期刊
CHEMICAL ENGINEERING JOURNAL
卷 397, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2020.125348
关键词
Hydraulic fracturing wastewater; Enhanced bioremediation; Acinetobacter; Biosurfactant; Indigenous microorganisms; Functional genes
资金
- National Natural Science Foundation of China [41977269, 51978189]
- Fundamental Research Funds for the Central Universities
- Open Foundation from Fishery Sciences in the First-Class Subjects of Zhejiang [20190007, 20190009]
- Natural Science Foundation of Guangxi Province [2018GXNSFAA138202]
- National Key Research and Development Project [2018YFD0800704]
In this study, a biosurfactant-producing strain Y2 was isolated from hydraulic fracturing flowback and produced water (HF-FPW) and identified as Acinetobacter sp. The biosurfactant produced by Y2 could reduce surface tension of water from 72.2 mN/m to 30.2 mN/m with a CMC value of 187.5 mg/L and showed strong tolerance against wide ranges of pH (2-12), temperatures (4-100 degrees C), and salinity (0-100 g/L). Through thin layer chromatography, Fourier transform infrared spectroscopy, and gas chromatography-mass spectrometry analysis, the biosurfactant was characterized as lipopeptide. Inoculation of Acinetobacter sp. Y2 into HF-FPW greatly increased the activity and growth of the microcosm. Meanwhile, it significantly (P < 0.05) promoted the removal of chemical oxygen demand (reduction from 6646.7 mg/L to 1546.7 mg/L), and the degradation of n-alkanes (reduction from 2635.4 mg/L to 159.7 mg/L) and polycyclic aromatic hydrocarbons (reduction from 918.6 mu g/L to 209.6 mu g/L) in 7 days. High-throughput sequencing analysis revealed that the inoculation of indigenous biosurfactant producer modified the bacterial community structure and enriched the hydrocarbon-degrading microorganisms including Pseudomonas sp. and Rhizobium sp. The real-time quantitative PCR results showed that the expression of several key functional genes was greatly enhanced by Y2 addition and was significantly correlated with Acinetobacter sp. This study proved the introduction of native biosurfactant-producing microorganisms as a promising in situ strategy for HF-FPW bioremediation and the underlying mechanisms were proposed.
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