Article
Chemistry, Analytical
Maria M. P. Melo, Ana Machado, Antonio O. S. S. Rangel, Raquel B. R. Mesquita
Summary: A new microfluidic paper-based analytical device (muPAD) was developed for accurate creatinine quantification in urine samples. The paper device is accessible, portable, low-cost, and environmentally friendly. It uses a colorimetric reaction and digital image scanning for quantification and achieves similar results to traditional batch-wise procedures.
Article
Chemistry, Applied
Ana Carolina Rafanhin Sousa, Cleyton Nascimento Makara, Lais Canniatti Brazaca, Emanuel Carrilho
Summary: In order to detect sulfonamides in milk, a simple and portable microfluidic paper-based analytical device (mu PAD) was developed for precise analysis without the need for pretreatment. This device has good linearity and selectivity, potentially allowing for on-site monitoring of milk quality to reduce adverse conditions associated with high concentrations of sulfonamides.
Article
Chemistry, Analytical
Ahmed A. Shalaby, Chia-Wen Tsao, Akihiko Ishida, Masatoshi Maeki, Manabu Tokeshi
Summary: Cancer is a leading cause of death worldwide, and early diagnosis is crucial for successful treatment. In developing countries, there is a need for low-cost, accurate, and easy to use point-of-need (PON) devices for cancer diagnosis and treatment follow-up. Paper-based analytical devices (PADs) are considered a key solution as they provide a low-cost platform for developing PON biosensors. This review discusses the various factors involved in the development of PADs for cancer biomarker detection and summarizes the advantages and disadvantages of each method.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Inmaculada Ortiz-Gomez, Almudena Rivadeneyra, Jose F. Salmeron, Ignacio de Orbe-Paya, Diego P. Morales, Luis Fermin Capitan-Vallvey, Alfonso Salinas-Castillo
Summary: In this study, a microfluidic paper-based analytical device (mu PAD) with an NFC tag was proposed as a portable, simple and fast colorimetric method for glutathione (GSH) determination. The method took advantage of the oxidizing property of Ag+ on 3,3';,5,5'-tetramethylbenzidine (TMB), which resulted in a blue color fading when GSH was present. By integrating electronic interfaces into the hardware of digital image capture, a smartphone was utilized for the colorimetric determination of GSH. Importantly, this new method exhibited a low detection limit of 1.0 mu M, offering high sensitivity and a simple, fast, portable and low-cost GSH determination within 20 minutes using a colorimetric signal.
Article
Chemistry, Analytical
Farshad Ardakani, Zahra Shojaeifard, Bahram Hemmateenejad
Summary: We present a three-dimensional origami paper-based analytical device (3D-muPAD) for ABO and Rh blood type detection using a three-dimensional origami microfluidic technology. By counting the number of paper layers through which blood moves in antibody channels, the blood type can be accurately determined. This device offers higher accuracy and efficiency compared to traditional methods.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Pavar Sai Kumar, Sunil Bhand, Ashis Kumar Das, Sanket Goel
Summary: A novel CLSA approach on a 3D printed microfluidic paper-based analytical device was demonstrated, with the working temperature directly influencing the signal intensity and achieving stable CL signal amplification. The method showed strong potential for multiple CL-based detection and monitoring applications.
Article
Chemistry, Analytical
Raquel Catalan-Carrio, Tugce Akyazi, Lourdes Basabe-Desmonts, Fernando Benito-Lopez
Summary: The main challenge for the expansion and mass production of microfluidic paper-based analytical devices lies in the lack of fluid flow control due to uncontrolled fabrication protocols. The key to addressing this issue is to generate uniform and reliable microfluidic channels and optimize the fabrication process. Wax printing is the most common method, which requires analysis and optimization of parameters such as device shape, wax barrier width, and internal area.
Article
Biochemical Research Methods
Shaolong Feng, Marti Z. Hua, M. S. Roopesh, Xiaonan Lu
Summary: We have developed a competitive ELISA-based origami microfluidic paper-based analytical device (mu PAD) for the detection of mycotoxins in animal feed materials. The mu PAD was patterned using the wax printing technique, with a testing pad in the middle and absorption pads on the sides. By immobilizing anti-mycotoxin antibodies on chitosan-glutaraldehyde-modified sample reservoirs, we successfully achieved the quantification of zearalenone, deoxynivalenol, and T-2 toxin in corn flour in just 20 minutes. The mu PAD integrated with competitive ELISA shows potential for rapid, sensitive, and cost-effective detection of various mycotoxins in animal feed materials, which is important for the livestock industry.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Li-Xia Feng, Chao Tang, Xiao-Xuan Han, Hui-Chao Zhang, Feng-Na Guo, Ting Yang, Jian-Hua Wang
Summary: In this study, ZnO nanorods were hydrothermally grown on Whatman filter paper to fabricate a microfluidic paper-based device for simultaneous detection of glucose and uric acid. The presence of ZnO NRs promoted enzyme immobilization and overcame the coffee ring effect through uniform distribution and specialized design. Two color indicators provided complementary results for quantifying target analytes visually.
Article
Chemistry, Analytical
Bao Thai Tran, Patsamon Rijiravanich, Nitipon Puttaraksa, Werasak Surareungchai
Summary: A hydrophobic wax barrier combined with surfactants was developed as a valving mechanism in paper-based microfluidic systems to control delays in reagent addition. The wax gate allowed the delay of reagent delivery and assisted multistep analysis. The integration of the triangle wax gate into prototype immunosensors enabled sensitive detection of mouse-IgG and SARS-CoV2 antibody.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Jie Zheng, Min Zhu, Jiao Kong, Zimu Li, Jianming Jiang, Yachao Xi, Fang Li
Summary: A simple microfluidic paper-based analytical device using platinum nanoparticles as peroxidase mimic was developed for simultaneous determination of glucose and uric acid. The multi-layered structure improves color uniformity and intensity, leading to enhanced sensitivity in detection. The device showed satisfactory results in real serum samples and integrated with smartphone for easy and intelligent detection, making it suitable for simple and portable on-site analysis.
Article
Chemistry, Analytical
Mason E. Bonacci, M. Ines G. S. Almeida, Yanlin Zhang, Spas D. Kolev
Summary: This study developed a novel microfluidic paper-based analytical device (μPAD) for the speciation of inorganic arsenic in environmental aqueous samples. The device showed advantages of simplicity, high sensitivity, and low detection limit.
Review
Chemistry, Medicinal
Atchara Lomae, Pattarachaya Preechakasedkit, Kanyapat Teekayupak, Yosita Panraksa, Jutiporn Yukird, Orawon Chailapakul, Nipapan Ruecha
Summary: This article comprehensively reviews the fabrication methods and device designs of microfluidic paper-based analytical devices (mu PADs), focusing on their applications in motif identification. The advantages and disadvantages of different techniques and designs are compared, and various powerful detection methods are integrated into mu PADs for medicinal diagnosis. A great prototype for real-life application breakthrough is also discussed.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Pornchanok Sawetwong, Sanoe Chairam, Purim Jarujamrus, Maliwan Amatatongchai
Summary: A novel three-dimensional microfluidic paper-based analytical device (3D-muPAD) with colorimetric detection using Mn-ZnS quantum dot embedded molecularly imprinted polymer (Mn-ZnS QD-MIP) was developed for selective glyphosate determination in whole grain samples. The device showed high accuracy, good reproducibility, and promising applications in food-quality control and onsite environmental monitoring.
Article
Chemistry, Analytical
Yunlong Nie, Fang Zhou, Chenye Wang
Summary: A simple paper-based microfluidic device was fabricated to detect multiple targets simultaneously. The device consists of a single-layer moving sliding PAD (SPAD) and a folding origami PAD (OPAD) to control the flow channel switch and test the target analytes, respectively. The device successfully determined the concentration of Fe(III), Ni(II), Cr(VI), and nitrite in standard and actual solutions, with a low limit of detection (LOD). The 3D PAD holds great promise in environmental monitoring, biochemical analysis, food testing, and other industries.
Article
Chemistry, Analytical
Ping Li, Hao Fu, Zhenyu Bai, Xiaoyang Feng, Ji Qi, Xingliang Song, Xueping Hu, Lingxin Chen
Summary: An effective strategy is proposed to construct a highly sensitive ratiometric fluorescence sensing platform for microcystins (MCs) based on a dummy molecularly imprinted polymer using metformin as a template. The imprinted nanohybrids of carbon dots (CDs) combined with fluorescein isothiocyanate (FITC) are synthesized (CDs-FITC-SiO2@MIP), in which the CDs and FITC serve as assisted response signals and reference enhancement signals, respectively. This method has great potential for monitoring MCs in complex systems.
Article
Chemistry, Multidisciplinary
Rongchao Mei, Yunqing Wang, Xizhen Zhao, Shang Shi, Xiaoyan Wang, Na Zhou, Dazhong Shen, Qi Kang, Lingxin Chen
Summary: Skin interstitial fluid (ISF)-based microneedle (MN) sensing using surface-enhanced Raman scattering (SERS) tags allows for the tracking of acute peritonitis progression and evaluation of treatment efficacy.
Article
Environmental Sciences
Zhe Wang, Min Lv, Chu-Long Huang, Dong-Dong Zhang, Ruixia Han, Gang Li, Ling-Xin Chen
Summary: This study found that anthropogenic activities, such as urbanization and oil combustion, have a significant impact on the distribution and properties of dissolved organic matter (DOM) in intertidal sediments. The release of organic matters from urbanization and the combustion of oil contribute to the higher concentration of aromatic compounds in the sediments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Jinglong Han, Huajun Liu, Ji Qi, Jiawen Xiang, Longwen Fu, Xiyan Sun, Liyan Wang, Xiaoyan Wang, Bowei Li, Lingxin Chen
Summary: A novel, simple and effective visual fluorescent sensing paper-based chip was developed for the ultrasensitive detection of mercury ions in environmental water. The method has a low detection limit and fast response time, and successfully achieved trace spiking detection of various water sources.
Article
Chemistry, Analytical
Yan Huang, Lili Fu, Junjun Hou, Lingxiao Wang, Mingzhao Sun, Xiao Sun, Xiaoyan Wang, Lingxin Chen
Summary: Researchers designed and synthesized a nanocomposite RCDs@MnO2 to improve the hypoxic environment of tumors and enhance the efficacy of photodynamic therapy. The nanocomposite could generate 1O2 to achieve the purpose of photodynamic therapy and exhibit fluorescence off-on function in tumor cells and tissues. The results showed that RCDs@MnO2 has the potential to be applied in fluorescence imaging and significantly improve the photodynamic therapy of tumors, both in vitro and in vivo.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Peijun Chai, Xuhui Geng, Ruirui Zhu, Wenpu Wu, Xuesong Wang, Jinhua Li, Longwen Fu, Hongdan Wang, Wanhui Liu, Lingxin Chen, Zhihua Song
Summary: In this study, a novel stationary phase based on carbon dots (CDs), molecularly imprinted polymer (MIP) layer, and silica beads was synthesized to improve the chromatographic separation of polar compounds. The MIP-CDs/SiO2 stationary phase exhibited superior separation efficiency compared to other stationary phases tested, and its chromatographic performance was thoroughly assessed using a variety of compounds. The results showed that the MIP-CDs/SiO2 column combined the desirable properties of MIPs, CDs, and silica microbeads, making it a promising tool in separation science and chromatography-based techniques.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Yixuan Wu, Qian Yu, Younju Joung, Chang Su Jeon, Seunghyun Lee, Sung Hyun Pyun, Sang-Woo Joo, Lingxin Chen, Jaebum Choo
Summary: This study reports the development of a reproducible and highly sensitive surface-enhanced Raman scattering (SERS) substrate using a butanol-induced self-assembly of gold nanoparticles (AuNPs) and its application as a rapid diagnostic platform for SARS-CoV-2. The substrate showed strong electromagnetic enhancement of the Raman signals, enabling the detection of SARS-CoV-2 with a very low limit of detection (LoD) of 3.1 x 10(-15) M. This method provides sufficient sensitivity for SARS-CoV-2 screening assay and reduces the diagnostic time significantly. The results suggest the potential of this approach for developing rapid diagnostic platforms for other biomolecules and infectious diseases.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ji Qi, Bowei Li, Dong Lin, Zhiqiang Du, Longwen Fu, Xiaoyan Wang, Zhiyang Zhang, Liqiang Luo, Lingxin Chen
Summary: To overcome the distortion of fluorescent images by mobile devices, a dual-mode strategy was developed to achieve undistorted visual fluorescent sensing on & mu;PAD by manipulating the coffee-ring effect of the fluid sample. By dividing the resulting fluorescence image into 600 pixels and using a fluorescent probe, accurate quantitative information was obtained to avoid image distortion. This strategy, combined with a small imaging box and a smartphone, allows for rapid testing of histidine in human urine and improved antidistortion for visual fluorescent sensing.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Liyan Bi, Huangruici Zhang, Wenchao Hu, Jiadong Chen, Yixuan Wu, Hao Chen, Bingqian Li, Zhiyang Zhang, Jaebum Choo, Lingxin Chen
Summary: In this study, a novel M13 phage-based SERS nanocarrier was developed for the detection and inactivation of E. coli. The M13 phage was used as a biological scaffold to conjugate with Au@Ag core-shell nanorods (Au@AgNR) and specifically target E. coli. The proposed SERS probe showed a detection limit of 0.5 cfu/mL and high antibacterial efficiency of approximately 90% against E. coli.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Bowei Li, Ji Qi, Feng Liu, Rongfang Zhao, Maryam Arabi, Abbas Ostovan, Jinming Song, Xiaoyan Wang, Zhiyang Zhang, Lingxin Chen
Summary: This article introduces an indirect fluorescence sensing mechanism using the charge transfer effect and smart molecular imprinting technology for the detection of non-fluorescent substances. By immobilizing a molecular imprinted thin film on the surface of nanoparticles and implementing the mechanism on a paper-based microfluidic chip, trace detection of microcystin is achieved.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Environmental
Liyan Wang, Jialuo Yu, Xiaoyan Wang, Jinhua Li, Lingxin Chen
Summary: Environment pollution is a global issue that requires high-performance adsorbent materials for effective removal and remediation. Nanocomposites have emerged as a powerful tool for pollutant removal due to their superior adsorption performances. Among them, molecular imprinting-based nanocomposites with tailor-made binding sites have drawn great research interests in the field of environmental pollutants removal. This article provides a summary of the general fabrication and typical components of molecular imprinting-based nanocomposite adsorbents and discusses synthesis strategies for enhancing the adsorption performances, serving as a background for future research.
JOURNAL OF HAZARDOUS MATERIALS LETTERS
(2023)
Article
Environmental Sciences
Suyu Ren, Xiaojie Jin, Tadiyose Girma Bekele, Min Lv, Jing Ding, Feng Tan, Lingxin Chen
Summary: This study developed a reliable sampling approach, using DGT with XAD-2 as the binding agent, for monitoring 4-MBC in coastal waters. The diffusion coefficients of 4-MBC in freshwater and artificial seawater were determined and the XAD-2 binding gel showed rapid adsorption to 4-MBC. Field deployment showed consistent results with grab sampling, and the DGT method is suitable for seasonal monitoring, source identification, and risk assessment of 4-MBC in coastal waters.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Analytical
Wenpeng Li, Jiawen Xiang, Jinglong Han, Mingsan Man, Lingxin Chen, Bowei Li
Summary: In this study, a rotary microfluidic electrochemical paper-based chip (MIP-ePADs) based on surface biomolecular imprinting technology was proposed for the sensitive and selective detection of IL-6 and PCT. The chip showed stable and accurate analysis and detection capabilities, with low cost. This method is important for the improvement of rapid detection technology, particularly for the analysis of non-antibody inflammation markers.
Article
Engineering, Environmental
Jing Ding, Zhaoqin Liang, Min Lv, Xiuyu Li, Shuang Lu, Suyu Ren, Xiaoyong Yang, Xiaoqiang Li, Chen Tu, Dong Zhu, Lingxin Chen
Summary: This study investigates the effects of soil aging on the toxicity of microplastics to soil fauna. The results show that soil aging slightly alters the surface properties and morphology of microplastics, and significantly affects the release of additives. Additionally, a less diverse microbial community is formed on the surface of microplastics compared to the surrounding soils. Aged microplastics have a significant impact on the reproduction of soil worms, particularly those aged in soil with manure, and disrupt their gut microbiota. Changes in biofilm features and leaching properties of microplastics during aging are the main contributing factors to these shifts. This study provides preliminary insights into the role of soil aging in the toxicity of microplastics to soil fauna.
JOURNAL OF HAZARDOUS MATERIALS
(2024)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
