期刊
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
卷 9, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s40164-020-00168-0
关键词
Cancer stem cells; Prostate cancer; Sphere; PI3K; AKT; Histone acetylation
资金
- First Affiliated Hospital of Zhengzhou University, Zhengzhou
- Henan Province People's Hospital, Zhengzhou University, Zhengzhou, Henan, China (Henan Provincial Project Grant for Basic Research and Frontier Technology) [142300410325, 152300410154]
Background Human prostate cancer spheres endowed with stem cell properties have been obtained from androgen-dependent cell line LNCaP after exposure to an epigenomic modulator phenethyl isothiocynate (PEITC). Sphere cells can self-renew and grow with androgen, and also without androgen. Little is known about the signaling pathway and mechanism in the development of the stem cells in the spheres. Methods Expression of phosphoinositol-3 kinase (PI3K) pathway members and histone acetylation were quantified in the tumor spheres and LNCaP cells by western immunoblotting. Results The level of phosphorylated AKT was significantly increased in the sphere stem cells than the LNCaP cells at an average of 7.4 folds (range 5.8-10.7 folds), whereas the P27 level was elevated 5.4 folds (range 4.8-6.3 folds) (P < 0.05). The acetylation level on histone H3 lysine 9 was decreased. Conclusions PEITC appears to regulate the epigenome through histone acetylation and activate the PI3K/AKT pathway in the LNCaP cells. This mechanism may be responsible in part for the development of the prostate cancer stem cells.
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