Article
Endocrinology & Metabolism
Malak El Sabeh, Subbroto Kumar Saha, Sadia Afrin, Mostafa A. Borahay
Summary: The study revealed that simvastatin significantly reduced the expression of Wnt4 and LRP5 in leiomyoma cells, as well as decreasing the levels of total beta-catenin and nonphosphorylated beta-catenin. Simvastatin also inhibited the effects of estrogen and progesterone on the Wnt/beta-catenin pathway, as well as the expression of c-Myc. These results suggest that simvastatin may have a beneficial effect on uterine leiomyoma by suppressing the overactive Wnt/beta-catenin pathway.
Article
Endocrinology & Metabolism
Maria Cristina Carbajo-Garcia, Ana Corachan, Marina Segura-Benitez, Javier Monleon, Julia Escrig, Amparo Faus, Antonio Pellicer, Irene Cervello, Hortensia Ferrero
Summary: The research showed that treatment with 5-aza-CdR can reduce cell proliferation and extracellular matrix formation in uterine leiomyoma cells, as well as inhibit the Wnt/beta-catenin signaling pathway. Therefore, DNA methylation inhibition may be a potential therapeutic target for uterine leiomyoma.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Maria Cristina Carbajo-Garcia, Elena Juarez-Barber, Marina Segura-Benitez, Amparo Faus, Alexandra Trelis, Javier Monleon, Greta Carmona-Antonanzas, Antonio Pellicer, James M. Flanagan, Hortensia Ferrero
Summary: This study found that instability in H3K4me3 histone modification alters the expression of oncogenes and tumor suppressor genes, leading to malignant proliferation of uterine leiomyomas and dysregulated Wnt/beta-catenin and TGF-beta pathways. Reversal of these histone modifications may provide a promising new therapeutic approach for uterine leiomyoma patients.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Cell Biology
Malak El Sabeh, Subbroto Kumar Saha, Sadia Afrin, Md Soriful Islam, Mostafa A. Borahay
Summary: Uterine leiomyoma is the most common tumor of the female reproductive system, with poorly understood underlying mechanisms, but alterations in signaling pathways are key players in its pathobiology. The Wnt/beta-catenin pathway plays a crucial role in leiomyoma development, with specific molecules within this pathway showing potential as therapeutic targets.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Tsai-Der Chuang, Derek Quintanilla, Drake Boos, Omid Khorram
Summary: The lncRNA MIAT plays a crucial role in leiomyoma pathogenesis by regulating the expression of the miR-29 family, leading to downstream effects on collagen and TGF-β3. These findings highlight the physiological significance of MIAT in extracellular matrix accumulation in leiomyoma.
Article
Endocrinology & Metabolism
Tsai-Der Chuang, Derek Quintanilla, Drake Boos, Omid Khorram
Summary: This study found that MIAT plays a regulatory role on the miR-29 family in leiomyomas, affecting the expression levels of collagen and TGF-β3.
Article
Biochemistry & Molecular Biology
Mingliang He, Xiaoyang Lv, Xiukai Cao, Zehu Yuan, Tesfaye Getachew, Yutao Li, Shanhe Wang, Wei Sun
Summary: SRY-box transcription factor 18 (SOX18) is expressed in dermal papilla cells (DPCs) of sheep hair follicles and plays a crucial role in promoting DPC proliferation through activating the Wnt/β-Catenin signaling pathway. Inhibition of the Wnt/β-Catenin signaling pathway can be rescued by SOX18, suggesting the essential role of SOX18 in governing DPC proliferation and hair growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tsai-Der Chuang, Jianjun Gao, Derek Quintanilla, Hayden McSwiggin, Drake Boos, Wei Yan, Omid Khorram
Summary: Recent studies have found that somatic mutations in exon 2 of MED12 gene occur at a frequency of up to 80% and play a functional role in leiomyoma development. This study aimed to investigate the expression profile of RNA transcripts in leiomyomas with and without these mutations, as well as in their paired myometrium. Using next-generation RNA sequencing, the researchers found that there were 394 genes differentially and aberrantly expressed only in the mutated tumors. These genes mostly regulated the components of the extracellular matrix. Additionally, although the myometrium did not have MED12 mutations, there were significant differences in the transcriptome landscape compared to non-mutated specimens, with genes related to the response to oxygen-containing compounds being most affected.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Mingliang He, Xiaoyang Lv, Xiukai Cao, Zehu Yuan, Kai Quan, Tesfaye Getachew, Joram M. M. Mwacharo, Aynalem Haile, Yutao Li, Shanhe Wang, Wei Sun
Summary: In our study, we found that CRABP2 was highly expressed in Hu sheep hair follicles, indicating its potential influence on dermal papilla cells (DPCs). Our experiments demonstrated that overexpression of CRABP2 promoted DPC proliferation and activated the Wnt/beta-catenin pathway. These findings highlight the crucial role of CRABP2 in Hu sheep DPC proliferation and shed light on its function in hair follicles.
Article
Plant Sciences
Linwei Chen, Honglin Chen, Qiaowei Yang, Yong Jiang, Lunyuan Liu, Hui Yu, Yan Chen, Jindong Li, Nan Chen, Hua Wang, Qin Wang
Summary: This study revealed that Guizhi Fuling Capsule (GFC) inhibited the growth of uterine fibroids (UFs) by modulating the Med12-mediated Wnt/ll-Catenin signaling pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Azna Zuberi, Yongchao Huang, Ariel J. Dotts, Helen Wei, V. John S. Coon, Shimeng Liu, Takashi Iizuka, Olivia Wu, Olivia Sotos, Priyanka Saini, Debabrata Chakravarti, Thomas G. Boyer, Yang Dai, Serdar E. Bulun, Ping Yin
Summary: This study reveals the association between mutMED12 and the activation of the TDO2/kynurenine/AHR pathway in the growth of uterine leiomyomas. These findings may contribute to the development of targeted treatments and precision medicine for uterine leiomyomas.
Article
Obstetrics & Gynecology
Tsai-Der Chuang, Derek Quintanilla, Drake Boos, Omid Khorram
Summary: This study investigated the expression profile of SE-lncRNAs and associated mRNAs in leiomyomas and paired myometrium, revealing that differential expression of SE contributes to dysregulation of protein coding genes in leiomyomas. The study also found that race and MED12 mutation can influence the expression of certain SE-lncRNAs.
REPRODUCTIVE SCIENCES
(2022)
Article
Cell Biology
Xiaoxuan Duan, Yihuan Chen, Kai Zhang, Wei Chen, Jun Zhao, Xiaoshuo Dai, Wenbo Cao, Ziming Dong, Saijun Mo, Jing Lu
Summary: This study found that phosphoglycerate dehydrogenase (PHGDH) was highly expressed in esophageal squamous carcinoma (ESCC), and high expression was associated with poor prognosis. In vitro and in vivo experiments demonstrated that PHGDH knockdown inhibited ESCC progression by suppressing Wnt/beta-catenin signaling pathway. These results indicate that PHGDH may be a potential therapeutic target for ESCC.
CELLULAR SIGNALLING
(2023)
Article
Agriculture, Multidisciplinary
Duoduo Zhang, Xingtao Zhou, Linyuan Liu, Mi Guo, Tongwen Huang, Wengan Zhou, Fang Geng, Steve W. Cui, Shaoping Nie
Summary: The study revealed that glucomannan from Aloe vera gel promoted proliferation and differentiation of intestinal stem cells through the Wnt/β-catenin signaling pathway, facilitating epithelial cell regeneration and alleviating colitis in mice.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Zhi Fang, Min Zhong, Ling Zhou, Yi Le, Heng Wang, Ziling Fang
Summary: LRP8 is markedly overexpressed in NSCLC tissues and cell lines, correlating with poor clinicopathological characteristics and prognosis. Depletion of LRP8 suppresses proliferation, migration, invasion, and epithelial-mesenchymal transition of NSCLC cells, partly by inhibiting the Wnt/beta-catenin pathway.
Article
Obstetrics & Gynecology
Maria Cristina Carbajo-Garcia, Zaira Garcia-Alcazar, Ana Corachan, Javier Monleon, Alexandra Trelis, Amparo Faus, Antonio Pellicer, Hortensia Ferrero
Summary: The study demonstrates that SAHA inhibits cell proliferation, cell cycle, ECM formation, and TGF-/53 signaling in human uterine leiomyoma primary cells, suggesting the potential utility of histone deacetylase inhibition in the treatment of uterine leiomyoma.
FERTILITY AND STERILITY
(2022)
Article
Endocrinology & Metabolism
Marina Segura-Benitez, Maria Cristina Carbajo-Garcia, Ana Corachan, Amparo Faus, Antonio Pellicer, Hortensia Ferrero
Summary: Successful implantation relies on communication between maternal endometrium and embryo, with EVs playing a role in this process. Ultracentrifugation was found to be the most efficient method for EV isolation from endometrial epithelial cells, with EVs containing proteins related to embryo implantation processes.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Obstetrics & Gynecology
Elena Juarez-Barber, Mauro Cozzolino, Ana Corachan, Diana Alecsandru, Nuria Pellicer, Antonio Pellicer, Hortensia Ferrero
Summary: This study investigated the endometrial receptivity of patients with adenomyosis and the impact of personalized embryo transfer (PET) on IVF outcomes. The results showed that patients with adenomyosis had altered endometrial receptivity, but PET did not significantly improve reproductive outcomes.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Obstetrics & Gynecology
Elena Juarez-Barber, Marina Segura-Benitez, Maria Cristina Carbajo-Garcia, Alba Bas-Rivas, Amparo Faus, Carmen Vidal, Juan Giles, Elena Labarta, Antonio Pellicer, Irene Cervello, Hortensia Ferrero
Summary: This study analyzed the miRNAs present in extracellular vesicles secreted by the endometrium of women with adenomyosis. The results showed that these vesicles contain miRNAs related to adenomyosis, implantation failure, and pregnancy complications. The miRNAs regulate downstream genes involved in adenomyosis progression and impaired embryo implantation.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Review
Biochemistry & Molecular Biology
Maria Gomez-Alvarez, Marcos Agustina-Hernandez, Emilio Frances-Herrero, Adolfo Rodriguez-Eguren, Clara Bueno-Fernandez, Irene Cervello
Summary: Organoids are three-dimensional cellular structures that recreate the biological characteristics of native tissues and organs in vitro, offering distinct advantages over traditional two-dimensional approaches. This literature review clarifies the concept of organoids and addresses the fundamental questions pertaining to their composition, growth, maintenance, and suitability as in vitro models. It also emphasizes the relevance of organoid models in the female reproductive tract for both basic biology and clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Maria Gomez-alvarez, Clara Bueno-Fernandez, Adolfo Rodriguez-Eguren, Emilio Frances-Herrero, Marcos Agustina-Hernandez, Amparo Faus, Fernando Gisbert Roca, Cristina Martinez-Ramos, Amparo Galan, Antonio Pellicer, Hortensia Ferrero, Irene Cervello
Summary: The endometrium plays a vital role in fertility and understanding its physiology is crucial for improving reproductive healthcare. This study presents a hybrid endometrial-derived hydrogel that shows promising potential as a biomaterial for regenerative treatments in reproductive medicine. The hydrogel provides excellent support for hEO culture, enhances hEO differentiation efficiency, and demonstrates in vivo compatibility and stability.
ADVANCED HEALTHCARE MATERIALS
(2023)
Meeting Abstract
Obstetrics & Gynecology
Marina Segura-Benitez, Alba Bas-Rivas, Elena Juarez-Barber, Maria Cristina Carbajo-Garcia, Amparo Faus, Antonio Pellicer, Hortensia Ferrero
FERTILITY AND STERILITY
(2022)
Meeting Abstract
Obstetrics & Gynecology
Ana Corachan, Maria Victoria Victoria Bariani, Qiwei Yang, Tao Bai, Hortensia Ferrero, Ayman Al-Hendy
FERTILITY AND STERILITY
(2022)
Meeting Abstract
Obstetrics & Gynecology
Mauro Cozzolino, Elena Juarez-Barber, Ana Corachan, Diana Alecsandru, Antonio Pellicer, Hortensia Ferrero
FERTILITY AND STERILITY
(2022)
Meeting Abstract
Obstetrics & Gynecology
Elena Juarez-Barber, Marina Segura-Benitez, Maria Cristina Carbajo-Garcia, Alba Bas-Rivas, Amparo Faus, Antonio Pellicer, Hortensia Ferrero
FERTILITY AND STERILITY
(2022)
Article
Obstetrics & Gynecology
Adolfo Rodriguez-Eguren, Lucia De Miguel-Gomez, Emilio Frances-Herrero, Maria Gomez-Alvarez, Amparo Faus, Macarena Gomez-Cerda, Ines Moret-Tatay, Ana Diaz, Antonio Pellicer, Irene Cervello
Summary: Local application of hUC-PRP promotes tissue regeneration and fertility restoration in a murine model of Asherman syndrome and endometrial atrophy (AS/EA). The use of hUC-PRP has high impact and is immunotolerated, making it a promising candidate for treating endometrial pathologies.
HUMAN REPRODUCTION OPEN
(2022)
Meeting Abstract
Obstetrics & Gynecology
Elena Juarez-Barber, Ana Corachan, Emilio Frances-Herrero, Carmina Vidal, Juan Giles, Pilar Alama, Amparo Faus, Antonio Pellicer, Irene Cervello, Hortensia Ferrero
REPRODUCTIVE SCIENCES
(2022)
Meeting Abstract
Obstetrics & Gynecology
Maria Cristina Carbajo-Garcia, Alexandra Trelis, Javier Monleon, Antonio Pellicer, James M. Flanagan, Hortensia Ferrero
REPRODUCTIVE SCIENCES
(2022)