期刊
BIOELECTROCHEMISTRY
卷 137, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2020.107684
关键词
Aflatoxin B1; Co-precipitation; Electrophoretic; Immunoelectrode; Mn(2)O(3)nps
资金
- DST [SR/WOS-A/PM-108/2016]
- Department of Science & Technology (DST)
- DST Purse
- JNU
- BDTD Project
- Department of Biotechnology (Indo-Russia Project) Government of India [DBT/IC-2/Indo-Russia/2017-19/02]
This study introduces an electrochemical immunosensor based on manganese oxide nanoparticles for the detection of Aflatoxin-B1. The sensor showed high sensitivity and a low detection limit, making it a promising tool for AFB1 detection in food samples.
This work presents, a manganese oxide nanoparticles (Mn(2)O(3)nps) based electrochemical immunosensor for the detection of Aflatoxin-B1 (AFB1). X-ray diffrraction spectroscopy study confirms the purely synthesized Mn(2)O(3)nps with an average crystallite size of 31.5 nm. Transmission electron microscopy study confirms average particle size of 45 nm. To fabricate an electrochemical biosensor, a thin film of Mn(2)O(3)nps was fabricated onto indium tin oxide (ITO) surface using electrophoretic technique. Such fabricated thin film was utilized to immobilize antibodies (Anti-AFB1) for the selective detection of AFB1 using differential pulse voltammetry technique. Prior to perform sensing, bovine serum albumin (BSA) was utilized to block the uncovered sites on the Anti-AFB1/Mn2O3/ITO immunoelectrode surface. The response of BSA/Anti-AFB1/Mn2O3/ITO immunoelectrode was measured as a function of AFB1 in a linear detection range of 1 pg mL(-1) to 10 mg mL(-1) and sensor showed highest sensitivity of 2.044 lA mL ng(-1)cm(-2) with lower detection limit of 0.54 pg mL(-1). A spiked sample response of corn extract was studied in the linear range of 1 pg mL(-1) to 10 mg mL(-1) and immunoelectrode (BSA/Anti-AFB1/Mn2O3/ITO) showed recovery rate of 98.6 %. (C) 2020 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据