Article
Biophysics
Wenyuan Kang, Lin Liu, Peihang Yu, Tianyi Zhang, Chunyang Lei, Zhou Nie
Summary: The study demonstrates the use of a switchable Cas12a for direct analysis of histone deacetylase (HDAC) activity. The inactivation of Cas12a by acetylation can be reversed by HDAC-mediated deacetylation. By leveraging this switchable Cas12a, a sensitive one-pot assay for detecting HDAC activity was developed, with sub-nanomolar sensitivity. This assay can be applied in biochemical studies and clinical diagnostics.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Immunology
Maurizio Bruschi, Andrea Angeletti, Xhuliana Kajana, Gabriella Moroni, Renato Alberto Sinico, Micaela Fredi, Augusto Vaglio, Lorenzo Cavagna, Federico Pratesi, Paola Migliorini, Francesco Locatelli, Giulia Pazzola, Giampaola Pesce, Marcello Bagnasco, Angelo Manfredi, Giuseppe Alvise Ramirez, Pasquale Esposito, Simone Negrini, Federica Bui, Barbara Trezzi, Giacomo Emmi, Ilaria Cavazzana, Valentina Binda, Paride Fenaroli, Isabella Pisani, Carlomaurizio Montecucco, Domenico Santoro, Francesco Scolari, Stefano Volpi, Marta Mosca, Angela Tincani, Giovanni Candiano, Enrico Verrina, Franco Franceschini, Angelo Ravelli, Marco Prunotto, Pier Luigi Meroni, Gian Marco Ghiggeri
Summary: This study found that anti-Annexin A1 IgG2 competitively and dose-dependently binds to dsDNA at physiological acid-base balance, and the competition is null at pH 9. It also showed that the binding of both dsDNA and Annexin A1 to their respective antibodies is strictly pH-dependent, with minimal binding at pH 4 and maximal binding at pH 9-10. The anionic charge of dsDNA is mainly conferred by phosphatidic residues. These findings suggest that dsDNA may interact with antibodies raised versus other anionic molecules due to charge mimicry, contributing to the generation of anti-dsDNA antibodies.
JOURNAL OF AUTOIMMUNITY
(2022)
Article
Biochemistry & Molecular Biology
Malte Lenders, David Scharnetzki, Ali Heidari, Daniele Di Iorio, Seraphine Valeska Wegner, Eva Brand
Summary: This study generated a patient-derived antibody mixture as a reference for quantification of ADA titers, showing that ADAs from patients with FD have comparable affinities to agalsidase-alpha, agalsidase-beta and Moss-AGAL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Delong Hou, Liping Pu, Shuai Zhou, Rui Wang, Yong Xu, Wenle Zhang, Zhonghui Wang, Qi Zeng, Zhou Xu, Haojun Fan, Yi Chen
Summary: By engineering cucurbit[6]uril with spiropyran pendants, large 2D materials are synthesized inside living cells, allowing for potent intracellular signaling or theranostic platform that exceeds the performance of conventional small counterparts.
Article
Immunology
Vitoria Tavares Castro, Helene Chardin, Juliana Amorim dos Santos, Gustavo Barcelos Barra, Grazielle Rodrigues Castilho, Paula Monteiro Souza, Perola de Oliveira Magalhaes, Ana Carolina Acevedo, Eliete Neves Silva Guerra
Summary: This longitudinal study evaluated the detection of SARS-CoV-2 antibodies in the serum and saliva of COVID-19-vaccinated adults. The study found that saliva samples can be used to monitor antibody levels after vaccination, and heterologous vaccination can contribute to increased antibody levels.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Hui Wang, Daijie Chen, Huili Lu
Summary: Infectious diseases were a major cause of death prior to the 19th century, but the development of antibiotics and vaccines has greatly increased human life expectancy. However, the emergence of antibiotic-resistant superbugs poses new challenges. The side effects of broad-spectrum antibiotics and the lag in developing new antibiotics limit their effectiveness in treating infections. Monoclonal antibodies have shown promise in treating infectious diseases through various mechanisms, but further research and development are needed.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Computer Science, Information Systems
Yuhao Niu, Lin Gu, Yitian Zhao, Feng Lu
Summary: In this study, we propose an interpretable deep learning approach for medical diagnosis. By isolating and visualizing neuron activation patterns, we can determine the symptoms that the deep learning model uses for prediction. We also introduce a new network, Patho-GAN, to synthesize medically plausible images, and demonstrate that we can manipulate the generated images to control the properties of lesions. Our approach is faster and produces higher-quality images than previous methods, and has the potential to be an effective solution for data augmentation.
IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Xinyue Zhou, Mengjia Zhang, Hanyu Zhang, Hailong Ma, Jiaru Zhou, Hua Cao, Guanghao Guo, Ningning Ma, Qigai He, Yongle Yang, Yifei Lang, Yaowei Huang, Wentao Li
Summary: This study successfully generated and purified six monoclonal antibodies specifically targeting the spike protein of SADS-CoV. Three of these antibodies were capable of neutralizing SADS-CoV infection, and it was found that the hemagglutination phenomenon induced by SADS-CoV could be effectively suppressed by monoclonal antibodies specifically targeting the S1A domain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ophthalmology
Carmen L. Avendano-Monje, Miguel Cordero-Coma, Jose L. Mauriz, Sara Calleja-Antolin, Alex Fonollosa, Ana Garrote Llorden, Juan Martin Garcia-Sancho, Maria Sanchez-Salazar, Jose G. Ruiz de Morales
Summary: The study aimed to measure, characterize, and evaluate the clinical significance of anti-retinal antibodies in patients with sarcoid uveitis. The results showed that anti-retinal antibodies recognizing carbonic anhydrase II are common in sarcoid uveitis. Although not fully sensitive and specific, they might be a useful non-invasive diagnostic tool for the diagnosis of sarcoid uveitis.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2022)
Review
Biotechnology & Applied Microbiology
Maria Celeste Rodriguez, Diego Fontana, Ernesto Garay, Claudio Prieto
Summary: Rabies is a fatal disease transmitted to humans primarily through bites from infected animals, mainly dogs. Mass vaccination campaigns and rabies virus-neutralizing antibodies play crucial roles in eradicating dog-mediated human rabies by 2030. Despite the efficiency of current gold standard tests, such as RFFIT and FAVN, they have drawbacks in terms of standardization and requirements for live virus and specialized facilities. Alternative serological testing methods, including novel approaches and antigen binding methodologies, offer faster and potentially less complex options for measuring rabies glycoprotein antibodies. Validation and standardization procedures are important for improving rabies serology tests and their accuracy.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Ophthalmology
Shaun Sebastian Sim, Chee Wai Wong, Quan Hoang, Shu Yen Lee, Tien Yin Wong, Chui Ming Gemmy Cheung
Summary: The study found that ARAs are prevalent in highly myopic patients, with anti-CAII being particularly common in those with severe MMD, suggesting a potential association between ARAs and MMD. The presence of ARAs increases with longer AL.
Article
Immunology
Mo Zhou, Jun Xie, Osamu Kawase, Yoshifumi Nishikawa, Shengwei Ji, Shanyuan Zhu, Shinuo Cao, Xuenan Xuan
Summary: This study found that there were significant anti-erythrocyte and anti-platelet autoantibodies in mice infected with Plasmodium and Babesia parasites, which may contribute to thrombocytopenia and hemolytic anemia. This research will help to understand the mechanisms of malaria and babesiosis-related thrombocytopenia and hemolytic anemia.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jinrui Zhang, Ge Zhang, Wenjing Zhang, Lu Bai, Luning Wang, Tiantian Li, Li Yan, Yang Xu, Dan Chen, Wenting Gao, Chuanzhou Gao, Chaoqun Chen, Menglin Ren, Yuexia Jiao, Hongqiang Qin, Yu Sun, Lili Zhi, Yangfan Qi, Jinyao Zhao, Quentin Liu, Han Liu, Yang Wang
Summary: The RNA binding protein RBMS1 is prevalent in immune-cold triple-negative breast cancers (TNBC) and plays a role in regulating the stability of programmed death ligand 1 (PD-L1). Depletion of RBMS1 enhances cytotoxic T cell mediated anti-tumor immunity and combination with immune checkpoint blockade or CAR-T treatment further enhances anti-tumor T-cell immunity. Our findings provide a new immunotherapeutic strategy targeting the immunosuppressive RBMS1 in TNBC.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Engineering, Biomedical
Yuehang Zhang, Lei Yu, Renjie Qiu, Lisha Cao, Genlan Ye, Rurong Lin, Yongqin Wang, Guobao Wang, Bianxiang Hu, Honghao Hou
Summary: A novel biocompatible 3D porous hydrogel (DFO-gel) with sustained release capacity of hypoxia mimicking micromolecule drug deferoxamine (DFO) was developed for in-situ renal injury repair. The developed DFO-gels can scavenge excess reactive oxygen species (ROS), regulate inflammatory microenvironment, and promote angiogenesis, thereby effectively promoting renal injury repair. This multifunctional hydrogel not only exhibits sustained release and promoted biouptake capacity for DFO, but also improves the renal injured microenvironment by alleviating oxidative and inflammatory stress and promoting neovascularization.
MATERIALS TODAY BIO
(2023)
Review
Immunology
P. Decker, T. Moulinet, F. Pontille, M. Cravat, M. De Carvalho Bittencourt, R. Jaussaud
Summary: Anti-Ro52 antibodies are commonly found in patients with connective tissue diseases and are associated with certain clinical features and survival. They may have a role in the inflammatory response.
AUTOIMMUNITY REVIEWS
(2022)
Article
Endocrinology & Metabolism
Merrick S. Reynolds, Chad R. Hancock, Jason D. Ray, Kyle B. Kener, Carrie Draney, Kevin Garland, Jeremy Hardman, Benjamin T. Bikman, Jeffery S. Tessem
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2016)
Article
Biochemistry & Molecular Biology
Jason D. Ray, Kyle B. Kener, Benjamin F. Bitner, Brent J. Wright, Matthew S. Ballard, Emily J. Barrett, Jonathon T. Hill, Larry G. Moss, Jeffery S. Tessem
Article
Biochemistry & Molecular Biology
Thomas J. Rowley, Benjamin F. Bitner, Jason D. Ray, Daniel R. Lathen, Andrew T. Smithson, Blake W. Dallon, Chase J. Plowman, Benjamin T. Bikman, Jason M. Hansen, Melanie R. Dorenkott, Katheryn M. Goodrich, Liyun Ye, Sean F. O'Keefe, Andrew P. Neilson, Jeffery S. Tessem
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Benjamin F. Bitner, Jason D. Ray, Kyle B. Kener, Jacob A. Herring, Josie A. Tueller, Deborah K. Johnson, Claudia M. Tellez Freitas, Dane W. Fausnacht, Mitchell E. Allen, Alexander H. Thomson, K. Scott Weber, Ryan P. McMillan, Matthew W. Hulver, David A. Brown, Jeffery S. Tessem, Andrew P. Neilson
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Erin Q. Jennings, Jason D. Ray, Christopher J. Zerio, Marissa N. Trujillo, David M. McDonald, Eli Chapman, David A. Spiegel, James J. Galligan
Summary: Post-translational modifications play important roles in physiological and pathophysiological processes, and a novel PTM called lactoylLys has been identified. Sirtuin 2 has been found to control the abundance of lactoylLys, addressing a gap in understanding how nonenzymatic PTMs are regulated.