期刊
BIOLOGY-BASEL
卷 9, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/biology9060119
关键词
arachidonic acid; bacterial community; dihomo-gamma-linolenic acid; fish; intestine; immunity; immune function; microbiome
类别
资金
- U.S. Agency for International Development, Mid-East regional Cooperation (MERC) Projects [M28-061, M33-034]
- Jewish Charitable Association (JCA), Israel
- Israeli Council for Higher Education, Planning and Budgeting Committee (PBC) program
The effect of dietary omega-6 long-chain polyunsaturated fatty acid (LC-PUFA) on host microbiome and gut associated immune function in fish is unexplored. The effect of dietary supplementation with the omega-6 LC-PUFA-rich microalgaLobosphaera incisawild type (WT) and its delta-5 desaturase mutant (MUT), rich in arachidonic-acid and dihomo-gamma-linolenic acid (DGLA), respectively, on intestinal gene expression and microbial diversity was analyzed in zebrafish. For 1 month, fish were fed diets supplemented with broken biomass at 7.5% and 15% (w/w) of the twoL. incisastrains and a control nonsupplemented commercial diet. Dietary supplementation resulted in elevated expression of genes related to arachidonic acid metabolism-cyclooxygenase 2 (cox-2), lipoxygenase 1(lox-1), anti-inflammatory cytokine-interleukin 10 (il-10), immune defense-lysozyme (lys), intestinal alkaline phosphatase (iap), complement (c3b), and antioxidants-catalase (cat), glutathione peroxidase (gpx). Microbiome analysis of the gut showed higher diversity indices for microbial communities in fish that were fed the supplemented diets compared to controls. Different treatment groups shared 237 operational taxonomic units (OTUs) that corresponded to the core microbiome, and unique OTUs were evident in different dietary groups. Overall, the zebrafish gut microbiome was dominated by the phylum Fusobacteria and Proteobacteria (averaging 38.4% and 34.6%, respectively), followed by Bacteroidetes (12.9%), Tenericutes, Planctomycetes, and Actinobacteria (at 3.1-1.3%). Significant interaction between some of the immune-related genes and microbial community was demonstrated.
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