Article
Microbiology
Claudia Honsig, Brigitte Selitsch, Marlene Hollenstein, Matthias G. Vossen, Kathrin Spettel, Birgit Willinger
Summary: In this study, the identification of 230 filamentous fungi isolates using MALDI-TOF MS in combination with the Filamentous Fungi Library 3.0 was investigated. The results showed that using the more time-consuming sample preparation methods improved species-level identification rates, and the in-house threshold for species identification was validated for routine use in the laboratory. This suggests that this commercial platform is suitable for routine diagnosis.
Article
Microbiology
Jang-Jih Lu, Hsiu-Jung Lo, Chih-Hua Lee, Mei-Jun Chen, Chih-Chao Lin, Yin-Zhi Chen, Ming-Horng Tsai, Shao-Hung Wang
Summary: This study used MALDI-TOF MS to identify yeast-like microorganisms in the oral washes of non-bedridden nursing home residents. The majority of isolates were Candida species (78.1%). MALDI-TOF MS was verified as a fast, accurate, and practical method for analyzing oral yeasts in elderly subjects.
Article
Mycology
Husam Salah, Anna Kolecka, Anna Rozaliyani, Retno Wahyuningsih, Saad J. Taj-Aldeen, Teun Boekhout, Jos Houbraken
Summary: In this study, the authors developed an improved method for the identification of Aspergillus species using MALDI-TOF MS. By cultivating the fungi on a polycarbonate filter, they were able to obtain reliable mass spectra and achieve higher accuracy in species identification compared to the recommended liquid cultivation method. This method showed promise for the identification of other filamentous fungi as well.
Article
Agronomy
Jadwiga Stanek-Tarkowska, Milosz Pastuszczak, Ewa Szpunar-Krok, Miroslava Kacaniova, Maciej Ireneusz Kluz, Ewa Antonina Czyz, Rafal Pieniazek, Karol Skrobacz, Karol Pietrzyk
Summary: The aim of the research was to investigate the impact of different doses of ash from biomass combustion on the number and community of soil bacteria. The study found that the use of biomass ash as fertilizer led to an increase in the number of microorganisms, particularly at doses ranging from 200 to 500 kg K-2 Oha(-1). Soil moisture, in addition to ash dosage, was identified as an important factor in bacterial growth. The highest amount of soil bacteria was observed in these variants, and a total of 44 bacterial species belonging to 5 genera were identified.
Review
Environmental Sciences
Mohammad Y. Ashfaq, Dana A. Da'na, Mohammad A. Al-Ghouti
Summary: Bacteria play diverse roles in the environment, with MALDI-TOF MS technology gaining attention for routine bacterial identification. This technique has been applied in various environmental studies, showing benefits in rapid and cost-effective bacteria identification.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Biochemistry & Molecular Biology
Michal Zloch, Ewelina Maslak, Wojciech Kupczyk, Marek Jackowski, Pawel Pomastowski, Boguslaw Buszewski
Summary: This study evaluated the use of different culture media in reflecting the microbial compositions of diabetic foot infections in Poland. The results showed significant differences in species coverage and efficiency between the investigated media. Selecting the appropriate media set can significantly improve the accuracy of reflecting the microbial compositions of diabetic foot infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xia Wang, Guanqi Wu, Shuo Han, Jingjing Yang, Xiangwei He, Haifang Li
Summary: Plant endophytic bacteria play important roles in plant growth and stress resistance. This study identified and classified endophytic bacteria in Populus based on MALDI-TOF MS data and 16S rRNA gene sequencing, and verified the classification capacity of protein mass fingerprints. The results provide a valuable reference for differentiating and identifying endophytic bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Emilie Cardot Martin, Claudine Renaux, Emilie Catherinot, Lucie Limousin, Louis Jean Couderc, Marc Vasse
Summary: This study evaluated the new ID-FUNGI plate for mold identification using MALDI Biotyper, and found that IDFP significantly improved species identification compared to traditional Sabouraud agar with gentamicin and chloramphenicol. The results of the study suggest that the new IDFP can enhance mycological diagnostic accuracy and workflow in laboratories.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Monika Borkowska, Ewelina Celinska
Summary: Yeasts play an important role in food production, and reliable methods for yeast identification and differentiation are needed to monitor production processes and ensure the authenticity and safety of foods. In this study, a qPCR-HRM protocol based on three targeted regions was developed for rapid differentiation of yeast species isolated from food products. The simultaneous analysis of the three regions resulted in rapid and accurate differentiation of all the yeast strains at species-level resolution.
Article
Immunology
Qiaolian Yi, Meng Xiao, Xin Fan, Ge Zhang, Yang Yang, Jing-Jia Zhang, Si-Meng Duan, Jing-Wei Cheng, Ying Li, Meng-Lan Zhou, Shu-Ying Yu, Jing-Jing Huang, Xin-Fei Chen, Xin Hou, Fanrong Kong, Timothy Kudinha, Ying-Chun Xu
Summary: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a rapid, accurate, and less labor-intensive method for identifying microorganisms, but its effectiveness in closely related yeast species has been limited. This study evaluated the performance of two commercially available MALDI-TOF systems, Autof MS 1000 and Vitek MS, in identifying yeasts within closely related species complexes. Autof MS 1000 showed good capacity for yeast identification, while Vitek MS had lower accuracy, particularly in less common species within phylogenetically closely related species complexes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Jiaojiao Sun, Haimei Shi, Yuyan Xue, Wenmin Cheng, Menghuan Yu, Chuanfan Ding, Fuxing Xu, Shaoning Yu
Summary: The accuracy of bacterial identification based on MALDI-TOF MS can be affected by functional proteins, releasing bacteria from functional material can increase accuracy.
Article
Chemistry, Analytical
Yuexin Li, Tao Wang, Jianmin Wu
Summary: Fast bacterial identification in urine samples was achieved by capturing bacteria on a microchannel silicon nanowire microfluidic chip, followed by MALDI-TOF MS detection. Bacteria with a concentration of 10(6) CFU mL(-1) in urine samples could be identified without culture under optimized conditions. Furthermore, after 4 hours of culture, bacteria with a concentration as low as 10(3) CFU mL(-1) could be identified.
Article
Microbiology
Adriana Calderaro, Mirko Buttrini, Benedetta Farina, Sara Montecchini, Monica Martinelli, Federica Crocamo, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto
Summary: Colistin resistance is a major threat to global public health, and this study evaluated a MALDI-TOF mass spectrometry peak-based assay to distinguish colistin resistant and susceptible E. coli strains. A classifying algorithm model was developed, with SNN and GA algorithms showing the best performances in recognizing strains.
Article
Immunology
Ya-Ting Ning, Wen-Hang Yang, Wei Zhang, Meng Xiao, Yao Wang, Jing-Jia Zhang, Ge Zhang, Si-Meng Duan, Ai-Ying Dong, Da-Wen Guo, Gui-Ling Zou, Hai-Nan Wen, Yan-Yan Guo, Li-Ping Chen, Miao Chai, Jing-Dong He, Qiong Duan, Li-Xia Zhang, Li Zhang, Ying-Chun Xu
Summary: The study developed two rapid protein extraction methods (ZSB and FUS) for filamentous fungi, significantly reducing the operating time before MALDI-TOF MS analysis and improving identification accuracy. Compared to the routine method, the two novel methods could better identify isolates from most mold types.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Nurhan Ertas Onmaz, Candan Gungor, Serhat Al, Adalet Dishan, Harun Hizlisoy, Yeliz Yildirim, Filiz Kasap Tekinsen, H. Burak Disli, Mukaddes Barel, Fulden Karadal
Summary: This study found potential contamination of toxigenic fungi in Turkish mould-matured cheese, but no mycotoxins were detected in the cheese samples. Selecting non-toxigenic fungi for cheese production and controlling ripening conditions are critical for ensuring the quality and safety of Turkish cheese.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Microbiology
Joanna Bucka-Kolendo, Barbara Sokolowska, Stanislaw Winiarczyk
Review
Chemistry, Physical
Monika Parcheta, Renata Swislocka, Sylwia Orzechowska, Monika Akimowicz, Renata Choinska, Wlodzimierz Lewandowski
Summary: This study focuses on the role of antioxidants in healthy foods, particularly examining the properties of effective antioxidants and the impact of complexed ligands with selected metals on antioxidant effects. Experimental findings suggest that ligand antioxidants complexed with high ion potential metals exhibit enhanced antioxidant properties.
Review
Food Science & Technology
Paulina Srednicka, Edyta Juszczuk-Kubiak, Michal Wojcicki, Monika Akimowicz, Marek L. Roszko
Summary: Food contaminations pose a serious threat to global food safety, and probiotics offer a promising approach to reduce the risk related to the presence of xenobiotics in foodstuffs. Probiotics have been proven to be an effective tool in preventing dysbiosis and alleviating toxicity induced by xenobiotics.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Review
Pharmacology & Pharmacy
Monika Kowalczyk, Aleksandra Golonko, Renata Swislocka, Monika Kalinowska, Monika Parcheta, Artur Swiergiel, Wlodzimierz Lewandowski
Summary: Phenolic compounds show promising antiviral potential by inhibiting virus multiplication at various stages of the viral life cycle and indirectly affecting host cell's biochemical processes used by viruses. Metal complexation of phenolic compounds may enhance their antiviral properties by affecting key structural elements of SARS CoV-2.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Microbiology
Michal Wojcicki, Olga Swider, Kamila J. Daniluk, Paulina Srednicka, Monika Akimowicz, Marek L. Roszko, Barbara Sokolowska, Edyta Juszczuk-Kubiak
Summary: The widespread use of antibiotics has led to the development of multidrug resistance in many bacterial strains, including Salmonella. Salmonella, a common pathogen causing intoxication from contaminated food and water, is pharmacologically treated with antibiotics like fluoroquinolones, ceftriaxone, and azithromycin. Understanding the molecular mechanisms behind antibiotic resistance in Salmonella is essential for choosing effective treatment and addressing the significant global concern of increasing antibiotic resistance.
Article
Biochemistry & Molecular Biology
Michal Wojcicki, Paulina Srednicka, Stanislaw Blazejak, Iwona Gientka, Monika Kowalczyk, Paulina Emanowicz, Olga Swider, Barbara Sokolowska, Edyta Juszczuk-Kubiak
Summary: This study isolated and characterized four bacteriophages with lytic activity against saprophytic bacterial microflora in minimally processed plant-based food products, showing promising potential as a biological control agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Joanna Bucka-Kolendo, Edyta Juszczuk-Kubiak, Barbara Sokolowska
Summary: Lactic acid bacteria in the natural environment encounter multiple stressors, but they are not subjected to high hydrostatic pressure (HHP), which makes their response to HHP more difficult to identify. DnaK, ctsR, and hrcA have been found to be essential regulators in the LAB cellular response to HHP, serving as useful markers for this purpose.
Article
Microbiology
Despoina Eugenia Kiousi, Joanna Bucka-Kolendo, Adrian Wojtczak, Barbara Sokolowska, Agapi I. I. Doulgeraki, Alex Galanis
Summary: This study presents the annotated whole genome sequence of two strains of Loigolactobacillus backii, a beer-spoiling bacteria with high hop tolerance. The genetic basis and adaptation of the strains in the brewery environment were studied, revealing their ability to survive and grow in the presence of hop compounds. The findings provide insights into the mechanisms behind the tolerance of Lg. backii to extreme conditions in the brewery.
Article
Genetics & Heredity
Joanna Bucka-Kolendo, Despoina Eugenia Kiousi, Adrian Wojtczak, Agapi I. Doulgeraki, Alex Galanis, Barbara Sokolowska
Summary: Among beer-spoiling microorganisms, dominant species include Lactobacillus, Leuconostoc, Oenococcus, and Pediococcus. High hydrostatic pressure can eliminate these microorganisms while preserving desired beer properties. This study analyzed the resistance of a beer-spoiling Lactiplantibacillus plantarum strain to hop and high hydrostatic pressure, and confirmed its genome characteristics through sequencing. The findings suggest the strain's growth is affected by beer concentration and it possesses genes for stress resistance and adaptation to the brewing environment.
Article
Food Science & Technology
Joanna Bucka-Kolendo, Barbara Sokolowska
Summary: This study evaluated the impact of high hydrostatic pressure (HHP) on stress-response mechanism-related genes in lactic acid bacteria (LAB). The findings showed changes in gene expression after HHP treatment, but no correlation with single nucleotide polymorphisms (SNPs). The number and diversity of mutations varied among different genes. Further research is needed to understand the general mechanisms and changes in membranes' composition, proteome, and gene expression patterns.
QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS
(2022)
Review
Biochemistry & Molecular Biology
Joanna Bucka-Kolendo, Barbara Sokolowska
ACTA BIOCHIMICA POLONICA
(2017)