Article
Chemistry, Analytical
F. Vela-Soria, J. Garcia-Villanova, V Mustieles, T. de Haro, J. P. Antignac, M. F. Fernandez
Summary: The study developed a method to determine 10 PFAS in placental tissue, demonstrating its suitability in evaluating fetal exposure to PFAS and providing key data on the subject.
Article
Chemistry, Analytical
Valfredo Azevedo Lemos, Jeferson Alves Barreto, Luana Bastos Santos, Rosivan dos Santos de Assis, Cleber Galvao Novaes, Ricardo J. Cassella
Summary: Dispersive liquid-liquid microextraction (DLLME) is widely used for the separation and preconcentration of various chemical species, with some systems using a syringe as an extraction environment. This review discusses methods utilizing this approach, critical aspects like dispersion promotion, analytical characteristics, and the pros and cons of the methods, as well as future trends in in-syringe microextraction systems.
Article
Biochemical Research Methods
Karen Sze Jie Tay, Michael C. Breadmore, Ee Shan Soh, Hong Heng See
Summary: A new DICM approach coupled with UPLC-MS/MS was demonstrated for the determination of n-nitrosamine impurities in medicinal products, showing good linearity, acceptable reproducibility, low limits of detection, and satisfactory relative recoveries. The method was found to be at least 10 times more sensitive compared to validated direct sample dissolutions coupled with UPLC-MS/MS approach.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Xiaolin Luo, Xiaoyuan Wang, Ming Du, Xianbing Xu
Summary: The determination of flavor compounds in food systems, especially solid samples, can be interfered with by complex food matrices. In this study, dispersive liquid-liquid microextraction (DLLME) was applied prior to headspace solid-phase microextraction (HS-SPME) to reduce the matrix effect in solid seafood samples. The developed method showed high sensitivity, excellent linear relationship, and good recovery rate, and was able to detect maltol and ethyl maltol in selected seafood samples. DLLME coupled with HS-SPME effectively removed the influence of sample matrix and improved the sensitivity of the method.
Article
Biochemistry & Molecular Biology
Chiara Dal Bosco, Francesca Mariani, Alessandra Gentili
Summary: A green solvent-based DLLME/HPLC-MS method has been developed for the determination of 19 pesticides in wine samples. The method shows high recovery, good precision, and low detection limits, making it a quick and cost-effective analysis.
Article
Chemistry, Applied
Ilona Kiszkiel-Taudul, Barbara Starczewska, Milena Jarosz
Summary: Liquid-liquid microextraction procedures using ionic liquid and deep eutectic solvent as the new extractants were developed for the isolation of ampicillin from milk samples. The extraction parameters were optimized and the extracts were analyzed using HPLC-UV and LC-MS/MS techniques. This method can be applied for the determination of ampicillin in various milk samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Environmental Sciences
Magdalena Surma, Piotr Hliwa, Katarzyna Sznajder-Katarzynska, Wieslaw Wiczkowski, Joanna Topolska, Henryk Zielinski
Summary: The study analyzed the presence of PFAS in tissues of pike and roach from two lakes in the Masurian Lake District in Poland, finding four PFAS substances above quantification limits and higher concentrations in kidneys. The findings provide insights into PFAS distribution and accumulation in biota.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Public, Environmental & Occupational Health
Laura Serrano, Luz Ma Iribarne-Duran, Beatriz Suarez, Francisco Artacho-Cordon, Fernando Vela-Soria, Manuela Pena-Caballero, Jose A. Hurtado, Nicolas Olea, Mariana F. Fernandez, Carmen Freire
Summary: A study conducted on breast milk samples from donors to a human milk bank in Spain revealed the presence of various PFAS, including short-chain compounds, with higher concentrations of short-chain PFAS than long-chain ones. Factors such as intake of meat products and use of personal care products like skincare and makeup were found to be associated with increased PFAS concentrations.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2021)
Article
Biochemistry & Molecular Biology
Chiara Dal Bosco, Flavia Bonoli, Alessandra Gentili, Chiara Fanali, Giovanni D'Orazio
Summary: A novel chromatographic application using the nano-LC technique for chiral separation was reported in this study. Chiral recognition of 12 antifungal drugs was achieved through optimized mobile phase composition and experimental conditions. The method demonstrated efficient separation of multiple enantiomers and was successfully applied to the analysis of milk samples.
Article
Chemistry, Analytical
Asmaa Kamal El-Deen, Heba Elmansi, Kuniyoshi Shimizu
Summary: A green vortex-assisted dispersive liquid-liquid microextraction (VA-DLLME) method based on a hydrophobic natural deep eutectic solvent (NaDES) was developed and applied for the preconcentration of anti-prostate cancer triple therapy (flutamide, resveratrol, and ethynylestradiol) from river water and spiked human plasma for the first time. The optimal extraction conditions were determined through a systematic methodology and the method exhibited excellent linearity and precision.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Applied
J. A. Custodio-Mendoza, C. Caamano-Fernandez, M. A. Lage, P. J. Almeida, R. A. Lorenzo, A. M. Carro
Summary: A new, fast, simple, and effective UA-DLLME method was successfully developed and validated for the determination of malondialdehyde, acrolein, and 4-hydroxy-2-nonenal in beverages. The method demonstrated good linearities, recoveries, and reproducibility, and was applied to analyze 60 different beverage samples, showing the presence of secondary lipid oxidation products.
Article
Pediatrics
Arianna Aceti, Andrea Barbarossa, Teresa Gazzotti, Elisa Zironi, Giampiero Pagliuca, Francesca Vitali, Isadora Beghetti, Luigi Corvaglia
Summary: The exposure of preterm infants to PFASs through HM feeding may exceed reference values for older and healthier infants, requiring further investigation into the risks and ways to reduce exposure through maternal exposure and HM feeding.
EUROPEAN JOURNAL OF PEDIATRICS
(2021)
Article
Food Science & Technology
Noelia Pallares, Albert Sebastia, Vicente Martinez-Lucas, Rui Queiros, Francisco J. Barba, Houda Berrada, Emilia Ferrer
Summary: The study found that high-pressure processing was more effective in reducing enniatins in various juice/milk models compared to traditional thermal treatment. Enniatin A1 was the only component successfully reduced in all juice models under the experimental conditions.
Article
Chemistry, Multidisciplinary
Qian Liu, Zhiqiang Li, Liulin Wei, Xiaomei Chen, Yidong Xu, Jingchan Zhao
Summary: In this study, a thermo-switchable deep eutectic solvent was used to improve the extraction of organophosphorus pesticides and pyrethroids in water samples. The method showed fast extraction, high precision, and low detection limit.
ENVIRONMENTAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Applied
Mahboube Shirani, Muhammad Afzal Kamboh, Behrouz Akbari-adergani, Ali Akbari, Sadaf Sadia Arain, Hamid Rashidi Nodeh
Summary: The novel MPSP-nanocomposite was synthesized and applied for SBSDME to detect melamine in milk and milk-based food products. Characterization was done using TEM, SEM-EDX, FT-IR, VSM techniques, and optimal conditions were determined for effective extraction of melamine with a wide linear range and low LOD and LOQ.
Review
Environmental Sciences
L. M. Iribarne-Duran, F. M. Peinado, C. Freire, I Castillero-Rosales, F. Artacho-Cordon, N. Olea
Summary: This systematic review highlights the widespread presence and concentrations of bisphenols, parabens, and benzo-phenones in human breast milk. Variations in levels of these chemicals are observed across geographical regions and time periods, with certain socio-demographic and lifestyle factors also influencing their concentrations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Vicente Mustieles, Andrea Rodriguez-Carrillo, Fernando Vela-Soria, Shereen Cynthia D'Cruz, Arthur David, Fatima Smagulova, Antonio Mundo-Lopez, Alicia Olivas-Martinez, Iris Reina-Perez, Nicolas Olea, Carmen Freire, Juan P. Arrebola, Mariana F. Fernandez
Summary: The study found that childhood BPA exposure was positively associated with thought problems and somatic complaints in adolescence, and there was a correlation between BDNF gene DNA methylation levels and behavioral issues. This supports the conclusions from toxicological studies on the relationship between BPA and behavior, suggesting further research in larger birth cohorts.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Public, Environmental & Occupational Health
Carmen Freire, Luz Maria Iribarne-Duran, Fernando Gil, Pablo Olmedo, Laura Serrano-Lopez, Manuela Pena-Caballero, Jose-Antonio Hurtado, Nelva E. Alvarado-Gonzalez, Mariana F. Fernandez, Francisco M. Peinado, Francisco Artacho-Cordon, Nicolas Olea
Summary: This study measured the concentrations of lead, mercury, cadmium, and arsenic in pooled breast milk samples from a Human Milk Bank in Spain and identified factors associated with these concentrations. The results showed relatively high concentrations of arsenic, moderate concentrations of mercury, and low concentrations of lead and cadmium in the breast milk samples. Factors such as post-partum time, parity, smoking habit, and dietary intake were found to be associated with the metal content of the milk samples.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2022)
Article
Environmental Sciences
Iris Reina-Perez, Alicia Olivas-Martinez, Vicente Mustieles, Elena Salamanca-Fernandez, Jose Manuel Molina-Molina, Nicolas Olea, Mariana F. Fernandez
Summary: This study investigated the combined effect of bisphenol A (BPA) and its substitutes, bisphenol F (BPF) and S (BPS), on the adipogenic differentiation of human adipose-derived stem cells (hASCs). The researchers found that the mixture of bisphenols promoted lipid accumulation in the cells in a dose-dependent manner, with a maximal response at 10 μM. The effect was mediated by the estrogen receptor, and the mixture also altered the expression of adipogenic markers in a non-monotonic dose-response pattern.
Article
Environmental Sciences
Francesca Castiello, Beatriz Suarez, Andrea Beneito, Maria-Jose Lopez-Espinosa, Loreto Santa-Marina, Aitana Lertxundi, Adonina Tardon, Isolina Riano-Galan, Maribel Casas, Martine Vrijheid, Nicolas Olea, Mariana F. Fernandez, Carmen Freire
Summary: This study examined the associations between urinary metabolites of non-persistent pesticides and pubertal development in children from urban and rural areas in Spain. The results showed that higher concentrations of certain pesticide metabolites were associated with increased odds of pubertal development, especially in girls, and BMI may modify these associations.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Carmen Freire, Luz Maria Iribarne-Duran, Fernando Gil, Pablo Olmedo, Laura Serrano-Lopez, Manuela Pena-Caballero, Jose-Antonio Hurtado-Suazo, Nelva E. Alvarado-Gonzalez, Mariana F. Fernandez, Francisco M. Peinado, Francisco Artacho-Cordon, Nicolas Olea
Summary: This study analyzed the concentrations of aluminum, antimony, and lithium in breast milk samples collected from 83 donors in Spain and explored the predictors of these concentrations. The results showed that aluminum was detected in 94% of samples, while antimony and lithium were detected in 72% and 79% of samples, respectively. The concentrations of these elements were mainly associated with dietary habits, lipid content of the samples, and the use of personal care products.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Iris Reina-Perez, Francisco Artacho-Cordon, Vicente Mustieles, Daniel Castellano-Castillo, Fernando Cardona, Inmaculada Jimenez-Diaz, Jose A. Lopez-Medina, Juan Alcaide, Luis Ocana-Wilhelmi, Luz M. Iribarne-Duran, Juan P. Arrebola, Nicolas Olea, Francisco J. Tinahones, Mariana F. Fernandez
Summary: This study suggests that the accumulation of persistent organic pollutants (POPs) in visceral adipose tissue is associated with metabolic syndrome. The levels of certain organic pollutants in adipose tissue were found to be related to indicators of metabolic syndrome such as high blood sugar, high blood pressure, and waist circumference. The study also found that the mixture effect of various organic pollutants was associated with the occurrence of metabolic syndrome.
ENVIRONMENTAL RESEARCH
(2023)
Article
Neurosciences
Alicia Olivas-Martinez, Beatriz Suarez, Elena Salamanca-Fernandez, Iris Reina-Perez, Andrea Rodriguez-Carrillo, Vicente Mustieles, Nicolas Olea, Carmen Freire, Mariana F. Fernandez
Summary: This study aimed to develop, standardize, and validate a methodology to measure BDNF protein levels in urine samples for biomonitoring studies. After testing different experimental conditions and ELISA kits, it was found that the best results were obtained with 0.6 ml of acidified and extracted urine samples, using the Raybiotech ELISA kit. The developed methodology showed good sensitivity and reproducibility, suggesting its potential usefulness in diagnosing and monitoring neurological impairments.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Environmental Sciences
Francesca Castiello, Beatriz Suarez, Jose Gomez-Vida, Maties Torrent, Mariana F. Fernandez, Nicolas Olea, Carmen Freire
Summary: This study found that certain pesticides, such as DETP, TCPy, and MDA, when present in urinary biomarkers above a certain threshold, were associated with delayed sexual maturity in adolescent males. Conversely, detectable concentrations of 1-NPL were associated with adrenal development but negatively correlated with testicular volume maturity.
Article
Public, Environmental & Occupational Health
Andrea Rodriguez-Carrillo, Vicente Mustieles, Elena Salamanca-Fernandez, Alicia Olivas-Martinez, Beatriz Suarez, Lola Bajard, Kirsten Baken, Ludek Blaha, Eva Cecilie Bonefeld-Jorgensen, Stephan Couderq, Shereen Cynthia D'Cruz, Jean-Baptiste Fini, Eva Govarts, Claudia Gundackerj, Antonio F. Hernandez, Marina Lacasana, Federica Laguzzi, Birgitte Linderman, Manhai Long, Henriqueta Louro, Christiana Neophytou, Axel Oberemn, Sylvie Remy, Anna Kjerstine Rosenmai, Anne Thoustrup Saberr, Greet Schoeters, Maria Joao Silvan, Fatima Smagulova, Maria Uhl, Anne Marie Vinggaard, Ulla Vogel, Maria Wielsoe, Nicolas Oleaa, Mariana F. Fernandeza
Summary: Human biomonitoring studies have found widespread daily exposure to environmental chemicals, some of which are suspected to contribute to adverse health outcomes. The HBM4EU initiative aims to develop informative effect biomarkers for large-scale European HBM studies, providing mechanistic information on early and late adverse effects. A stepwise strategy has been developed to identify and implement validated effect biomarkers, and their ability to detect early biological effects and identify high-risk subgroups has been demonstrated.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2023)
Article
Environmental Sciences
Andrea Rodriguez-Carrillo, Sylvie Remy, Shereen Cynthia D'Cruz, Elena Salamanca-Fernandez, Fernando Gil, Pablo Olmedo, Vicente Mustieles, Fernando Vela-Soria, Kirsten Baken, Nicolas Olea, Fatima Smagulova, Mariana F. Fernandez, Carmen Freire
Summary: This study found that kisspeptin serum protein and DNA methylation levels in adolescent males were associated with exposure to environmental chemicals and serum reproductive hormone levels. This suggests that kisspeptin may be a promising effect biomarker.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Andrea Rodriguez-Carrillo, Sylvie Remy, Gudrun Koppen, Natasha Wauters, Carmen Freire, Alicia Olivas-Martinez, Tessa Schillemans, Agneta Akesson, Anteneh Desalegn, Nina Iszatt, Elly den Hond, Veerle Verheyen, Lucia Fabelova, Lubica Palkovicova Murinova, Susana Pedraza-Diaz, Argelia Castana, Jose Vicente Garcia-Lario, Bianca Cox, Eva Govarts, Kirsten Baken, Manuel Tena-Sempere, Nicolas Olea, Greet Schoeters, Mariana F. Fernandez
Summary: Exposure to PFAS can impair human reproductive function, and this study aimed to evaluate its relationship with the HPG axis through analyzing reproductive hormones and kisspeptin levels. PFAS compounds and reproductive markers were measured in 733 European teenagers. Results showed sex-specific associations, with higher testosterone levels in girls and lower FSH levels in boys associated with PFAS mixture. This study enhances our understanding of the adverse effects of PFAS on the HPG axis.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Andrea Rodriguez-Carrillo, Elena Salamanca-Fernandez, Elly den Hond, Veerle J. Verheyen, Lucia Fabelova, Lubica Palkovicova Murinova, Susana Pedraza-Diaz, Argelia Castano, Jose Vicente Garcia-Lario, Sylvie Remy, Eva Govarts, Greet Schoeters, Nicolas Olea, Carmen Freire, Mariana F. Fernandez
Summary: This study investigated the association between exposure to PFAS and phthalates and serum levels of thyroid hormones in European adolescents, and found sex-specific differences in the effects.
ENVIRONMENTAL RESEARCH
(2023)
Article
Public, Environmental & Occupational Health
Carmen Freire, Fernando Vela-Soria, Francesca Castiello, Elena Salamanca-Fernandez, Raquel Quesada-Jimenez, Maria Cristina Lopez-Alados, Mariana F. Fernandez, Nicolas Olea
Summary: This study investigated the association between plasma concentrations of PFAS and serum levels of thyroid hormones in adolescent males. The results showed that exposure to PFAS was associated with an increase in free T4 levels in boys with lower iodine intake. However, the extent of these alterations remains uncertain due to the small sample size.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2023)
Article
Reproductive Biology
Maria Wielsoe, Jose-Manuel Molina-Molina, Andrea Rodriguez-Carrillo, Vicente Mustieles, Nicolas Olea, Mariana F. Fernandez, Eva Cecilie Bonefeld-Jorgensen
Summary: Humans are exposed to complex chemical mixtures that can have combined effects on human health. This study extracted the actual mixture of PFAS from human placenta samples and assessed its xeno-estrogenic activity using two bioassays. Most of the PFAS extracts showed xeno-estrogenic activity in one or both assays. The activities were associated with maternal characteristics but not with fetal growth.
REPRODUCTIVE TOXICOLOGY
(2023)
