期刊
ANALYTICA CHIMICA ACTA
卷 1129, 期 -, 页码 40-48出版社
ELSEVIER
DOI: 10.1016/j.aca.2020.06.040
关键词
MUC1; M-shaped aptamer probe; AgNCs-Hairpin; Cascade signal amplification; Human serum
资金
- National Natural Science Foundation of China [81771905]
- Scientific Research Foundation of Jiangsu Commission of Health [H2018068]
- Traditional Chinese Medicine Technology Project of Wuxi Administration of Traditional Chinese Medicine [ZYKJ201903]
- Innovation Capacity Development Plan of Jiangsu Province [BM2018023]
- Jiangsu Provincial Key Medical Discipline [ZDXKA2016017]
A novel label-free fluorescent biosensing strategy was described for the sensitive detection of mucin 1 (MUC1). It consisted of an M-shaped aptamer probe for exonuclease I (Exo 1)-assisted target recycling (EATR) amplification, and two AgNCs-hairpin probes for graphene oxide (GO)-assisted hybridization chain reaction (HCR) amplification. Based on the specificity of aptamer-target recognition, the addition of MUC1 caused a conformational change in the M-shaped aptamer probe, which was split into a MUC1-P3 complex and a P1-P2 duplex. Exo I then catalyzed the cleavage of aptamer sequence P3 from the MUC1P3 complex and released the target MUCl. The released target MUC1 was free to bind with a new Mshaped probe to perform EATR amplification. Furthermore, the P1-P2 duplex with three single-stranded arms can act as a primer to initiate HCR between hairpin probes AgNCs-H1 and AgNCs-H2. In the process of HCR, two AgNCs-hairpins were autonomously cross-opened, generating long linear double-stranded nanowires containing large numbers of AgNCs. These nanowires cannot be quenched by GO due to the weak affinity between the long double-stranded DNA and GO, thereby retaining a strong fluorescent signal indicative of the concentration of MUCl. With these designs, in addition to an extremely low detection limit of 0.36 fg mL(-1), the method exhibited an acceptable linear response to detect MUC1 from 1 fg mL(-1) to 1 ng mL(-1). Additionally, this method could be exerted with a high degree of success to detect MUC1 in diluted human serum with satisfactory results. (C) 2020 Elsevier B.V. All rights reserved.
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