strategies to sustain haematopoiesis during an extended lifetime.
Article
Cell & Tissue Engineering
Sara Montserrat-Vazquez, Noelle J. Ali, Francesca Matteini, Javier Lozano, Tu Zhaowei, Eva Mejia-Ramirez, Gina Marka, Angelika Vollmer, Karin Soller, Mehmet Sacma, Vadim Sakk, Loris Mularoni, Jan Philipp Mallm, Mireya Plass, Yi Zheng, Hartmut Geiger, M. Carolina Florian
Summary: This study demonstrates that a short systemic treatment to decrease the activity of small RhoGTPase Cdc42 can improve the regenerative capacity of aged stem cells. Transplanting these rejuvenated stem cells can extend the healthspan and lifespan of aged mice, showing a broad systemic effect.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Biochemical Research Methods
Mehmet Sacma, Francesca Matteini, Medhanie A. Mulaw, Ali Hageb, Ruzhica Bogeska, Vadim Sakk, Angelika Vollmer, Gina Marka, Karin Soller, Michael D. Milsom, Maria Carolina Florian, Hartmut Geiger
Summary: This study proposes a gentle but fast whole-mount immunofluorescence staining protocol that preserves the 3D structure of complex interactions between stem cells and their microenvironment. The protocol enables high-resolution 3D imaging of various mouse organs and tissues.
Article
Cell & Tissue Engineering
Novella Guidi, Gina Marka, Vadim Sakk, Yi Zheng, Maria Carolina Florian, Hartmut Geiger
Summary: The study found that an aged niche restrains the function of ex vivo rejuvenated old HSCs, with a low level of the cytokine osteopontin being a contributing factor. Sustainable rejuvenation of the function of aged HSCs in vivo will need to address the influence of an aged niche on rejuvenated HSCs.
