Article
Biotechnology & Applied Microbiology
Shao-Fei Sun, Jing Yang, Da-Wei Wang, Hai-Yan Yang, Shao-Ni Sun, Zheng-Jun Shi
Summary: Sequential alkaline extraction can significantly improve the enzymatic hydrolysis efficiency of cellulose and comprehensively investigate the structural characteristics of hemicellulose, providing valuable information for the efficient utilization of cellulose and hemicellulose in biorefineries.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Chemistry, Multidisciplinary
Haicheng Xie, Le Zhang, Shunli Jing, Jinwei Zhou, Qing Wu, Yaya Yang, Yufei Chen, Chengyu Yang, Guohua Xia, Yuping Shen, Huan Yang
Summary: This study developed a convenient method to efficiently obtain Prosapogenin A from Protogracillin in Dioscorea zingiberensis tubers using enzymatic hydrolysis. The highest yield reached 96.4%, showing promise for industrial applications.
GREEN CHEMISTRY LETTERS AND REVIEWS
(2022)
Article
Green & Sustainable Science & Technology
Fernando Roberto Paz Cedeno, Breno Belon de Siqueira, Eddyn Gabriel Solorzano Chavez, Ismael Ulises Miranda Roldan, Leonardo Moreira Ropelato, Julian Paul Martinez Galan, Fernando Masarin
Summary: In this study, Eucalyptus by-product was treated with different pretreatment techniques to separate cellulose and lignin. The resulting cellulose and lignin can be used to produce products with high added value and commercial potential, such as cello-oligosaccharides, glucose, and sulfonated lignin.
Article
Biochemistry & Molecular Biology
Rongxin Guo, Xusheng Zheng, Yang Wang, Yiwen Yang, Yifang Ma, Dexun Zou, Yanping Liu
Summary: A novel method of immobilizing cellulase on SA-PEG matrix enabled the enzyme to be used repeatedly with increased activity and immobilization rate. The immobilized enzyme exhibited a wider application range in terms of temperature and pH, as well as improved thermal stability. Moreover, the immobilized cellulase showed significantly higher yields in the enzymatic hydrolysis of MCC compared to free enzyme.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Energy & Fuels
Martua Yan Steward Nababan, Widya Fatriasari, Nyoman J. Wistara
Summary: This study determined the optimum conditions for enzymatic hydrolysis of jabon wood pulp and compared ethanol production using separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes. The results showed that optimized conditions led to a significant increase in ethanol yield and the addition of a surfactant improved cellulose conversion to bioethanol.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Energy & Fuels
Winfred-Peck Dorleku, Richard Bayitse, Anders Cai Holm Hansen, Firibu Kwasi Saalia, Anne-Belinda Bjerre
Summary: This study demonstrates that glucose can be efficiently produced from cassava peel without chemical or hydrothermal pretreatment. The conversion efficiency was optimized using mixed enzymes and response surface methodology. The empirical models developed provide an efficient tool for glucose recovery and have significance for large-scale industrial production.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemistry & Molecular Biology
Rui-Wen Ren, Xiao-Quan Chen, Wen-Hao Shen
Summary: This study demonstrates the preparation of pure spherical CNCs through enzymatic hydrolysis and determines the optimal separation conditions. Compared to MCC, spherical CNCs exhibit lower crystallinity and superior performance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Energy & Fuels
Oznur Yildirim, Dogukan Tunay, Bestami Ozkaya
Summary: This study investigated various pretreatment methods to enhance the biofuel potential of lignocellulosic biomass, and found that acid pretreatment had the highest effect in obtaining reducing sugar. Using the response surface methodology, the optimal conditions for enzymatic hydrolysis were determined as 40 FPU/g biomass, 65-hour retention time, and 45 degrees C temperature. The highest total reducing sugar yield was 30 g fermentable sugar/L or 0.36 g sugar/g biomass, with a bioethanol concentration of 9.5 g/L.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Green & Sustainable Science & Technology
E. Costa, M. F. Almeida, C. Alvim-Ferraz, J. M. Dias
Summary: Crambe abyssinica oil can be used as an alternative feedstock for biodiesel production, and high FAME yield can be obtained through enzymatic transesterification. The methanol:oil molar ratio and enzyme concentration significantly influence the reaction.
Article
Biochemistry & Molecular Biology
Ioanna Stamogiannou, John Van Camp, Guy Smagghe, Davy Van de Walle, Koen Dewettinck, Katleen Raes
Summary: Different classes of phenolic compounds have distinct effects on the enzymatic hydrolysis of cellulose, acting as activators or inhibitors. The presence of the -OCH3 group is found to be crucial for the activating effect of phenolic compounds. Adsorption analysis and SEM images indicate that the activating or inhibiting action of phenolic compounds can lead to different adsorption modes, either on the enzyme or on the substrate.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Green & Sustainable Science & Technology
Aiping Lu, Xiaojie Yu, Li Chen, Clinton Emeka Okonkwo, Phyllis Otu, Cunshan Zhou, Qiaomin Lu, Qiaolan Sun
Summary: This study evaluated the effectiveness of a novel deep eutectic solvent (DES) composed of guaiacol (GG) and p-hydroxybenzoic acid (PB) as hydrogen bond donors, and choline chloride (ChCl) as a hydrogen bond receptor, in the structural properties and enzymatic hydrolysis of peanut shell. The ChCl-GG-PB pretreatment effectively removed hemicellulose and lignin, resulting in a significant improvement in enzymatic glycosaccharification efficiency. Furthermore, the ChCl-GG-PB pretreatment induced significant changes in structure and morphology, with the highest crystal-linity and lowest solid recovery.
Article
Biochemistry & Molecular Biology
Thalita J. J. Bondancia, Camila Florencio, Graziela S. S. Baccarin, Cristiane S. S. Farinas
Summary: Cellulose nanostructures obtained from lignocellulosic biomass by the enzymatic route can offer advantages in terms of material properties and processing sustainability. The presence of auxiliary enzymes can promote the production of nanocellulose with improved properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Agricultural Engineering
David Alexander Mitchell, Isabelle Moreira, Nadia Krieger
Summary: Stochastic models have the potential to be important tools in describing the complex network of reactions involved in enzymatic hydrolysis of polysaccharides, but many current models lack biologically meaningful parameters. Only a few models utilize classical enzyme kinetics parameters, and recent research on stochastic models for cellulose hydrolysis provides valuable insights into the molecular-level phenomenon limiting hydrolysis rates. Further improvements are necessary for stochastic models of enzymatic polysaccharide hydrolysis to become widely used tools for guiding process development.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Physical
Gabriel M. Baggio, Paulo H. Camani, Derval S. Rosa
Summary: This study evaluated the effect of cationic surfactants on the production, surface modification, and properties of cellulose nanostructures. The results showed that different surfactants and concentrations led to changes in the structure and properties of modified CNSs. CTAB exhibited a better shielding effect, reducing the negative charges in nanocellulose. Different surfactants and concentrations also influenced particle size and sugar production.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Materials Science, Paper & Wood
Naveen Kumar, Mahak Mittal, Anita Yadav, Dinesh Kumar Saini, Neeraj K. Aggarwal
Summary: This study found that alkali pretreatment is the best method for increasing the production of reducing sugar from lignocellulosic weed P. hysterophorus. By optimizing the enzymatic hydrolysis process, a higher yield of reducing sugar can be achieved. The results suggest that P. hysterophorus can be a promising and low-cost biomass source for commercial ethanol production.
JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY
(2023)
Article
Energy & Fuels
N. Ibarra-Diaz, J. F. Castanon-Rodriguez, J. Gomez-Rodriguez, M. G. Aguilar-Uscanga
Summary: In this study, the pretreatment and enzymatic hydrolysis of Mexican barley straw were optimized to maximize lignin removal and improve enzyme conversion. The results showed that peroxide-alkaline pretreatment and state-of-the-art enzyme saccharification make barley straw an excellent alternative for fermentable sugar production in various biotech industries.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemistry & Molecular Biology
Maria Ines Infanzon-Rodriguez, Juan Arturo Ragazzo-Sanchez, Sandra del Moral, Montserrat Calderon-Santoyo, Maria Guadalupe Aguilar-Uscanga
Summary: The study evaluated the biochemical characteristics of enzymatic extract from autochthonous fungus Aspergillus niger ITV02 and its application in the enzymatic hydrolysis of wheat straw and corn stubble, showing its potential as an enzyme producer for lignocellulosic hydrolysis.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Polymer Science
M. A. Olivares-Ramirez, L. Lopez-Zamora, M. G. Pena-Juarez, E. J. Gutierrez-Castaneda, J. A. Gonzalez-Calderon
Summary: The present study utilized response surface methodology and central composite design to optimize conditions for inhibiting Staphylococcus aureus growth using TiO2 nanoparticles doped with Ag. The results showed that maximizing inhibition requires intermediate pH levels, high TiO2 solid concentrations, and high AgNO3 levels. The quadratic model obtained displayed a strong adjustment of experimental data with an R-2 value of 0.82.
Article
Agronomy
L. E. Diaz-Nava, M. G. Aguilar-Uscanga, B. Ortiz-Muniz, N. Montes-Garcia, J. M. Dominguez, J. Gomez-Rodriguez
Summary: The study demonstrated that the indigenous yeast Pichia kudriavzevii ITV-S42 with thermotolerance, high resistance, and high productivity showed promising performance in ethanol production, indicating its potential application in both first and second generation ethanol processes.
Article
Energy & Fuels
C. X. Dominguez-Gomez, L. E. Nochebuena-Morando, M. G. Aguilar-Uscanga, L. Lopez-Zamora
Summary: Lignocellulosic biomass is a promising raw material for bioethanol production, but its complex structure hinders the enzymatic saccharification process. Dilute acid and alkaline H2O2 pretreatments are effective methods to reduce costs and enable industrial scaling. The dilute acid pretreatment removed 83.2% of hemicellulose, while the alkaline H2O2 pretreatment removed 56.8% of lignin, and the addition of Tween 80 increased glucose yield.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Energy & Fuels
Alba Jessica Godoy-Salinas, Benigno Ortiz-Muniz, Javier Gomez Rodriguez, Beatriz Gutierrez-Rivera, Maria Guadalupe Aguilar-Uscanga
Summary: The use of raw sugarcane bagasse for cell immobilization by adsorption was evaluated in order to increase ethanol production. The study found that pre-treatment with sulfuric acid was efficient in immobilizing Saccharomyces cerevisiae, and the immobilization process remained stable for up to 20 days. In glucose medium, the packed bed reactor achieved high ethanol productivity, and when using sugarcane juice, ethanol productivity was also satisfactory.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Biotechnology & Applied Microbiology
Daniel A. Zavala-Ortiz, Aurelia Denner, Maria G. Aguilar-Uscanga, Annie Marc, Bruno Ebel, Emmanuel Guedon
Summary: Research indicates that spectroscopic models based on ANNR and SVR show superior performance in cell culture monitoring, with better management of inter-batch heterogeneity and enhanced specificity. PLSR performs less effectively, while SVR and ANNR models show better results for all monitored parameters such as glucose.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Energy & Fuels
A. Varilla-Mazaba, J. A. Raggazo-Sanchez, M. Calderon-Santoyo, S. del Moral, J. Gomez-Rodriguez, M. G. Aguilar-Uscanga
Summary: This study aimed to optimize the acid hydrolysis of sugarcane bagasse to obtain the maximum xylose and minimum acetic acid concentration. Response surface methodology and desirability criterion were used to find the optimal reaction conditions, which were validated through experiments.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Energy & Fuels
Maria Guadalupe Moran-Aguilar, Ivan Costa-Trigo, Alexandra Maria Ramirez-Perez, Esther de Blas, Montserrat Calderon-Santoyo, Maria Guadalupe Aguilar-Uscanga, Jose Manuel Dominguez
Summary: The effectiveness and ease of DES pretreatment in improving biorefinery processes have been demonstrated through studies on sugarcane bagasse and brewery bagasse.
Article
Chemistry, Applied
Y. Perez-Salazar, C. Pena-Montes, S. del Moral, M. G. Aguilar-Uscanga
Summary: This study investigates the activity of cellulases and their production using different raw materials and culture media. The results provide important information for finding low-cost alternatives in cellulase production.
REVISTA MEXICANA DE INGENIERIA QUIMICA
(2022)
Article
Agronomy
Maria Ines Infanzon-Rodriguez, Sandra del Moral, Claudia Castro-Martinez, Cynthia Cano-Sarmiento, Javier Gomez-Rodriguez, Maria Guadalupe Aguilar-Uscanga
Summary: This study utilized multi-objective optimization to maximize cellulase production and improve the enzymatic hydrolysis process of lignocellulosic residues for 2G biofuel production. The results demonstrated the significance of optimizing conditions for enhancing enzyme activity and increasing the yield of fermentable sugars.
Article
Agricultural Engineering
A. Varilla-Mazaba, J. A. Raggazo-Sanchez, M. Calderon-Santoyo, J. Gomez-Rodriguez, M. G. Aguilar-Uscanga
Summary: This study optimized the delignification of sugarcane bagasse using deep eutectic solvents (DES) and found that increasing temperature and reaction time improves lignin removal. The results demonstrated that DES is an efficient and useful method for lignin removal from lignocellulosic biomass, with the added benefit of converting cellulose into fermentable sugars.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Chemistry, Applied
A. Ortega-Platas, V Corro-Herrera, M. G. Aguilar-Uscanga, J. Gomez-Rodriguez
Summary: The study utilized NIRS and chemometrics for real-time monitoring of the xylitol fermentation process, achieving reliable predictions through calibration models and validation criteria, demonstrating the suitability of an in situ NIRS probe for real-time monitoring of xylitol production.
REVISTA MEXICANA DE INGENIERIA QUIMICA
(2021)