期刊
DNA AND CELL BIOLOGY
卷 39, 期 11, 页码 2005-2016出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/dna.2020.5783
关键词
umbilical cord blood; exosomes; mRNA; lncRNA; gestational diabetes mellitus
资金
- National Natural Science Foundation of China [81600685, 81901517]
- Natural Science Foundation of Jiangsu Province [BK20160141]
- Nanjing Medical Science and Technique Development Foundation [YKK16201, QRX17163]
- 333 high level talents training project of Jiangsu Province
- Six talent peaks project in Jiangsu Province [WSN-121]
- Wuxi Key Medical Disciplines [ZDXK012]
- Medical Innovation Team of Jiangsu Province [CXTDB2017016]
- Wuxi Medical Development Discipline [FZXK001]
- Wuxi Young Medical Talents [QNRC094]
- Young project of Wuxi health and Family Planning Commission [Q201815]
- Jiangsu maternal and child health research project [F2018525]
- Wuxi maternal and child health research project [FYKY201807]
Background and Aims:Exosomes contain numerous RNAs and transfer them between cells or organs, thereby establishing intercellular or interorgan communication. The roles of mRNAs and long noncoding RNAs (lncRNAs) from umbilical cord blood exosomes in gestational diabetes mellitus (GDM) occurrence and fetus growth remain poorly understood. We aimed to establish the differential mRNA and lncRNA expression profiles in umbilical cord blood exosomes from GDM patients compared with normal controls. Results:Using microarray technology, we identified 84 mRNAs and 256 lncRNAs as differentially expressed in umbilical cord blood exosomes of GDM patients compared with controls. The protein-protein interaction network revealed that the differentially expressed mRNAs were associated with glucagon signaling pathway, an important GDM-related pathway. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses were performed for mRNAs associated with differentially expressed lncRNAs. The results indicated that metabolic process, growth, and development were significantly enriched, which are important in GDM development and fetus growth. Moreover, pathway network was constructed to reveal the key pathways in GDM, such as metabolic pathways and insulin signaling pathway. Further lncRNA/miRNA interaction analysis showed that most of the exosomal lncRNAs harbored miRNA binding sites, and some were associated with GDM. Conclusion:These results showed that exosomal mRNAs and lncRNAs are aberrantly expressed in the umbilical cord blood of GDM patients and play potential roles in GDM development and fetus growth.
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