Article
Microbiology
Tianyi Gao, Yingdong Gao, Xiangxiang Liu, Zhenlin Nie, Huilin Sun, Kang Lin, Hongxin Peng, Shukui Wang
Summary: This study analyzed the physicochemical properties, subcellular localization, and biological function of the SARS-COV-2N protein. The identification of 12 phosphorylated sites and 9 potential protein kinase sites in the SARS-COV-2N protein may serve as promising targets for drug discovery and development of a recombinant virus vaccine.
Article
Biology
Leire Egia-Mendikute, Alexandre Bosch, Endika Prieto-Fernandez, So Young Lee, Borja Jimenez-Lasheras, Ana Garcia del Rio, Asier Antonana-Vildosola, Chiara Bruzzone, Maider Bizkarguenaga, Nieves Embade, Ruben Gil-Redondo, Maria Luz Martinez-Chantar, Marcos Lopez-Hoyos, Nicola G. A. Abrescia, Jose M. Mato, Oscar Millet, Asis Palazon
Summary: Egia-Mendikute et al. have developed a multiplexed flow cytometric bead array to detect antibodies reactive to three immunogenic SARS-CoV-2 proteins simultaneously. This assay is more sensitive than ELISA and has superior potential to detect low antibody titers, even in COVID-19-negative individuals.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wajihul Hasan Khan, Nida Khan, Avinash Mishra, Surbhi Gupta, Vikrant Bansode, Deepa Mehta, Rahul Bhambure, M. Ahmad Ansari, Shukla Das, Anurag S. Rathore
Summary: This study demonstrates the significant impact of dimerization of the nucleocapsid protein on sensitivity of COVID-19 diagnostic assays. The dimeric form shows higher recognition by specific monoclonal antibodies and exhibits greater stability and antigenicity compared to the monomeric form. Manufacturers should maintain the monomer-dimer composition for accurate and robust diagnostics.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Medicine, Research & Experimental
Byoung Kwon Park, Jinsoo Kim, Sangkyu Park, Dongbum Kim, Minyoung Kim, Kyeongbin Baek, Joon-Yong Bae, Man-Seong Park, Won-Keun Kim, Younghee Lee, Hyung-Joo Kwon
Summary: This study identified a direct interaction between the S protein and the N protein of MERS-CoV during virus assembly in infected cells, and demonstrated that Spike CD peptides of MERS-CoV inhibit this interaction. Additionally, cell penetration by synthetic Spike CD peptides inhibits viral plaque formation in MERS-CoV-infected cells.
Article
Chemistry, Analytical
Jie Liu, Jinpeng Mao, Mengyu Hou, Zhian Hu, Gongwei Sun, Sichun Zhang
Summary: This article presents a rapid and highly specific method for profiling COVID-19 associated antigen. The method utilizes a nanoparticle scaffold and binding-induced DNA assembly mechanism, with fluorescence translation for detection. The study demonstrates that this method can quantify nucleocapsid protein within 3 minutes, with high recognition ratio and low limit of detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Dai Hatakeyama, Takeshi Masuda, Ryosuke Miki, Sumio Ohtsuki, Takashi Kuzuhara
Summary: Acetylation of viral proteins, such as influenza virus and Zaire Ebolavirus, has been shown to play essential roles in viral replication. Recent research found that the N proteins of SARS-CoV and SARS-CoV-2 were strongly acetylated by human histone acetyltransferases PCAF and GCN5 in vitro, suggesting a crucial role for acetylation in their functions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Yichen Li, Shuaiyao Lu, Jinge Gu, Wencheng Xia, Shengnan Zhang, Shenqing Zhang, Yan Wang, Chong Zhang, Yunpeng Sun, Jian Lei, Cong Liu, Zhaoming Su, Juntao Yang, Xiaozhong Peng, Dan Li
Summary: SARS-CoV-2 infection impairs the disassembly of host stress granules (SGs) and promotes the aggregation of SG-related amyloid proteins, potentially increasing the risk of neurodegeneration.
Article
Biology
J. Rafael Ciges-Tomas, Maria Luisa Franco, Marcal Vilar
Summary: This study presents the crystal structure of the SARS-CoV-2 nucleocapsid protein and its binding with guanine triphosphate. The research reveals a specific interaction between the protein and guanine, which may facilitate the viral assembly process. This finding provides a framework for designing viral assembly inhibitors.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Minghai Chen, Chuang Yan, Fujun Qin, Luping Zheng, Xian-En Zhang
Summary: This study identified 22 protein-protein interactions of SARS-CoV-2 in host cells, with the nucleocapsid (N) protein having the most interactions and specifically interacting with the putative packaging signal (PS) of SARS-CoV-2. The study also demonstrated the functional importance of the PS core for the assembly of viral RNA into virus-like particles (VLPs) and the N-dependent packaging of SARS-CoV-2 RNA.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Airi Rump, Robert Risti, Mai-Ly Kristal, Jekaterina Reut, Vitali Syritski, Aivar Lookene, Sirje Ruutel Boudinot
Summary: The glycosylation of SARS-CoV-2-NP can mask some of its antibody epitopes, potentially leading to false-negative serological test results. Deglycosylation can significantly increase the number of positive tests.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Letter
Virology
Jia He, Jing-Ru Huang, Yao-Lin Zhang, Jiyan Zhang
Summary: The study suggests that SARS-CoV-2 N protein can induce T cell responses, and non-protective T cell responses can be avoided by intranasal vaccination. Experimental results in BALB/c mice demonstrate that intranasal vaccination of SARS-CoV-2 N protein can induce CD8 T cell responses in the lung and CD4 T cell responses in the spleen, while also promoting robust antibody production.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Medical Laboratory Technology
Yu Zhang, Chui Mei Ong, Cassandra Yun, Weike Mo, Jeffery D. Whitman, Kara L. Lynch, Alan H. B. Wu
Summary: Serum nucleocapsid protein of SARS-CoV-2 is a biomarker for acute infection with high diagnostic sensitivity and specificity, which correlates to disease severity and inversely relates to antibody levels.
CLINICAL CHEMISTRY
(2022)
Article
Microbiology
Rebekka F. Thudium, Malene P. Stoico, Estrid Hogdall, Julie Hogh, Henrik B. Krarup, Margit A. H. Larsen, Poul H. Madsen, Susanne D. Nielsen, Sisse R. Ostrowski, Amanda Palombini, Daniel B. Rasmussen, Niels T. Foged
Summary: The newly developed ELISA showed high diagnostic sensitivity and specificity in detecting SARS-CoV-2 nucleocapsid protein, making it a suitable laboratory diagnostic test for COVID-19, particularly in hospitals to facilitate screening of patients, prevention of nosocomial infections, and reduce the requirement for laborious swab sampling and confirmatory PCR analysis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Immunology
Mauricio Menegatti Rigo, Romanos Fasoulis, Anja Conev, Sarah Hall-Swan, Dinler Amaral Antunes, Lydia E. Kavraki
Summary: The COVID-19 pandemic caused by SARS-CoV-2 has led to a global search for new therapies. While most vaccine development has focused on the spike protein, this strategy may contribute to the rise of new variants. Therefore, examining alternative proteins, such as the nucleocapsid (N) protein, is important. The development of the SARS-Arena computational pipeline provides a user-friendly tool for identifying new T-cell targets against SARS-CoV viruses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Su He, Hongwei Gou, Yulin Zhou, Chunxiu Wu, Xinxin Ren, Xiajunpeng Wu, Guanwen Guan, Boxing Jin, Jinhua Huang, Zhigang Jin, Tiejun Zhao
Summary: Viruses use various strategies to suppress the host's innate immune response for viral replication and pathogenesis. The SARS-CoV-2 nucleocapsid protein can inhibit the innate immune response and facilitate viral replication by inducing atypical foci.
Article
Biochemistry & Molecular Biology
Jimcy Platholi, Paul M. Heerdt, H. Y. Lim Tung, Hugh C. Hemmings
JOURNAL OF NEUROCHEMISTRY
(2008)
Article
Endocrinology & Metabolism
Tao T. Zhang, Jimcy Platholi, Paul M. Heerdt, Hugh C. Hemmings, H. Y. L. Tung
METABOLIC BRAIN DISEASE
(2008)
Article
Clinical Neurology
Anne-Marie Bleau, Brian M. Howard, Lauren A. Taylor, Demirkan Gursel, Jeffrey P. Greenfield, H. Y. Lim Tung, Eric C. Holland, John A. Boockvar
NEUROSURGICAL FOCUS
(2008)
Article
Biochemistry & Molecular Biology
S. Andrew Bullock, Jimcy Platholi, Ada Gjyrezi, Paul M. Heerdt, H. Y. Lim Tung, Hugh C. Hemmings
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2007)
Article
Biochemistry & Molecular Biology
A Janoo, PW Morrow, HYL Tung
JOURNAL OF CELLULAR BIOCHEMISTRY
(2005)
Article
Biochemistry & Molecular Biology
PW Morrow, HYL Tung, HC Hemmings
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2004)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)