期刊
ACS APPLIED MATERIALS & INTERFACES
卷 12, 期 42, 页码 47840-47847出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c13003
关键词
adenosine triphosphate (ATP); zeolitic imidazolate frameworks; NIR fluorescence nanoprobe; biological imaging; colitis
资金
- National Natural Science Foundation of China [21775133, 21874114]
- Hunan Provincial Natural Science Foundation [2018JJ2385]
- Scientific Research Fund of Hunan Provincial Education Department [19A479]
- Degree & Postgraduate Education Reform Project of Hunan Province [CX20190483]
Adenosine triphosphate (ATP) is mainly produced in mitochondria and plays an important role in lots of pathological processes such as colitis. Unfortunately, to date, few suitable fluorescence probes have been developed for monitoring the ATP level in colitis. Herein, a fluorescence nanoprobe named NIR@ZIF-90 is proposed and prepared by encapsulating a rhodamine-based near-infrared (NIR) dye into zeolitic imidazolate frameworks (ZIF-90). The nanoprobe is nonfluorescent because the emission of NIR is suppressed by the encapsulation, while in the presence of ATP, the framework of ZIF-90 is dissembled to release NIR and thus NIR fluorescence at 750 nm is observed. The nanoprobe shows high sensitivity to ATP with a 72-fold increase and excellent selectivity to ATP over other nucleotides. Moreover, with low cytotoxicity and good mitochondria-targeted ability, NIR@ZIF-90 is used to image ATP in colorectal cancer cells (HCT116). In addition, due to the NIR emission, the nanoprobe is further employed to successfully monitor the ATP level in a colitis mouse model. To the best of our knowledge, the nanoprobe is the first example to study colitis in vivo with the guidance of ATP, which will provide an efficient tool for understanding colitis.
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