Article
Genetics & Heredity
Lu Lu, Shaotong Dai, Lulu Liu, Jiaxi Liu, Xin Zhang, Xiaoli Huang, Ping Ouyang, Yi Geng, Zhiqiong Li, Defang Chen
Summary: The study identified HMGB1 and HMGB2 in Acipenser baerii and found that these proteins play important roles in DNA damage repair, immune response, and inflammation. The expression of these proteins was detected in multiple tissues and was significantly induced after infection and PAMPs treatment, suggesting their involvement in the pathogenic infection process. This provides a basis for further understanding the mechanism of enteritis induced by Streptococcus iniae in Acipenser baerii.
Article
Fisheries
Defang Chen, Hao Zhu, Daiyu Chen, Lei Yang, Fei Yang, Qiaolin Pei, Yue He, Xin Zhang, Lianshi Qu, Tianqiang Liu, Yi Geng, Zhiqiong Li
Summary: The study found that Salvia miltiorrhiza polysaccharides (SMP) have positive effects on the growth performance, antioxidant capacity, non-specific immunity, and resistance to Streptococcus iniae in hybrid sturgeon. The dietary supplementation of SMP can enhance the antioxidant and non-specific immune capacity of hybrid sturgeon, improve disease resistance, and promote healthy breeding. These findings provide novel recommendations for the application of SMP supplementation in farmed fish.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Lu Lu, Shaotong Dai, Hao Zhu, Xin Zhang, Zhiqiong Li, Xiaoli Huang, Ping Ouyang, Yi Geng, Defang Chen
Summary: Receptor-interacting serine/threonine-protein kinase 2 (RIPK2) plays an important role in regulating inflammatory response and resisting pathogenic microbial infection. The study identified AbRIPK2 in Acipenser baerii and found that it is highly expressed in the gill and immune tissues. AbRIPK2 was significantly upregulated after infection and cellular stimulation, indicating its potential mechanism in resisting pathogenic infection in Siberian sturgeon.
JOURNAL OF FISH BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Esther Vazquez-Fernandez, Blanca Chinchilla, Agustin Rebollada-Merino, Lucas Dominguez, Antonio Rodriguez-Bertos
Summary: This article discusses a mortality outbreak in sturgeons caused by Aeromonas salmonicida in Spain. The study found that sturgeons are susceptible to this pathogen and exhibit a hyperacute form of the disease without specific clinical signs. The findings contribute to a better understanding of aeromoniasis in sturgeons and its impact on the aquaculture and caviar industries.
Article
Fisheries
Serik Bakiyev, Izat Smekenov, Irina Zharkova, Saidina Kobegenova, Nurlan Sergaliyev, Gaisa Absatirov, Amangeldy Bissenbaev
Summary: This study isolated and identified dominant bacteria in diseased Siberian sturgeon and determined their pathogenicity on other fish species. The analysis of virulence genes and drug sensitivity provides important information for future research on pathogens in Siberian sturgeon aquaculture.
AQUACULTURE REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Hongsen Xu, Xiaoni Wang, Qianrong Liang, Ruiping Xu, Jun Liu, Denghang Yu
Summary: This study explored the effects of dietary chitosan on the growth, antioxidant activity, immunity, intestinal morphology, and resistance to Aeromonas hydrophila in hybrid sturgeon. The results indicated that chitosan supplementation significantly improved the growth performance, disease resistance, and intestinal health of the sturgeon. The optimum dosage of chitosan was 3.00 g/kg diet.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Naeemeh Salimi Khorshidi, Amir Parviz Salati, Saeed Keyvanshokooh
Summary: The study showed that BPA affects the expression of proteins in the liver of large fish, leading to the activation of detoxification and metabolism pathways, as well as liver necrosis. In addition, changes in plasma and mucus vitellogenin levels were positively correlated with BPA dosage.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Fisheries
Jiaxi Liu, Lu Lu, Lulu Liu, Daiyu Chen, Fei Yang, Yi Geng, Zhiqiong Li, Xiaoli Huang, Ping Ouyang, Jun Wang, Defang Chen
Summary: This study cloned and characterized the NLRC3-like gene from Siberian sturgeon, showing its potential critical role in the immune response of sturgeon during pathogen challenge. The expression pattern of AbNLRC3-like varies in different tissues and stages of viral infection, indicating its importance in immune regulation.
FISH AND SHELLFISH IMMUNOLOGY REPORTS
(2021)
Article
Fisheries
Eman M. Moustafa, Foad A. Farrag, Mahmoud A. O. Dawood, Khalid Shahin, Ahmad Hamza, Olivier Decamp, Radi Mohamed, Mabrouk Elsabagh, Mahmoud Eltholth, Amira A. Omar
Summary: The study demonstrated the immunomodulatory effect of probiotic feed against Streptococcus iniae infection in Nile tilapia, showing potential for disease control on farms by enhancing haematological parameters and reducing mortality rates in the infected fish.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Joseph F. A. Varga, Sascha R. Brunner, Grant Cheng, Daniel Min, Marc G. Aucoin, Andrew C. Doxey, Brian Dixon
Summary: In this study, a novel antimicrobial peptide (AMP) derived from rainbow trout von Willebrand Factor was identified using computational methods, showing antibacterial activity against Streptococcus iniae. The findings improve understanding of fish immune responses towards zoonotic pathogens.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Hamed Ghafarifarsani, Ghasem Rashidian, Atefeh Sheikhlar, Mehdi Naderi Farsani, Seyed Hossein Hoseinifar, Hien Van Doan
Summary: The experimental results demonstrate that dietary oak acorn extract has a significant impact on immune responses and antibacterial activity of skin mucus in rainbow trout, particularly in enhancing lysozyme and agglutination activities.
AQUACULTURE RESEARCH
(2021)
Article
Fisheries
Blanca Chinchilla, Esther Vazquez-Fernandez, Agustin Rebollada-Merino, Marta Perez-Sancho, Lucas Dominguez, Antonio Rodriguez-Bertos
Summary: In this study, Flavobacterium psychrophilum was identified as the causative agent of mortality in diseased juvenile Siberian sturgeons using MALDI-TOF MS and nested PCR. The infected sturgeons showed lethargy, dark skin pigmentation, increased mucus production, and skin ulcerations and haemorrhages. Histological examination revealed various pathological findings, including branchitis, dermatitis, myositis, lymphoid tissue atrophy, and organ degeneration. This is the first report of F. psychrophilum infection in Siberian sturgeons, and the findings contribute to understanding the bacterium's pathogenicity and susceptible fish species range.
JOURNAL OF FISH DISEASES
(2023)
Article
Microbiology
Lizi Yin, Chao Liang, Wenyan Wei, Shuanghui Huang, Yongqiang Ren, Yi Geng, Xiaoli Huang, Defang Chen, Hongrui Guo, Jing Fang, Huidan Deng, Weimin Lai, Shumin Yu, Ping Ouyang
Summary: This study evaluated the antibacterial activity and potential mechanism of thymol against Streptococcus iniae. Thymol exhibited potent antibacterial activity, damaging the cell membrane and wall and disrupting DNA replication and transcription in S. iniae. In vivo studies showed that thymol had a protective effect on S. iniae infection, reducing mortality and colonization of the bacteria in tissues, and increasing immune enzyme activities. These findings suggest that thymol may be a candidate agent to replace traditional antibiotics for the prevention and treatment of S. iniae infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Nathaniel W. Farris, Ali Hamidoghli, Jinho Bae, Seonghun Won, Wonsuk Choi, Janka Biro, Seunghyung Lee, Sungchul C. Bai
Summary: This study found that supplementing the diet of juvenile olive flounder with an appropriate amount of GABA significantly improved their growth, immune function, and ability to resist specific bacterial infections. These results demonstrate that GABA is an important functional feed additive.
Article
Fisheries
Seyed Pezhman Hosseini Shekarabi, Mehdi Shamsaie Mehrgan, Farshad Ramezani, Mahmoud A. O. Dawood, Hien Van Doan, Tossapol Moonmanee, Noor Khalidah Abdul Hamid, Zulhisyam Abdul Kari
Summary: The study found that the dietary effect of Barberry fruit (BF) extract can improve the immune and antioxidant defense systems in Siberian sturgeon, enhancing their antibacterial capacity and reducing stress responses.
AQUACULTURE REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Jieyao Zhu, Hong Wang, Jun Wang, Xingli Wang, Shuang Peng, Yi Geng, Kaiyu Wang, Pin Ouyang, Zhiqiong Li, Xiaoli Huang, Defang Chen
MOLECULAR IMMUNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Yang Feng, Xiaoli Huang, Yajiao Duan, Wei Fan, Jing Duan, Kaiyu Wang, Yi Geng, Ping Ouyang, Yongqiang Deng, Defang Chen, Shiyong Yang
BIOMED RESEARCH INTERNATIONAL
(2018)
Article
Cell Biology
Xiaoli Huang, Yang Feng, Hong Tang, Guanqing Xiong, Liangyu Li, Yucen Yang, Kaiyu Wang, Ping Ouyang, Yi Geng, Defang Chen, Shiyong Yang
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2019)
Article
Fisheries
Jiaxi Liu, Fei Yang, Shuang Peng, Yi Geng, Xiaoli Huang, Ping Ouyang, Zhiqiong Li, Defang Chen
AQUACULTURE RESEARCH
(2020)
Article
Fisheries
Daiyu Chen, Lei Yang, Fei Yang, Qiaolin Pei, Lu Lu, Xiaoli Huang, Ping Ouyang, Yi Geng, Zhiqiong Li, Xin Zhang, Jun Wang, Defang Chen
Summary: This study found that Salvia miltiorrhiza polysaccharide (SMP) can improve the immune function and disease resistance of sturgeon. In vitro experiments showed that SMP could activate the macrophages of sturgeon, while in vivo experiments demonstrated that dietary supplementation with SMP enhanced immune cell activity and resistance to bacterial infection in sturgeon.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Behavioral Sciences
Defang Chen, Yingzi Li, Hongwei Wu, Yuanbing Wu, Ni Tang, Shuhuang Chen, Youlian Liu, Jun Wang, Xin Zhang, Zhiqiong Li
Summary: This study investigates the feeding regulation pathway of Ghrelin and CB1 in Siberian sturgeon. The results show that Ghrelin promotes food intake in sturgeons by inducing the expression of NPY in the diencephalon, and this effect is reversed by cannabinoid receptor antagonism.
HORMONES AND BEHAVIOR
(2022)
Article
Fisheries
Defang Chen, Lu Lu, Hong Wang, Shuang Peng, Jiaxi Liu, Xin Zhang, Zhiqiong Li, Xiaoli Huang, Ping Ouyang, Lianshi Qu, Yi Geng
Summary: This study investigated the structure and function of HMGB1 in the cyprinid fish Schizothorax prenanti. The results showed that SpHMGB1 was universally expressed in different tissues and played an important role in the immune response by activating respiratory burst and up-regulating inflammatory genes expression.
JOURNAL OF AQUATIC ANIMAL HEALTH
(2022)
Article
Fisheries
Lu Lu, Shaotong Dai, Hao Zhu, Xin Zhang, Zhiqiong Li, Xiaoli Huang, Ping Ouyang, Yi Geng, Defang Chen
Summary: Receptor-interacting serine/threonine-protein kinase 2 (RIPK2) plays an important role in regulating inflammatory response and resisting pathogenic microbial infection. The study identified AbRIPK2 in Acipenser baerii and found that it is highly expressed in the gill and immune tissues. AbRIPK2 was significantly upregulated after infection and cellular stimulation, indicating its potential mechanism in resisting pathogenic infection in Siberian sturgeon.
JOURNAL OF FISH BIOLOGY
(2023)
Article
Fisheries
Defang Chen, Lu Lu, Qiaolin Pei, Daiyu Chen, Lei Yang, Xin Zhang, Xiaoli Zhang, Ping Ouyang, Yi Geng, Zhiqiong Li, Liangyu Li, Xiaoli Huang
Summary: This study analyzed the immunological effects of Salvia miltiorrhiza polysaccharides (SMP) on the hemocytes of Procambarus clarkii. The results showed that SMP treatment upregulated the activity of total superoxide dismutase (T-SOD), phenoloxidase (PO), and respiratory burst. Differential gene expression analysis revealed that SMP affected crustacean cellular immune-related signaling pathways. Furthermore, diets supplemented with SMP improved the cellular and humoral immunity of crayfish. This study provides molecular evidence for the immunopotentiator effects of plant polysaccharides in crustaceans.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Lu Lu, Shaotong Dai, Lulu Liu, Jiaxi Liu, Xin Zhang, Xiaoli Huang, Ping Ouyang, Yi Geng, Zhiqiong Li, Defang Chen
Summary: The study identified HMGB1 and HMGB2 in Acipenser baerii and found that these proteins play important roles in DNA damage repair, immune response, and inflammation. The expression of these proteins was detected in multiple tissues and was significantly induced after infection and PAMPs treatment, suggesting their involvement in the pathogenic infection process. This provides a basis for further understanding the mechanism of enteritis induced by Streptococcus iniae in Acipenser baerii.
Article
Fisheries
Defang Chen, Hao Zhu, Daiyu Chen, Lei Yang, Fei Yang, Qiaolin Pei, Yue He, Xin Zhang, Lianshi Qu, Tianqiang Liu, Yi Geng, Zhiqiong Li
Summary: The study found that Salvia miltiorrhiza polysaccharides (SMP) have positive effects on the growth performance, antioxidant capacity, non-specific immunity, and resistance to Streptococcus iniae in hybrid sturgeon. The dietary supplementation of SMP can enhance the antioxidant and non-specific immune capacity of hybrid sturgeon, improve disease resistance, and promote healthy breeding. These findings provide novel recommendations for the application of SMP supplementation in farmed fish.
AQUACULTURE INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Defang Chen, Yinqiu Chen, Lu Lu, Hao Zhu, Xin Zhang, Xiaoli Huang, Zhiqiong Li, Ping Ouyang, Xiaoli Zhang, Liangyu Li, Yi Geng
Summary: In this study, the inflammatory response mechanism and characterization of NOD-like receptors (NLRs) family in Siberian sturgeon were investigated using transcriptome analysis. The results showed differential expression of genes related to pattern recognition receptors, adaptor proteins, cytokines, and cell adhesion molecules. The downregulation of NOD-like receptor family CARD domains containing 3-like (NLRC3-like) and upregulation of pro-inflammatory cytokines were observed. Furthermore, 19 NLRs with NACHT structural domains were identified in Siberian sturgeon. This study provides insights into the inflammatory response and NLRs family in teleost fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Defang Chen, Fei Yang, Lei Yang, Qiaolin Pei, Xin Zhang, Xiaoli Huang, Ping Ouyang, Zhiqiong Li, Yi Geng
Summary: The rapid development of sturgeon aquaculture has met the market demand for sturgeon products and addressed the issue of declining wild resources. However, infectious deaths after artificial propagation of female Siberian sturgeon pose a threat to sturgeon farming. This study identified Aeromonas hydrophila as the etiological agent causing the infection, while co-isolated Lactococcus petauri showed resistance to A. hydrophila infection. The findings contribute to a better understanding of parental sturgeon infection and provide insights for the prevention of Aeromonas infection through the use of antagonistic strains.
Article
Fisheries
Shuang Peng, JiaXi Liu, YunDan Pu, Hong Wang, Yi Geng, KaiYu Wang, ZhiQiong Li, ZongJun Du, DeFang Chen
ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
(2018)