Article
Oncology
Richard J. Sove, Babita K. Verma, Hanwen Wang, Won Jin Ho, Mark Yarchoan, Aleksander S. Popel
Summary: This study uses quantitative systems pharmacology (QSP) framework to conduct a virtual clinical trial for nivolumab and ipilimumab in HCC patients. The model incorporates detailed biological mechanisms of immune cell-cancer cell interactions and generates virtual patients for the trial. The predictions of the model are consistent with clinically observed outcomes, demonstrating the potential of QSP models in patient selection and trial design.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Devalingam Mahalingam, Leonidas Chelis, Imran Nizamuddin, Sunyoung S. Lee, Stylianos Kakolyris, Glenn Halff, Ken Washburn, Kristopher Attwood, Ibnshamsah Fahad, Julia Grigorieva, Senait Asmellash, Krista Meyer, Carlos Oliveira, Heinrich Roder, Joanna Roder, Renuka Iyer
Summary: This study utilizes a machine learning algorithm along with mass spectral data and AFP values from serum samples to detect HCC with high sensitivity and specificity. The results suggest that this method can improve cancer detection, especially for small lesions.
Article
Chemistry, Applied
Isac H. Cordeiro, Nerilson M. Lima, Elaine C. Scherrer, Gabriela P. Carli, Teresinha de Jesus A. S. Andrade, Sandra B. R. Castro, Marcone A. L. de Oliveira, Caio Cesar S. Alves, Alessandra P. Carli
Summary: This study extracted and identified protease inhibitor from Alocasia macrorrhizos and examined its immunomodulatory properties and cell viability. The results showed two bands with 11 and 24 kDa through SDS-PAGE analysis, and intense ions of m/z 4276.795 and 8563.361 in the roasted protein extract through MS analysis. The protease inhibitors from roasted tubers reduced the production of NO and TNF-α without affecting cell viability at concentrations below 100 µg mL(-1).
NATURAL PRODUCT RESEARCH
(2023)
Review
Immunology
Charlotte Durand, Daniele Maubon, Muriel Cornet, Yan Wang, Delphine Aldebert, Cecile Garnaud
Summary: Systemic antifungal agents are important for the treatment of invasive fungal infections, but the emergence of resistant strains is a concern. Antifungal susceptibility testing is crucial for patient management, but current methods have limitations such as high turnaround time. Innovative methods like MALDI-TOF and flow cytometry are promising alternatives, with the potential to improve the speed and accuracy of testing in clinical laboratories.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Tomoya Yoshinari, Aoi Sekine, Naoki Kobayashi, Yuzo Nishizaki, Naoki Sugimoto, Yukiko Hara-Kudo, Maiko Watanabe
Summary: This study used SDS-PAGE and MALDI-TOF MS to analyze the sources of 36 enzyme preparations (EPs), and found that 30 EPs had consistent sources with the manufacturer's information, 5 EPs showed high sequence similarity to closely-related species, and 6 enzymes could not be identified. This approach can rapidly determine the biological origin of enzymes and contribute to ensuring the safety of EPs.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Infectious Diseases
Hanene Benyahia, Adama Zan Diarra, Djalel Eddine Gherissi, Jean-Michel Berenger, Ahmed Benakhla, Philippe Parola
Summary: This study identified ticks collected from turtles in northeastern Algeria and found that these ticks carry various microorganisms associated with diseases. The research confirmed the reliability of MALDI-TOF MS as a tool for tick identification.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Immunology
Haoyang Mi, Won Jin Ho, Mark Yarchoan, Aleksander S. Popel
Summary: This study used fluorescent cell mapping technology to analyze the tumor microenvironment in patients with HCC, and found that the expression patterns of PD-L1 and Granzyme B were different in different regions. The close contact between CD8(+) T cells and Arg1(hi) macrophages was a prominent feature in non-responders. In addition, cell community analysis found that the communication between macrophage-enriched cell communities and lymphocyte-enriched cell communities with tumor cell communities was stronger in non-responders.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Computer Science, Interdisciplinary Applications
Chungang Zhuang, Zhenhua Xiong, Han Ding
Summary: This paper presents a computationally efficient MATLAB implementation of 2D/3D topology optimization in non-uniform rational basis spline (NURBS) framework. By utilizing page-wise matrix operations and graphics processing unit (GPU), the computational efficiency is greatly improved. The advantages of page-wise matrix operations are demonstrated through performance evaluation of benchmark cases.
STRUCTURAL AND MULTIDISCIPLINARY OPTIMIZATION
(2023)
Article
Biology
Zsolt Kotroczo, Istvan Fekete, Katalin Juhos, Nandor Prettl, Priyo Adi Nugroho, Gabor Varbiro, Borbala Biro, Tamas Kocsis
Summary: Global climate change affects soil decomposition processes through changes in temperature, precipitation, and biomass production, leading to alterations in soil organic matter and carbon stocks. Our research examines the effects of litter input changes on decomposition processes and soil carbon storage capacity, using various soil-biological indicators. The results highlight the importance of specific organic matter for enhancing soil biological activity and carbon content.
Article
Microbiology
Antoine Huguenin, Bernard Pesson, Matthieu L. Kaltenbach, Adama Zan Diarra, Philippe Parola, Jerome Depaquit, Fano Jose Randrianambinintsoa
Summary: Leishmania infantum is the agent of visceral leishmaniasis in the Mediterranean basin, transmitted by sandflies of the subgenus Larroussius. MALDI-TOF MS is a promising tool for rapid and accurate identification of field-caught sandflies, with the application of machine learning to distinguish similar species.
Article
Oncology
Xu Wang, Tao Zhou, Xingyi Chen, Yu Wang, Yushi Ding, Haoyang Tu, Shengyang Gao, Haoyu Wang, Xinying Tang, Yong Yang
Summary: Our study identifies ZNF207 as an immunosuppressive target in HCC, promoting HCC progression by regulating CD8(+) T cell infiltration and exhaustion. Mechanistically, ZNF207 is involved in the MAPK-chemokine C-X3-C-motif ligand axis and the regulation of indoleamine 2,3-dioxygenase 1 levels, leading to CD8(+) T cell exhaustion. Silencing ZNF207 could be beneficial for anti-PD1 combination therapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Chemistry, Analytical
Patricia Sousa, Irene Camacho, Jose S. Camara, Rosa Perestrelo
Summary: Researchers have developed a potential diagnostic method for breast cancer using SDS-PAGE and MALDI-TOF MS, which can distinguish between breast cancer patients and healthy individuals based on peptide ion biosignatures.
Article
Gastroenterology & Hepatology
Cheng Zhang, Shan Wu, Xiao-Dong Yang, Hui Xu, Tai Ma, Qi-Xing Zhu
Summary: This study identified 93 commonly upregulated genes and 36 commonly downregulated genes in HDV-HCC. The top 5 upregulated hub genes were TFRC, ACTR2, ARPC1A, ARPC3, and ARPC2, while the top 5 downregulated hub genes were CTNNB1, CCND1, CDKN1B, CDK4, and CDKN1A. Validation analysis showed that ARPC1A, ARPC3, and CDK4 were promoted in general liver cancer samples, and higher expressions of ARPC2 and CDK4 and lower expressions of CDKN1A, CCND1, and CDKN1B were associated with worse prognosis in general HCC patients.
TURKISH JOURNAL OF GASTROENTEROLOGY
(2021)