期刊
CARBOHYDRATE POLYMERS
卷 246, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.116645
关键词
Alginate; Carbohydrate-binding protein; Carbohydrate-binding module; CoMPP; Visualization
资金
- National Natural Science Foundation of China [31671883]
- Fok Ying-Tong Education Foundation [171024]
- Fundamental Research Funds for the Central Universities [201941005]
Alginate is a commercially important polysaccharide widely applied in various industries. Carbohydrate-binding proteins could be utilized as desirable tools in the investigation and further applications of polysaccharides. Few alginate-binding proteins have hitherto been characterized and reported. In the present study, a novel alginate-binding protein ABP_Wf, consisting of two orphan carbohydrate-binding modules, was cloned from a predicted alginate utilization locus of marine bacterium Wenyingzhuangia funcanilytica, and expressed in Escherichia coli. ABP_Wf exhibited a specific binding capacity to alginate, and the association constant (K-a) and affinity (K-D) were 1.94 x 10(3) M(-1)s(-1) and 1.16 x 10(-6) M. It was confirmed that the binding capacity of ABP_Wf to alginate is attributed to its constituent CBM16 domain rather than the CBM44 domain. The potentials of ABP_Wf in the semi-quantitative detection and the in situ visualization of alginate were evaluated, which implied that ABP_Wf could be served as a promising tool for investigating alginate.
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