期刊
BMC GENOMICS
卷 16, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12864-015-1725-8
关键词
Metabolic syndrome; Proteomics; Inflammation; Oxidative stress; DNA damage
资金
- European Union [LIPGENE European Integrated Project] [505944]
- Ministerio de Ciencia e Innovacion [AGL2004-07907, AGL2006-01979, AGL2009-12270]
- CIBER Fisiopatologia de la Obesidad y Nutricion [CB06/03/0047]
- Consejeria de Innovacion, Ciencia y Empresa, Junta de Andalucia [P06-CTS-01425, CTS-03039]
- Consejeria de Salud, Junta de Andalucia [06/128, 07/43, PI-0193]
- Fondo Europeo de Desarrollo Regional (FEDER)
Background: Metabolic syndrome is a multi-component disorder associated to a high risk of cardiovascular disease. Its etiology is the result of a complex interaction between genetic and environmental factors, including dietary habits. We aimed to identify the target proteins modulated by the long-term consumption of four diets differing in the quality and quantity of lipids in the whole proteome of peripheral blood mononuclear cells (PBMC). Results: A randomized, controlled trial conducted within the LIPGENE study assigned 24 MetS patients for 12 weeks each to 1 of 4 diets: a) high-saturated fatty acid (HSFA), b) high monounsaturated fatty acid (HMUFA), c) low-fat, high-complex carbohydrate diets supplemented with placebo (LFHCC) and d) low-fat, high-complex carbohydrate diets supplemented with long chain (LC) n-3 polyunsaturated fatty acids (PUFA) (LFHCC n-3). We analyzed the changes induced in the proteome of both nuclear and cytoplasmic fractions of PBMC using 2-D proteomic analysis. Sixty-seven proteins were differentially expressed after the long-term consumption of the four diets. The HSFA diet induced the expression of proteins responding to oxidative stress, degradation of ubiquitinated proteins and DNA repair. However, HMUFA, LFHCC and LFHCC n-3 diets down-regulated pro-inflammatory and oxidative stress-related proteins and DNA repairing proteins. Conclusion: The long-term consumption of HSFA, compared to HMUFA, LFHCC and LFHCC n-3, seems to increase the cardiovascular disease (CVD) risk factors associated with metabolic syndrome, such as inflammation and oxidative stress, and seem lead to DNA damage as a consequence of high oxidative stress.
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