期刊
BIORESOURCE TECHNOLOGY
卷 313, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.123624
关键词
Chlorella sorokiniana; High protein; Protoplast fusion; Aquaculture
资金
- Innovative Research and Experimental Projects for Young Researchers of Tianjin Academy of Agricultural Sciences [2018007]
- Science and Technology Demonstration Project of One, Two, and Three Industries Fusion Development [17ZXYENC00070]
- Tianjin Enterprise Technology Commissioner Project [19JCTPJC57700]
To improve Chlorella's economic viability as a natural bait in aquaculture, protoplast fusion technology was used for two Chlorella mutants, H10 and Z13, selected by UV and chemical mutagenesis. Chlorella sorokiniana pro-toplast was prepared using the enzyme method, and then the optimal enzyme combination of 4% cellulase and 2% driselase was screened out. Z13 and H10 protoplast preparation rates reached 34.72% and 31.11%, respectively. Nine fusions with higher growth rates were selected to assess their biomass, total and soluble proteins contents. Dry cell weight, total protein, and soluble protein of fusion R7 were 0.92 g.L-1, 67.16%, and 0.59 mg.g(-1), respectively. The biomass was 1.59, 1.43 times that of H10 and Z13; total and soluble proteins increased by 8.89%, 10.25% and 50.12%, 74.62% respectively, compared with the original algae. These results have implications for breeding excellent strains, and for large-scale and optimal application of Chlorella in aquaculture.
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