Article
Food Science & Technology
Shen Zhuang, Ke Ming, Ning Ma, Jinrui Sun, Dehai Wang, Mingxing Ding, Yi Ding
Summary: A specific polysaccharide fraction (POPZ) from Portulaca oleracea L. was found to inhibit LPS-induced barrier injury and inflammatory responses in porcine intestinal epithelial monolayer, possibly through inactivating TLR4/NF-κB pathway and activating EGF/EGFR pathway.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Review
Cell Biology
Nachiket M. Godbole, Asif Alam Chowdhury, Neha Chataut, Shanjana Awasthi
Summary: The lung epithelium plays a crucial role in responding to infections, toxins, and allergies, as well as maintaining normal pulmonary function. The tight junctions between lung epithelial cells are essential for maintaining the integrity and fluid balance of the lung. This review summarizes the literature on disrupted tight junctions and epithelial barrier in various lung conditions, and discusses the expression and regulation of specific tight junction proteins against pathogenic stimuli. The review also explores the molecular signaling and crosstalk among signaling pathways that control the tight junction structure and function in the lung.
Review
Cell Biology
Zahra Chegini, Mojtaba Didehdar, Amin Khoshbayan, Jafar Karami, Milad Yousefimashouf, Aref Shariati
Summary: Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) is a multifactorial disease, and bacterial superantigens such as Staphylococcus aureus are known to play a role in its development. Specifically, Staphylococcus aureus nasal colonization and IgE response to Staphylococcus aureus enterotoxins have been found in CRSwNP patients. Among the enterotoxins, enterotoxin B (SEB) has been identified as a key player in the pathogenesis of CRSwNP by disrupting mucosal integrity and triggering inflammatory responses. SEB activates Toll Like Receptor 2, leading to the production of pro-inflammatory cytokines, and induces cellular stress and oxidative damage. It also promotes eosinophil degranulation and localized eosinophilic inflammation. Furthermore, SEB may be involved in the regulation of immune cells and maintenance of sinonasal mucosal inflammation. While in vitro and animal studies have provided valuable insights, further research is needed to fully understand the role of SEB in CRSwNP.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Cell Biology
Yuju Kim, Yunna Lee, Gwangbeom Heo, Sihyun Jeong, Soyeong Park, Jin-Wook Yoo, Yunjin Jung, Eunok Im
Summary: The study found that PAR2-induced autophagy can maintain mucosal barrier integrity by regulating intestinal epithelial permeability, possibly through the β-arrestin-ERK1/2 pathway. Therefore, regulating PAR2 can be a suitable strategy for treating intestinal diseases.
Article
Food Science & Technology
Sefat E. Khuda, Ann V. Nguyen, Girdhari M. Sharma, Mohammad S. Alam, Kannan V. Balan, Kristina M. Williams
Summary: This study found that polysorbate-80 may enhance the absorption of allergens in a size-dependent manner by modulating paracellular permeability.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Microbiology
Yi Zhou, Ling Duan, Yan Zeng, Xu Song, Kangcheng Pan, Lili Niu, Yang Pu, Jiakun Li, Abdul Khalique, Jing Fang, Bo Jing, Dong Zeng, Bairong Shen, Xueqin Ni
Summary: The intestinal microbiota of captive pandas, characterized by a high abundance of Enterobacteriaceae, has led to intestinal infection. Probiotic supplement Lactiplantibacillus plantarum BSG201683 (L. plantarum G83) has shown effectiveness in improving intestinal barrier function and inflammation. In vitro studies demonstrated that L. plantarum G83 reduced paracellular permeability, decreased proinflammatory cytokines, and enhanced anti-inflammatory responses through Toll-like receptor 4/ nuclear factor kappa-B (TLR4/NF-?B) signaling pathways.
Article
Biochemistry & Molecular Biology
Javier Estevez, Vicente Martinez
Summary: Toll-like receptors (TLRs) play a role in microbial regulation of gastrointestinal functions, including the epithelial barrier function (EBF). This study investigated the effects of TLR7 stimulation on colonic EBF in rats. Results showed that TLR7 stimulation with imiquimod had a pro-barrier effect, while the combination with dimethyl sulfoxide (DMSO) had a detrimental effect on EBF. These effects were not associated with changes in gene expression or distribution of tight junction-related proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ashwinkumar Subramenium Ganapathy, Kushal Saha, Eric Suchanec, Vikash Singh, Aayush Verma, Gregory Yochum, Walter Koltun, Meghali Nighot, Thomas Ma, Prashant Nighot
Summary: This study revealed that increased clathrin-mediated endocytosis under starvation conditions promotes degradation of CLDN2 and enhances the TJ barrier. The association of CLDN2 with clathrin and AP2 as well as LC3 and lysosomes increased upon starvation, indicating the role of clathrin-mediated endocytosis in autophagy-induced CLDN2 degradation.
Article
Multidisciplinary Sciences
Atul K. Srivastava, Bharat Somireddy Venkata, Yan Y. Sweat, Heather R. Rizzo, Lea Jean-Francois, Li Zuo, Kathleen W. Kurgan, Patrick Moore, Nitesh Shashikanth, Izabela Smok, Joseph R. Sachleben, Jerrold R. Turner, Stephen C. Meredith
Summary: This study investigates the effects of S408 phosphorylation on occludin protein structure and demonstrates its role in regulating intercellular junction function through interactions with other components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Pharmacology & Pharmacy
Sakthikumar Ragupathy, Joel Brunner, Gerrit Borchard
Summary: A short peptide sequence L-R5 has been identified as a partial inhibitor of intracellular protein kinase C, capable of enhancing epithelial paracellular permeability in a reversible and non-toxic manner. Experimental results show that L-R5 transiently increases epithelial permeability, enhances the transport of large molecules, and is stable and effective at body temperature without cytotoxicity.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Microbiology
Ulrica Thunberg, Svante Hugosson, Ralf Ehricht, Stefan Monecke, Elke Mueller, Yang Cao, Marc Stegger, Bo Soderquist
Summary: This study analyzed Staphylococcus aureus diversity, genetic factors, and humoral immune responses in chronic rhinosinusitis (CRS) patients through long-term follow-up. The results showed that some patients retained S. aureus isolates from the same genetic lineage over a decade, but the overall changes in antibody responses to staphylococcal antigens were highly variable, with no correlation found between antigen-encoding genes and corresponding antibody levels in serum.
Article
Geriatrics & Gerontology
Kevin M. Najarro, Devin M. Boe, Travis M. Walrath, Juliet E. Mullen, Madison T. Paul, John H. Frankel, Holly J. Hulsebus, Juan-Pablo Idrovo, Rachel H. McMahan, Elizabeth J. Kovacs
Summary: The study demonstrates that advanced age exacerbates intestinal epithelial permeability after burn injury. Increased apoptosis may lead to elevated intestinal leakiness and bacterial accumulation in mesenteric lymph nodes, while S100a8/9 may serve as a biomarker for heightened inflammation in the intestine.
EXPERIMENTAL GERONTOLOGY
(2022)
Article
Medicine, Research & Experimental
Lianghui Zhan, Jingru Zheng, Jianbiao Meng, Danting Fu, Lisha Pang, Chunlian Ji
Summary: This study found that TLR4 deficiency can reduce inflammatory responses and apoptosis, improve intestinal damage, regulate intestinal flora, and alleviate LPS-induced intestinal barrier dysfunction.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Cell Biology
Lauren F. O'Leary, Andrea M. Tomko, Denis J. Dupre
Summary: The immune system plays a crucial role in regulating the permeability of the epithelial barrier and maintaining immune balance. Tolerance must be maintained for both pathogenic insults and non-pathogenic antigens, with the endocannabinoid system also contributing to this balance. Understanding the effects of endo- and phytocannabinoids on epithelial permeability could lead to potential therapeutic targets for dysregulated immune responses and diseases.
INFLAMMATION RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Rina Fujiwara-Tani, Shiori Mori, Ruiko Ogata, Rika Sasaki, Ayaka Ikemoto, Shingo Kishi, Masuo Kondoh, Hiroki Kuniyasu
Summary: CLDN4 is a crucial component of tight junctions in epithelial cells and is overexpressed in many epithelial malignancies. Changes in CLDN4 expression are associated with epigenetic factors, inflammation, and growth factor signaling. CLDN4 plays a role in maintaining the tumor microenvironment and acts as a barrier to anticancer drug entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Allergy
Sven F. Seys, Peter W. Hellings, Isam Alobid, Vibeke Backer, Emilie Bequignon, Christian von Buchwald, Carlo Cavaliere, Andre Coste, Lauren Deneyer, Zuzana Diamant, Julia Eckl-Dorna, Wytske J. Fokkens, Simon Gane, Philippe Gevaert, Christiane Holbaek-Haase, Clemens Holzmeister, Claire Hopkins, Valerie Hox, Caroline Huart, Roger Jankowski, Mark Jorissen, Anette Kjeldsen, Lisa Knipps, Bibi Lange, Rik van der Lans, Anu Laulajainen-Hongisto, Kenneth Larsen, David T. Liu, Valerie Lund, Gert Marien, Simonetta Masieri, Geoffrey Mortuaire, Joaquim Mullol, Sietze Reitsma, Philippe Rombaux, Sven Schneider, Andreas Steinsvik, Peter-Valentin Tomazic, Sanna K. Toppila-Salmi, Laura Van Gerven, Thibaut Van Zele, Paula Virkkula, Martin Wagenmann
Summary: This study aims to establish an international chronic rhinosinusitis outcome registry based on real-world data collection enabled by mobile health technology. A collaborative scientific network of 17 university ear-nose-throat clinics from 10 European countries has been established to collect data in a longitudinal and standardized manner. Up to 300 patients have already been included.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Letter
Allergy
Senne Gorris, Saartje Uyttebroek, Wout Backaert, Mark Jorissen, Rik Schrijvers, Mark J. Thompson, Dirk Loeckx, Sven F. Seys, Laura Van Gerven, Peter W. Hellings
Review
Biochemistry & Molecular Biology
Eva Ganseman, Mieke Gouwy, Dominique M. A. Bullens, Christine Breynaert, Rik Schrijvers, Paul Proost
Summary: A significant proportion of adult-onset asthma is caused by occupational exposure to various agents. Insect allergies can occur in several professions, with respiratory symptoms such as asthma being the most common. The recent approval of insects for human consumption by the European Union may increase the risk of occupational insect allergies. This review highlights the need for better diagnostics and primary prevention measures to protect workers in the developing insect industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dermatology
Dorien Bamps, Katarina Berdon, Hasan Hernandez, Rik Schrijvers, Jan de Hoon
Summary: This study investigated the measurement method of histamine-induced skin blood flow and flare area, as well as the effects of certain drugs on histamine-induced blood flow and flare. The results showed inconsistent correlation between ruler and laser Doppler imaging assessments of the flare area, and the reproducibility and histamine-dependency of the measurements were not obvious. The study suggested that a lower dose of histamine might be more suitable as a quantitative biomarker.
SKIN PHARMACOLOGY AND PHYSIOLOGY
(2023)
Article
Allergy
Johannes Luebke, Juliana Schwaab, Deborah Christen, Hanneke Oude Elberink, Bart Span, Marek Niedoszytko, Aleksandra Gorska, Magdalena Lange, Karoline V. Gleixner, Emir Hadzijusufovic, Oleksii Solomianyi, Irena Angelova-Fischer, Roberta Zanotti, Massimiliano Bonifacio, Patrizia Bonadonna, Khalid Shoumariyeh, Nikolas von Bubnoff, Sabine Mueller, Cecelia Perkins, Chiara Elena, Luca Malcovati, Hans Hagglund, Mattias Mattsson, Roberta Parente, Judit Varkonyi, Anna Belloni Fortina, Francesca Caroppo, Alexander Zink, Knut Brockow, Christine Breynaert, Dominique Bullens, Akif Selim Yavuz, Michael Doubek, Vito Sabato, Tanja Schug, Dietger Niederwieser, Karin Hartmann, Massimo Triggiani, Jason Gotlib, Olivier Hermine, Michel Arock, Hanneke C. Kluin-Nelemans, Jens Panse, Wolfgang R. Sperr, Peter Valent, Andreas Reiter, Mohamad Jawhar
Summary: Organomegaly, including splenomegaly, hepatomegaly, and/or lymphadenopathy, is an important diagnostic and prognostic feature in patients with cutaneous mastocytosis (CM) or systemic mastocytosis (SM). The number of organomegalies is adversely associated with overall survival (OS), and lymphadenopathy is frequently detected in patients with SM, particularly in advanced SM (AdvSM). The presence of lymphadenopathy in AdvSM is associated with inferior outcomes compared to AdvSM patients without lymphadenopathy.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Letter
Allergy
Janne Goossens, Josefien Vandekerckhove, Anne-Charlotte Jonckheere, Ellen Dilissen, Sven F. Seys, Vincent Vanbelle, Sven Aertgeerts, Jeroen Stappers, Koen Peers, Marc Raes, Sophie Verelst, Jasmine Leus, Lieven J. Dupont, Dominique M. A. Bullens
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
D. G. Ebo, M. M. van der Poorten, A. L. Van Gasse, R. Schrijvers, C. Hermans, M. Bartiaux, F. Haerynck, J. Willekens, H. Lapeere, M. Moutschen, O. Michel, V. Sabato
Summary: This paper assessed the alignment of Belgian clinical practice in hereditary angioedema (HAE) with the updated international guideline and identified opportunities for optimization. The main action points to further optimize the Belgian clinical practice of HAE include working towards total disease control, informing patients about new long-term prophylactic therapies, ensuring availability of on-demand therapy, implementing a more comprehensive assessment in daily clinical practice, and expanding the existing patient registry.
ACTA CLINICA BELGICA
(2023)
Article
Immunology
Mariia Dzhus, Lisa Ehlers, Marjon Wouters, Katrien Jansen, Rik Schrijvers, Lien De Somer, Steven Vanderschueren, Marco Baggio, Leen Moens, Benjamin Verhaaren, Rik Lories, Giorgia Bucciol, Isabelle Meyts
Summary: Deficiency of human adenosine deaminase type 2 (DADA2) is a rare autoimmune disorder characterized by vasculopathy, immune dysregulation, and hematologic abnormalities. Neurological manifestations, particularly strokes, are a significant feature of DADA2, with brain stem and deep gray matter being commonly affected. Other neurological involvements include neuropathies, focal deficits, ophthalmological findings, convulsions, and headaches. Recognizing the diverse neurological manifestations of DADA2 is crucial for accurate diagnosis and appropriate management.
JOURNAL OF CLINICAL IMMUNOLOGY
(2023)
Article
Allergy
Toon Ieven, Lieve Coorevits, Martijn Vandebotermet, Sebastiaan Tuyls, Helene Vanneste, Lisa Santy, Dries Wets, Paul Proost, Glynis Frans, David Devolder, Christine Breynaert, Dominique M. A. Bullens, Rik Schrijvers
Summary: This study evaluated the tolerance of PEGylated lipid nanoparticle vaccine (BNT162b2) in patients with PEG and/or PS80 allergy and explored the mechanism of reactivity. The results showed that all PEG and/or PS80 allergic patients can safely receive SARS-CoV-2 vaccines.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Meeting Abstract
Gastroenterology & Hepatology
I. Jacobs, S. Deleu, J. Cremer, G. De Hertogh, S. Vermeire, C. Breynaert, T. Vanuytsel, B. Verstockt
JOURNAL OF CROHNS & COLITIS
(2023)
Meeting Abstract
Gastroenterology & Hepatology
I. Jacobs, B. J. Ke, J. Cremer, A. D'Hoore, G. Bislenghi, G. Matteoli, G. De Hertogh, J. Sabino, M. Ferrante, S. Vermeire, C. Breynaert, T. Vanuytsel, B. Verstockt
JOURNAL OF CROHNS & COLITIS
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Inge Jacobs, Sara Deleu, Jonathan Cremer, Gert De Hertogh, Severine Vermeire, Christine M. Breynaert, Bram Verstockt, Tim Vanuytsel