Article
Biotechnology & Applied Microbiology
Tianlei Zheng, Yan Li, Xiaozai Zhang, Jia Xu, Ming Luo
Summary: This study investigated whether exosomes derived from synovial mesenchymal stem cells (SMSCs) overexpressing miR-212-5p could be used to treat degenerative chondrocytes. The results showed that miR-212-5p was down-regulated while E74 Like ETS Transcription Factor 3 (ELF3) was up-regulated in osteoarthritis synovial tissues, and they had a negative correlation. Further experiments demonstrated that miR-212-5p directly targeted ELF3 and regulated its expression in SMSCs. Additionally, it was found that SMSC-212-5p-Exos could suppress chondrocyte degeneration and inflammation by inhibiting the up-regulation of ELF3, degeneration molecules, matrix metalloproteinase, and inflammatory molecules.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Qingju Li, Yinchuan Xu, Kaiqi Lv, Yingchao Wang, Zhiwei Zhong, Changchen Xiao, Keyang Zhu, Cheng Ni, Kan Wang, Minjian Kong, Xuebiao Li, Youqi Fan, Fengjiang Zhang, Qi Chen, Yi Li, Qian Li, Chengjia Liu, Jinyun Zhu, Shuhan Zhong, Jingyi Wang, Yongjian Chen, Jing Zhao, Dan Zhu, Rongrong Wu, Jinghai Chen, Wei Zhu, Hong Yu, Reza Ardehali, Jianyi (Jay) Zhang, Jian'an Wang, Xinyang Hu
Summary: This study highlights the key role of sEV miR-486-5p in promoting cardiac angiogenesis. miR-486-5p acts on the fibroblastic MMP19-VEGFA signaling pathway to promote cardiac recovery. Delivery of miR-486-5p-engineered sEVs safely enhanced angiogenesis and cardiac function in a nonhuman primate (NHP) MI model, potentially promoting cardiac repair.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Qingqing Xia, Quan Wang, Feng Lin, Junjuan Wang
Summary: The study revealed that miR-125a-5p can regulate chondrocyte migration and inhibit cartilage degradation, showing a negative correlation in traumatic OA. Exosomes derived from BMSCs are rich in miR-125a-5p, promoting internalization by chondrocytes and alleviating extracellular matrix degradation. These findings suggest that transferring exosomal miR-125a-5p could be a potential strategy for OA treatment.
Article
Chemistry, Medicinal
Ruina Kong, Jie Gao, Ju Zhang, Lianmei Ji, Yiyi Yu, Lanling Zhang, Dongbao Zhao
Summary: The study revealed that exosomal miR-320c derived from SMSC enhances chondrogenesis by targeting ADAM19, suggesting a potential novel mechanism for treating osteoarthritis.
FUTURE MEDICINAL CHEMISTRY
(2022)
Article
Oncology
Jin Wen, Ying Chen, Chenxi Liao, Xiao Ma, Mengyuan Wang, Qian Li, Di Wang, Yingnan Li, Xiaolan Zhang, Lei Li, Hao Zhou, Jing Zou, Lingbo Liu, Danyue Peng
Summary: This study successfully designed an effective delivery system by modifying mesenchymal stem cells (MSCs) and MSC-derived exosomes, promoting the selective clearance of LSCs and impeding AML development. These findings provide new insights into the development of novel therapies with greater cellular targeting specificity.
Article
Cell & Tissue Engineering
Yuyan Xiong, Ruijie Tang, Junyan Xu, Wenyang Jiang, Zhaoting Gong, Lili Zhang, Yu Ning, Peisen Huang, Jun Xu, Guihao Chen, Xiaosong Li, Mengjin Hu, Jing Xu, Chunxiao Wu, Chen Jin, Xiangdong Li, Haiyan Qian, Yuejin Yang
Summary: This study found that pretreatment of mesenchymal stem cells (MSCs) with the Chinese medicine Tongxinluo (TXL) can enhance cardiac repair in acute myocardial infarction (AMI) by transferring miR-146a-5p through exosomes to target the IRAK1/NF-kappa B p65 pathway. This has significant potential for clinical translation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, Research & Experimental
Piyush Gondaliya, Adil Ali Sayyed, Palak Bhat, Mukund Mali, Neha Arya, Amit Khairnar, Kiran Kalia
Summary: Diabetic wounds are a debilitating complication affecting a significant number of patients, and current therapies are unsatisfactory. This study demonstrates the potential of miR-155 inhibitor-loaded MSC-derived exosomes in promoting diabetic wound healing through synergistic effects. The study also suggests a new approach of using MSC-derived exosomes to encapsulate miRNA and antibiotics for improved management of diabetic wounds.
MOLECULAR PHARMACEUTICS
(2022)
Article
Medicine, Research & Experimental
Min Qiu, Da Liu, Qin Fu
Summary: The study found that miR-129-5p was decreased and HMGB1 was increased in OA patients and IL-1 beta-induced chondrocytes. HS-MSC-Exo rich in miR-129-5p significantly reduced inflammation and apoptosis of chondrocytes, while HS-MSC-Exo deficient in miR-129-5p increased inflammation and apoptosis. MiR-129-5p targets HMGB1 to inhibit IL-1 beta-mediated OA.
Article
Biochemistry & Molecular Biology
Hui Cheng, Jie Ding, Gusheng Tang, Aijie Huang, Lei Gao, Jianmin Yang, Li Chen
Summary: TRIM14 was significantly increased in AML patients and cell lines, with its inhibition reducing cell proliferation and inducing apoptosis through activating the PI3K/AKT pathway. HMSC-exos suppressed AML cell proliferation by delivering miR-23b-5p, which inhibited TRIM14 transcription by binding to its 3'UTR region. These findings suggest that miR-23b-5p and TRIM14 could serve as potential targets for AML treatment.
MOLECULAR MEDICINE
(2021)
Article
Engineering, Biomedical
Zhaowei Yin, Chaoren Qin, Shaowei Pan, Chen Shi, Guanfu Wu, Yan Feng, Jing Zhang, Ziyi Yu, Bin Liang, Jianchao Gui
Summary: This study compared the therapeutic effects of exosomes derived from different sources of stem cells in osteoarthritis (OA) treatment. The results showed that exosomes derived from infrapatellar fat pad (IPFP) stem cells had a significantly stronger inhibitory effect on the degradation of cartilage extracellular matrix (ECM) compared to those derived from subcutaneous adipose tissue (ScAT) stem cells. Furthermore, microRNA sequencing revealed differences in microRNA expression between the two types of exosomes, and overexpression of a specific microRNA in ScAT-derived exosomes demonstrated efficacy in inhibiting the expression of ADAMTS4, promoting ECM repair in OA. Additionally, a hyaluronan-based hydrogel microparticles (HMPs) encapsulating exosomes were fabricated using microfluidic technology, and their injectability, sustained release, and long-term therapeutic effects on OA were validated.
MATERIALS TODAY BIO
(2023)
Article
Medicine, Research & Experimental
Jing-Yuan Cao, Bin Wang, Tao-Tao Tang, Yi Wen, Zuo-Lin Li, Song-Tao Feng, Min Wu, Dan Liu, Di Yin, Kun-Ling Ma, Ri-Ning Tang, Qiu-Li Wu, Hui-Yao Lan, Lin-Li Lv, Bi-Cheng Liu
Summary: In this study, MSC-exos were found to preferentially target injured kidneys and localize to proximal tubules in ischemic AKI. The researchers demonstrated that MSC-exos ameliorated ischemic AKI and promoted tubular repair by targeting the cell cycle arrest and apoptosis of TECs through the miR-125b-5p/p53 pathway. This study provides new insights into the role of MSC-exos in renal tubule repair and highlights their potential as a promising therapeutic strategy for AKI.
Article
Medicine, General & Internal
Binya Hu, Min Huang, Lihua Tao, Yun Li, Yuting Kuang, Guangliang Liu, Sijun Zhao
Summary: This study aimed to investigate the role of exosomal miR-653-5p derived from Mesenchymal Stem Cells (MSCs) in Laryngeal Papilloma (LP). It was found that miR-653-5p was downregulated in LP tissues and cells, and it suppressed the proliferation, migration, invasion, and apoptosis of LP cells. Exosomal miR-653-5p derived from MSCs inhibited LP progression through targeting BZW2.
Article
Cell & Tissue Engineering
Jiateng Hu, Yihong Jiang, Xiaoyu Wu, Zhaoyu Wu, Jinbao Qin, Zhen Zhao, Bo Li, Zhijue Xu, Xinwu Lu, Xin Wang, Xiaobing Liu
Summary: ADSC-exos miR-17-5p regulates AAA progression and inflammation via the TXNIP-NLRP3 signaling pathway, providing a novel insight in AAA treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Anatomy & Morphology
Li Hua, Xiaohong Zhang
Summary: The study revealed that MALAT1 modulated differentiation of human periodontal ligament stem cells by regulating miR155-5p during osteogenic differentiation.
Article
Cell & Tissue Engineering
Jingwen Wang, Jiale Wang, Ying Wang, Ruiyang Ma, Shucong Zhang, Jin Zheng, Wujun Xue, Xiaoming Ding
Summary: We demonstrated that bone marrow mesenchymal stem cells (BMSCs) can deliver exosomes containing miR-21-5p into islet cells. Overexpression of miR-21-5p reduced apoptosis in islets and rat insulinoma (INS-1) cells, while inhibition of miR-21-5p significantly promoted apoptosis. The upregulated miR-21-5p directly binds to the 3'UTR of programmed cell death 4 (PDCD4), thereby reducing apoptosis in transplanted islets. This study suggests that miR-21-5p can be used as a therapeutic agent in islet transplantation to minimize beta-cell apoptosis and improve transplantation outcome.
Article
Cell Biology
Chengcheng Feng, Yajun Li, Minhui Gu, Wenming Li, Yunshang Yang, Shuangshuang Chen, Yong Ma, Dechun Geng, Long Xiao, Zhirong Wang
Summary: Research has found that upregulating dopamine first receptors through activation of the Wnt/β-catenin pathway can improve osteogenesis and significantly reduce titanium particle-induced osteogenesis inhibition, making it a potential target to promote bone formation and resist prosthetic joint failure.
MEDIATORS OF INFLAMMATION
(2023)
Article
Engineering, Biomedical
Siming Zhang, Gaoran Ge, Yi Qin, Wenhao Li, Jiale Dong, Jiawei Mei, Ruixiang Ma, Xianzuo Zhang, Jiaxiang Bai, Chen Zhu, Weiwei Zhang, Dechun Geng
Summary: Poor wound healing after diabetes mellitus is characterized by persistent bleeding, inflammation dysregulation, blocked cell proliferation, infection susceptibility, and impaired tissue remodeling. Conventional wound dressings have limited functionality and fail to meet the requirements of diabetic wound healing. Stimuli-responsive hydrogels, which can change their properties in response to various stimuli, have shown promise in improving therapeutic efficacy and reducing toxicity for diabetic wound healing.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Multidisciplinary
Kai Zheng, Jiaxiang Bai, Wanling Chen, Yaozeng Xu, Huilin Yang, Wei Li, Penghui Li, Liping Tong, Huaiyu Wang, Paul K. Chu, Dechun Geng
Summary: A multifunctional therapeutic nanoplatform is designed to promote fracture healing under osteoporotic conditions. This nanoplatform, consisting of poly (lactic-co-glycolic acid) (PLGA) functionalized with alendronate (ALE) and black phosphorus nanosheets (BPs), encapsulated melatonin (MT) molecules, and named BPs/MT@PLGA-ALE nanospheres, combines the benefits of photothermal effects, suppression of osteoclastogenesis, and promotion of osteogenesis. The inclusion of ALE also enhances the bone-targeting capacity of the nanoplatform, resulting in improved therapeutic effects. This study reveals a promising strategy to treat osteoporotic fracture and expands the applications of nanomaterials in the biomedical field.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Yi Qin, Gaoran Ge, Peng Yang, Liangliang Wang, Yusen Qiao, Guoqing Pan, Huilin Yang, Jiaxiang Bai, Wenguo Cui, Dechun Geng
Summary: In the past decade, adipose-derived stem cells (ADSCs) have gained increasing attention in the field of regenerative medicine due to their accessibility, multipotency, and active paracrine activity. This review discusses the challenges in translating ADSC-based therapies to clinical settings and proposes novel strategies to overcome the limitations. It also provides an update on the advancements in ADSC-based therapies in various fields of regenerative medicine.
Review
Engineering, Biomedical
Kai Zheng, Jiaxiang Bai, Huilin Yang, Yaozeng Xu, Guoqing Pan, Huaiyu Wang, Dechun Geng
Summary: This review summarizes the current advanced nanomaterials for the diagnosis and therapy of bone-related diseases. First, the potential utility of nanomaterials for biological imaging and biomarker detection is discussed. Second, the special functions of nanomaterials as therapeutic delivery platforms for bone homeostasis regulation and cellular modulation are highlighted. Finally, perspectives in this field are offered, including current key bottlenecks and future directions, which may help in exploiting nanomaterials with novel properties and unique functions. This review provides scientific guidance to enhance the development of advanced nanomaterials for the diagnosis and therapy of bone-related diseases.
BIOACTIVE MATERIALS
(2023)
Review
Endocrinology & Metabolism
Yuhu Zhao, Xiaole Peng, Qing Wang, Zhiyu Zhang, Liangliang Wang, Yaozeng Xu, Huilin Yang, Jiaxiang Bai, Dechun Geng
Summary: The homeostasis of bone microenvironment is essential for bone health, involving both osteoblasts' bone formation and osteoclasts' bone resorption. In recent years, leptin, a hormone derived from adipocytes, has been found to affect bone homeostasis through the hypothalamic relay and the sympathetic nervous system, involving neurotransmitters like serotonin and norepinephrine. This discovery provides a new perspective on the synergistic effects of the endocrine and nervous systems on skeletal homeostasis.
Article
Cell Biology
Yu Xia, Gaoran Ge, Haixiang Xiao, Mingzhou Wu, Tianhao Wang, Chengyong Gu, Huilin Yang, Dechun Geng
Summary: This research provides evidence for the key role of REPIN1 in iron metabolism-related osteoporosis. The study demonstrates that sh-Repin1 therapy can rescue bone loss in an iron-overload-induced osteoporosis mouse model, and reveals the mechanism of REPIN1 promoting apoptosis by regulating iron metabolism.
CELL DEATH & DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Hao Xu, Wentao Wang, Xin Liu, Wei Huang, Chen Zhu, Yaozeng Xu, Huilin Yang, Jiaxiang Bai, Dechun Geng
Summary: Since the proposal of Paul Ehrlich's magic bullet concept over 100 years ago, tremendous advances have occurred in targeted therapy. Bone-targeted therapy has been considered a promising therapeutic approach for handling the challenges in drug therapy for skeletal diseases. Recent advances in therapeutic strategies based on bone targeting, including targeting strategies based on bone structure and remodeling biology, as well as bone-targeted therapeutic agents and drug delivery strategies, are discussed in this review.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Review
Cell & Tissue Engineering
Xiaole Peng, Qing Wang, Wenming Li, Gaoran Ge, Jiachen Peng, Yaozeng Xu, Huilin Yang, Jiaxiang Bai, Dechun Geng
Summary: MicroRNAs (miRNAs) are a class of endogenous single-stranded short noncoding RNAs that play vital roles in epigenetic regulation of pathological and physiological processes in animals. They fine-tune the expression of multiple genes posttranscriptionally, directing fundamental cellular pathways. miRNAs have been implicated in the onset and development of rheumatoid arthritis (RA), a chronic inflammatory disease characterized by complex pathogenesis and high morbidity, disability, and mortality rates. Understanding the deregulated miRNAs and impaired cellular functions in RA is crucial for improving clinical management and identifying potential therapeutic targets. This review comprehensively discusses the involvement of miRNAs in excessive inflammation, synovial hyperplasia, progressive joint damage, and the clinical potential of extracellular miRNAs as diagnostic and predictive biomarkers in RA.
Article
Chemistry, Multidisciplinary
Yusen Qiao, Lei Yu, Peng Yang, Miao Chen, Haifu Sun, Lingjie Wang, Bangzhao Wu, Chun-do Oh, Huilin Yang, Jiaxiang Bai, Dechun Geng
Summary: A composite material called PCLA has been developed to regulate immune response in high-glucose inflammatory environments and promote bone regeneration and angiogenesis, which is of great significance for the healing of bone wounds in diabetic patients.
