Article
Microbiology
Sariqa Wagley, Helen Morcrette, Andrea Kovacs-Simon, Zheng R. Yang, Ann Power, Richard K. Tennant, John Love, Neil Murray, Richard W. Titball, Clive S. Butler
Summary: Members of the Proteobacteria can enter a viable but non culturable (VBNC) state as a survival strategy under stressful conditions. Using Vibrio parahaemolyticus as a model organism, distinct subpopulations in the VBNC state with different resuscitation potentials and proteomic profiles were identified. Deletion of the lactate dehydrogenase gene triggered cells to enter VBNC state, while lactate addition extended resuscitation potential. The ability to survive in VBNC state may be linked to overcoming oxidative stress.
Article
Microbiology
Mazen Oneissi, Melissa R. Cruz, Bernardo Ramirez-Zavala, Elena Lindemann-Perez, Joachim Morschhaeuser, Danielle A. Garsin, J. Christian Perez
Summary: This study reveals that neutrophil-derived reactive oxygen species (ROS) direct the subcellular localization and activity of the fungal transcription regulator Rtg1/3, which in turn affects host colonization and pathogenicity of the human fungal pathogen Candida albicans. The oxidants produced by the NOX2 complex serve as a key signal for Rtg1/3's migration to the fungal nucleus, while the MKC1 signaling pathway is implicated in the ROS-dependent regulation of Rtg1/3 in this fungus.
Article
Microbiology
Sandra A. Wilks, Verena V. Koerfer, Jacqui A. Prieto, Mandy Fader, C. William Keevil
Summary: This study compares the development of biofilms on three different types of urinary catheters and finds that biofilms develop rapidly on all materials, with some bacteria remaining viable but not metabolically active. This highlights the need for improved testing before clinical trials are initiated to better assess the effectiveness of antimicrobial catheters.
Article
Biotechnology & Applied Microbiology
Yue Zhang, Xinyu Liao, Jinsong Feng, Donghong Liu, Shiguo Chen, Tian Ding
Summary: Salmonella spp. is a major cause of foodborne outbreaks worldwide. This study found that Salmonella can enter a viable but nonculturable (VBNC) state in liquid egg products, and under certain conditions, can resuscitate. This has important implications for food safety and understanding the health risks associated with Salmonella.
Article
Environmental Sciences
Jiahui Fan, Yangyang Jia, Dongdong Xu, Zhe Ye, Jiahang Zhou, Jionghao Huang, Yulong Fu, Chaofeng Shen
Summary: It was found that Rhodococcus biphenylivorans TG9 enters a viable but nonculturable (VBNC) state under anaerobic conditions, leading to a significant reduction in metabolic activity and degradation ability. However, resuscitation by providing oxygen can restore the degrading capacity of TG9 in the VBNC state, providing a new strategy to cope with survival under anaerobic conditions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Food Science & Technology
Hanxu Pan, Qing Ren
Summary: The viable but nonculturable (VBNC) state is a survival strategy for bacteria in unfavorable conditions. Resuscitation is the recovery of VBNC cells, which is necessary for proof of their existence. The resuscitation of VBNC cells has been widely studied for risk control and potential applications in the food industry.
Review
Microbiology
Peilin Ruan, Mingshu Wang, Anchun Cheng, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Bin Tian, Juan Huang, Xumin Ou, Qun Gao, Di Sun, Yu He, Zhen Wu, Dekang Zhu, Renyong Jia, Shun Chen, Mafeng Liu
Summary: Herpesviruses have evolved various abilities for host infection, including immune escape and induction of cell death. The UL24 protein plays a crucial role in viral infection by inhibiting the host's immune response and contributing to viral proliferation and pathogenicity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Huiying Wang, Yuhong Zhang, Lixia Dai, Xiaoyu Bo, Xiangyun Liu, Xin Zhao, Jie Yu, Lai-Yu Kwok, Qiuhua Bao
Summary: This study investigated the metabolomic differences between VBNC and recovered L. paracasei Zhang cells, revealing the altered physiology of VBNC cells. The results showed that the liquid MRS medium was the most effective in reversing the VBNC state in L. paracasei Zhang.
Article
Food Science & Technology
Eleonora Di Salvo, Felice Panebianco, Antonio Panebianco, Graziella Ziino
Summary: This study found that viable but nonculturable forms of Vibrio parahaemolyticus may be present in frozen bivalve molluscs, suggesting that it could be a potential unrecognized source of food contamination and infection. Further data is needed to perform a robust risk assessment.
Review
Food Science & Technology
Rui Gao, Xinyu Liao, Xihong Zhao, Donghong Liu, Tian Ding
Summary: Viable but nonculturable (VBNC) microorganisms are recognized as pathogenic contaminants in foods and environments, with distinct characteristics that have been used as the basis for developing diagnostic tools. Various methods for examining microorganisms in the VBNC state are currently available, each with its own advantages and drawbacks, and there are also emerging techniques with potential for future applications.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2021)
Article
Biotechnology & Applied Microbiology
Jingbin Zhang, Xiaonan Lu
Summary: Many bacteria, including pathogens like Campylobacter, can enter the viable but nonculturable (VBNC) state to survive in adverse environments. This study explored how food processing conditions and food products could induce Campylobacter jejuni to enter the VBNC state. The results showed that chlorine treatment reduced the culturability but preserved the viability of C. jejuni cells. Under aerobic and low-temperature conditions, approximately 10% of the cells entered the VBNC state. The cells remained viable in ultrahigh-temperature and pasteurized milk, but the number of culturable cells decreased over time.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Victor Jayeola, J. M. Farber, S. Kathariou
Summary: This study found that unique stressors on dried fruit can induce the VBNC state in Salmonella, making it undetectable using culture-based methods. Therefore, additional microscopic and molecular methods should be considered for the accurate detection of all viable and/or culturable cells of Salmonella contaminating dried fruit, as all of these cells have the potential to cause human illness.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yizhe Sun, Dan Lu, Yue Yin, Jia Song, Yang Liu, Wenyan Hao, Fang Qi, Guangze Zhang, Xin Zhang, Liang Liu, Zhiqiang Lin, Hui Liang, Xuyang Zhao, Yan Jin, Yuxin Yin
Summary: PTEN is frequently mutated in human cancers and PTEN alpha, an isoform of PTEN, remains active in cancer bearing stop-gained PTEN mutations. Through counteraction of CD8(+) T cell-mediated cytotoxicity, PTEN alpha promotes T cell dysfunction and accelerates immune-resistant cancer progression. This suggests that PTEN alpha may be a potential target in antitumor immunotherapy.
NATURE COMMUNICATIONS
(2021)
Article
Food Science & Technology
Haiyang Yan, Meng Li, Lingling Meng, Feng Zhao
Summary: The viable but nonculturable (VBNC) state of Staphylococcus aureus can be induced in citric acid buffer at -20 degrees C, causing changes in some biological characteristics, but can be successfully resuscitated under various conditions. The food industry should pay attention to the potential hazard posed by VBNC S. aureus under frozen conditions.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Microbiology
Belen Alvarez, Maria M. Lopez, Elena G. Biosca
Summary: This study investigates the adaptation and resuscitation abilities of Ralstonia solanacearum strains under different temperature and nutrient conditions. The strains show resilience and maintain virulence regardless of their origin in temperate or tropical areas. These findings are important for predicting the spread and pathogenicity of this pathogen in different geographical and climatic regions.