Review
Oncology
Krzysztof Kotowski, Jakub Rosik, Filip Machaj, Stanislaw Supplitt, Daniel Wiczew, Karolina Jablonska, Emilia Wiechec, Saeid Ghavami, Piotr Dziegiel
Summary: Glycolysis is a crucial metabolic process in rapidly proliferating cells, such as cancer cells, with PFK-1 being a key rate-limiting enzyme regulated primarily by F-2,6-BP. PFK-2/FBPase-2 is a bifunctional enzyme responsible for F-2,6-BP synthesis and degradation, and alterations in PFKFB isozymes play important roles in various diseases, particularly in cancer.
Article
Biochemistry & Molecular Biology
Beth A. Griesel, Satoshi Matsuzaki, Albert Batushansky, Timothy M. Griffin, Kenneth M. Humphries, Ann Louise Olson
Summary: This study using 3T3-L1 adipocytes as a model system found that an increase in glucose uptake during the first 3 days of differentiation promoted the expansion of the adipocyte metabolome and proteome. The positive effects were specifically due to increased expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), highlighting its critical role in regulating cellular metabolic remodeling for adipocyte differentiation and maturation.
Article
Food Science & Technology
Surinder S. Chauhan, Michelle LeMaster, Eric M. England
Summary: PFK-1 activity in porcine skeletal muscle under postmortem conditions is regulated by F-2,6-BP and AMP, with AMP potentially playing a greater role in determining the rate and extent of postmortem muscle glycolysis and pH decline.
Article
Biochemistry & Molecular Biology
Jorge Duarte-Gutierrez, Alma B. Peregrino-Uriarte, Silvia Gomez-Jimenez, Veronica Mata-Haro, Gloria Yepiz-Plascencia
Summary: The study investigated the impact of silencing HIF-1 on MT expression and apoptosis in Litopenaeus vannamei under different oxygen conditions. Results showed differential regulation of MT expression and apoptosis by HIF-1 in various tissues during normoxia, hypoxia, and reoxygenation.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Susan Wai Sum Leung, Yi Shi
Summary: Endothelial cells play a crucial role in regulating vascular tone and maintaining homeostasis. Cell metabolism, especially glycolysis, is essential for cell function and response to stimuli. Discussions on the role of glycolysis in cardiovascular disease may provide insights for future therapeutic strategies.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Oncology
Kreon Koukoulas, Antonis Giakountis, Angeliki Karagiota, Martina Samiotaki, George Panayotou, George Simos, Ilias Mylonis
Summary: ERK-mediated phosphorylation of HIF-1 alpha enhances its interaction with NPM1, which influences the transcriptional activation of HIF-1 target genes. NPM1 and HIF-1 co-regulate genes enriched in different cancer types, and their expression correlates with hypoxic tumor status and patient prognosis.
MOLECULAR ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mandy Pack, Tim Nikolai Gulde, Michelle Victoria Voelcker, Anne S. Boewe, Selina Wrublewsky, Emmanuel Ampofo, Mathias Montenarh, Claudia Goetz
Summary: Glucose homeostasis is vital for organism survival and is regulated by CK2. In this study, a qRT-PCR array was used to identify diabetes-associated genes and it was found that inhibition of CK2 led to decreased gene expression and activity of fructose-1,6-bisphosphatase 1 (FBP1), a central enzyme in gluconeogenesis. As a result, glucose secretion was reduced. These findings highlight FBP1 as a new factor in the CK2-regulated network of carbohydrate metabolism control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Laura E. Hernandez-Aguirre, Alma B. Peregrino-Uriarte, Jorge L. Duarte-Gutierrez, Lilia Leyva-Carrillo, Josafat M. Ezquerra-Brauer, Elisa M. Valenzuela-Soto, Gloria Yepiz-Plascencia
Summary: Animals, including the white shrimp Penaeus (Litopenaeus) vannamei, experience hypoxia when their oxygen consumption exceeds oxygen availability. In this study, researchers investigated how shrimp cope with hypoxia by regulating enzymes involved in energy production, specifically glucose-6-phosphatase (G6Pase). They identified two isoforms of the G6Pase gene and found that their expression is influenced by the transcription factor HIF-1 in response to oxygen deprivation and reoxygenation.
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
(2023)
Article
Multidisciplinary Sciences
Mengdong Wang, Di Wang, Yue Lang, Anwen Shao, Rui Zhang, Jun Tang, Dongming Lai, Chenglu Xiao
Summary: HIF-1α is crucial for hypoxia response by upregulating gene expression. L3MBTL3, regulated by HIF-1α, interacts with and promotes the degradation of HIF-1α in the nucleus, forming a negative feedback loop to dampen the hypoxic response.
Article
Cell Biology
Chao Lu, Pengyun Qiao, Ruihai Fu, Yadi Wang, Jiayi Lu, Xi Ling, Lu Liu, Yujun Sun, Chune Ren, Zhenhai Yu
Summary: Endometriosis is a common cause of chronic pelvic pain and infertility in women. Our study reveals that PIM2 mediates the phosphorylation of PFKFB4, promoting glycolysis and cell growth in the progression of endometriosis. This research provides new insights into the reprogramming of glucose metabolism in endometriosis and potential non-contraceptive treatments.
CELL DEATH & DISEASE
(2022)
Article
Biology
Meghane Sittewelle, Vincent Kappes, Chenxi Zhou, Deborah Lecuyer, Anne H. Monsoro-Burq
Summary: This study reveals a novel function of PFKFB4 in melanoma cell migration by promoting ICMT/RAS interaction, controlling RAS localization at the plasma membrane, activating AKT signaling, and enhancing cell migration.
LIFE SCIENCE ALLIANCE
(2022)
Review
Cell Biology
Nasim Kheshtchin, Jamshid Hadjati
Summary: Hypoxia, a common characteristic of solid tumors, contributes to different aspects of tumor progression and limits the efficacy of immunotherapies. Developing new immunotherapy strategies involving therapeutic targeting of HIF-1 molecules associated with hypoxia may enhance the clinical effectiveness of immunotherapy. Targeting hypoxia presents a potential opportunity to improve the clinical benefit of cancer immunotherapy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marissa Flores-Sauceda, Laura Camacho-Jimenez, Alma B. Peregrino-Uriarte, Lilia Leyva-Carrillo, Aldo Arvizu-Flores, Gloria Yepiz-Plascencia
Summary: Hypoxia is a common stressor in marine environments, impacting marine species negatively. White shrimp Litopenaeus vannamei adapt to hypoxic conditions by activating tissue-specific changes in glycolytic and gluconeogenic enzymes. The control of glycolytic/gluconeogenic fluxes by fructose-2,6-bisphosphate levels and the negative regulation of PFKFBP1-2 expression in response to severe hypoxia are crucial for anaerobic glycolysis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sho Koyasu, Shoichiro Horita, Keisuke Saito, Minoru Kobayashi, Hiroshi Ishikita, Christalle C. T. Chow, Gouki Kambe, Shigeto Nishikawa, Toshi Menju, Akiyo Morinibu, Yasushi Okochi, Yoshiaki Tabuchi, Yasuhito Onodera, Norihiko Takeda, Hiroshi Date, Gregg L. Semenza, Ester M. Hammond, Hiroshi Harada
Summary: p53 deficiency triggers the activation of HIF-1 and ZBTB2 plays a crucial role in this process. ZBTB2 promotes invasion and growth of p53-deficient cancers and is associated with poor prognosis in lung cancer patients. ZBTB2 N-terminus-mimetic polypeptides inhibit ZBTB2 activity and suppress tumor aggressiveness.
Article
Genetics & Heredity
Nan Niu, Hui Li, Xiancai Du, Chan Wang, Junliang Li, Jihui Yang, Cheng Liu, Songhao Yang, Yazhou Zhu, Wei Zhao
Summary: This study found that NRF-1 and HIF-1α are important anti-apoptotic molecules in cardiomyocytes under hypoxia, although their regulatory mechanisms differ. NRF-1 exerts its anti-apoptotic effect by negatively regulating the expression of HIF-1α.
Article
Ophthalmology
Fatima Sofia Magana-Guerrero, Diana Hinojosa Trujillo, Beatriz Buentello-Volante, Jose Eduardo Aguayo-Flores, Edgar Alonso Melgoza-Gonzalez, Jesus Hernandez, Maria C. Jimenez-Martinez, Sonia Mayra Perez-Tapia, Yonathan Garfias
Summary: During the COVID-19 pandemic, healthcare workers are at a high risk of being infected with SARS-CoV-2, especially those in ophthalmology departments. A study conducted in Mexico City found that 32% of HCWs at an Ophthalmological Reference Centre tested RT-PCR positive, with 20% presenting IgG antibodies against SARS-CoV-2. Despite the high infection rate, most HCWs showed mild symptoms, and a significant portion of RT-PCR positive participants did not show IgG antibodies.
OPHTHALMIC EPIDEMIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marissa Flores-Sauceda, Laura Camacho-Jimenez, Alma B. Peregrino-Uriarte, Lilia Leyva-Carrillo, Aldo Arvizu-Flores, Gloria Yepiz-Plascencia
Summary: Hypoxia is a common stressor in marine environments, impacting marine species negatively. White shrimp Litopenaeus vannamei adapt to hypoxic conditions by activating tissue-specific changes in glycolytic and gluconeogenic enzymes. The control of glycolytic/gluconeogenic fluxes by fructose-2,6-bisphosphate levels and the negative regulation of PFKFBP1-2 expression in response to severe hypoxia are crucial for anaerobic glycolysis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Cell Biology
Ricardo Gonzalez-Ruiz, Lilia Leyva-Carrillo, Alma B. Peregrino-Uriarte, Gloria Yepiz-Plascencia
Summary: This study analyzed the effects of high temperature, hypoxia, reoxygenation, and the combination of these factors on the gene expression in the gills of Litopenaeus vannamei. The results showed that HSF1, anaerobic metabolism, and the pentose phosphate pathway (PPP) play crucial roles in the shrimp's response to these abiotic stress conditions and contribute to their survival.
CELL STRESS & CHAPERONES
(2023)
Article
Biochemistry & Molecular Biology
Paulina Estrada-Cardenas, Laura Camacho-Jimenez, Alma B. Peregrino-Uriarte, Carmen A. Contreras-Vergara, Jorge Hernandez-Lopez, Gloria Yepiz-Plascencia
Summary: This study analyzed the GPx4 gene in Litopenaeus vannamei and evaluated its responses to hypoxia and p53 knock-down. The findings suggest that GPx4 may serve as a sensitive indicator of antioxidant imbalance during hypoxia and that p53 modulates GPx4 responses during stress. The study also highlights the effectiveness of the antioxidant response in preventing protein damage during hypoxia.
Article
Biochemistry & Molecular Biology
Omar N. Granillo-Luna, Laura E. Hernandez-Aguirre, Alma B. Peregrino-Uriarte, Jorge Duarte-Gutierrez, Carmen A. Contreras-Vergara, Teresa Gollas-Galvan, Gloria Yepiz-Plascencia
Summary: Hypoxic zones are spreading worldwide in marine environments, affecting many organisms. Shrimp and other marine crustaceans have the ability to withstand hypoxia through metabolic adaptations. This study characterized the pyruvate carboxylase (PC) gene in the white shrimp Litopenaeus vannamei and found tissue-specific expression in the hepatopancreas, indicating its key role in energy production even under hypoxic conditions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Immunology
Noe Juvenal Mendoza-Ramirez, Julio Garcia-Cordero, Sandra Paola Martinez-Frias, Daniela Roa-Velazquez, Rosendo Luria-Perez, Jose Bustos-Arriaga, Jesus Hernandez-Lopez, Carlos Cabello-Gutierrez, Joaquin Alejandro Zuniga-Ramos, Edgar Morales-Rios, Sonia Mayra Perez-Tapia, Martha Espinosa-Cantellano, Leticia Cedillo-Barron
Summary: This study analyzes the combination of two SARS-CoV-2 viral antigens and finds that immunization with a combination of S1 or RBD and the N protein can elicit a stronger immune response. The sera from immunized mice can recognize different variants of SARS-CoV-2. This study identifies potential antigens for the second generation of COVID-19 vaccines.
Article
Microbiology
Freddy Dehesa-Canseco, Roxana Pastrana-Unzueta, Nadia Carrillo-Guzman, Francisco Liljehult-Fuentes, Juan Diego Perez-De la Rosa, Humberto Ramirez-Mendoza, Jose Guillermo Estrada-Franco, Roberto Navarro-Lopez, Jesus Hernandez, Mario Solis-Hernandez
Summary: This study aimed to evaluate the seroprevalence of neutralizing antibodies against the ancestral strain and the Omicron BA.1 subvariant in dogs and cats in Mexico. The results showed that 14.2% of cats and 1.5% of dogs presented nAbs against the ancestral strain of SARS-CoV-2, while a lower proportion had nAbs against Omicron BA.1.
Article
Biochemistry & Molecular Biology
Paulina Estrada-Cardenas, Alma B. Peregrino-Uriarte, Silvia Gomez-Jimenez, Elisa M. Valenzuela-Soto, Lilia Leyva-Carrillo, Gloria Yepiz-Plascencia
Summary: Despite little knowledge about the interrelationships among GPx isoforms in shrimp, GPx2 and GPx4 exhibit similar expression patterns during hypoxia and reoxygenation. GPx4 knock-down down-regulates GPx2 expression, suggesting that GPx4 affects GPx2 expression. Total GPx activity changes during hypoxia and reoxygenation, while GPx4 activity is unaffected by any stressor. The GSH/GSSG ratio in hepato-pancreas remains well-modulated at early hours but is impaired at 12 hours due to hypoxia and reoxygenation.
Article
Nursing
Maria Josse Navarro-Ibarra, Maria del Socorro Saucedo-Tamayo, Heliodoro Aleman-Mateo, Hector Parra-Sanchez, Paola Othon-Ontiveros, Jesus Hernandez, Graciela Caire-Juvera
Summary: This study found that body fat percentage and fat mass index were positively associated with C-reactive protein (CRP) levels in female breast cancer survivors, while appendicular skeletal lean mass index (ASMI) decreased as CRP levels increased. Interleukin 6 (IL-6) was positively associated with fat mass index. Interventions to improve body composition in breast cancer survivors should consider the role of inflammatory markers to avoid sarcopenic obesity and the risk of recurrence.
BIOLOGICAL RESEARCH FOR NURSING
(2023)
Article
Multidisciplinary Sciences
Tania Lucia Madrigal-Valencia, Manuel Saavedra-Montanez, Armando Perez-Torres, Jesus Hernandez, Joaquim Segales, Yesmin Dominguez Hernandez, Irma Eugenia Candanosa-Aranda, Alfredo Perez-Guiot, Humberto Ramirez-Mendoza
Summary: This study describes an outbreak of sheep-associated malignant catarrhal fever (SA-MCF) caused by OvHV-2 in Mexico. The virus was identified using various methods and observed in primary rabbit testis cultures. It is also the first report of OvHV-2 presence in horses and Artiodactyla buffy coats in Mexico.
Letter
Biochemistry & Molecular Biology
Jesus Hernandez, Freddy Dehesa-Canseco, Alma B. Vazquez-Lopez, Monica Resendiz-Sandoval, Graciela Caire-Juvera, Mario Solis-Hernandez, Olivia Valenzuela, Bruno Gomez-Gil, Veronica Mata-Haro
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Virology
Monica Resendiz-Sandoval, Veronica A. Vazquez-Garcia, Kenneth Contreras-Vega, Edgar A. Melgoza-Gonzalez, Veronica Mata-Haro, Luis Gimenez-Lirola, Jesus Hernandez
Summary: This study investigated the presence of PCV3 in samples collected in Mexico and found that PCV3 has been circulating in the region since 2008. The virus has a higher prevalence in pigs with clinical manifestations of diseases.
Article
Agriculture, Dairy & Animal Science
Maritza Cordero-Ortiz, Monica Resendiz-Sandoval, Freddy Dehesa-Canseco, Mario Solis-Hernandez, Jahir Perez-Sanchez, Carlos Martinez-Borges, Veronica Mata-Haro, Jesus Hernandez
Summary: Summary: This study aimed to develop a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to detect SARS-CoV-2 antibodies in serum samples from various animal species. The results showed that this assay was able to detect antibodies against SARS-CoV-2 in serum samples from cats, dogs, guinea pigs, rats, tigers, and humans.
Article
Biochemistry & Molecular Biology
Sang Hyuck Lee, Mi Ae Kim, Young Chang Sohn
Summary: This study characterized the CCAP signaling system in Pacific abalone and revealed its relationship with the calcium/PKC and cAMP/PKA signal transduction pathways. It provides new insights into the evolutionary origins of the NPS and OT/VP signaling systems in protostomes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Hanna Scheuffele, Erica V. Todd, John A. Donald, Timothy D. Clark
Summary: Global warming is causing an increase in extreme weather events, affecting ecosystems and the behavior of ectotherms. More research is needed to understand the impact of diurnal thermal variability and improve climate change predictions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jaakko Haverinen, Ahmed Badr, Markus Eskelinen, Matti Vornanen
Summary: The acclimatization of certain ectothermic vertebrates to winter conditions involves reduced energy consumption, achieved through decreased movement activity, depression of cellular functions, or switching to anaerobic energy production. This study on crucian carp showed that winter-acclimatized fish exhibited lower metabolic rates and a shift to anaerobic energy production during anoxia. Winter dormancy in crucian carp is achieved through active reduction of energy consumption, a slowed metabolic rate, and a direct response to oxygen absence.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Maria Cruz Sueiro, Cynthia A. Awruch, Gustavo M. Somoza, Walter S. Svagelj, Maria G. Palacios
Summary: According to life-history theory, there might be different immunological strategies between reproductive and non-reproductive periods due to limited resources. Our study on two sympatric marine fish species, rockfish and sandperch, found that reproductive individuals showed lower immune function, but higher levels of natural antibodies in rockfish females, compared to their non-reproductive counterparts. On the other hand, reproductively active sandperch males showed lower levels of natural antibodies and higher neutrophil to lymphocyte ratio and spleen index compared to non-reproductive males. The study highlights the species-specific patterns of immunity and the potential influence of resource limitation and abiotic factors on immune trade-offs in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
James B. Cummins, Dane A. Crossley II
Summary: This study focuses on cardiovascular development in altricial bird species, which has been less studied compared to precocial species. The researchers investigated the cholinergic and adrenergic receptor mediated control of arterial blood pressure and heart rate in the neotropic cormorant. The findings suggest that both cholinergic and adrenergic tone play a role in cardiovascular regulation in embryonic birds.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ru Xia, Hong-Kang Liu, Xi-Feng Liu, Xin Deng, Chuan-Jie Qin, Yuan-Fa He, Shi-Mei Lin, Yong-Jun Chen
Summary: This study cloned the genes encoding glucokinase and glucose-6-phosphatase catalytic subunit in largemouth bass, revealing their distribution and transcriptional regulation. The results showed that the functions of G6pc and Gck in LMB were highly conserved in evolution, and the interconversion of glucose and G6P in the liver was well regulated at the transcript level under high starch diet, but a futile cycle was induced after a glucose load.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)