Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Biophysics
Man Zhao, Xiaolin Yao, Jiawei Li, Hulan Hu, Jing Ren, Jingke Xu, Jianlong Wang, Daohong Zhang
Summary: In this study, a moderate and convenient approach for the determination of staphylococcus aureus (S. aureus) was developed using organic-inorganic hybrid nanoflowers and Pig IgG in an enzyme-linked immunosorbent assay (ELISA) system. The synthesized VAN-HRP-CaHPO4 nanoflowers showed the ability to specifically capture gram-positive bacteria and enhance stability and enzymatic activity for signal amplification. This ELISA system successfully detected S. aureus with a detection limit of 4.3 CFU mL-1, outperforming some commercial ELISA kits. The proposed assay also demonstrated acceptable recovery in detecting S. aureus in food samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Nanoscience & Nanotechnology
Jiajia Huang, Lei Jiao, Weiqing Xu, Qie Fang, Hengjia Wang, Xiaoli Cai, Hongye Yan, Wenling Gu, Chengzhou Zhu
Summary: Enzyme immobilization is crucial for addressing the fragility of enzymes, but it may impact their bioactivity and catalytic efficiency. PdCu hydrogel nanozymes were used to immobilize horseradish peroxidase, resulting in improved stability and catalytic activity for biosensing of carcinoembryonic antigen. This approach showed promising potential for clinical diagnosis with significantly enhanced detection sensitivity.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Tingting Yan, Jiang Zhu, Ying Li, Ting He, Yunhuang Yang, Maili Liu
Summary: Aflatoxin B-1 (AFB(1)) contamination poses a significant threat to food safety and human health, requiring continuous monitoring. In this study, a biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) was developed for sensitive and rapid detection of AFB(1) in cereal. The BA-ELISA demonstrated improved sensitivity, reduced assay time, and significant antibody savings compared to conventional ELISA.
Article
Food Science & Technology
Lin Wei, Deyan Xu, Bei Yuan, Chengchen Pang, Haitao Xu, Kunying Nie, Qingqing Yang, Sibel A. Ozkan, Yanyan Zhang, Yemin Guo, Xia Sun
Summary: This study successfully developed a new MBs-icELISA method for detecting AFB(1) with high sensitivity and linear correlation, significantly reducing the detection time compared to conventional ELISA methods.
Article
Chemistry, Multidisciplinary
Jianlei Shen, Bo Situ, Xianchao Du, Zhiming Wang, Rong Hu, Baixue Li, Anjun Qin, Ben Zhong Tang
Summary: This study presents a strategy to improve the detection sensitivity and broaden the dynamic range of enzyme-linked immunosorbent assay (ELISA) by replacing traditional chromogenic reagents with an aggregation-induced emission luminogen (AIEgen). The developed AIE-ELISA showed a lower limit of detection and a broader dynamic range compared to single-mode ELISA in the detection of prostate-specific antigen (PSA), allowing for differentiation of minor PSA differences in patient's serum. The AIE-ELISA demonstrated simpler experimental operation, faster enzyme response speed, and better photostability than traditional chromogenic reagents, making it highly promising in the fields of immunoassay, immunohistochemistry, and immunocytochemistry.
Article
Food Science & Technology
Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: This study developed a sandwich enzyme-linked immunosorbent assay (ELISA) for highly selective and ultra-sensitive quantitative analysis of selenium (Se) in food samples. The approach showed a remarkable limit of detection (LOD) at the femtomolar level and a substantial enhancement in sensitivity. The method was validated by comparing the results with atomic absorption spectrometry (AAS) analysis.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Ziyi He, Justin Huffman, Kathrine Curtin, Krista L. Garner, Elizabeth C. Bowdridge, Xiaojun Li, Timothy R. Nurkiewicz, Peng Li
Summary: The cPlate system, enabled by a novel microscale fluid manipulation method, allows for highly scalable multiplexed ELISA analysis with high sensitivity and specificity, shorter assay time, and lower consumption, making it suitable for point-of-care testing in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
E. Unlu, G. Turna Saltoglue, G. Unlu, H. Tozak Yildiz
Summary: This study determined the AFM1 levels in breast milk samples collected from mothers who gave birth in Kirsehir, Turkey. The results showed that the AFM1 levels in breast milk were lower in mothers who did not consume milk, mothers consuming fabrication milk had lower AFM1 levels compared to mothers consuming homemade milk (p<0.01), and mothers consuming homemade or self-made bread had lower AFM1 levels (p<0.05).
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)
Article
Immunology
A-Yeung Jang, Min-Joo Choi, Yong Zhi, Hyun-Jung Ji, Ji-Yun Noh, Jin-Gu Yoon, Hee-Jin Cheong, Woo-Joo Kim, Ho-Seong Seo, Joon-Young Song
Summary: The standardized GBS-ELISA developed in this study shows high specificity and accuracy in evaluating the immunogenicity of GBS vaccines against the three main serotypes. The results demonstrate good performance of the ELISA in assessing the three serotypes, aiding in the development and evaluation of GBS vaccines.
Article
Medicine, General & Internal
Lotfi S. Bin Dahman, Khalid M. Sumaily, Essa M. Sabi, Mohammed A. Hassan, Abeer M. Bin Thalab, Asrar S. Sayad, Saleh M. Bin Kolaib, Fatima M. Alhadhrmi
Summary: This study compared the serum ferritin levels measured by ELISA, Cobas e411, and Cobas Integra 400 methods. The results showed no significant difference between ELISA and Cobas e411, while the values from Cobas Integra 400 were significantly higher. Cobas e411 and Cobas Integra 400 methods demonstrated higher sensitivity, specificity, and accuracy. Further investigations with larger samples are needed to determine interchangeability.
Article
Agriculture, Dairy & Animal Science
Ping Chen, Mengjun Zhou, Xirui Chen, Sicheng Xiong, Yu Su, Hui Zhou, Juan Peng, Yonghua Xiong
Summary: A novel immunochromatographic assay was developed using highly luminescent quantum dot beads as signal amplification probe for the rapid and sensitive determination of Staphylococcal enterotoxin A (SEA) in pasteurized milk. The assay exhibited high sensitivity, good dynamic linearity, and selectivity, making it a powerful tool for the quantitative detection of SEA contamination in real milk samples.
JOURNAL OF DAIRY SCIENCE
(2022)
Review
Nanoscience & Nanotechnology
Yuqian Guo, Liang Guo, Yu Su, Yonghua Xiong
Summary: This review provides an overview of the CRISPR-Cas system as a molecular manipulation technology and nanomaterials as novel signal transducers for early cancer diagnosis. It summarizes the working mechanisms of the CRISPR-Cas system and the various signal models used for cancer diagnosis, and discusses the prospects and challenges of CRISPR-Cas-based nanosensors.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Caicai Fu, Tianying Lu, Xiao Dai, Ping Ding, Yonghua Xiong, Jun Ge, Xiaoyang Li
Summary: In this study, a chemoenzymatic cascade reaction using a hybrid catalyst is proposed for the efficient and green removal of mycotoxins. The GOx-Fe3O4@COF hybrid catalyst, synthesized by compartmental co-immobilized glucose oxidase (GOx) and Fe3O4 nanoparticles (NPs) on a flower-shaped covalent organic framework (COF), exhibits excellent activity in mycotoxin removal. The degradation efficiency of aflatoxin B-1 (AFB(1)) is significantly higher in the chemoenzymatic cascade reaction, catalyzed by GOx-Fe3O4@COF, compared to the Fenton reaction catalyzed by Fe3O4@COF. The GOx-Fe3O4@COF hybrid catalyst shows high activity in a wide pH range of 3.0-7.0, overcoming the pH limitation of the Fenton reaction.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Food Science & Technology
Weipeng Tong, Hanpeng Xiong, Hao Fang, Yuhao Wu, Haichuan Li, Xiaolin Huang, Yuankui Leng, Yonghua Xiong
Summary: In order to achieve high-throughput analysis of mycotoxins, there is a great need for point-of-care (POC) methods that do not require expensive instruments or specialized technicians. The conventional enzyme-linked immunosorbent assay (ELISA), which is widely used for screening, has low sensitivity and is affected by competing antigens. In this study, we developed a plasmonic-photothermal ELISA that uses color-temperature dual-modal signals for highly sensitive detection of ochratoxin A (OTA), with the signal amplified by a bifunctional M13 phage. Under optimal conditions, the colorimetric and photothermal signals showed good dynamic linearity for quantitative OTA detection, with detection limits of 12.1 and 8.6 pg mL(-1), respectively. Additionally, the proposed ELISA could be visually determined with a cutoff limit of 78 pg mL(-1) based on a distinct color change from deep blue to red. The method also showed high accuracy and robustness when applied to OTA-spiked corn samples. Overall, this strategy provides a promising method for eco-friendly and sensitive POC screening of OTA, and can be easily adapted for other analytes by changing the specific mimotope sequence.
Article
Food Science & Technology
Hanpeng Xiong, Ping Chen, Xirui Chen, Xuanang Shen, Xiaolin Huang, Yonghua Xiong, Yu Su
Summary: In this study, a novel aggregation-induced emission (AIE)-based sandwich lateral flow immunoassay (LFIA) was introduced for the sensitive detection of Staphylococcal enterotoxin A (SEA) using red-emissive AIE nanoparticles (AIENPs) as the fluorescent nanoprobe. The ultrasensitive detection of SEA in pasteurised milk was achieved within 20 min with a limit of detection of 0.04 ng mL(-1). The proposed PBA-AIENP-LFIA platform shows promise as a potent tool for the identification of additional compounds in food samples as well as an ideal test method for on-site detections.
Article
Food Science & Technology
Ge Xu, Xiaojing Fan, Xirui Chen, Zilong Liu, Guoxin Chen, Xiaxia Wei, Xiangmin Li, Yuankui Leng, Yonghua Xiong, Xiaolin Huang
Summary: In this study, ultrabright fluorescent microbeads (AIEMBs) were developed by encapsulating a green-emitting fluorophore with aggregation-induced emission (AIE) characteristic in polymer nanoparticles. The AIEMBs were then used as signal reporters in a competitive LFIA for the highly sensitive screening of fumonisin B1 (FB1) in corn samples. The AIE-LFIA demonstrated high sensitivity, excellent selectivity, good accuracy, and high reliability, indicating a promising platform for FB1 screening.
Article
Agriculture, Multidisciplinary
Mengjun Zhou, Xirui Chen, Xuan-ang Shen, Xiangkai Lin, Ping Chen, Zhaohui Qiao, Xiangmin Li, Yonghua Xiong, Xiaolin Huang
Summary: Highly luminescent aggregation-induced emission luminogens embedded nanospheres (AIENPs) were introduced as signal amplification probes of lateral flow immunoassay (LFIA) for quantitative detection of ZEN. Compared with time-resolved dye embedded nanoparticles (TRNPs), AIENPs showed stronger photoluminescence intensity and superior environmental tolerance. AIENP-LFIA exhibited better dynamic linearity and lower detection limit than TRNP-LFIA, making it a practical method for rapid, sensitive, specific, and accurate quantitative detection of ZEN in corn samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Tianying Lu, Caicai Fu, Yonghua Xiong, Zheling Zeng, Yunkai Fan, Xiao Dai, Xiaolin Huang, Jun Ge, Xiaoyang Li
Summary: AFB1 contamination in edible oils is a significant concern for safety. Enzymatic degradation provides a promising method, but its efficiency is hindered by low enzyme activity and stability. In this study, an amphipathic laccase-inorganic hybrid nanoflower (Lac NF-P) was developed as a biocatalyst to degrade AFB1 in peanut oil. Lac NF-P exhibited 134- and 3.2-fold higher degradation efficiency compared to laccase and Lac NF, respectively. Within 3 hours, Lac NF-P reduced AFB1 concentration from 50-150 μg/kg to less than 0.96 μg/kg in peanut oil without affecting its quality. This study opens up new possibilities for developing biocatalysts to detoxify mycotoxins in edible oils.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wenchao Zhan, Yu Su, Xirui Chen, Hanpeng Xiong, Xiaxia Wei, Xiaolin Huang, Yonghua Xiong
Summary: A hydrogel probe encapsulating aggregation-induced emission luminogen (AIEgen) was developed for ultrasensitive detection of Hg2+, with improved sensitivity through the use of hydrophilic hydrogels and spatial networks. The probe exhibited high specificity, accuracy and precision in detecting Hg2+ in real-world water samples, with a sensitivity 4-fold higher than that of pure AIEgen probes. This TR hydrogel-based chemosensor holds promising potential as a robust, fast and effective platform for sensitive Hg2+ detection.
Article
Chemistry, Multidisciplinary
Hao Fang, Yaofeng Zhou, Yanbing Ma, Qi Chen, Weipeng Tong, Shengnan Zhan, Qian Guo, Yonghua Xiong, Ben Zhong Tang, Xiaolin Huang
Summary: This study presents a highly sensitive quantitative detection strategy for low-abundance target analytes by utilizing the synergistic combination of M13 phage-mediated leverage effect and GISG-amplified light scattering signal modulation. The strategy achieves ultrasensitive and rapid quantification of target analytes and displays about 4 orders of magnitude enhancement in sensitivity compared to traditional methods.
Article
Chemistry, Analytical
Qian Guo, Jun Huang, Hao Fang, Xiaoyang Li, Yu Su, Yonghua Xiong, Yuankui Leng, Xiaolin Huang
Summary: In this study, an ultrasensitive dynamic light scattering (DLS) immunosensor was proposed for the detection of Salmonella. The sensor utilized a cargo release-seed growth strategy enabled by gold nanoparticle-decorated covalent organic frameworks (COF@AuNP) to generate a significantly enhanced light-scattering signal, resulting in improved detection sensitivity. The immunosensor could detect trace amounts of Salmonella in milk in the range of 2.0 x 10(2)-2.0 x 10(5) CFU mL(-1), with a limit of detection of 60 CFU mL(-1). It also demonstrated excellent selectivity, accuracy, precision, and reliability for detecting Salmonella in milk.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
