Article
Biology
Leire Egia-Mendikute, Alexandre Bosch, Endika Prieto-Fernandez, So Young Lee, Borja Jimenez-Lasheras, Ana Garcia del Rio, Asier Antonana-Vildosola, Chiara Bruzzone, Maider Bizkarguenaga, Nieves Embade, Ruben Gil-Redondo, Maria Luz Martinez-Chantar, Marcos Lopez-Hoyos, Nicola G. A. Abrescia, Jose M. Mato, Oscar Millet, Asis Palazon
Summary: Egia-Mendikute et al. have developed a multiplexed flow cytometric bead array to detect antibodies reactive to three immunogenic SARS-CoV-2 proteins simultaneously. This assay is more sensitive than ELISA and has superior potential to detect low antibody titers, even in COVID-19-negative individuals.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Analytical
Jie Liu, Jinpeng Mao, Mengyu Hou, Zhian Hu, Gongwei Sun, Sichun Zhang
Summary: This article presents a rapid and highly specific method for profiling COVID-19 associated antigen. The method utilizes a nanoparticle scaffold and binding-induced DNA assembly mechanism, with fluorescence translation for detection. The study demonstrates that this method can quantify nucleocapsid protein within 3 minutes, with high recognition ratio and low limit of detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Medicine, General & Internal
Jidapa Szekely, Jenureeyah Mongkolprasert, Nitikorn Jeayodae, Chadarat Senorit, Panuttha Chaimuti, Piyawut Swangphon, Natthaphon Nanakorn, Teerapat Nualnoi, Paweena Wongwitwichot, Theerakamol Pengsakul
Summary: The study developed and evaluated an antigen rapid diagnostic test (the Kestrel(TM) COVID-19 Ag Rapid Test) for professional use. The test showed high sensitivity, low limit of detection, and high accuracy in detecting and managing COVID-19, making it a potential tool for screening, surveillance, and infection control in global SARS-CoV-2 variant outbreaks.
Article
Medicine, General & Internal
Dorian Petonnet, Stephane Marot, Isabelle Leroy, Julien Cohier, Charline Ramahefasolo, Safietou Mansaly, Vincent Calvez, Anne-Genevieve Marcelin, Sonia Burrel
Summary: This study compared the performance of the DiaSorin (TM) LiaisonXL automated quantitative antigen test and the AAZ (TM) rapid antigen test with the DiaSorin (TM) MDX RT-PCR assay. The study found that both antigen tests had similar sensitivity and comparable performance for the detection of SARS-CoV-2 variants.
Article
Biochemical Research Methods
Xuechen Dou, Zhiwei Zhang, Bo Liu, Chao Li, Yaohua Du, Feng Tian
Summary: Nucleocapsid protein (NP) of SARS-CoV-2 is a stable, well-conserved, highly immunogenic, and abundantly expressed structural protein, making it a promising biomarker for early and rapid identification and diagnosis. A novel single-molecule fluorescence-linked immunosorbent assay (FLISA) was developed for highly sensitive detection of NP using streptavidin-modified microplates. FLISA demonstrated a linear detection range of 0.2 pg/mL to 200 ng/mL and a limit of detection (LOD) of 0.73 fg/mL and 8 fg/mL for NP in phosphate buffer saline and spiked nasal swab samples, respectively. The sensitivity of FLISA was superior to commercial antigen detection kits, suggesting its potential application in clinical diagnosis and environmental monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Rebekka F. Thudium, Malene P. Stoico, Estrid Hogdall, Julie Hogh, Henrik B. Krarup, Margit A. H. Larsen, Poul H. Madsen, Susanne D. Nielsen, Sisse R. Ostrowski, Amanda Palombini, Daniel B. Rasmussen, Niels T. Foged
Summary: The newly developed ELISA showed high diagnostic sensitivity and specificity in detecting SARS-CoV-2 nucleocapsid protein, making it a suitable laboratory diagnostic test for COVID-19, particularly in hospitals to facilitate screening of patients, prevention of nosocomial infections, and reduce the requirement for laborious swab sampling and confirmatory PCR analysis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Immunology
Ariel Isaacs, Alberto A. Amarilla, Julio Aguado, Naphak Modhiran, Eduardo A. Albornoz, Alireza A. Baradar, Christopher L. D. McMillan, Jovin J. Y. Choo, Adi Idris, Aroon Supramaniam, Nigel A. J. McMillan, David A. Muller, Paul R. Young, Trent M. Woodruff, Ernst J. Wolvetang, Keith J. Chappell, Daniel Watterson
Summary: Due to the high mutability of SARS-CoV-2, current spike-specific immune detection methods have limitations. This study demonstrates the use of nucleocapsid-specific detection in multiple assays to detect different SARS-CoV-2 variants, providing a universal target for addressing COVID-19 mutations.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Cong Han, Wenping Xing, Wenjin Li, Xiaona Fang, Jian Zhao, Feng Ge, Wei Ding, Pengpeng Qu, Zhaofeng Luo, Liyun Zhang
Summary: In this study, we proposed a biomimetic clamp assay for detecting SARS-CoV2 nucleocapsid protein (Np). The assay utilizes a pair of Np aptamers connected via a T20 spacer to form a DNA biomimetic clamp (DNA-BC). The DNA-BC is anchored on the surface of a screen-printed gold electrode, resulting in a clamp-type impedimetric aptasensor. The aptasensor shows high sensitivity and specificity for Np detection, making it a potentially promising strategy for developing reliable antigen rapid diagnostic testing (Ag-RDT) products to address the current epidemic.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Ching-Chou Wu, Yu-Huan Chiang, Hsin-Yu Chiang
Summary: This study developed an EIS-based immunosensor for the detection of SARS-CoV-2 nucleocapsid protein in saliva. The immunosensor showed high sensitivity, low limit of detection, and good reproducibility. It also exhibited minimal cross-reactivity with other viral antigens.
Article
Medicine, General & Internal
Julien Favresse, Constant Gillot, Maxime Oliveira, Julie Cadrobbi, Marc Elsen, Christine Eucher, Kim Laffineur, Catherine Rosseels, Sandrine Van Eeckhoudt, Jean-Baptiste Nicolas, Laure Morimont, Jean-Michel Dogne, Jonathan Douxfils
Summary: This study compared the clinical performance of five antigen detection tests for SARS-CoV-2 infection, with the VITROS automated assay showing 100% sensitivity and specificity for Ct values <= 33, outperforming rapid antigen detection tests and aligning with RT-PCR.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medical Laboratory Technology
Hannah Wang, Catherine A. Hogan, Michelle Verghese, Daniel Solis, Mamdouh Sibai, ChunHong Huang, Katharina Roltgen, Bryan A. Stevens, Fumiko Yamamoto, Malaya K. Sahoo, James Zehnder, Scott D. Boyd, Benjamin A. Pinsky
Summary: This study found that the concentration of nucleocapsid antigen in plasma is associated with the severity of COVID-19, particularly with ICU admission. Antigenemia showed comparable diagnostic performance to upper respiratory NAAT, aiding in triaging patients for optimized intensive care utilization.
CLINICAL CHEMISTRY
(2022)
Review
Medical Laboratory Technology
Xinwei Li, Mengyuan Xiong, Qiaoling Deng, Xiaobing Guo, Yirong Li
Summary: This review summarizes the utility of SARS-CoV-2 nucleocapsid protein in laboratory diagnosis. The sensitivity of SARS-CoV-2 nucleocapsid protein detection varies widely, while the specificity is generally high. The presence of N antigen is positively related to viral transmissibility, indicating that SARS-CoV-2 N protein immunoassays contribute to rapid identification and segregation of infected individuals.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Wajihul Hasan Khan, Nida Khan, Avinash Mishra, Surbhi Gupta, Vikrant Bansode, Deepa Mehta, Rahul Bhambure, M. Ahmad Ansari, Shukla Das, Anurag S. Rathore
Summary: This study demonstrates the significant impact of dimerization of the nucleocapsid protein on sensitivity of COVID-19 diagnostic assays. The dimeric form shows higher recognition by specific monoclonal antibodies and exhibits greater stability and antigenicity compared to the monomeric form. Manufacturers should maintain the monomer-dimer composition for accurate and robust diagnostics.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Virology
Sayeh Dowlatshahi, Ehsan Shabani, Mohammad J. Abdekhodaie
Summary: Coronaviruses are a family of viral pathogens that infect both birds and mammals, including humans. Seven human coronaviruses have been recognized, with three causing severe diseases. Various diagnostic techniques have been developed to detect these viruses.
ARCHIVES OF VIROLOGY
(2021)
Article
Biochemical Research Methods
Moria Barlev-Gross, Shay Weiss, Amir Ben-Shmuel, Assa Sittner, Keren Eden, Noam Mazuz, Itai Glinert, Elad Bar-David, Reut Puni, Sharon Amit, Or Kriger, Ofir Schuster, Ron Alcalay, Efi Makdasi, Eyal Epstein, Tal Noy-Porat, Ronit Rosenfeld, Hagit Achdout, Ohad Mazor, Tomer Israely, Haim Levy, Adva Mechaly
Summary: Public health experts emphasize the importance of quick, point-of-care SARS-CoV-2 detection for controlling virus spread, but concerns have been raised about the tendency of certain antigen detection devices to exhibit false positive results.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Gastroenterology & Hepatology
Weiwu Gao, Zhengcai Jia, Yi Tian, Penghui Yang, Hui Sun, Chenhui Wang, Yi Ding, Mengjie Zhang, Yi Zhang, Di Yang, Zhiqiang Tian, Jian Zhou, Zhihua Ruan, Yuzhang Wu, Bing Ni
Correction
Immunology
Jintao Li, Ji Zhang, Hongxia Guo, Shimin Yang, Weiping Fan, Nan Ye, Zhiqiang Tian, Tiantian Yu, Guoping Ai, Zigang Shen, Haiyang He, Ping Yan, Hui Lin, Xue Luo, Hongli Li, Yuzhang Wu
FRONTIERS IN IMMUNOLOGY
(2020)
Letter
Immunology
Xiangyu Chen, Ren Li, Zhiwei Pan, Chunfang Qian, Yang Yang, Renrong You, Jing Zhao, Pinghuang Liu, Leiqiong Gao, Zhirong Li, Qizhao Huang, Lifan Xu, Jianfang Tang, Qin Tian, Wei Yao, Li Hu, Xiaofeng Yan, Xinyuan Zhou, Yuzhang Wu, Kai Deng, Zheng Zhang, Zhaohui Qian, Yaokai Chen, Lilin Ye
CELLULAR & MOLECULAR IMMUNOLOGY
(2020)
Article
Immunology
Huang Huang, Xiaoping Wu, Dongwei Meng, Yizhou Feng, Lan Zhou, Zhenyu Liu, Shupei Tang, Xueqin Li, Yi Cao, Haiyang He, Zhunyi Xie, Jingbo Zhang, Yongwen Chen, Tingting Zhao, Yuzhang Wu, Xinyuan Zhou
Summary: The study demonstrated that LXR beta deficiency leads to a reduction in single-positive (SP) thymocytes and increased apoptosis, indicating the crucial role of LXR beta in promoting the survival of SP thymocytes. The findings also suggest that LXR beta positively regulates the expression of IL-7R alpha in SP thymocytes, which is essential for their survival.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Endocrinology & Metabolism
Lina Wang, Ping Sun, Yuzhang Wu, Li Wang
Summary: Obesity-induced low-grade chronic inflammation in metabolic tissues is a major factor for various diseases. Tissue-resident macrophages and adaptive CD8(+) T cells play important roles in this process, but further research is needed to understand the mechanisms involved.
Review
Biochemistry & Molecular Biology
Yiwei Zhang, Zhiyuan Wei, Hui Dong, Jian Zhou, Jizhao Yuan, Bing Ni, Yuzhang Wu, Chao Han, Yi Tian
Summary: This review summarizes the role of RNA-binding proteins, microRNAs, and long non-coding RNAs in regulating the fate of Th17 cells, as well as the impact of RNA modifications and nonsense-mediated mRNA decay in Th17 cells. Ongoing research may provide insights into practical applications for regulating mRNA stability in the context of Th17-related autoimmune diseases.
Article
Multidisciplinary Sciences
Yi Tian, Chao Han, Zhiyuan Wei, Hui Dong, Xiaohe Shen, Yiqiang Cui, Xiaolan Fu, Zhiqiang Tian, Shufeng Wang, Jian Zhou, Di Yang, Yi Sun, Jizhao Yuan, Bing Ni, Yuzhang Wu
Summary: This study identified a novel enhancer, RORCE2, for the transcription factor RORγt, which interacts with Sox5 to modulate RORγt expression at the promoter level, providing insights into the regulation of Th17 differentiation and functions.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Rongjuan Pei, Jianqi Feng, Yecheng Zhang, Hao Sun, Lian Li, Xuejie Yang, Jiangping He, Shuqi Xiao, Jin Xiong, Ying Lin, Kun Wen, Hongwei Zhou, Jiekai Chen, Zhili Rong, Xinwen Chen
Summary: Human lung organoids can model the infection and response of SARS-CoV-2 in the lungs, providing a valuable pathophysiological model for studying the virus and testing potential therapeutic drugs for COVID-19.
Article
Biochemistry & Molecular Biology
Yiwei Zhang, Jian Zhou, Zhiyuan Wei, Hui Dong, Di Yang, Yuanyu Deng, Jiahui Li, Saiyu Shi, Yi Sun, Huimin Lu, Jizhao Yuan, Bing Ni, Yuzhang Wu, Yi Tian, Chao Han
Summary: Dendritic cells play a crucial role in the immune system by regulating defense against pathogens and tolerance to self. This review highlights the significance of mRNA stability regulation in dendritic cells for their function and immune therapy.
Review
Immunology
Hui Sun, Yuzhang Wu, Yi Zhang, Bing Ni
Summary: This study focuses on the production mechanisms of IL-10 in ILCs, the key role of IL-10 in NK and ILC2 cells, and the potential impact of IL-10-producing ILCs in diseases such as infections, allergies, and cancers.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Jianhai Yu, Zhiran Qin, Xuling Liu, Xiaoen He, Jinxiu Yao, Xuan Zhou, Kun Wen, Nan Yu, Qinghua Wu, Weiwei Xiao, Li Zhu, Chengsong Wan, Bao Zhang, Wei Zhao
Summary: This study analyzed the nucleocapsid protein of the novel coronavirus and identified specific epitopes that can effectively detect antibodies with high specificity. These findings can be used for serological diagnosis and kinetic studies of SARS-CoV-2 antibody detection.
COMPUTERS IN BIOLOGY AND MEDICINE
(2022)
Article
Immunology
Yujun Pei, Zheng Xiang, Kun Wen, Chloe Ran Tu, Xiwei Wang, Yanmei Zhang, Xiaofeng Mu, Yinping Liu, Wenwei Tu
Summary: This study found that the antitumor activity of Vgamma9Vdelta2-T cells was highly suppressed by the immunosuppressive tumor microenvironment (TME) mediated by hIL-10 and vIL-10. CD137 costimulation significantly mitigated the suppressive effects of IL-10 in TME by suppressing IL-10R1 expression, and improved the compromised antitumor activity of Vgamma9Vdelta2-T cells. In humanized mice, CD137 costimulation boosted the therapeutic effects of aminobisphosphonate pamidronate against EBV-induced lymphoma.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yanyan Zhang, Baohua Li, Qiang Bai, Pengcheng Wang, Gang Wei, Zhirong Li, Li Hu, Qin Tian, Jing Zhou, Qizhao Huang, Zhiming Wang, Shuai Yue, Jialin Wu, Liuqing Yang, Xinyuan Zhou, Lubin Jiang, Ting Ni, Lilin Ye, Yuzhang Wu
Summary: The study identified a key lncRNA-Snhg1 in CD8 T cells that promotes memory formation and inhibits effector cell differentiation. Snhg1 interacts with Vps13D and regulates IL-7 signaling to play a crucial role in memory CD8 cell generation. The Snhg1-Vps13D-IL-7R-TCF1 axis is central in memory CD8 establishment and a potential target for enhancing vaccination effects against ongoing pandemics.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)