Article
Genetics & Heredity
Jun He, Yong Zhao, Shuangshuang Zhang, Yanze He, Jiafu Jiang, Sumei Chen, Weimin Fang, Zhiyong Guan, Yuan Liao, Zhenxing Wang, Fadi Chen, Haibin Wang
Summary: This study used oligonucleotide fluorescence in situ hybridization (Oligo-FISH) to draw ideograms of rDNA sites in Chrysanthemum accessions and found polymorphisms and co-localization of rDNA sites. There was also a correlation between the number of 5S rDNA sites and chromosome number, and the geographical distribution of different ploidy Chrysanthemum accessions may have promoted karyotype evolution. The study identified the formation mechanism of rDNA variations and provided a reliable method for examining rDNA variations in Chrysanthemum and related species, contributing to understanding the evolutionary and phylogenetic relationships of the Chrysanthemum genus.
Article
Biochemistry & Molecular Biology
Jun He, Sisi Lin, Zhongyu Yu, Aiping Song, Zhiyong Guan, Weimin Fang, Sumei Chen, Fei Zhang, Jiafu Jiang, Fadi Chen, Haibin Wang
Summary: Using rDNA as a FISH probe, researchers found that 5S rDNA sites are mainly located in subterminal chromosome regions, while the number and intensity of 45S rDNA sites differ from previously studied Chrysanthemum resources. These findings offer insight into the chromosomal structural variations in Chrysanthemum and its related species.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Plant Sciences
Yurij O. Tynkevich, Antonina Y. Shelyfist, Liudmyla Kozub, Vera Hemleben, Irina I. Panchuk, Roman A. Volkov
Summary: This study evaluates the molecular organization and diversity of the intergenic spacer region (IGS) in the Solanum genus. The study finds that the main mechanism of IGS molecular evolution is the step-wise accumulation of single base substitution or short indels. The study also reconstructs the phylogeny of the Solanum genus using IGS sequences but identifies certain species that differ from previous results and require further clarification.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Marlon Garcia Paitan, Maricielo Postillos-Flores, Luis Rojas Vasquez, Maria Siles Vallejos, Alberto Lopez Sotomayor
Summary: In this study, FISH mapping and flow cytometry were used to assess genetic differences between wild and cultivated populations of Physalis peruviana L. (goldenberry). The results showed genetic differences at the molecular cytogenetic level and in genome size between the two populations. These findings have important implications for future cytogenetic and genomic studies as well as breeding programs in goldenberry populations.
Article
Biochemistry & Molecular Biology
Magdalena Senderowicz, Teresa Nowak, Hanna Weiss-Schneeweiss, Laszlo Papp, Bozena Kolano
Summary: This study investigated the chromosome evolution of Crepis plants by analyzing the chromosome base number and organization of rDNA loci in 39 species. The results showed that despite variations in rDNA loci organization, most species had rDNA loci on the same chromosome arm. Comparative analysis revealed multiple independent gains of rDNA loci and repositioning during diversification and speciation. These findings provide important insights into the evolutionary mechanisms and speciation of Crepis plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Anton Stepanenko, Guimin Chen, Phuong T. N. Hoang, Joerg Fuchs, Ingo Schubert, Nikolai Borisjuk
Summary: This study comprehensively characterized the chromosomal locations and molecular organization of 35S and 5S rDNA genes in water lettuce using karyological and molecular methods. Single locations for the 35S and 5S rDNA loci were identified using fluorescence in situ hybridization. The molecular cloning and nucleotide sequencing of 35S rDNA revealed typical structural characteristics and allowed for phylogenetic analysis. The 5S rDNA locus showed variation in nucleotide composition and subrepeat number, confirming the mosaic composition of the gene locus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Horticulture
Guangxin Liu, Yue Lan, Lianwei Qu, Yilian Zhao, Haoyang Xin, Mengli Xi
Summary: Through our research on wild species and cultivars, we have clarified the genetic relationships between different sections of tulips, as well as the differences and variations in the 5S rDNA sequences. Our findings provide clearer and more stable evidence for the classification of tulips.
SCIENTIA HORTICULTURAE
(2022)
Article
Cell Biology
Barbora Augstenova, Eleonora Pensabene, Lukas Kratochvil, Michail Rovatsos
Summary: Anguimorphan lizards are a group of squamate reptiles with wide geographical distribution and morphological variability. Their karyotypes show significant variation across different lineages, with Anguids exhibiting the most variability. The study documented differentiated sex chromosomes in monitors and helodermatids, as well as in some anguids, and also revealed a rare case of spontaneous triploidy in Varanus primordius.
Article
Biochemistry & Molecular Biology
Alexander Kostmann, Barbora Augstenova, Daniel Frynta, Lukas Kratochvil, Michail Rovatsos
Summary: Lizards of the Scincoidea clade, including cordylids, gerrhosaurids, skinks, and xantusiids, exhibit almost worldwide distribution and significant ecological and morphological diversity. Despite limited variability in cytogenetic traits, different species show conservation in diploid chromosome numbers but significant variation in chromosome morphology and the presence of interstitial telomeric sequences. Sex chromosomes, identified as XX/XY, are only found in Tiliqua scincoides within this clade, indicating a generally poor stage of differentiation in sex chromosomes among these lizards.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Jin Chai, Li Xue, Jiawei Lei, Wei Yao, Muqing Zhang, Zuhu Deng, Fan Yu
Summary: This study used chromosome-specific probes and fluorescence in situ hybridization (FISH) to investigate chromosome inheritance during hybridization in sugarcane breeding. The results revealed complete doubling of S. officinarum chromosomes in most hybrids. However, defective chromosome doubling was observed in one hybrid. These findings provide important chromosome markers for further studies on chromosome doubling and chromosomal inheritance in sugarcane breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Fisheries
Hui Zhang, Yu Sun, Shurun Zhu, Wangchao He, Luojing Zhou, Rurong Zhao, Kaikun Luo, Chenchen Tang, Chun Zhang, Shaojun Liu
Summary: The study revealed that strong signals of 5S rDNA from the RCC genome appeared in the pachytene and metaphase stages of meiosis I cells in 4nAT, indicating pairing of homologous chromosomes in 4nAT occurred between identical chromosomes. Additionally, all bivalent pairs possessed one monochiasmate and were neatly arranged in the equatorial plate during meiosis I in 4nAT, ensuring normal separation of homologous chromosomes.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2022)
Article
Limnology
LIU Yu, LIU Pengfei, BI Yanhui, ZHOU Zhigang
Summary: The linkage of 5S rRNA gene to 18S-5.8S-25S rRNA gene was reported in some species in Ochrophyta. Dual-color fluorescence in situ hybridization technique revealed the localization and physical unlinkage of rRNA genes in Saccharina japonica, providing insights into the cytogenetics of this kelp species.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2021)
Article
Genetics & Heredity
Kevin Santos da Silva, Larissa Glugoski, Marcelo Ricardo Vicari, Augusto Cesar Paes de Souza, Renata Coelho Rodrigues Noronha, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
Summary: The Ancistrus genus demonstrates extensive chromosomal diversity, including the occurrence of heteromorphic sex chromosomes and unstable repetitive sequences. These findings provide important insights into the evolution and genetic mechanisms of the Ancistrus genus.
FRONTIERS IN GENETICS
(2022)
Article
Genetics & Heredity
Qingfeng Liu, Xuanyi Zhang, Junmei Liu, Fanglei Liu, Fangming Shi, Qinbo Qin, Min Tao, Chenchen Tang, Shaojun Liu
Summary: This study obtained an allodiploid fish BR with 49 chromosomes through distant hybridization, which showed significantly different morphological traits and growth rates compared to its parent species. The 5S rDNA of BR inherited specific bands from its parents and exhibited obvious recombination and heredity.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Analiza Fernanda Cavenagh, Matheus Pires Rincao, Felipe Cordeiro Dias, Antonio Domingos Brescovit, Ana Lucia Dias
Summary: The study reveals intra and interspecific chromosomal variability in the genus Lycosa, suggesting that chromosomal rearrangements are associated with the diversification of diploid number and sexual chromosome systems in this group of spiders.
GENETICS AND MOLECULAR BIOLOGY
(2022)