期刊
BIOTECHNIQUES
卷 69, 期 4, 页码 317-325出版社
FUTURE SCI LTD
DOI: 10.2144/btn-2020-0057
关键词
COVID-19; digital PCR; microfluidics; PCR; point-of-care diagnostics; portable systems; reverse transcription PCR; RNA virus diagnoses
资金
- PR China [W099109]
- GACR from the Czech Republic [GA16-11140S]
- Ministry of Education, Youth and Sport of the Czech Republic [LTACH19005]
- Ministry of Science and Technology, PR China [2018YFE010900]
PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.
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