期刊
FISH & SHELLFISH IMMUNOLOGY
卷 105, 期 -, 页码 135-143出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2020.06.023
关键词
Immune response; Epinephelus coioides; Pseudomonas plecoglossicida; impB; RNA-seq; RNAi
资金
- National Natural Science Foundation of China [31672694, 31972836]
- Science and Technology Program of Xiamen Southern Oceanographic Center [19CZP003HJ07]
- Fujian provincial special fund for marine and fishery protection and development [MCZ[2019]062]
- open fund of Fujian Province Key Laboratory of Special Aquatic Formula Feed [2019-01]
Pseudomonas plecoglossicida is a Gram-negative bacterium that causes visceral white spot disease in Epinephelus coioides and leads to severe aquatic economic losses. The RNA-seq results of a previous study showed that the expression of the impB gene in P. plecoglossicida was significantly upregulated during infection. Four shRNAs were designed and synthesized to silence the impB gene in P. plecoglossicida, and the maximum silencing efficiency was 95.2%. Intraperitoneal injection of the impB-RNAi strain of P. plecoglossicida did not cause E. coioides death, and the spleens of infected fish did not show significant clinical symptoms. Although the injection of the mutant strain increased the antibody titer in E. coioides serum, it could not effectively protect E. coioides against wild strain infection. Compared with E. coioides infected with the wild type strain, the RNA-seq results for E. coioides infected with the impB-RNAi strain differed greatly. The KEGG enrichment analysis showed that key genes of the chemokine signalling pathway of E. coioides were downregulated by the silencing of impB in P. plecoglossicida. Infection with the impB-RNAi strain of P. plecoglossicida through injection did not produce good immune protection against E. coioides. The present study provides a novel insight into the immune responses of E. coioides to the impB gene of P. plecoglossicida.
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